Physiological and metabolic properties of a digestion-resistant maltodextrin, classified as type 3 retrograded resistant starch

There is a growing interest in highly fermentable dietary fibers having the potential to reduce risks of disease through the production of short-chain fatty acids (SCFA). Recently a digestion-resistant retrograded maltodextrin (RRM), classified as type 3 resistant starch was developed. Systematic work to determine its molecular and physiological properties was carried out to determine (1) the fraction resistant to digestion in vitro and in vivo, (2) its postconsumption effect on blood glucose in healthy volunteers, and (3) its in vitro fermentation pattern, at different ages, by use of pooled fresh human fecal inoculum. RESULTS: The digestion resistant fraction obtained in vivo from ileostomy patients (59.4%) is similar to that obtained by the AOAC method for measuring retrograded resistant starch (59.7%). The relative glycemic response after consumption of 50 g of RRM was 58.5% compared to glucose set as 100%. When exposed to colonic microbiota, in vitro obtained indigestible fractions behave similarly to those obtained in vivo in ileostomy patients. Fermentation of RRM and production of butyric acid is negligible during the first months of life but develops subsequently during weaning. In adults, RRM fermentation results in a high yield of SCFA, with butyrate representing 21-31 mol % of total SCFA. The high yield of SCFA during colonic fermentation, observed from weaning age on, as well as the potential to help reduce glycemic load may be of benefit to a number of health-related functions in the host. Further study on clear clinical end points is warranted.     

Comparison of Docosahexaenoic Acid (22:6n-3) Levels in Various Artemis Strains during Enrichment and Subsequent Starvation

Abstract.— The aim of this study was to compare the levels of docosahexaenoic acid (DHA, 22:6n-3) in three different bisexual and one parthenogenetic strains of Artemia after enrichment. Freshly-hatched nauplii from A. franciscana (Great Salt Lake, USA), A. sinica (Yimeng, P. R. China), A. persimilis (Argentina), and A. parthenogenetica (Tanggu, P. R. China) were enriched with a purified lipid emulsion containing 95% DHA ethyl esters (% total fatty acids) and subsequently starved. All strains had very low initial DHA levels (< 0.3 mg/g dry weight). Initial eicosapentaenoic acid, 20:5n-3 (EPA) levels were high in A. parthenogenetica (18.2 mg/g dry weight) as compared to those in the other strains (4.6-8.5 mg/g dry weight). After 24-h enrichment, A. sinica contained the highest DHA level (37.0 mg/g dry weight) as well as the highest DHA/ EPA ratio (3.7). The lowest DHA enrichment levels were found in A. franciscana and A. parthenogenetica (26.3 and 22.7 mg/g dry weight, respectively). During the subsequent 24-h starvation period, the contents of DHA decreased rapidly in all strains, whereas EPA levels remained relatively stable. This indicates the high catabolism of DHA for energy production, the relative conservation of EPA, and possibly a partial bioconversion of DHA to EPA during the starvation period in each species.     

Neuropathology and host-parasite relationship of acute experimental toxoplasmosis of the blue fox (Alopex lagopus)

The neuropathology and host-parasite relationship of experimental infection with the RH-strain of Toxoplasma gondii were studied in 27 blue foxes (Alopex lagopus) aged 0 to 23 days, using light microscopy, including immunohistochemical staining, and transmission and scanning electron microscopy. All cases displayed multifocal necrotic lesions with numerous parasitic tachyzoites in the brain and spinal cord. The gray matter and the meninges were most seriously affected. Although a wide variety of cell types were parasitized, neurons and astrocytes seemed to be the main target cells. Individual parasitophorous vacuoles usually contained only a few tachyzoites, with rosette formations as a prominent feature. The present ultrastructural study supports the theory that the parasites actively invade the host cells by mechanisms that are different from those of phagocytosis. This is apparently the first report indicating that the formation of the network of tubular structures within the parasitophorous vacuole of T. gondii is associated with a transient, sack-like formation in the posterior end of the tachyzoites.     

Enigmatic biodiversity correlations: ant diversity responds to diverse resources

A pattern noted in ecology is that diversity at one level begets diversity at other levels. In the case of consumers competing for similar resources, the diversity of those resources is thought to provide some degree of niche diversification in which a diverse set of consumer species can coexist. If, however, the diverse resources are not sufficiently distinct from one another, from the standpoint of the consumer species, such niche diversification will not exist. We experimentally show that a diverse array of twigs attracted 80% more species of twig-nesting ants than a monospecific collection of twigs. The specific tree species from which the twigs were derived did not explain the pattern. It appears that diversity per se at one level (twigs) creates conditions that promote diversity at another level (nesting ants).     

Glutamatergic and dopaminergic neurons mediate anxiogenic and anxiolytic effects of CRHR1

The corticotropin-releasing hormone receptor 1 (CRHR1) critically controls behavioral adaptation to stress and is causally linked to emotional disorders. Using neurochemical and genetic tools, we determined that CRHR1 is expressed in forebrain glutamatergic and γ-aminobutyric acid-containing (GABAergic) neurons as well as in midbrain dopaminergic neurons. Via specific CRHR1 deletions in glutamatergic, GABAergic, dopaminergic, and serotonergic cells, we found that the lack of CRHR1 in forebrain glutamatergic circuits reduces anxiety and impairs neurotransmission in the amygdala and hippocampus. Selective deletion of CRHR1 in midbrain dopaminergic neurons increases anxiety-like behavior and reduces dopamine release in the prefrontal cortex. These results define a bidirectional model for the role of CRHR1 in anxiety and suggest that an imbalance between CRHR1-controlled anxiogenic glutamatergic and anxiolytic dopaminergic systems might lead to emotional disorders.     

A slowly progressive retinopathy in the Shetland Sheepdog

Objective To describe a slowly progressive retinopathy (SPR) in Shetland Sheepdogs. Animals Forty adult Shetlands Sheepdogs with ophthalmoscopic signs of SPR and six normal Shetland Sheepdogs were included in the study. Procedure Ophthalmic examination including slit‐lamp biomicroscopy and ophthalmoscopy was performed in all dogs. Electroretinograms and obstacle course‐test were performed in 13 affected and 6 normal dogs. The SPR dogs were subdivided into two groups according to their dark‐adapted b‐wave amplitudes. SPR1‐dogs had ophthalmoscopic signs of SPR, but normal dark‐adapted b‐wave amplitudes. Dogs with both ophthalmoscopic signs and subnormal, dark‐adapted b‐wave amplitudes were assigned to group SPR2. Eyes from two SPR2 dogs were obtained for microscopic examination. Results The ophthalmoscopic changes included bilateral, symmetrical, greyish discoloration in the peripheral tapetal fundus with normal or marginally attenuated vessels. Repeated examination showed that the ophthalmoscopic changes slowly spread across the central parts of the tapetal fundus, but did not progress to obvious neuroretinal thinning presenting as tapetal hyper‐reflectivity. The dogs did not appear seriously visually impaired. SPR2 showed significantly reduced b‐wave amplitudes throughout dark‐adaptation. Microscopy showed thinning of the outer nuclear layer and abnormal appearance of rod and cone outer segments. Testing for the progressive rod–cone degeneration ( prcd )‐mutation in three dogs with SPR was negative. Conclusion Slowly progressive retinopathy is a generalized rod–cone degeneration that on ophthalmoscopy looks similar to early stages of progressive retinal atrophy. The ophthalmoscopic findings are slowly progressive without tapetal hyper‐reflectivity. Visual impairment is not obvious and the electroretinogram is more subtly altered than in progressive retinal atrophy. The etiology remains unclear. SPR is not caused by the prcd‐mutation.     

Do effects of soil compaction persist after ploughing? Results from 21 long-term field experiments in Sweden

The extent and persistence of the effect of soil compaction in a system with annual ploughing were investigated in 21 long-term field experiments in Sweden with a total of 259 location-years. Crop yield, soil physical properties and plant establishment were determined. All experiments had two common treatments: control (no extra traffic) and compacted (350 Mg km ha⁻¹ of experimental traffic in the autumn prior to ploughing), using a tractor and trailer with traditional wheel equipment and an axle load restricted to 4 Mg. During the rest of the year, both treatments were conventionally and equally tilled. The compaction was repeated each autumn for at least 7 years, and the yield was determined each year until 5 years after the termination of the compaction treatment.

Compaction decreased the porosity and the proportion of large pores and increased the tensile strength of dry aggregates. On clay and loam soils, it decreased the proportion of fine aggregates in the seedbed and the gravimetric soil water content in the seedbed.

The yield in the compacted treatment declined compared with the control during the first 4 years, after which it reached steady state. During this steady state, the compaction treatment caused a yield loss of 11.4%, averaged over 107 location-years. Within 4–5 years after the termination of the compaction treatment, the yield returned to the control level. The average yield loss at individual sites increased with increasing clay content.

Results from additional treatments indicated that yield loss was linearly correlated with the amount of traffic up to 300–400 Mg km ha⁻¹. With greater ground contact pressure or a greater soil water content at time of traffic, there was a greater yield loss.

Soil compaction effects on yield were similar for all spring-sown crops, and the percentage yield loss seemed to be independent of the yield. In a few location-years with winter wheat there was on average no yield decrease.

There were 5.1% less plants in the compacted treatment than in the control. The yield decrease was significantly correlated with the number of plants.

Between years results were highly variable, and no consistent correlations between yield loss and soil water content at the time of traffic or the weather conditions during the growing period were found. Soil compaction affected yield during years with good as well as poor conditions for crop growth.     

Pathological Lesions in Swine at Slaughter: V. Pathological Lesions in Relation to Some Environmental Factors in the Herds

The influence of environment on animal health was studied in 40 bacon herds. These herds delivered approx. 11,000 baconers annually to the slaughter-house. The data from the meat inspection was studied in connection with the environmental recordings, and the relationship between 18 lesions and 20 environmental factors were estimated by statistical methods. The data was collected in the period 1975–1977.The influence of environmental factors upon the prevalence of different lesions showed considerable variation. Ten environmental factors were significantly associated with pleurisy and tail lesions (cannibalism). On the other hand none of the environmental factors bore any significant relation to atrophic rhinitis, pericarditis, peritonitis, perihepatitis, polyarthritis, arthritis and claw lesions. Eight environmental factors influenced the proportion of healthy animals.The recorded environmental factors influenced the overall health situation to a varying degree. For example, design of pen walls, insulation of pen floors, occasional use of supplementary feed and free access to drinking water were related to 5–7 lesions, while the nature of the concentrate (meal/pellets) had no significant influence.The study indicated that many details in environment and management could be of importance to the health of bacon pigs. However, statistical analysis also showed that production systems had a considerably stronger influence upon the health situation than any of the “pure” environmental factors – even when these factors were added.