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991.
F. Doug Raymond Mark M. Alley David J. Parrish Wade E. Thomason 《Journal of plant nutrition》2013,36(3):395-409
ABSTRACT Corn (Zea mays L.) production recommendations should be periodically evaluated to ensure that production practices remain in step with genetic improvements. Since most of the recent increases in corn grain yield are due to planting at higher densities and not to increased per-plant yield, this study was undertaken to measure the effects of plant density and hybrid on corn forage and grain yield and on nutrient uptake. Plant density (4.9, 6.2, 7.4, and 8.6 seeds m?2) and hybrid relative maturity (RM) [early (108 day RM); medium (114 day RM); and late (118 day RM)] combinations were evaluated over five site-years under irrigated and non-irrigated conditions. The interaction of hybrid with plant density was never significant for grain, stem, or leaf biomass. The latest RM hybrid out-yielded the medium and early hybrids by 550 and 1864 kg ha?1, respectively. Grain yield was highest at 8.6 plants m?2. Total stem yield was also greatest at the highest plant density but by only 340 kg ha?1 more than at 7.4 seeds m?2. Based on grain yield response over sites, the estimated optimum density was 7.6 seeds m?2, which is 0.7 seeds m?2 higher than the current recommendation at this average yield level (11.5 Mg ha?1). Grain nitrogen (N), phosphorus (P), and potassium (K) uptakes were highest for the medium RM hybrid. Nutrient uptake levels varied by planting density, with the lowest levels observed at the lowest and highest plant densities. At 4.9 seeds m?2, the reduced uptake is explained by lower biomass yields. At the 8.6 seeds m?2 rate, N and K levels may have been lower due to dilution. 相似文献
992.
993.
994.
Snégaroff J Branlard G Bouchez-Mahiout I Laudet B Tylichova M Chardot T Pecquet C Choudat D Raison-Peyron N Vigan M Kerre S Laurière M 《Journal of agricultural and food chemistry》2007,55(24):9837-9845
Two genes of wheat low-molecular-weight glutenin subunits (LMW-GS), B16 and P73, were cloned and expressed in E. coli. They were homologous to proteins encoded respectively at Glu-B3 and Glu-D3 loci. The N-terminal and C-terminal halves of B16 (NB16 and B16C) and the two chimeras combining the halves of the two genes (B16-P73 and P73- B16) were also expressed. All these constructs were compared for their reactivity with IgE from 24 patients suffering from different forms of wheat allergies. The results confirmed that LMW-GSs bound IgE in all adult allergies tested. Strong differences in reactivity between all the constructs were observed. They were disease-dependent. In wheat-dependent exercise-induced anaphylaxis (WDEIA), the reactivity of the constructs depended partly on common epitopes with omega-5 gliadins but also on differences in molecule conformation. The presence of NB16 in the constructs greatly influenced their IgE reactivity. 相似文献
995.
Tóth F Frank N Elliott SB Geor RJ Boston RC 《American journal of veterinary research》2008,69(1):82-88
OBJECTIVE: To evaluate the effects of endotoxin administered IV on glucose and insulin dynamics in horses. ANIMALS: 16 healthy adult mares. PROCEDURES: Each week of a 2-week randomized crossover study, each horse received an IV injection (duration, 30 minutes) of Escherichia coli O55:B5 lipopolysaccharide (LPS) in 60 mL of sterile saline (0.9% NaCl) solution (20 ng/kg) or sterile saline solution alone (control treatment). Frequently sampled IV glucose tolerance test procedures were performed at 24 hours before (baseline) and 24 and 48 hours after injection; glucose and insulin dynamics were assessed via minimal model analysis. RESULTS: 13 of 16 horses had a clinical response to LPS, which was characterized by mild colic and leukopenia. Before treatment, mean +/- SD insulin sensitivity was 2.9 +/- 1.9 x 10(4) L x min(1) x mU(1); this significantly decreased to 0.9 +/- 0.9 x 10(4) L x min(1) x mU(1) 24 hours after treatment (69% reduction) and was 1.5 +/- 0.9 x 10(4) L x min(1) x mU(1) 48 hours after treatment. At baseline, mean +/- SD acute insulin response to glucose was 520 +/- 196 mU x min x L(1); this significantly increased to 938 +/- 620 mU x min x L(1) (80% increase) and 755 +/- 400 mU x min x L(1) (45% increase) at 24 and 48 hours after LPS treatment, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Compared with baseline values, insulin sensitivity was decreased for 24 hours after IV injection of LPS, and affected horses had a compensatory pancreatic response. These disturbances in glucose and insulin dynamics may contribute to development of laminitis in horses. 相似文献
996.
Development of two real-time PCR assays for the detection of Mycoplasma hyopneumoniae in clinical samples 总被引:2,自引:0,他引:2
Dubosson CR Conzelmann C Miserez R Boerlin P Frey J Zimmermann W Häni H Kuhnert P 《Veterinary microbiology》2004,102(1-2):55-65
In order to improve the diagnosis of enzootic pneumonia (EP) in pigs two real-time polymerase chain reaction (rtPCR) assays for the detection of Mycoplasma hyopneumoniae in bronchial swabs from lung necropsies were established and validated in parallel. As a gold standard, the current "mosaic diagnosis" was taken, including epidemiological tracing, clinical signs, macro- and histopathological lesions of the lungs and immunofluorescence. One rtPCR is targeting a repeated DNA element of the M. hyopneumoniae genome (REP assay), the other a putative ABC transporter gene (ABC assay). Both assays were shown to be specific for M. hyopneumoniae and did not cross react with other bacteria and mollicutes from pig. With material from pigs of defined EP-negative farms the two assays showed to be 100% specific. When testing lungs from pig farms with EP, the REP assay detected 50% and the ABC assay 90% of the farms as positive. Both tests together detected all positive farms. Within a positive herd the two assays tested similarly with on average over 90% of the lung samples analysed from a single farm showing positive scores. A series of samples with suspicion of EP and samples from pigs with diseases other than respiratory taken from current routine diagnostic was assayed. None of the assays showed false positive results. The sensitivities in this sample group were 50% for the REP and 70% for the ABC assays and for both assays together 85%. The two assays run in parallel are therefore a valuable tool for the improvement of the current diagnosis of EP. 相似文献
997.
Nachreiner RF Refsal KR Graham PA Bowman MM 《Journal of the American Veterinary Medical Association》2002,220(4):466-471
OBJECTIVE: To determine prevalence of thyroid hormone autoantibodies (THAA) in serum of dogs with clinical signs of hypothyroidism. DESIGN: Cohort study. SAMPLE POPULATION: 287,948 serum samples from dogs with clinical signs consistent with hypothyroidism. PROCEDURE: Serum THAA were detected by use of a radiometric assay. Correlation and chi2 analyses were used to determine whether prevalence varied with breed, age, sex, or body weight. Only breeds for which > or = 50 samples had been submitted were used for analysis of breed prevalence. RESULTS: Thyroid hormone autoantibodies were detected in 18,135 (6.3%) samples. The 10 breeds with the highest prevalence of THAA were the Pointer, English Setter, English Pointer, Skye Terrier, German Wirehaired Pointer, Old English Sheepdog, Boxer, Maltese, Kuvasz, and Petit Basset Griffon Vendeen. Prevalence was significantly correlated with body weight and was highest in dogs between 2 and 4 years old. Females were significantly more likely to have THAA than were males. CONCLUSIONS AND CLINICAL RELEVANCE: Thyroid hormone autoantibodies may falsely increase measured triiodothyronine (T3) and thyroxine (T4) concentrations in dogs; results suggest that T3 concentration may be falsely increased in approximately 57 of 1,000 dogs with hypothyroidism and that T4 concentration may be falsely increased in approximately 17 of 1,000 dogs with hypothyroidism. Results also suggested that dogs of certain breeds were significantly more or less likely to have THAA than were dogs in general. 相似文献
998.
A catastrophic extinction occurred at the end of the Permian Period. However, baseline extinction rates appear to have been elevated even before the final catastrophe, suggesting sustained environmental degradation. For terrestrial vertebrates during the Late Permian, the combination of a drop in atmospheric oxygen plus climate warming would have induced hypoxic stress and consequently compressed altitudinal ranges to near sea level. Our simulations suggest that the magnitude of altitudinal compression would have forced extinctions by reducing habitat diversity, fragmenting and isolating populations, and inducing a species-area effect. It also might have delayed ecosystem recovery after the mass extinction. 相似文献
999.
M. S. Reed I. Fazey L. C. Stringer C. M. Raymond M. Akhtar‐Schuster G. Begni H. Bigas S. Brehm J. Briggs R. Bryce S. Buckmaster R. Chanda J. Davies E. Diez W. Essahli A. Evely N. Geeson I. Hartmann J. Holden K. Hubacek A. A. R. Ioris B. Kruger P. Laureano J. Phillipson C. Prell C. H. Quinn A. D. Reeves M. Seely R. Thomas M. J. van der Werff Ten Bosch P. Vergunst L. Wagner 《Land Degradation \u0026amp; Development》2013,24(4):307-322
It is increasingly recognised that land degradation monitoring and assessment can benefit from incorporating multiple sources of knowledge, using a variety of methods at different scales, including the perspectives of researchers, land managers and other stakeholders. However, the knowledge and methods required to achieve this are often dispersed across individuals and organisations at different levels and locations. Appropriate knowledge management mechanisms are therefore required to more efficiently harness these different sources of knowledge and facilitate their broader dissemination and application. This paper examines what knowledge is, how it is generated and explores how it may be stored, transferred and exchanged between knowledge producers and users before it is applied to monitor and assess land degradation at the local scale. It suggests that knowledge management can also benefit from the development of mechanisms that promote changes in understanding and efficient means of accessing and/or brokering knowledge. Broadly, these processes for knowledge management can (i) help identify and share good practices and build capacity for land degradation monitoring at different scales and in different contexts and (ii) create knowledge networks to share lessons learned and monitoring data among and between different stakeholders, scales and locations. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献