Quest for the piroplasms in camels: identification of Theileria equi and Babesia caballi in Jordanian dromedaries by PCR

DNA of two species of piroplasmids was detected in dromedaries during a survey of blood protozoans in Jordan between 2007 and 2009. Ten clinically healthy camels (10%) originating from three Jordanian districts were found, using a PCR assay, to harbor Theileria or Babesia species in their blood and no mix infection was determined. Analysis of the partial 18S rRNA gene sequences of these parasites allowed their unambiguous identification as equine piroplasmids Babesia caballi (n=6) and Theileria equi (n=4). In case of latter species, a novel genotype was found in horses. This first molecular-based species determination of piroplasmids from camels further contributes to the growing evidence of low host specificity of piroplasmids.     

Estimating direct healthcare costs attributable to laboratory‐confirmed Lyme disease in Ontario,Canada: A population‐based matched cohort study using health administrative data

The objective of this study was to determine healthcare costs attributable to laboratory‐confirmed Lyme disease (LD) from the healthcare payer perspective in Ontario, Canada. A cost‐of‐illness study was conducted for incident LD subjects from 1 January 2006 through 31 December 2013 ascertained from provincial laboratory and reportable disease databases, linked to health administrative data. All LD subjects included were laboratory‐confirmed, according to provincial case definitions. Incident LD subjects were propensity‐score matched to uninfected subjects on age, sex, comorbidities and urban/rural status. We used phase‐of‐care methods to calculate attributable costs for two phases of illness: initial care (≤30 days following “index date”) and continuing care (>30 days after index date to the end of the follow‐up period). A total of 663 incident, confirmed LD subjects were identified from 2006 through 2013. Mean age was 44.2 ± 20.1 years; 339 (51.1%) were female; and 31 (4.7%) were hospitalized ≤30 days after index date. Six hundred fifty‐eight (99.2%) LD subjects were matched to uninfected subjects; mean follow‐up time was 3.3 years. Mean attributable costs per case during the initial care phase and continuing care were $277 (95% CI: $197, $357) and ?$5 (?$27, $17), respectively. Attributable costs per LD subject aged 5–14 years were $440 ($132, $747), greater than the costs observed for other age strata. Expected 1‐year attributable costs were $832, given continuing care costs were negligible. Limitations to our study include estimating costs using a cohort of only laboratory‐confirmed LD cases, introducing selection bias for diagnosed and treated patients who may have a lower risk of developing sequelae. In conclusion, the initial care phase of LD is associated with increased healthcare costs, but without significant costs attributable to LD infection after 30 days. Estimates of costs attributable to LD are important for healthcare resource prioritization and the evaluation of novel interventions.     

Dietary supplementation for Santa Inês hair ewes on pasture at pre- and postpartum periods: dry matter intake,digestibility, milk production,and mineral metabolism

The aim of this study was to evaluate the impact of different levels of dietary supplementation and reproductive stages on dry matter intake, digestibility, milk production, and mineral metabolism in Santa Inês hair ewes. Two dietary supplement levels of 0.5 and 1.5%, based on body weight, were used. A total of 12 hair ewes (six subjected to 0.5 and six subjected to 1.5% of concentrate supplementation based on body weight—BW) of the Santa Inês breed were evaluated in a completely randomized design with fixed effects of supplementation level, period, and its interactions. Dry matter intake, digestibility, milk production, and mineral metabolism (calcium (Ca), phosphorus (P), magnesium (Mg), alkaline phosphatase (ALP), type I insulin-like growth factor (IGF-I), parathyroid hormone (PTH), and osteocalcin (OC)) were assessed. Dry matter digestibility was affected by the supplementation level (during both pregnancy and lactation), with higher values in ewes fed at a level of 1.5% of BW. A significant interaction between treatment × reproductive stages was found for the Mg concentration. A period effect (P?<?0.05) on serum concentrations of P, Ca/P, Mg, and IGF-I was observed. Serum P concentrations were influenced (P?<?0.05) by treatments and reproductive stages. There were significant differences in the Ca/P ratio among the reproductive stages. The enzymatic activity of ALP and serum IGFI differed among reproductive stages. Ewes supplemented at a level of 1.5% of BW produced 18.5% more milk than ewes supplemented at a level of 0.5% of BW. The use of 0.5% of body weight in concentrate supplementation is recommended for the reduction of production costs, without having an effect on the mineral metabolism of Santa Inês hair ewes.

     

Evaluation of Four Commercial Grower Diets for Production of Largemouth Bass

We compared performance (growth, survival, condition, and health indices) of largemouth bass, Micropterus salmoides, fingerlings (10.3 g [SE ± 0.5 g] and 92 mm [SE ± 1 mm] total length) fed one of four commercial grower feeds: Purina Aquamax Grower 400 (PAG; PMI Nutrition International LLC), Bio-Oregon BioDiet Grower (BDG; Bio-Oregon), Silver Cup Steelhead (SCS; Silver Cup), and Richloam Bass Fry Diet 12 (RBF; Silver Cup). Sixteen 833-L circular tanks, connected in parallel to a recirculation system, were initially stocked with 2.25 kg of fish (219 fish each, 0.32 fish/L) with four replicates per feed treatment. Water temperature was 25°C, and photoperiod was 15 h light:9 h dark. Feed sizes (2.4–3.0 mm) were comparable, and protein compositions ranged from 49% to 61%. Fish were fed to satiation multiple times per day (trial duration = 100 days). Overall, performance was best for fish fed RBF, followed by PAG and BDG, and then SCS. The SCS feed resulted in significantly lower growth and survival (P ≤ 0.05). The PAG and BDG feeds resulted in significantly lower growth than the RBF feed (P ≤ 0.05); however, survival did not differ (P > 0.05). Performance measures were positively correlated with protein, and protein to energy ratio and negatively correlated with fat and carbohydrate compositions. The four commercial grower feeds resulted in highly varied growth, survival, and health indices, and this research should facilitate feed choice for various largemouth bass production strategies as well as increase contribution of stocked fish.     

Temporary immersion and stationary bioreactors for mass propagation of true-to-type highbush,half-high,and hybrid blueberries (Vaccinium spp.)

True-to-type propagules in half-high, highbush, and hybrid blueberries (Vaccinium L. spp.) were produced using stationary (SB) and temporary immersion bioreactor (TIB) systems containing a liquid medium. Multiple shoots were produced in vitro from nodal segments of blueberry cultivars ‘St. Cloud’ and ‘Polaris’, and of six blueberry hybrids obtained from crossing between half-high/highbush and lowbush blueberries. Shoot proliferation was best in a liquid medium containing 4.6 µM zeatin in both TIB and SB systems, but the performance was genotype dependent. Shoot proliferation was better in hybrids than in cultivars. Although SB produced longer shoots with more leaves per shoot in most of the genotypes, TIB-derived shoots were more vigorous and rooted better under ex vitro condition. Liquid culture-derived elongated shoots were rooted ex vitro by treating with indole-3-butyric acid (39.4 mM) before planting on a 3 peat:2 perlite (v/v) medium. Micropropagules were acclimatized and maintained in a greenhouse with 80?90% survival rate of rooted plantlets. Expressed sequence tag (EST)-polymerase chain reaction (PCR) and EST- and genomic-simple sequence repeat (SSR) marker assay formed a homogenous monomorphic banding pattern in the in vitro-derived and donor control plants proving the clonal fidelity of liquid-culture-derived micropropagated plants.     

Comparative Assessment of Nitrogen Concentration Effect on Microalgal Growth and Biochemical Characteristics of Two Chlorella Strains Cultivated in Digestate

Microalgae have been recently recognized as a promising alternative for the effective treatment of anaerobic digestion effluents. However, to date, a widely applied microalgae-based process is still absent, due to several constraints mainly attributed to high ammonia concentrations and turbidity, both hindering microalgal growth. Within this scope, the purpose of the present study was to investigate the performance of two Chlorella strains, SAG 211-11b and a local Algerian isolate, under different nitrogen levels, upon ammonia stripping. The experiments were performed on cylindrical photobioreactors under controlled pH (7.8 ± 0.2) and temperature (25 ± 2 °C). Cultures were monitored for biomass production and substrate consumption. After sampling at the beginning of the stationary phase of growth (12th day) and after the maturation of the cells (24th day), an analysis of the produced biomass was conducted, in terms of its biochemical components. The local isolate grew better than C. vulgaris 211-11b, resulting in 1.43 mg L⁻¹ biomass compared to 1.02 mg L⁻¹ under 25 mg NH₄-N L⁻¹, while organic carbon and nutrient consumption varied between the two strains and different conditions. Concerning biomass quality, a high initial NH₄-N concentration led to high protein content, while low nitrogen levels favored fatty acid (FA) accumulation, though the production of pigments was inhibited. In particular, the protein content of the final biomass was determined close to 45% of the dry weight in all experimental scenarios with adequate nitrogen, while proteins decreased, and the fatty acids approached 20% in the case of the local isolate grown on the substrate with the lowest initial ammonium nitrogen (25 mg NH₄-N L⁻¹). The novelty of the present work lies in the comparison of a microalga with industrial applications against a local isolate of the same species, which may prove to be even more robust and profitable.     

Mapping quantitative trait loci (QTLs) associated with dough quality in a soft × hard bread wheat progeny

Bread wheat (Triticum aestivum L.) quality is a key trait for baking industry exigencies and broad consumer preferences. The main goal of this study was to undertake quantitative trait loci (QTL) analyses for bread wheat quality in a set of 79 recombinant inbred lines (RILs) derived from a soft × hard bread wheat cross. Field trials were conducted over two years, utilizing a randomized complete block design. Dough quality was evaluated by sedimentation test, mixograph and alveograph analysis. Protein content was measured by near-infrared reflectance analysis and grain hardness was determined by the single kernel characterization system (SKCS).     

Variation in spawning responses, egg and larvae productions from induced rohu (Labeo rohita) during pre-monsoon and monsoon seasons: Relationship with hormonal changes and oocyte responsiveness during final maturation

Early induced spawning in captive rohu (Labeo rohita) often encounters with reduced spawning performances and devaluation of final product. The present study attempted to gain insight into the problems associated with poor performance of rohu during pre-monsoon spawning. A combination of sGnRHa and domperidone was used to induce final oocyte maturation (FOM) and ovulation in rohu during early (pre-monsoon, PM) and normal (monsoon, MN) spawning. The spawning performance parameters such as, spawning response, production and quality of egg and larvae showed significantly lower values (p < 0.05) in PM, when compared with MN spawning. The egg and spawn productions were recorded as 2.6 ± 0.05 and 2.41 ± 0.05 during the MN season, which were reduced by almost 50% in the PM season. Moreover the quality of egg and hatchling was devaluated significantly (p < 0.05) and exhibited higher percentage of mortality and abnormality in PM than those recorded in the MN season. The plasma concentration of carp gonadotropin (cGtH), 17β-estradiol (E) and 17α20β-dihydroxy-4-pregnen-3-one (DP) in relation to progress of FOM and ovulation at different seasons exhibited marked variation in hormonal profiles particularly in E and DP of PM fish. Higher initial plasma E (3.8 ± 0.3) and a distinct E peak clearly indicated the lack of transition from vitellogenic to post-vitellogenic stages that prevailed in PM rohu. Delayed DP and cGtH surge during FOM and ovulation resulted in longer latency period in spite of higher water temperature (31.5 °C) that prevailed during the PM period. In-vitro study on oocyte maturational competence (OMC) clearly depicted the lack of maturational competence in ovarian follicles during PM than MN in rohu. However priming the fish with purified carp gonadotropin (PCG) enhanced the acquisition of OMC in PM rohu in such an extent, that no marked seasonal differences (p > 0.05) in OMC were remained further, when compared with MN follicles. The PCG mediated acquisition of maturational competence was found to be dependent fully on new mRNA and protein synthesis in PM fish. The present study clearly demonstrated that the oocytes' unresponsiveness to hormonal induction was mainly responsible for reduced spawning performance in PM rohu, which could be ameliorated through PCG priming to achieve better spawning response in rohu during the pre-monsoon period. Thereby, the rohu fry production could be initiated successfully as early as May, allowing public and private hatcheries to produce larger age-0 rohu fingerlings ensuring reliable steady source of stocking materials for grow-out system earlier in the season.