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21.
A 7-month-old-intact male domestic shorthair cat was presented with fever, anterior uveitis in the right eye and respiratory distress when handled. These signs along with mild changes in serum protein levels and the exclusion of other potential causes were suggestive of feline infectious peritonitis (FIP). As the disease progressed, more clinical signs consistent with FIP, including renal involvement and later pleural effusion, became evident. Non-pruritic cutaneous lesions, characterized by slightly raised intradermal papules over the dorsal neck and over both lateral thoracic walls, were recognized at the end stage of the disease. The identification of papules in well-haired skin was difficult, and clipping of the fur facilitated their detection. Definitive diagnosis of FIP was made by histopathology and by immunohistochemical demonstration of coronavirus antigen in macrophages within kidney and skin lesions. The case was classified as a mixed form of FIP. Recognition of associated cutaneous lesions may facilitate a diagnosis of FIP in suspicious cases. 相似文献
22.
Alyse Yeager John Commito Andrew Wilson Deborah Bower Lin Schwarzkopf 《Journal of pest science》2014,87(2):323-329
Invasive species management is a critical issue worldwide, but mitigation strategies are slow to develop, and invader populations often expand too rapidly for eradication to be feasible. Thus, reduction in numbers of individuals is the most heavily used management strategy for invasive pests. While long-term biocontrol agents may take years or decades to develop, simple trap modifications can increase capture of targeted demographic groups, such as ovigerous females. The present study identifies the effectiveness of trap modification and use of multiple attractants to capture the invasive cane toad (Rhinella marina). Cane toad traps typically use lights to attract insect prey. Studies suggest that adding a male cane toad advertisement call to attract toads by phonotaxis may be effective. The aims of this study were to determine whether (i) female capture efficiency was influenced by attractants in the same manner as male and juvenile captures, (ii) an acoustic attractant alone (without a light attractant) was sufficient to attract toads, and (iii) the location of an acoustic attractant (inside or on top of the trap) influenced trap success. Male toads were captured more frequently than females and juveniles; combining light and acoustic attractants increased toad capture; and placing the acoustic attractant inside the trap increased the capture of female cane toads. Removal of adult, ovigerous females is a promising strategy to slow population growth of invasive species. Our results suggest that using a sound attractant inside the trap with a UV light is most effective in targeting that particular cane toad cohort. 相似文献
23.
HIV-1 integrates into the host chromosome and persists as a provirus flanked by long terminal repeats (LTRs). To date, treatment regimens primarily target the virus enzymes or virus-cell fusion, but not the integrated provirus. We report here the substrate-linked protein evolution of a tailored recombinase that recognizes an asymmetric sequence within an HIV-1 LTR. This evolved recombinase efficiently excised integrated HIV proviral DNA from the genome of infected cells. Although a long way from use in the clinic, we speculate that this type of technology might be adapted in future antiretroviral therapies, among other possible uses. 相似文献
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25.
E. Thomas Scheuner Stefanie Schmidt Angelika Krumbein Ilona Schonhof Monika Schreiner 《植物养料与土壤学杂志》2005,168(2):275-277
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27.
Cassava mosaic disease (CMD) caused by a group of begomoviruses and transmitted by whitefly vector is a serious disease in all the cassava-growing areas of Africa. Field evaluation with replication was conducted in 2003 and 2004 in three agroecologies in Nigeria to study the response of 40 cassava genotypes to CMD and to investigate genotype × environment (GE) interactions on their reactions to CMD, using the rank-sum classification and site regression analysis model. The 40 genotypes were separated into resistant (n = 17), moderately resistant (n = 6), moderately susceptible (n = 2) and susceptible (n = 15) groups. Environments, genotypes and GE interactions were all highly significant (P < 0.0001) for the virus disease contributing 9.5%, 71.36% and 19.14%, respectively to total variation. More than 40% of the genotypes were identified as resistant to the disease. Genotypes TMS 98/0581, TMS 99/3073, TMS 97/4763, TMS M98/0040, TMS 98/0505, TMS 97/0211, TMS 97/4769, TMS 99/2123, TMS M98/0068 and TMS 97/0162 were shown to have high resistance to CMD. The study also identified Umudike, in south-east Nigeria, as having high disease severity and the most appropriate site for CMD resistance screening of genotypes. Most of the genotypes exhibited stable resistance to CMD. The implication that the availability of these resistant genotypes as identified in this study could be a source of CMD resistance for further breeding is discussed. 相似文献
28.
Albrecht Serfling Ilona Krämer Volker Lind Edgar Schliephake Frank Ordon 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(4):559-575
Breeding for resistance is an efficient strategy to manage wheat leaf rust caused by Puccinia triticina f. sp. tritici. However, a prerequisite for the directed use of Lr genes in breeding and the detection of new races virulent to these Lr genes is a detailed knowledge on Lr genes present in wheat cultivars. Therefore, respective molecular markers for 18 Lr genes were tested for specificity and used to determine Lr genes in 115 wheat cultivars. Results obtained were compared to available pedigree data. Using respective molecular markers,
genes Lr1, Lr10, Lr26, Lr34 and Lr37 were detected, but data were not always in accordance with pedigree data. However, leaf rust scoring data of field trials
confirmed the reliability of DNA markers. These reliable marker data facilitated the analyses of the development of virulent
leaf rust races from 2002 to 2009 based on released cultivars. A sudden change from low infection rates to susceptibility
was observed for Lr1, Lr3, Lr10, Lr13, Lr14, Lr16, Lr26 and Lr37 since 2006. Cultivars carrying several leaf rust resistance genes showed no significant shift to susceptibility except one
cultivar which revealed an increasing infection rate at a low level. In summary, it turned out that pedigree data are often
not reliable and a detection of Lr genes by diagnostic markers is fundamental to combine Lr genes in cultivars for a durable resistance against leaf rust, and to conduct reliable surveys based on released cultivars,
instead of ‘Thatcher’ NILs. 相似文献
29.
Lydka M Kopera-Sobota I Kotula-Balak M Chojnacka K Zak D Bilinska B 《Acta veterinaria Scandinavica》2011,53(1):12
Background
The dynamic cross-talk between epididymal cells is hormonally regulated and, in part, through direct cell-to-cell interactions. To date, no information is available regarding possible impact of anti-androgens on the proteins involved in the gap junctional communication within the boar epididymis. Thus, a question arised whether prenatal or postnatal exposure to an anti-androgen flutamide alters the expression of gap junction protein - connexin43 (Cx43) and androgen receptor (AR) expression in the caput, corpus and cauda epididymis and leads to delayed effects on morphology and function of adult pig epididymis.Methods
First two experimental groups received flutamide prenatally on gestational days 20-28 and 80-88 (GD20 and GD80) and further two groups were exposed to flutamide postanatally on days 2-10 and 90-98 after birth (PD2 and PD90). Epididymides were collected from adult boars. Routine histology was performed using hematoxylin-eosin staining. The expression of Cx43 and AR were analyzed using immunohistochemistry and Western blotting. Both analyses were supported by quantitative approaches to demonstrate the variations of the expression levels following the treatment. Apoptotic cells were identified using TUNEL assay.Results
Histological examination revealed differences in epididymal morphology of flutamide-exposed boars when compared to controls. Scarce spermatic content were seen within the corpus and cauda lumina of GD20, PD2 and PD90 groups. Concomitantly, frequency of epididymal cell apoptosis was significantly higher (p < 0.05) after exposure to flutamide at GD20. Moreover, in GD20, PD2, and PD90 groups, significantly lower AR expression (p < 0.05) was found in the principal and basal cells of the corpus and cauda regions, while in the stromal cells AR expression was significantly reduced (p < 0.05) along the epididymal duct. Concomitantly, a decrease in Cx43 expression (p < 0.05) was noticed in the stromal cells of the cauda region of GD20 and PD2 groups. This indicates high sensitivity of the stromal cells to androgen withdrawal.Conclusions
The region-specific alterations in the epididymis morphology and scarce spermatic content within the lumina of the corpus and cauda indicate that flutamide can induce delayed effects on the epididymal function of the adult boar by decrease in AR protein levels that results in altered androgen signaling. This may cause disturbances in androgen-dependent processes including Cx43 (de)regulation, however, we can not exclude the possibility that in response to flutamide decreased Cx43 expression may represent one mechanism responsible for functional disturbance of the boar epididymis. 相似文献30.
AAHA/AAFP Pain Management Guidelines Task Force Members Hellyer P Rodan I Brunt J Downing R Hagedorn JE Robertson SA 《Journal of Feline Medicine and Surgery》2007,9(6):466-480
Pain management in dogs and cats has undergone a dramatic evolution in the past decade. Current approaches focus on anticipation and prevention of pain, as well as both pharmacologic and non-pharmacologic management techniques. The veterinary team plays an essential role in educating pet owners about recognizing and managing pain in their pets. 相似文献