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Szczerbal I Stefaniak T Dubiel A Siembieda J Nizanski W Switonski M 《Veterinary pathology》2006,43(5):789-792
We report here on a case of a Holstein-Friesian male calf with the congenital total absence of thoracic limbs (amelia). Cytogenetic study showed a high rate of chromosome instability, represented by chromosome or chromatid breaks and gaps in 46% of the analyzed metaphase spreads. Moreover, 12% of the spreads appeared to be polypolid. The number of micronuclei also was significantly higher when compared to control animals. This paper discusses the association between chromosome instability and limb malformation. 相似文献
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I. Szczerbal J. Klukowska-Roetzler G. Dolf C. Schelling & M. Switonski 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(5):337-342
Cytogenetic mapping of the arctic fox and the Chinese raccoon dog were performed using a set of canine probes derived from the Bacterial Artificial Chromosome (BAC) library. Altogether, 10 BAC clones containing sequences of selected genes (PAX3, HBB, ATP2A2, TECTA, PIT1, ABCA4, ESR2, TPH1, HTR2A, MAOA) and microsatellites were mapped by fluorescence in situ hybridization (FISH) experiments to chromosomes of the canids studied. At present, the cytogenetic map on the arctic fox and Chinese raccoon dog consists of 45 loci each. Chromosomal localization of the BAC clones was in agreement with data obtained by earlier independent comparative chromosome painting. However, two events of telomere‐to‐centromere inversions were tentatively identified while compared with assignments in the dog karyotype. 相似文献
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Monika Bugno-Poniewierska Barbara Kij Wojciech Witarski Magdalena Wojtaszek Anna Radko Angelika Podbielska Izabela Szczerbal William J. Murphy 《Reproduction in domestic animals》2020,55(9):1139-1144
The tortoiseshell coat colour is characteristic to female cats, and its occurrence in tomcats is very rare and associated with chromosome abnormalities (additional copy of X chromosome). The aim of this study was identification of the genetic basis of a case of tortoiseshell colour in a fertile Maine coon tomcat. Cytogenetic and molecular genetic studies were carried out with painting molecular probes (WCPP) specific to the X and Y sex chromosomes as well as a DNA microsatellite panel for the parentage verification of cats. Cytogenetic analysis revealed only a single set of sex chromosomes typical for male – 38,XY. The results of the microsatellite polymorphism obtained from DNA showed three alleles in locus FCA201 and four alleles in loci FCA149 and FCA441 in different tissues (blood, hair roots and testicles). Based on these results, the case was diagnosed as a true chimerism 38,XY/38,XY. To the best of our knowledge, this is the first case of a 38,XY/38,XY chimera diagnosed in cats, confirmed by genetic analysis. 相似文献
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Morphological features of the seminiferous epithelium in cat (Felis catus, L. 1758) testes 总被引:1,自引:0,他引:1
The aim of the present study was to assess the changes in the germinal epithelium in cats of different ages. Routine histological staining was applied to perform morphological and stereological examinations. The animals were divided into five groups according to age: under 8 months (n=28), 8-12 months (n=30), 12-36 months (n=33), 3-6 years (n=14) and older than 6 years (n=13). The appearance of the gonads of the males in the first group varied the most. The seminiferous tubules of the youngest cats consisted of a monolayer of supporting cells and a few spermatogonia. No tubular lumina were present, and the diameters of the seminiferous tubules reached 132.5 microm. We noted the typical arrangement of gametogenic cells with a tubule diameter of 191.83 microm in the second group. We observed multilayer germinal epithelia with the most significant production of gametes and a seminiferous tubule diameter of 202.61 microm in the third group. The diameters of the seminiferous tubules of the forth and fifth groups were 193.38 microm and 191.84 microm, respectively. The obtained data revealed that the most intensive morphological diversification of the seminiferous epithelium in cats occurs at about 7-8 months of age. The diameters of seminiferous tubules were highest in the third group of cats, and the activity of spermatogenesis of this group, expressed as the number of sperm per 10 mm(2), was also the most distinctive. The spermatogenesis process was most evident in cats between 12 and 36 months of age, which was also when the sperm concentration was highest per estimated surface. 相似文献
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Correlations between the behavior of recreational horses,the physiological parameters and summer atmospheric conditions
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Iwona Janczarek Izabela Wilk Edyta Zalewska Krzysztof Bocian 《Animal Science Journal》2015,86(7):721-728
The aim of this paper was to select atmospheric factors and their values, which may disrupt the correct behavior and physiological condition of recreational horses. The studies were carried out from 1 July until 1 September on 16 Anglo‐Arabian geldings. Each day, from 09.00 to 10.00 hours, the horses worked under saddle. The riders and the authors gave a qualitative behavioral assessment for each horse. Mood and willingness to work were evaluated. The quantitative assessment was called ‘incorrect behavior of the horse while riding’ (IBHR). The percentage time of duration and the number of occurrences of the features while riding were calculated. Heart rate, body temperature and respiratory rate were taken at 08.00 hours (resting measurement) and at 10.05 hours (post‐exercise measurement). Air temperature, relative air humidity, wind speed and atmospheric pressure were measured at 08.00 and 10.00 hours. The results showed that adverse changes in the behavior of recreational horses can occur if the horse is ridden when the air temperature is above 26°C and when wind speeds exceed 5.5 m/s. Such conditions may cause a reduction in the mood and willingness to work in horses. Physiological parameters like heart rate and body temperature seem to be more sensitive indicators of the horse body reaction to the weather than behavioral reactions. 相似文献
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Marcin Wysokowski ?ukasz Klapiszewski Dariusz Moszyński Przemys?aw Bartczak Tomasz Szatkowski Izabela Majchrzak Katarzyna Siwińska-Stefańska Vasilii V. Bazhenov Teofil Jesionowski 《Marine drugs》2014,12(4):2245-2268
Novel, functional materials based on chitin of marine origin and lignin were prepared. The synthesized materials were subjected to physicochemical, dispersive-morphological and electrokinetic analysis. The results confirm the effectiveness of the proposed method of synthesis of functional chitin/lignin materials. Mechanism of chitin modification by lignin is based on formation of hydrogen bonds between chitin and lignin. Additionally, the chitin/lignin materials were studied from the perspective of waste water treatment. The synthetic method presented in this work shows an attractive and facile route for producing low-cost chitin/lignin biosorbents with high efficiency of nickel and cadmium adsorption (88.0% and 98.4%, respectively). The discovery of this facile method of synthesis of functional chitin/lignin materials will also have a significant impact on the problematic issue of the utilization of chitinous waste from the seafood industry, as well as lignin by-products from the pulp and paper industry. 相似文献
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Horses intended for leisure riding do not undergo any selection and most often retired sports horses or defective horses are chosen, as a low selling price determines their purchase by a leisure riding center. Unfortunately, horses bought at low prices usually have low utility value, are difficult to handle, require a special or individual approach and do not provide satisfaction in riding. However, neither modern horse breeding nor scientific research address the need to breed horses for leisure activities. There is no clear definition of a model leisure horse and criteria or information for its selection are not readily available in scientific publications. A wide spectrum of research methods may be used to evaluate various performance traits in horses intended for leisure activities. The fact that the population of recreational horses and their riders outnumber sporting horses should attract the special attention of scientific research. Their utility traits need to be determined with modern technology and methods in the same way they are for sporting horses. Such a system of evaluation would be very helpful for riders. 相似文献
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Zuzanna Witkowska Marita
winiarska Mariusz Korczyski Sebastian Opaliski Damian Konkol Izabela Michalak Agnieszka Saeid Magorzata Mironiuk Katarzyna Chojnacka 《Journal of animal physiology and animal nutrition》2019,103(2):485-492
New bio‐based dietary supplement with micronutrients for livestock was elaborated. The new preparation was tested on laying hens to determine the influence of new biological feed additives on the level of trace elements in egg content. The diet of laying hens (Hy‐Line Brown, 30 weeks of age) was supplemented with soya bean meal enriched with Cu(II), Zn(II), Fe(II) and Cr(III) by biosorption. A total of 150 laying hens were divided into five groups: one control and four experimental. In the control group, microelements were supplemented in the inorganic form, whereas in experimental groups, Cu, Zn, Fe and Cr were replaced with soya bean meal enriched with a given microelement ion. The feeding experiment was conducted for 12 weeks and was divided into three series. The results showed that adding the new feed additives to the diet of laying hens had an impact on microelement transfer to eggs, in particular with increased dosing. Eggs were biofortified with iron, zinc and copper and to a lesser extent with chromium. The microelements were accumulated primarily in the albumen because soy protein was the carrier of micronutrient ions in hens’ diet. Transfer of trace elements to eggs was not linearly dependent on the dosage of biologically bound microelements in the diet. 相似文献