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41.
Two hand rasps (tungsten chip blade, solid carbide blade) and an electrically-driven solid carbide axial bur were used to treat the cheek teeth of 2 horses immediately postmortem. All teeth were normal and were rasped to a standard considered satisfactory in practice. Six teeth from each horse served as untreated controls. Following treatment, the teeth were extracted and the clinical crown removed and prepared for scanning electron microscopy. Teeth were also extracted and examined from a horse that had excessive dental treatment previously. Dental debris created by the procedures was collected and examined. All three rasp techniques resulted in amputation of odontoblast processes. The solid carbide blade cut deep gouges and grooves into the surface of the dentin, chipping the enamel and peripheral cement. No smear layer was created. Rasping with a tungsten chip blade created a partial smear layer and a smoother surface than the solid carbide blade. The electrically-driven bur produced a complete smear layer and removed all dental tissues to a smooth layer. The enamel had also been damaged by the electric bur. Crown particles collected after the procedures were larger following hand rasping compared with particles produced by the electric bur. The extent of damage to sensitive and vital dentin tissue was of concern. Further studies are required to establish the optimum technique for rasping equine cheek teeth. 相似文献
42.
The relative virulence for chickens of five strains of Pasteurella multocida was evaluated. Twenty groups, each of ten chickens, were inoculated with a standard dose of 10(5) of each of five strains by the intramuscular (I.m.), intravenous (I.v.), intratracheal (I.tr.) or conjunctival (Co) routes. The highest mortality occurred in the groups dosed I.m. and I.v., followed by I.tr. inoculation. The relative virulence of each strain did not change when inoculated by the different routes. The most virulent strain, VP161, caused 100% mortality by all except the Co route. The least virulent strain, VP17, caused a single mortality by the I.v. route, but gave a high level of protection to birds inoculated by both the I.m. and I.v. routes, when challenged by intramuscular injection with (VP161). There was no protection against I.m. challenge in the birds inoculated by the I.tr. or Co routes. Serum antibody levels measured by ELISA correlated with the level of protection against virulent challenge for groups inoculated I.m. or I.v., but not I.tr. Western blots of pooled sera from each group did not show any specific antigen recognition that might explain the observed differences in protection. Inoculation with strain VP17, (both I.m. and I.tr.) also gave a high level of protection to birds challenged with strain VP161 by intratracheal instillation. 相似文献
43.
Townsend KM Hanh TX O'Boyle D Wilkie I Phan TT Wijewardana TG Trung NT Frost AJ 《Veterinary microbiology》2000,72(1-2):69-78
A total of 36 tonsil swab samples were collected from healthy swine prior to slaughter at the abattoirs in Can tho and Tien giang provinces of Southern Vietnam. The presence of Pasteurella multocida in these samples was detected by the combination of direct cultivation and isolation, mouse inoculation and the polymerase chain reaction (PM-PCR). P. multocida was detected in 16 samples by PCR, with 17 strains ultimately isolated. All samples were negative for serogroup B by HSB-PCR and conventional serotyping, with isolates identified as A:3, D:1 or D:3. In addition, all samples were determined to be negative for the P. multocida toxin (PMT). Characterisation of isolated P. multocida by REP-PCR and biotyping revealed nine distinct REP profiles and seven biotypes among the 17 isolates. Some correlation was seen with P. multocida isolated from a previous Australian outbreak of acute swine pasteurellosis, and those isolated from fowl cholera outbreaks in Vietnamese poultry. 相似文献
44.
Roy D Berghaus Thomas B Farver Randall J Anderson John M Adaska Ian A Gardner 《Journal of veterinary diagnostic investigation》2006,18(3):233-242
Cows from 2 California dairies were tested for paratuberculosis at the end of lactation by using fecal culture and a commercially available serum enzyme-linked immunosorbent assay (ELISA) test kit. Individual cow characteristics and production variables were evaluated along with ELISA testing results as predictors of fecal culture status. In multivariable logistic regression analysis, age and a herd-standardized version of 305-day mature equivalent (305 ME) milk production were significant predictors of fecal culture status after adjusting for herd, quarter of the study year, and ELISA sample-to-positive (S/P) ratio. The area under a nonparametric receiver operating characteristic curve was significantly greater for a multivariable model that included age and the level of milk production when compared with a model without these covariates. In conclusion, consideration of cow-level covariates was useful as an aid in predicting Mycobacterium avium ssp. paratuberculosis (MAP) fecal culture status. For a given ELISA S/P ratio, older cows and those with lower 305 ME milk production relative to other cows in the herd were significantly more likely to be shedding MAP in their feces at the end of lactation. 相似文献
45.
Recent quantitative site-based monitoring and qualitative aerial and ground traverses provide contrasting assessments of the
health of much of the arid shrublands of Western Australia extensively grazed by livestock (‘rangelands’). Although these
results seem incompatible, we explain the apparent contradictions based on landscape succession processes operating at multiple
levels of ecological organisation. Specifically, we suggest that the intact areas in which site-based monitoring is conducted
are contracting as catchment canalisation and desiccation increase. However, the impacts of these processes have not yet become
manifest at the site scale. The site-based system addresses important regional questions. These relate to the large, relatively
intact areas away from most active surface flows, which should be a focus for resource conservation, given practical limits
to repairing widespread degradation with low management inputs. We provide a complementary set of questions to provide a more
comprehensive audit of rangeland dynamics in the context of underlying hierarchical landscape patterns and processes that
might threaten intact areas. We recognise the need to match questions and levels of ecological organisation and the implications
these have for sampling. We also recognise the difficulty in producing concise statements of change for clients when reporting
on complex ecological issues and processes. Without a clearly articulated, and well understood, hierarchical model of pattern
and process within which apparently contradictory findings can be reported meaningfully, policy makers may be confused by
the results, with the consequent risk of policy inaction. 相似文献
46.
Comparison of the Sensitivity of Landscape-fire-succession Models to Variation in Terrain, Fuel Pattern, Climate and Weather 总被引:1,自引:0,他引:1
Geoffrey J. Cary Robert E. Keane Robert H. Gardner Sandra Lavorel Mike D. Flannigan Ian D. Davies Chao Li James M. Lenihan T. Scott Rupp Florent Mouillot 《Landscape Ecology》2006,21(1):121-137
The purpose of this study was to compare the sensitivity of modelled area burned to environmental factors across a range of
independently-developed landscape-fire-succession models. The sensitivity of area burned to variation in four factors, namely
terrain (flat, undulating and mountainous), fuel pattern (finely and coarsely clumped), climate (observed, warmer & wetter,
and warmer & drier) and weather (year-to-year variability) was determined for four existing landscape-fire-succession models
(EMBYR, FIRESCAPE, LANDSUM and SEM-LAND) and a new model implemented in the LAMOS modelling shell (LAMOS(DS)). Sensitivity
was measured as the variance in area burned explained by each of the four factors, and all of the interactions amongst them,
in a standard generalised linear modelling analysis. Modelled area burned was most sensitive to climate and variation in weather,
with four models sensitive to each of these factors and three models sensitive to their interaction. Models generally exhibited
a trend of increasing area burned from observed, through warmer and wetter, to warmer and drier climates with a 23-fold increase
in area burned, on average, from the observed to the warmer, drier climate. Area burned was sensitive to terrain for FIRESCAPE
and fuel pattern for EMBYR. These results demonstrate that the models are generally more sensitive to variation in climate
and weather as compared with terrain complexity and fuel pattern, although the sensitivity to these latter factors in a small
number of models demonstrates the importance of representing key processes. The models that represented fire ignition and
spread in a relatively complex fashion were more sensitive to changes in all four factors because they explicitly simulate
the processes that link these factors to area burned.
The US Government's and the Canadian Government's right to retain a non-exclusive, royalty-free license is acknowledged 相似文献
47.
48.
Bras ID Colitz CM Saville WJ Gemensky-Metzler AJ Wilkie DA 《Veterinary ophthalmology》2006,9(5):317-327
Posterior capsular opacification (PCO) is the most common postoperative complication of contemporary cataract surgery. Limited information is available regarding PCO formation and factors that influence PCO development in the dog. Two hundred sixty-five eyes (144 from diabetic dogs and 121 from dogs with breed-related cataracts) were prospectively evaluated for PCO formation for up to 12 months postoperatively. The mean age of all dogs in the study was 7.77 years and diabetic dogs were significantly older than dogs with breed-related cataracts. There were 73 males (61 neutered, 12 intact) and 74 females (70 neutered, 4 intact) in the study. Statistical analysis was performed based on age, breed/size, gender, stage of cataract at the time of surgery, PCO score at each time point, breed-related vs. diabetic cataract, right eyes compared to left eyes, and presence/absence of uveitis. Age and gender did not significantly influence PCO formation. Small and medium-sized breeds developed significantly more PCO in comparison to the large/giant breeds at 2 weeks and 2-4 months postoperatively, but the differences were not significant at later time points. There was an overall significant increase in PCO formation in eyes with early immature cataracts when compared to other stages of cataract up to 4 months postoperatively but not at later time points. There were no statistical differences in PCO score at 6 months or at 1 year postoperatively in eyes with breed-related and diabetic cataracts. Right eyes did not differ from left eyes in PCO score. PCO score significantly increased over time in breed-related and diabetic groups and in the overall population. No difference was found in the degree of PCO formation in eyes with inflammation prior to or after surgery compared with those without inflammation. In summary, age, gender, presence of inflammation, and cause of cataract (breed-related vs. diabetes mellitus) do not influence the development of PCO in canine cataract dogs. Small and medium-sized breeds develop significant PCO earlier than larger breeds. It is important to note that all eyes from all dogs in this study developed PCO in a time dependent manner. 相似文献
49.
50.
Caroline N. Hahn Ian G. Mayhew Julia R. Washboume 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(4):310-312
Lateral thoracic nerve reflex latencies values were measured in ponies using a simple, non-invasive technique. The reflex was elicited using an external triggering hammer attached to an electrodiagnostic unit. The resulting evoked, compound muscle action potentials were recorded with electrodes, which were placed over the 6th ribs or 11th rib level with the axilla. Two superimposed repeats of 4 signal-averaged sweeps of 50 or 100 milliseconds were recorded and the estimated reflex pathway was measured for each subject in order to calculate the reflex latencies and latency velocities. Mean left and right 6th rib peak latencies were not significantly different from each other ( P = .609), but left 6th rib latencies were shorter than those recorded from the 11th rib ( P < .0001), substantiating the existence of an indirect (central) pathway to the reflex. The calculated left and right 6th rib latency velocities were not significantly different from each other ( P = .58) but left 6th rib latency velocities were different from left 11th rib ( P = .009). The calculated latency velocities were within the broad range for corticospinal tract motor conduction velocities and comparable to magnetic motor evoked latency velocities. The use of lateral thoracic reflex latency measurements to objectively identify the site of spinal cord lesions is discussed. 相似文献