Disposition Kinetics,Bioavailability and Renal Clearance of Cefepime in Calves

The pharmacokinetics of cefepime were studied following intravenous and intramuscular administration of 6.5 mg/kg in four female Friesian calves. Following single intravenous administration, the serum concentration-time curves of cefepime were best fitted using a two-compartment open model. The elimination half-life (t(1/2)beta) was 2.38+/-0.16 h, volume of distribution at steady state (Vdss) was 0.21 +/- 0.01 L/kg, and total body clearance (ClB) was 1.1 +/- 0.08 ml/min per kg. Following intramuscular administration, the drug was rapidly absorbed with an absorption half-life (t(1/2)ab) of 0.29+/-0.02 h; maximum serum concentration (Cmax) of 21.7 +/- 1.1 microg/ml was attained after (Tmax) 1.1 +/- 0.08 h; and the drug was eliminated with an elimination half-life (t(1/2)el) of 3.02 +/- 0.18 h. The systemic bioavailability (F) after intramuscular administration of cefepime in calves was 95.7% +/- 7.44%. The in vitro serum protein-binding tendency was 10.5-16.7%. Following administration by both routes, the drug was excreted in high concentrations in urine for 24 h post administration.     

Characterization of root rot disease of kiwifruit in the Black Sea region of Turkey

Forty-two kiwifruit orchards from Rize and Samsun provinces (Black Sea region, Turkey) exhibiting symptoms of root rot disease were examined between 2009 and 2010. Twenty-four fungal isolates recovered from affected roots were included in this study. Morphological characteristics of all isolates were recorded on potato dextrose agar, malt extract agar and Spezieller Nährstoffarmer agar. The effect of temperature on radial colony growth was also evaluated at 5, 10, 15, 20, 25, 30, and 35 °C. Histone H3 gene (HIS) was amplified with primers CYLH3F and CYLH3R and the amplified fragments were sequenced. The HIS phylogeny grouped all the isolates into six well-supported clades which were in agreement with phenotypical characteristics. Isolates were identified as “Cylindrocarpon” pauciseptatum, Cylindrocladiella parva, Ilyonectria liriodendri, I. torresensis, I. robusta and I. europaea, I. liriodendri being the most frequent species. All of them are reported for the first time on kiwifruit in Turkey, with the exception of I. liriodendri. Pathogenicity tests with selected isolates showed that 10 out of 11 isolates tested were able to induce typical root rot disease symptoms, affecting plant development and leading to the death of some plants. This study shows the high diversity of root rot pathogens found in kiwifruit trees in the Black Sea region of Turkey, being the first step towards a better understanding and management of the disease in local conditions.     

Dihydrooxadiazines: Octopaminergic system as a potential site of insecticidal action

Dihydrooxadiazines are structural analogs of octopamine and were compared with octopamine for their ability to compete with [³H]dihydroergocryptine ([³H]DHE) for binding sites on DHE-sensitive receptors, to stimulate adenylate cyclase activity in nervous system homogenates of Periplaneta americana L., and to modulate the action of the peptide proctolin on the oviducal muscles of Locusta migratoria L. [³H]DHE binding was inhibited by low concentrations (μM range) of octopamine, phentolamine, N-demethylchlordimeform (DCDM) and several dihydrooxadiazines. The tested dihydrooxadiazines acted as aminergic agonists in stimulating cyclic AMP production in cockroach nervous system homogenates and did not show additive effects with octopamine, whereas additivity was observed with 5-hydroxytryptamine. The relative potency of octopaminergic antagonists, including mianserin, cyproheptadine, phentolamine, and gramine, to block octopamine-mediated elevation of cyclic AMP production was similar to the rank-order potency of the same antagonists to inhibit dihydrooxadiazine-mediated elevation of cyclic AMP production. Octopamine, 2-(4-bromophenyl)-5,6-dihydro-4H-1,3,4-oxadiazine (4-Br-PDHO), and 8-Br-cyclic AMP caused increased phosphorylation of proteins that are phosphorylated by exogenously added cyclic AMP-dependent protein kinase. These results indicate that the dihydrooxadiazine-induced rise in cyclic AMP levels in homogenates of the cockroach nervous system results directly in activation of an endogenous cyclic AMP-dependent protein kinase. 4-Br-PDHO behaved similarly to octopamine in modulating the action of proctolin-induced contractions in locust oviducal muscles. These observations suggest that dihydrooxadiazines act as octopamine agonists and have an octopaminergic action in modulating the action of proctolin. Thus, it is proposed that dihydrooxadiazines exert at least part of their insecticidal and miticidal actions through interaction with the octopaminergic system.     

Metabolism, uptake and translocation of 14C-paraquat in resistant and susceptible biotypes of Crassocephalum crepidioides (Benth.) S. Moore

The metabolism, uptake and translocation of paraquat in resistant (R) and susceptible (S) biotypes of Crassocephalum crepidioides (Benth.) S. Moore (redflower ragleaf) at the 10-leaf stage was investigated. A study on the properties of leaf surface was carried out to examine the relationship between leaf surface characters and paraquat absorption. The extractable paraquat was not metabolized by the leaf tissue of either the resistant or susceptible biotypes. Differential metabolism, therefore, does not appear to play a role in the mechanism of resistance. Both biotypes did not show any significant difference in the amount of cuticle and trichome densities. Furthermore, both biotypes are identical in the structure of stomata, trichomes and epicuticular wax. The results of the leaf surface studies are in agreement with the findings of the uptake study. Both biotypes demonstrated no significant difference in absorption between the resistant and susceptible biotypes. However, 10% of the absorbed ¹⁴C-paraquat into the S biotype was translocated basipetally, but not in the R biotype. The results of this study suggest that in C. crepidioides , differential translocation may contribute to the mechanism of resistance at the 10-leaf stage.     

The utility of Trichoderma spp. isolates to control of Xylosandrus germanus Blandford (Coleoptera: Curculionidae: Scolytinae)

Journal of Plant Diseases and Protection - The invasive ambrosia beetle, Xylosandrus germanus Blandford (Coleoptera: Curculionidae: Scolytinae), is a serious pest of hazelnut in Turkey, which is...     

The influence of thermal stabilization stage on the molecular structure of polyacrylonitrile fibers prior to the carbonization stage

Structural transformation from a linear chain structure to crosslinked chain structure, occuring during the thermal stabilization stage of carbon fiber manufacture, was followed through the use of infrared spectroscopy, differential scanning calorimetry (DSC), elemental analysis and gel-fraction measurements. The results obtained from the analysis of IR spectroscopy showed the gradual and continuous loss of intensity of the nitrile (C≡N) vibration at 2242 cm⁻¹ closely associated with the cyclization reactions whereas the intensity loss of the methylene (CH₂) groups vibration around 2920–2939 cm⁻¹ has been attributed to the loss of hydrogen atoms as part of the dehydrogenation reactions. The dehydrogenation index, evaluated using the absorbance ratio of A₁₄₅₂/A₁₃₆₈, also indicated the gradual loss of hydrogens in agreement with decreasing hydrogen content with progressing stabilization process. IR spectroscopy also showed the emergence of new absorption bands attributed to the formation of crosslinked ladder-like structure in the 1590–1600 cm⁻¹ region. The amount of newly formed crosslinked structure was characterized using DSC conversion index, IR conversion index and gel-fraction measurements. The results are compared and discussed in detail.     

Effect of waxing and cellophane lining on chemical quality indices of citrus fruit

Effects of waxing of the fruit and of the use of cellophane for lining storage boxes on chemical quality indices (including ethanol and acetaldehyde) and flavour scores of Feutrell's Early mandarin during storage at room conditions were determined. Maximum changes during storage of waxed mandarins in film-lined boxes occurred in ethanol contents which were followed by acetaldehyde, total soluble solids (TSS)/acid ratio, TSS and acid contents. There were negative and highly significant linear relationships between flavour scores, and ethanol content and acetaldehyde content. The r² values indicated that over 90% of the variation in flavour scores could be explained by changes in ethanol and acetaldehyde contents whereas only 50% of the variation in flavour scores could be predicted by changes in total acid and TSS/acid ratios. There were comparatively greater increases in ethanol and acetaldehyde content, lesser increases in TSS and greater decreases in total solids of waxed mandarins during storage than those of unwaxed fruits stored in film lined boxes; the ratio of ethanol and acetaldehyde (E/A) of waxed mandarins was also higher. Decreases in citric acid content during storage correlated linearly to increases in the E/A ratio at the 1% level of significance indicating that citric acid metabolism was affected during storage of waxed mandarins at ordinary room conditions.     

Building shade affects light environment and urban greenery in high-density residential estates in Singapore

Urban green spaces are an important component of the urban ecosystem of cities as they provide a range of ecosystem services that contribute to sustainability and livability of urban areas. The extent to which such services are provided is influenced by limitations on biological processes that underpin such ecosystem services. A poorly understood limitation in the urban environment is the effects of shade created by buildings on the adequacy of photosynthetically active radiation (PAR) for plant growth. We examined the effects of building shade in high-density, high-rise residential estates in Singapore on the level and distribution of PAR, and how PAR might in turn be correlated with growth of plants in community green spaces nested within these estates. Our estimates showed that high-rise and high-density buildings reduced daily PAR by almost 50% when compared to fully exposed conditions. The reduced PAR levels were correlated with lower vegetative and reproductive growth of several species of shrubs, and increased slenderness of two tree species. The shade environment created by buildings was differentiated from shade under vegetation canopies by longer periods of high instantaneous PAR during a diurnal cycle. There was also evidence of higher red to far-red ratio in the spectral composition of PAR. We suggest that an understanding of the spatial and temporal characteristics of PAR is necessary for appropriate selection of plants, particularly to match daily PAR received on site to daily light integral requirements of plants for improved delivery of ecosystem services.     

Strawberry Shelf Life,Composition, and Enzymes Activity in Response to Edible Chitosan Coatings

A series of active biodegradable coatings based on chitosan, gelatin, starch, and sorbitol with or without monoterpenes (geraniol and thymol) were prepared and applied on fresh strawberry fruit as postharvest treatments. The coated fruits were inoculated with fungal spores of Botrytis cinerea and stored for 7 days at 4 °C. Decay incidence, weight loss, anthocyanins, total soluble solids (TSS), total soluble phenolics (TSP), polygalacturonase (PGase), pectin-lyase (PLase), antioxidant activity, guaiacol peroxidase (G-POD), polyphenol oxidase (PPO), and catalase (CAT) were elucidated in the fruits of experimental sets and they were compared with that of controls. The coatings showed a significant effect on the development of quality variables, with the additional effect of geraniol and thymol as a function of the polysaccharide matrix. The coatings inhibited decay incidence, reduced weight loss, delayed changes in the contents of anthocyanin, TSS, and total soluble phenolics; inhibited the increase in G-POD and PPO activities; and retarded the reduction in CAT activity. Compared to the controls, all of the coatings had positive effects on the inhibition of cell wall degrading enzymes (PGase and PLase) and among all the tested coatings, T5, chitosan (1%) + starch (1%) + sorbitol (0.5%) + tween (0.05%) + thymol (0.02%) was the best. This formulation showed also the highest antimicrobial activity and the greatest effect on other physiochemical parameters and it can be suggested to use it as a useful coating agent for extending the shelf-life and maintaining quality of strawberry fruit.     

Effects of root diffusates of some weeds associated with corn plantations on the hatchability of the corn cyst nematode,Heterodera zeae

Twenty two summer weeds belonging to fifteen plant families and twelve winter weeds from eight plant families were tested to compare the effects of their root diffusates with the corn root diffusates (CRD) of cv. Giza 2 on the hatchability of the corn cyst nematode (CCN),Heterodera zeae.All tested weed root diffusates (WRD) successfully stimulated the hatchability of the cysts, but less than CRD did except the root diffusates (RD) ofRumex dentatus which failed to stimulate the nematode cysts.