Rice-related greenhouse gases in Japan, variations in scale and time and significance for the Kyoto Protocol

The contribution of rice production to the three major greenhouse gases CO₂, CH₄ and N₂O in 1990, the base year of the Kyoto protocol is investigated for Japan. For the CO₂ assessment, we use a top-down life cycle approach, CH₄ is assessed using the Japanese GHG emission inventory and N₂O is assessed according to the ratio of rice area divided by the total area of agricultural soils. In total, 1.6% of greenhouse gas (GHG) emissions in 1990 originated from rice production. Next, we assess regional variations in nine rice-producing regions, based on the CO₂ data of 1990. General trends in rice production from 1960 to 2000 and data from the Japanese GHG emission inventory since 1990 are used to assess variations in time. The rice-related GHG emissions decreased to 1.05% of the total GHG emissions in 2001 and will be less than half the 1990 level in 2012, mainly due to the decrease in rice production. Contrary to the trend in GHG emissions of rice, overall GHG emissions increased as rice production fulfils important roles, in mitigating global warming and in adapting to changing climates. The protection of rice production is required to counter the increase of GHG emissions in transportation, waste and domestic sectors and to minimize problems related to landscape, water and natural hazard management.     

Comparing methods for handling missing values in food-frequency questionnaires and proposing k nearest neighbours imputation: effects on dietary intake in the Norwegian Women and Cancer study (NOWAC)

OBJECTIVE: To investigate item non-response in a postal food-frequency questionnaire (FFQ), and to assess the effect of substituting/imputing missing values on dietary intake levels in the Norwegian Women and Cancer study (NOWAC). We have adapted and probably for the first time applied k nearest neighbours (KNN) imputation to FFQ data. DESIGN: Data from a recent reproducibility study were used. The FFQ was mailed twice (test-retest) about 3 months apart to the same subjects. Missing responses in the test FFQ were imputed using the null value (frequencies = null, amount = smallest), the sample mode, the sample median, KNN, and retest values. SETTING: A methodological substudy of NOWAC, a national population-based cohort. SUBJECTS: A random sample of 2000 women aged 46-75 years was drawn from the cohort in 2002 (response 75%). The imputation methods were compared for 1430 women who completed at least 50% of the test FFQ. RESULTS: We imputed 16% missing values in the overall test data matrix. Compared to null value imputation, the largest differences in estimated dietary intake were seen for KNN, and for food items with a high proportion of missing. Imputation with retest values increased total energy intake, indicating that not all missing values are caused by respondents failing to specify no consumption, and that null value imputation may lead to underestimation and misclassification. CONCLUSION: Missing values in FFQs present a methodological challenge. We encourage the application and evaluation of newer imputation methods, including KNN, which may reduce imputation errors and give more accurate intake estimates.     

Changes in free amino acid and sugar levels of dried figs during aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus

An aqueous slurry of gamma-irradiated sterilized dried figs was inoculated with toxigenic strains of Aspergillus flavus and Aspergillus parasiticus. During incubation at 28 degrees C, pH, fructose, glucose, and free amino acids were determined by high-performance liquid chromatography and liquid chromatography (LC)/mass spectrometry, respectively, over 13 time points (1-20 days). At the same 13 time points using a LC/time-of-flight mass spectrometry screening method, aflatoxin B 1 and other secondary metabolites were simultaneously monitored. During the course of incubation, the pH significantly decreased and aflatoxin B 1 formation correlated with a reduction in proline content for both fungi. Of the 22 free amino acids that were monitored, only proline and cystine were found to be critical in supporting aflatoxin production. Levels of fructose and glucose steadily declined during incubation, until glucose was almost exhausted after 21 days. These time-course experiments confirmed the need for carbon and nitrogen sources for aflatoxin production in dried figs, and the favorable composition of figs as a fungal growth medium.     

Effect of partial harvesting strategies on Artemia biomass production in Vietnamese salt works

The effect of partial harvest strategies on the production of Artemia biomass was evaluated for 12 weeks under Vietnamese salt farm conditions. The initial stocking density was 100 nauplii L^?1. After 3 weeks of inoculation, Artemia adults were partially harvested at intervals of 1, 3, 6 and 9 days starting with an initial quantity of 30 kg ha^?1 day^?1 at first harvest, and then the quantity of harvestable biomass was adjusted according to the standing stock present in the culture pond, combined with the time needed to harvest these quantities and with the weight of biomass harvested in each pond. The results showed that in most cases, the total densities were not significantly different among harvesting frequencies (P>0.05). However, a relatively higher Artemia adult density and its standing stock were better maintained in the 3‐day than in the 1‐day interval, and were significantly higher compared with the other two harvesting frequencies. The total biomass yields were the highest (1587 kg ha^?1) in the 3‐day harvesting interval, followed by 1‐, 6‐ and 9‐day harvesting interludes, corresponding to 1323, 1091 and 975 kg ha^?1 respectively. However, no statistical difference was observed between the 1‐ and the 3‐day interval as well as between the 6‐ and the 9‐day harvest schemes (P>0.05). The results of this study suggest that partial harvest of Artemia biomass performed every 3 days appears to be an appropriate strategy to enhance biomass productivity.     

Atomic Force Microscopy (AFM) Study of Interactions of HMW Subunits of Wheat Glutenin

Atomic force microscopy (AFM) has been used to study the noncovalent interactions of alkylated HMW subunit 1Dx5 and a M _r 58,000 peptide derived from the central repetitive domain. Both protein and peptide align side‐by‐side to form fibrils, the HMW subunit forming a branched network, and the peptide forming linear rods. The N‐ and C‐terminal domains of the subunit would, therefore, appear to contain regions that interact through noncovalent interactions in the absence of disulfide bond formation. These regions may be of importance in facilitating disulfide bond formation during protein body development.     

Identification of Wap65, a human homologue of hemopexin as a copper-inducible gene in swordtail fish, <Emphasis Type="Italic">Xiphophorus helleri</Emphasis>

Copper is one of the major heavy metal pollutants found in the aquatic environment. Therefore, it is important for determining the genes that play a key role in copper metabolism in aquatic organisms. This study, thus, aimed to identify a new copper-inducible gene in swordtail fish, Xiphophorus helleri. Using ACP-based RT-PCR coupled with RLM-RACE, we cloned Wap65, a mammalian homologue of hemopexin gene. The gene exhibits high identity at amino acid levels with the Wap65 gene of other fish species (42–68%) and mammalian hemopexin gene (35–37%). In addition, ten cysteine and two histidine residues are conserved in the swordtail fish Wap65 gene. These cysteine residues are vital for structural integrity, and histidine residues provide high binding affinity towards heme. As revealed by RT-PCR, the gene was upregulated in swordtail fish that were exposed to copper in a dose- and time-dependent manner. Therefore, the identification of Wap65, a mammalian homologue of hemopexin, as a new copper-inducible gene will provide greater insight into the role of this gene in copper metabolism.     

Application of thermometric titrations to the study of soil organic matter. I. Simple polyelectrolytes

An introduction to the technique of thermometric titrimetry is provided and its special potentialities for the study of humic substances are discussed. As a prelude to the investigation of the complicated humic systems, the relatively simple polyelectrolytes, PAA and PMA, were first studied. Thermometric titration of these polymeric acids with sodium hydroxide solution gave good end points. Thermometric titration of the salts of these acids with various transition metal ions gave end point breaks corresponding stoichiometrically to charge neutralization of the polyelectrolyte. These thermometric titrations involving the transition metal ions are compared with the corresponding nephelometric titrations.