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931.
932.
Leukemic cells cultured in the presence of various conditioned media differentiate into macrophages. This finding suggested that the maintenance of undifferentiated state and self-renewal in vivo may be related to the inability of the host to generate an appropriate level of differentiation factor (DF). Evidence for this hypothesis was derived from experiments in vitro and in vivo with myeloid leukemia of rat. The following results were obtained: (i) in vitro, the percentage of cell differentiation at a fixed concentration of DF was inversely related to the concentration of cells; (ii) leukemic cell inoculates that were lethal to 7-day-old rats were rejected by 21-day-old rats; (iii) leukemic cells in diffusion chambers underwent differentiation in 21-day-old rats but not in 7-day-old rats; (iv) organs from 21-day-old rats contained more DF activity than those of 7-day-old rats; (v) treatment of rats with DF in diffusion chambers resulted in leukemic cell differentiation inside the chamber; and (vi) the development of leukemia in 7-day-old rats was aborted by treatment with DF. These results show that the differentiation of rat leukemia cells requires the appropriate level of DF. The proliferation of transplanted leukemia cells in 7-day-old rats goes unchecked because of inadequate generation of DF. Conversely, in the 21-day-old rats, rejection is accomplished by differentiation of the transplanted cells.  相似文献   
933.
Simple phenolic acid levels were determined on pooled millstreams of five different classes of Canadian wheat milled to ~75, 80, and 85% extraction. Pooled flours and whole grain were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) to establish endogenous levels of insoluble bound, soluble esterified, and free phenolic acids. Only ferulic acid was detected in the insoluble bound category, which accounted for >80% of the total phenolic acids present in every flour. The soluble esterified phenolic acids accounted for up to 17% of the overall total phenolic acid content within a flour. The major constituents were sinapic, ferulic, and vanillic acids, with minor amounts of coumaric, caffeic, and syringic acids. Free phenolic acids accounted for a maximum of 6% of the total phenolic content of any prepared flour. Ferulic acid was the major free phenolic acid, while sinapic acid was not detected in any flour. Significant correlations (r = 0.64–0.97, P < 0.05) were observed between insoluble bound ferulic acid, individual soluble esterified acids, and most free acids with polyphenol oxidase activity, as well as color and ash content for each class.  相似文献   
934.
Predatory bacteria remain molecularly enigmatic, despite their presence in many microbial communities. Here we report the complete genome of Bdellovibrio bacteriovorus HD100, a predatory Gram-negative bacterium that invades and consumes other Gram-negative bacteria. Its surprisingly large genome shows no evidence of recent gene transfer from its prey. A plethora of paralogous gene families coding for enzymes, such as hydrolases and transporters, are used throughout the life cycle of B. bacteriovorus for prey entry, prey killing, and the uptake of complex molecules.  相似文献   
935.
Magnetic resonance (MR) imaging characteristics of intracranial granular cell tumors (GCTs) have been previously reported in three dogs. The goal of this retrospective study was to examine a larger number of dogs and determine whether distinctive MR characteristics of intracranial GCTs could be identified. Six dogs with histologically confirmed intracranial GCTs and MR imaging were included. Tumor location, size, mass effect, T1‐ and T2‐weighted signal intensity, and peritumoral edema MR characteristics were recorded. In all dogs, GCTs appeared as well‐defined, extra‐axial masses with a plaque‐form, sessile distribution involving the meninges. All tumors were located along the convexity of the cerebrum, the falx cerebri, or the ventral floor of the cranial vault. All tumors were mildly hyperintense on T1‐weighted images, and iso‐ to hyperintense on T2‐weighted images. A moderate‐to‐severe degree of peritumoral edema and mass effect were evident in all dogs. Findings indicated that, while several MR imaging characteristics were consistently identified in canine cerebral GCTs, none of these characteristics were unique or distinctive for this tumor type alone.  相似文献   
936.
Feather-picking and self-mutilation behaviors are common in psittacine birds. These behaviors are best defined as stereotypic behaviors or obsessive compulsive disorders. There is likely a genetic predisposition for these behaviors as reflected in the overrepresentation of a number of species of psittacines. Stereotypies most often result from a high level of arousal and the inability to respond with the appropriate natural behaviors. More specifically, conflict may be induced by the absence of releasing stimuli or target objects. On a chemical level, stereotypic behavior appears to be related to an increased dopaminergic activity and an increased dopamine turnover. Feather-picking and self-mutilation patients are challenging to treat and are most likely to respond to treatment at an early stage of development. The best results are obtained by identifying and removing the cause of conflict, enhancing the environment, using appropriate drugs when indicated, and employing counter-conditioning.  相似文献   
937.
In order to evaluate the seroconversion of horses to Babesia caballi and B. canis in Hungary, blood samples were collected from 371 animals on 23 different locations of the country. The presence of antibodies to B. caballi was screened with a competitive ELISA. All 29 positive samples came from one region (the Hortobágy). The prevalence of infection did not show correlation with sexes, and reached 100% in the age group of 2-5 years. Babesia canis-specific antibodies were demonstrated by IFAT in 6.74% of animals kept in 7 regions. The titres were low or medium level (1:40 to 1:160), indicating that the horses had previously been exposed to this piroplasm, but their infection must have been limited. The highest seropositivity rate was observed in the age group of 3-4 years, and males (stallions and geldings) were significantly more frequently infected than females. However, neither B. caballi nor B. canis could be identified in the peripheral blood samples of infected horses by PCR. Since most of the B. caballi-positive horses remained negative in the B. canis IFAT, whereas seroconversion solely to B. canis was detected in several regions of the country, serological cross-reaction between the two species can be discounted. This is the first serological evidence of horses being naturally infected with B. canis, supporting the view that piroplasms are less host specific than previously thought.  相似文献   
938.
The analysis focused on model fitting of 2 ewe reproductive traits, litter size, and days to lambing (interval between the introduction of the ram into the flock and the subsequent parturition of the ewes). The experimental data set of the Universitat Autònoma of Barcelona flock was used, including 1,598 records of litter size and 1,699 records of days to lambing from 376 Ripollesa ewes between 1986 and 2005. Univariate and bivariate models were considered as beginning points with linear or threshold approximation for litter size. Model fitting was evaluated in terms of goodness-of-fit and predictive ability, using the mean square error and the correlation between phenotypic and predicted records (rho(y,?)) as reference parameters. The bivariate model was preferable for both variables, minimizing mean square error and maximizing rho(y,?). A threshold approximation for litter size was preferable over a linear approximation. Models were also compared with a simulation study, comparing the correlation coefficient between simulated and predicted breeding values (rho(a,a)). The bivariate threshold model was favored, with a rho(y,?) of 0.677 and 0.834 for litter size and days to lambing, respectively. Correlation coefficients between simulated and predicted breeding values in the bivariate linear model were reduced slightly to 0.651 and 0.831, respectively, and they were lowest with linear univariate models (0.642 and 0.802). Although the bivariate models for ewe litter size and days to lambing were more accurate than the univariate models, the threshold approaches showed a greater advantage under the bivariate model. For the purpose of genetic evaluation of litter size in sheep, use of the threshold-linear model seems justified. In the Ripollesa breed, the evaluation of litter size can benefit from recording birth weight.  相似文献   
939.
Further studies of the Saturnian magnetosphere and planetary magnetic field by Voyager 2 have substantiated the earlier results derived from Voyager 1 observations in 1980. The magnetic field is primarily that of a centered dipole (moment = 0.21 gauss-RS(3); where one Saturn radius, RS, is 60,330 kilometers) tilted approximately 0.8 degrees from the rotation axis. Near closest approach to Saturn, Voyager 2 traversed a kronographic longitude and latitude range that was complementary to that of Voyager 1. Somewhat surprisingly, no evidence was found in the data or the analysis for any large-scale magnetic anomaly in the northern hemisphere which could be associated with the periodic modulation of Saturnian kilometric radiation radio emissions. Voyager 2 crossed the magnetopause of a relatively compressed Saturnian magnetosphere at 18.5 RS while inbound near the noon meridian. Outbound, near the dawn meridian, the magnetosphere had expanded considerably and the magnetopause boundary was not observed until the spacecraft reached 48.4 to 50.9 RS and possibly beyond. Throughout the outbound magnetosphere passage, a period of 46 hours (4.5 Saturn rotations), the field was relatively steady and smooth showing no evidence for any azimuthal asymmetry or magnetic anomaly in the planetary field. We are thus left with a rather enigmatic situation to understand the basic source of Saturnian kilometric radiation modulation, other than the small dipole tilt.  相似文献   
940.
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