Development of a powder formulation of Pseudomonas fluorescens for control of rice blast

Pseudomonas fluorescens strain Pf1, inhibitory to the growth of the rice blast pathogen Pyricularia oryzae in vitro , was developed as a talc-based powder formulation. When rice seeds were treated with this formulation, the bacteria spread to roots, stems and leaves of the plants and protected against leaf infection by P. oryzae . When applied as a foliar spray, the bacteria survived on the leaves. The powder formulation controlled leaf blast under greenhouse conditions. In tests as a seed treatment and foliar spray in four field trials it effectively controlled the disease and increased grain yield.     

Computationally Assisted Structural Elucidation of Cembranoids from the Soft Coral Sarcophyton tortuosum

A persistent study on soft coral Sarcophyton tortuosum resulted in the characterization of two new cembranolides, tortuolides A and B (1 and 2), and a new related diterpene, epi-sarcophytonolide Q. Their structures were determined not only by extensive spectroscopic analysis but also by DFT calculations of ECD and NMR data, the latter of which was combined with statistical analysis methods, e.g., DP4+ and J-DP4 approaches. Anti-inflammatory and cytotoxicity activities were evaluated in this study.     

Effects of organic amendments on evolution of ethylene and other hydrocarbons from soil

Evolution of C₂H₄ from soils under anaerobic conditions was stimulated by amendment with cereal straw, but hay had little effect. Temporary restoration of aerobic conditions resulted in large increases in C₃ and C₄ hydrocarbons, with both straw and hay amendments. Several known products of the fermentative degradation of carbohydrate increased the evolution of hydrocarbons under anaerobic, and to a greater extent, under aerobic conditions. In particular, ethanol and butyric acid promoted the formation of C₂H₄ and propylene, respectively. The association between degradation products and C₂H₄ suggests that both may be implicated when root growth is adversely affected by the anaerobic decomposition of plant residues.     

Laboratory incubation studies of chlorophenoxyacetic acids in chernozemic soils

The relative persistence of 2,4-D, MCPA and 2,4,5-T in some Saskatchewan soils was assessed under laboratory conditions. Under moist conditions, 2,4-D and MCPA showed half-life times of between 14 and 41 days but the MCPA half-life was usually 1 or 2 days longer. 2,4,5-T exhibited a half-life period over twice the length of the other chemicals. The half-life times were directly correlated to microbial plate counts, the larger numbers of soil microorganisms being associated with shorter residence times. Half-lives depended on soil moisture content and the best moisture levels for chemical loss appeared to be just less than field capacity. The use of ¹⁴C in 2,4-D incubation studies showed that the initial cleavage of the 2,4-D molecule was associated with the ether linkage and was not a decarboxylation.     

Experimental hybridization in certain diploid mexican solanum species

S. bulbocastanum is highly interfertile with S. cardiophyllum. Through the F₁ to F₅ little breakdown due to sterility or lack of plant vigour occurred. F₁ plants of a 5-species hybrid involving S. pinnatisectum, S. trifidum, S. jamesii, S. bulbocastanum, and S. cardiophyllum could also be intercrossed. One-way compatibility was frequently expressed in progenies of these interspecific crosses. It appears to be cytoplasmically controlled, although nuclear factors may be present which interact with cytoplasmic factors. The group of Mexican diploid potatoes represented by these species is potentially interbreeding and shows evidence of introgression throughout its range in central and Western Mexico.Contribution No. 169, Canada Department of Agriculture Research Station, Fredericton, New Brunswick, Canada     

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