全文获取类型
收费全文 | 819篇 |
免费 | 31篇 |
专业分类
林业 | 102篇 |
农学 | 25篇 |
基础科学 | 5篇 |
247篇 | |
综合类 | 39篇 |
农作物 | 80篇 |
水产渔业 | 53篇 |
畜牧兽医 | 216篇 |
园艺 | 19篇 |
植物保护 | 64篇 |
出版年
2024年 | 3篇 |
2023年 | 11篇 |
2022年 | 10篇 |
2021年 | 24篇 |
2020年 | 25篇 |
2019年 | 39篇 |
2018年 | 31篇 |
2017年 | 23篇 |
2016年 | 32篇 |
2015年 | 18篇 |
2014年 | 32篇 |
2013年 | 40篇 |
2012年 | 66篇 |
2011年 | 75篇 |
2010年 | 49篇 |
2009年 | 30篇 |
2008年 | 53篇 |
2007年 | 57篇 |
2006年 | 39篇 |
2005年 | 39篇 |
2004年 | 35篇 |
2003年 | 33篇 |
2002年 | 29篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1999年 | 6篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1972年 | 3篇 |
1971年 | 1篇 |
排序方式: 共有850条查询结果,搜索用时 0 毫秒
151.
Romero-González R Alarcón-Flores MI Martínez Vidal JL Garrido Frenich A 《Journal of agricultural and food chemistry》2012,60(21):5324-5329
A new method has been developed and validated for the simultaneous determination of four biogenic (putrescine, cadaverine, histamine, and tyramine) and three volatile amines (trimethylamine, triethylamine, and tripropylamine) in anchovy. Separation and determination of the selected compounds were carried out by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), using an electrospray ionization source (ESI) in positive ion mode. Chromatographic separation was carried out using an aqueous solution of formic acid (0.1%) and methanol as mobile phase in gradient mode. The method was validated, and mean recoveries were evaluated at three concentration levels (75, 150, and 250 μg/kg), ranging from 70 to 110% at the three levels assayed. Intra- and interday precision, expressed as relative standard deviation (RSD), were lower than 15% and 20%, respectively. Limits of quantitation (LOQs) were 25 μg/kg for all cases, except for that of TMA, which was set at 60 μg/kg. The developed procedure was applied to determine the target compounds in anchovy samples stored during 7 days at 4 °C, observing the increasing in the concentration of these compounds at longer storage time. 相似文献
152.
Giner E Andújar I Recio MC Ríos JL Cerdá-Nicolás JM Giner RM 《Journal of agricultural and food chemistry》2011,59(24):12882-12892
Oleuropein, the major secoiridoid in olive tree leaves, possesses a wide range of health promoting properties. It has recently been shown to exhibit anti-inflammatory activity. We have evaluated the effect of oleuropein on dextran sulfate sodium (DSS)-induced experimental colitis in mice in order to provide insight into its mechanisms of action. Oral administration of oleuropein notably attenuated the extent and severity of acute colitis while reducing neutrophil infiltration; production of NO, IL-1β, IL-6, and TNF-α; expression of iNOS, COX-2, and MMP-9; and the translocation of the NF-κB p65 subunit to the nucleus in colon tissue. In LPS-stimulated peritoneal macrophages, the oleuropein metabolite, hydroxytyrosol, was shown to inhibit NO production, iNOS expression, NF-κB p65 subunit translocation, mRNA expression, and the release of IL-1β, IL-6, and TNF-α. These results suggest that the effect of oleuropein on DSS-induced colitis is associated with a decrease in the production of interleukins and expression of proteins, principally through reduction of NF-κB activation. 相似文献
153.
Apple (Malus × domestica Borkh.) fruit intended for long-term storage are frequently harvested commercially before becoming fully ripe, often resulting in poor aroma development. Since postharvest calcium dips have proved effective for the enhancement of flavor-related volatile esters after cold storage of apples, this study was undertaken in order to assess whether preharvest calcium sprays (7 weekly applications at 1.6%, w/v, 81-123 days after full bloom) could also aid in improving this important attribute at harvest. This procedure significantly increased calcium content in treated fruit. The emission of aroma-related volatile esters by untreated and calcium-treated 'Fuji' apples was then monitored during maturation and ripening over two months prior to commercial harvest. Results indicate that most of the compounds contributing to overall flavor in ripe fruit were enhanced in response to preharvest calcium applications, suggesting that this procedure may be suitable for the improvement of fruit aroma at harvest. The emission of acetate esters was particularly favored, consistent with higher acetaldehyde contents in treated fruit. These effects arose apparently from increased pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) activities, possibly leading to a better supply of alcohols and acyl CoAs for ester biosynthesis. 相似文献
154.
Optimization of conditions for anthocyanin hydrolysis from red wine was investigated using response surface methodology. The aglycon forms of the anthocyanins were quantified by high-performance liquid chromatography with diode array detection. The combined effects of three independent variables, HCl amount, heating temperature, and hydrolysis time, were studied using a 2(3) full-factorial central composite design. Anthocyanin hydrolysis yield depended mainly on the heating temperature and time of hydrolysis. HCl amount was the factor that least influenced the hydrolysis of anthocyanins. From experimental results, the maximum yield of anthocyanidins was reached with 9.8 mL of HCl (32% v/v), a heating temperature of 166.2 °C, and a hydrolysis time of 46.6 min. Five anthocyanidins, namely, delphinidin, cyanidin, petunidin, peonidin, and malvidin, were quantified in red wine. The reliability of the method was confirmed by recovery experiments, performed under optimal conditions. Recoveries indicated that anthocyanidins resisted the hydrolysis conditions. 相似文献
155.
Daphne Isabel Jost Rainer Georg Joergensen Albert Sundrum 《Biology and Fertility of Soils》2013,49(1):61-70
A study was carried out to investigate the effects of different diets for heifers, low- and high-yielding cows on the microbial composition of their faeces and subsequently the impacts of these faeces on CO2 and N2O emissions, N mineralisation and plant N uptake. A diet low in N and high in acid detergent fibre offered to heifers resulted in faeces dominated by fungi. These faeces were characterised by a low content in microbial biomass C and N and a high ergosterol concentration in comparison to the faeces of high-yielding cows. Added to soil, faeces of heifers led to lower emission and stronger N immobilisation during a 14-day incubation in comparison to the faeces of high-yielding cows. Total N2O emission was significantly (P?<?0.05) correlated with faecal microbial biomass N. Rye grass yield and N uptake were lowest in the soil supplemented with faeces from heifers in a 62-day pot experiment. Plant N uptake was influenced by the faecal microbial biomass C/N ratio and the fungal C to bacterial C ratio. In conclusion, the faecal microbial biomass was affected to a high degree by the feeding regime and faecal microbial characteristics revealed higher impacts on plant N uptake than soil microbial properties. 相似文献
156.
157.
The root lesion nematode Pratylenchus penetrans parasitizes a wide range of economically important crops, including potato (Solanum tuberosum). Damage by P. penetrans impacts not only the potato yield but can also reduce the tuber quality. Detailed information on tuber infection by P. penetrans is scarce for most cultivars and molecular detection of nematodes from infected tubers is needed. The objective of this study was to assess tuber symptomatology due to P. penetrans infection in 10 potato cultivars and to provide an accurate molecular methodology for nematode detection using tuber peels. Sprouts of certified potato seed from cultivars Agata, Agria, Camel, Désirée, Dirosso, Kennebec, Laura, Picasso, Royata, and Stemster were planted in 2 L pots, and soil was inoculated with 4 P. penetrans/g of soil. Sixty days after inoculation, tubers were harvested, inspected for lesions, and the number of nematodes/g of potato peel assessed. Observations of tubers with symptoms showed the presence of P. penetrans in superficial layers of peels around the lenticels and injured necrotic tissue. Different nematode stages were detected in tubers of all inoculated cultivars, varying from 4 to 46 nematodes/g of potato peel. Species-specific primers showed suitable sensitivity and reproducibility for the detection of P. penetrans in tuber potato peel samples. The molecular detection of P. penetrans directly from tuber peels can facilitate routine nematode inspections of potato seed tubers or cull potatoes for nematode detection, and prevent further dissemination of this species. 相似文献
158.
Alfaro-Fernández Ana Hernández-Llopis Desamparados Font María Isabel 《European journal of plant pathology / European Foundation for Plant Pathology》2022,164(1):147-147
European Journal of Plant Pathology - 相似文献
159.
M. Pilar Gómez-Serranillos Sara Martín Teresa Ortega Olga M. Palomino Marín Prodanov Visitación Vacas Teresa Hernández Isabel Estrella M. Emilia Carretero 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(4):238-243
Phenolic composition of wine depends not only on the grape variety from which it is made, but on some external factors such
as winemaking technology. Red wine possesses the most antioxidant effect because of its high polyphenolic content. The aim
of this work is to study for the first time, the neuroprotective activity of four monovarietal Spanish red wines (Merlot (ME),
Tempranillo (T), Garnacha (G) and Cabernet-Sauvignon (CS)) through its antioxidant ability, and to relate this neuroprotection
to its polyphenolic composition, if possible. The wine effect on neuroprotection was studied through its effect as free radical
scavenger against FeSO4, H2O2 and FeSO4 + H2O2. Effect on cell survival was determined by 3(4,5-dimethyltiazol-2-il)-2,5-diphenyltetrazolium reduction assay (MTT) and lactate
dehydrogenase (LDH) release assay on astrocytes cultures. Results showed that most of the studied wine varieties induced neuroprotection
through their antioxidant ability in astrocytes, Merlot being the most active; this variety is especially rich in phenolic
compounds, mainly catechins and oligomeric proanthocyanidins. Our results show that red wine exerts a protection against oxidative
stress generated by different toxic agents and that the observed neuroprotective activity is related to their polyphenolic
content. 相似文献
160.
Isabel Bondia-Pons Anna-Marja Aura Satu Vuorela Marjukka Kolehmainen Hannu Mykkänen Kaisa Poutanen 《Journal of Cereal Science》2009
Dietary intake of whole-grain foods is associated with a decreased risk of chronic diseases such as diabetes, obesity and heart disease. In addition to dietary fibre, various phytochemicals have been suggested to contribute to the health effects of whole grain products. This review focuses on phenolic compounds in rye (Secale cereale L.), which is one of the major bread grains in Europe. Data on phenolic concentrations in rye grain and foods, their bioavailability to tissues and effects in vivo, and their potential contributions to health are presented. Phenolic compounds in rye, such as phenolic acids, alkylresorcinols and lignans, are concentrated in the outer layers of the grain. Phenolic acids are the major phenolic compounds in whole grain rye (103–300 mg/100 g grain), ferulic acid being the most abundant. Rye lignans are present at concentrations of 2 mg/100 g grain and had been shown to be converted by the intestinal microflora to the mammalian lignans enterodiol and enterolactone in human intervention studies. Alkylresorcinols (36–320 mg/100 g grain), which have been found to be incorporated into human erythrocyte membranes, are of particular interest due to their potential use as biomarkers of the intake of rye and wheat. 相似文献