Metabolism of flupyrazofos in the isolated perfused rat liver

To investigate the hepatic metabolism of the new insecticide flupyrazofos [O,O-diethyl O-(1-phenyl-3-trifluoromethylpyrazol-5-yl) phosphorothioate], isolated rat liver was perfused with flupyrazofos under single-pass conditions. In outflow perfusate and bile, 1-phenyl-3-trifluoromethyl-5-hydroxyprazole (PTMHP), PTMHP-sulfate and PTMHP-glucuronide conjugates were identified as the metabolites of flupyrazofos. However, O,O-diethyl O-(1-phenyl-3-trifluoromethylpyrazol-5-yl) phosphate (flupyrazofos oxon) was not detected. A HPLC method with UV detection was used to investigate the hepatic disposition of flupyrazofos and its metabolite PTMHP. The concentrations of flupyrazofos, PTMHP and PTMHP conjugates in outflow perfusate reached steady-state levels within 20 min after commencing perfusion of 7.3 microM flupyrazofos. At steady state, the mean extraction ratio of flupyrazofos was 0.93 (+/- 0.01) and clearance was 26.1 (+/- 0.2) ml min-1 which nearly approached perfusate flow rate (28 ml min-1). PTMHP accounted for 55.7 (+/- 5.8)% of eliminated flupyrazofos and was recovered as unchanged PTMHP, PTMHP-sulfate and PTMHP-glucuronide in the bile as well as the outflow perfusate.     

Effect of catheter size and injection rate of contrast agent on enhancement and image quality for triple‐phase helical computed tomography of the liver in small dogs

Rapid contrast injection is recommended for triple‐phase helical computed tomography (CT) of the liver. However, a large‐gauge catheter is needed for faster contrast injection and this is not practical for small breed dogs or cats. The purpose of this crossover group study was to evaluate applicability of a lower injection rate with a small‐gauge (G) catheter for triple‐phase hepatic CT in small dogs. Triple‐phase CT images were acquired for six beagle dogs using three protocols: an injection rate of 1.5 ml/s with a 24 G catheter, 3.0 ml/s with a 22 G catheter, and 4.5 ml/s with a 20 G catheter. Enhancement of the aorta, portal vein, and hepatic parenchyma was measured in each phase (arterial, portal, and delayed) and image quality was scored subjectively by two observers. Injection duration, time to scan delay, and time to peak enhancement were also recorded. Contrast injection duration decreased with a higher injection rate (n = 6, P ≤ 0.01), but time to peak enhancement and time to scan delay were not significantly affected by injection rates and catheter sizes. Contrast injection rate did not significantly affect aortic, portal, and hepatic enhancement. In addition, separation between each phase and quality of images was subjectively scored as good regardless of injection rate. Findings from the current study supported using an injection rate of 1.5 ml/s with a catheter size of 24 G for triple‐phase hepatic CT in small dogs (weight < 12 kg).     

Effect of heat treatment on the antioxidant activity of extracts from citrus peels

The effect of heat treatment on the antioxidant activity of extracts from Citrus unshiu peels was evaluated. Citrus peels (CP) (5 g) were placed in Pyrex Petri dishes (8.0 cm diameter) and heat-treated at 50, 100, or 150 degrees C for 10, 20, 30, 40, 50, and 60 min in an electric muffle furnace. After heat treatment, 70% ethanol extract (EE) and water extract (WE) (0.1 g/10 mL) of CP were prepared, and total phenol contents (TPC), radical scavenging activity (RSA), and reducing power of the extracts were determined. The antioxidant activities of CP extracts increased as heating temperature increased. For example, heat treatment of CP at 150 degrees C for 60 min increased the TPC, RSA, and reducing power of EE from 71.8 to 171.0 microM, from 29.64 to 64.25%, and from 0.45 to 0.82, respectively, compared to non-heat-treated control. In the case of WE from CP heat-treated at the same conditions (150 degrees C for 60 min), the TPC, RSA, and reducing power also increased from 84.4 to 204.9 microM, from 15.81 to 58.26%, and from 0.27 to 0.96, respectively. Several low molecular weight phenolic compounds such as 2,3-diacetyl-1-phenylnaphthalene, ferulic acid, p-hydroxybenzaldoxime, 5-hydroxyvaleric acid, 2,3-diacetyl-1-phenylnaphthalene, and vanillic acid were newly formed in the CP heated at 150 degrees C for 30 min. These results indicated that the antioxidant activity of CP extracts was significantly affected by heating temperature and duration of treatment on CP and that the heating process can be used as a tool for increasing the antioxidant activity of CP.     

尿素配施有机物料时土壤不同氮素形态的动态及利用

用盆栽试验研究了尿素配施有机物料时土壤不同氮素形态的动态和利用特征。结果表明：尿素与C／N比高的小麦秸秆配施时，降低了作物吸氮量；与C／N比低的鸡粪配施时，增加了作物的吸氮量。但尿素单施，尿素与小麦秸秆配施，与鸡粪配施的生物量并没有显著的差异。尿素的水解和硝化在14d以前完成。与单施尿素相比，尿素配施秸秆降低了土壤中NH4－N和NO3－N的含量，而配施鸡粪增加了它们的含量，这与作物吸氮量的变化是一致的。土壤中微生物氮也参与了对作物的供氮过程。作物对“老固定态铵”的利用能力很弱，施肥会显著的增加土壤固定态铵的含量，这些“新固定铵”在作物生长期能被吸收利用。几种处理氮肥利用率的顺序为：尿素＞尿素与鸡粪配施＞尿素与秸秆配施。     

SCARA并联机构拓扑分析与其低耦合度机型设计

根据基于方位特征(Position and orientation characteristics,POC)的并联机构拓扑结构设计理论与方法,首先,对已提出的一类7个具有较好实用价值的SCARA并联机构,进行了拓扑结构分析,揭示了其POC集、自由度(含驱动副选取)、过约束数、耦合度以及输入-输出运动解耦性等5个最主要的拓扑特征,且发现这些机构的耦合度均较大,为2,表明其运动学正解和动力学求解十分复杂;继而基于机构拓扑结构降耦原理,又对κ=2的这7个机构进行了拓扑结构降耦优化,得到了低耦合度(κ=1),而机构POC、自由度(Degree of freedom,DOF)等保持不变的实现SCARA运动的14个新机型,不仅丰富了实现SCARA运动的4-DOF三平移一转动机型库,而且降低了这些机构的运动学和动力学代数求解难度,而其数值解可用一维搜索法方便求得,从而为这一类SCARA并联机构的运动学和动力学分析、设计及应用提供了理论基础。     

县·乡镇两级尺度下耕地质量空间自相关分析——以湖北省广水市为例

[目的]从县级、乡镇级两级空间尺度上运用空间自相关分析(Moran’s I)方法定量研究广水市耕地质量的空间集聚性规律特征。[方法]基于空间自相关Moran’s I分析方法,以湖北省广水市为研究区,将耕地质量国家自然等指数、利用等指数和经济等指数作为空间变量,以耕地图斑作为空间单元进行空间自相关分析,从县级和乡镇级两级尺度上分析耕地质量空间差异性特征并进行两级对比分析。[结果]广水市耕地质量在空间分布上呈现出一定的集聚性规律,"中北部"的耕地质量较高,"西北角"耕地质量相对较低,其他地方耕地质量处于中等水平且分布较集中。广水市耕地质量在县级和乡镇级尺度上呈现出较强的空间自相关,说明在空间格局上呈现出很强的集聚态势。县、乡镇两级尺度上,耕地质量指数Moran’s I值均呈现出国家经济等指数国家利用等指数国家自然等指数的递减特征,三者均呈现显著的空间正相关集聚态势。[结论]耕地质量空间差异在不同尺度上有一定的敏感性和内部差异性特征,耕地质量空间差异特征研究可为土地整治、高标准基本农田划定等工作提供参考。     

茶叶叶绿素混合液浸提法提取条件优化

本文采用混合液浸提法制得叶绿素提取液,分别从混合液配比、提取的温度、提取时间和浸提液用量等方面进行了系统试验,并从叶绿素的提取量和提取液的稳定性方面比较了混合液提取法与传统的丙酮研磨法.结果表明,混合液浸提法提取叶绿素,提取完全,提取量高于传统法;而且,混合液浸提法制得的叶绿素提取液稳定性更好.试验总结出,混合液浸提法的最佳条件是:室温下,以丙酮∶乙醇∶水=3∶6∶1的混合液30 mL,避光提取1 h.     

淮北平原1966～2007年蒸发量变化趋势及其影响因素分析

选择安徽淮北平原五道沟实验站1966～2007年的逐年器皿蒸发、不同深度的潜水蒸发资料以及对应的气温、相对湿度、水汽饱和差、风速、日照时数等气象数据计算淮北平原40年来蒸发过程的变化趋势及其与气象因子的关系,分析其相互作用关系。结果表明,近40年来淮北平原温度呈上升趋势,年均温度的增幅逐渐减小有利于蒸发能力增加,而太阳辐射、风速等减少和相对湿度增加蒸发阻力,且后者作用大于前者。在此基础上建立以气象变化因子的蒸发能力预测模型,能很好地反映气候变化过程对水面蒸发、潜水蒸发的影响,有利于评估未来水量平衡关系分析和预测。     

Chemical Synthesis and Structure-Activity Relationship Study Yield Desotamide a Analogues with Improved Antibacterial Activity

Desotamides A, a cyclohexapeptide produced by the deep-sea-derived Streptomyces scopuliridis SCSIO ZJ46, displays notable antibacterial activities against strains of Streptococcus pnuemoniae, Staphylococcus aureus, and methicillin-resistant Staphylococcus epidermidis (MRSE). In this study, to further explore its antibacterial potential and reveal the antibacterial structure-activity relationship of desotamides, 13 cyclopeptides including 10 new synthetic desotamide A analogues and wollamides B/B1/B2 were synthesized and evaluated for their antibacterial activities against a panel of Gram-positive and -negative pathogens. The bioactivity data reveal that residues at position II and VI greatly impact antibacterial activity. The most potent antibacterial analogues are desotamide A4 (13) and A6 (15) where l-allo-Ile at position II was substituted with l-Ile and Gly at position VI was simultaneously replaced by d-Lys or d-Arg; desotamides A4 (13) and A6 (15) showed a 2–4-fold increase of antibacterial activities against a series of Gram-positive pathogens including the prevalent clinical drug-resistant pathogen methicillin-resistant Staphylococcus aureus (MRSA) with MIC values of 8–32 μg/mL compared to the original desotamide A. The enhanced antibacterial activity, broad antibacterial spectrum of desotamides A4 and A6 highlighted their potential as new antibiotic leads for further development.     

Sesame oleosin and prepro-2S albumin expressed as a fusion polypeptide in transgenic rice were split, processed and separately assembled into oil bodies and protein bodies

A recombinant polypeptide containing the precursor protein of a sesame storage protein, 2S albumin, fused to the C-terminus of a sesame oleosin was expressed in transgenic rice seeds under the control of a rice glutelin promoter. The recombinant polypeptide of 32 kDa, equivalent to the resultant molecular mass of sesame oleosin (15 kDa) and prepro-2S albumin (17 kDa), was detected in the endoplasmic reticulum fraction of maturing transgenic rice seeds, but not in the purified oil bodies or the soluble extract of transgenic seeds. However, sesame oleosin presumably fused with a 2 kDa C-terminal appendix originating from the signal sequence of prepro-2S albumin, was found in the purified oil bodies, and mature sesame 2S albumin apparently processed into two subunits (9 and 4 kDa) linked by disulfide bonds was detected in extracts of transgenic seeds. Immunogold labeling revealed that the sesame oleosin and 2S albumin were separately located in oil bodies and protein bodies of embryo cells of transgenic rice seeds. While sesame 2S albumin was also detected in protein bodies of endosperm cells of transgenic seeds, the co-expressed sesame oleosin, probably degraded due to the lack of oil bodies in this tissue, and was not detected. The results provide a new technique for introducing two recombinant polypeptides separately into rice oil bodies and protein bodies from one expression construct.