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C Y Kang T K Brunck T Kieber-Emmons J E Blalock H Kohler 《Science (New York, N.Y.)》1988,240(4855):1034-1036
The self-binding properties of a dominant idiotypic antibody (T15) and a minor idiotypic antibody (M603), both specific for phosphorylcholine, were examined as models of self-binding antibodies (autobodies). Observed differences in the self-binding affinity of T15 and M603 relate to variable sequence differences in their respective heavy and light chains. A molecular recognition theory based on the translation of coding and noncoding DNA strands was used to identify complementary amino acid sequences responsible for self-binding. The second hypervariable region of the heavy chain domain, extending into the third framework region, was predicted as the primary self-binding locus. Among peptides synthesized with different variable heavy and light chain regions, a 24-residue peptide spanning the second hypervariable and third framework regions of the heavy chain of T15 was nearly as effective as phosphorycholine in inhibiting the self-binding complexes. 相似文献
74.
可溶性大豆多糖对酸性乳饮料稳定性影响的研究 总被引:2,自引:0,他引:2
该文主要研究可溶性大豆多糖(SSPS)对配制型酸乳及发酵型酸乳饮料稳定性的影响.通过测定酸性乳饮料的离心沉淀率,分别研究单一使用SSPS及SSPS、CMC和HMP复配后对配制型酸乳饮料稳定性的影响.同时,还研究了单一使用SSPS及SSPS和CMC复配后对发酵型酸乳稳定性的影响.结果表明,配制型酸乳最终pH值为3.9时,添加0.4%的SSPS或添加O.3%的SSPS和O.1%的CMC,具有良好的稳定性;正交试验结果表明,复合添加0.3%的SSPS、0.05%的CMC、0.4%的.HMP配制型酸乳的稳定性较好;发酵型酸乳的最终pH值为4.0时,添加0.4%的SSPS或添加0.1%的SSPS和0.3%的CMC,具有良好的稳定性. 相似文献
75.
单位面积穗数是决定小麦产量的主要因素之一。针对人工清点小麦穗数的方法容易受主观因素影响、效率低和图像处理方法鲜有进行系统部署等问题,提出一种注意力模块(Convolutional Block Attention Module,CBAM)与YOLOv5相结合的CBAM-YOLOv5网络结构,通过对特征图进行自适应特征细化,实现更准确的单位面积穗数测量。该研究以本地采集小麦图像数据和网络公开小麦图像数据为数据集,设置输入图像分辨率为1 280,得到CBAM-YOLOv5模型,可以达到0.904的F1分数和0.902的平均精度,测试集计数的平均相对误差为2.56%,平均每幅图像耗时0.045 s,综合对比,CBAM-YOLOv5模型具有显著优势。模型放置于服务器,结合手机端软件和辅助装置,形成单位面积穗数测量系统,实现育种小区麦穗图像实时采集、处理和计数,计数的平均相对误差为2.80%,抗环境干扰性强。该研究方法与装置可以实现田间小麦单位面积穗数的实时在线检测,降低主观误差,具有较高的准确率及较强的鲁棒性,为小麦单位面积穗数快速、准确估测提供一种新的技术和装备支撑。 相似文献
76.
Hang T. Nguyen C.-H. Kang C.-J. Ma Kum-Chan Choi Jin Seog Kim Joung Hae Lee Ki-Hyun Kim 《Water, air, and soil pollution》2009,196(1-4):225-243
The ionic composition of total suspended particulate (TSP) and fine (PM2.5) fractions was investigated from an 1,100 site in the middle of Mt. Halla in Jeju Island, Korea from March to November 2006. The sum concentrations of cation and anion species in TSP fraction were 205 ± 170 and 183 ± 164 neq m?3, respectively, while those for PM2.5 as 118 ± 129 and 88.5 ± 89.3 neq m?3, respectively. In TSP, the concentration of the major ions changed in the order of SO4 2? > NH4 + > Ca2+ > Na+ > NO3 ? > Mg2+ > K+ > Cl?, while its PM2.5 counterpart as NH4 + > SO4 2? > Ca2+ > NO3 ? > Na+ > Mg2+ > K+ > Cl?. Inspection of the temporal variabilities of ionic components indicated that most ions peaked in spring or fall months. The back trajectory analysis showed that the atmospheric composition of the major ionic species was affected fairly sensitively by long-range transport from China under the favorable meteorological conditions. In contrast, the lowest ionic concentration levels were seen most abundantly, when air masses passed from South Sea. Hence, the analysis of ionic concentration data suggests that their distributions are controlled by the combined effects of various source processes including the most prominent Chinese origin and the meteorological condition favorable for such transport. 相似文献
77.
Man-Goo Kim Young-Min Hong Mi-Hee Kang Dong-Soo Lee Bo-Kyoung Lee 《Water, air, and soil pollution》2001,130(1-4):565-570
Atmospheric gases and particulates were collected using four-stage filter-pack in Chunchon from January through December in 1999. Particulate SO4 2? and NO3 ?, and gaseous HNO3, SO2 and NH3 were analyzed. Annual average concentration of SO4 2?(S), NO3 ?(S), HNO3 (g), SO2(g) and NH3(g) were 5.75µg/m3, 4.98µg/m3, 0.33ppb, 1.52ppb and 7.25ppb, respectively. Annual dry deposition fluxes were estimated using the measured concentration and dry deposition velocity published by other research group. Annual dry deposition of S was 287kg · (km)?2·y?1, which accounted for about 30% of total S deposition. For N deposition, dry deposition is predominant; about 70% of total N deposition was through dry process mostly as forms of NH3 and HNO3. 相似文献
78.
Zhou HF Xie C Jian R Kang J Li Y Zhuang CL Yang F Zhang LL Lai L Wu T Wu X 《Journal of agricultural and food chemistry》2011,59(7):3060-3065
Caper (Capparis spinosa L.) fruits have been widely used as food and folk medicine in the Mediterranean basin and in central and west Asia. In this study, two biflavonoids, isoginkgetin, and ginkgetin, together with three other flavonoids, were isolated from caper fruits. Their chemical structures were elucidated by spectroscopic analyses and comparison with literature. To our knowledge, isoginkgetin, ginkgetin and sakuranetin were identified in caper for the first time. Notably, it is also the first time that biflavonoids have ever been found in the Capparidaceae. Concentrations of the two biflavonoids were measured in caper fruits collected from four major growing areas in northwest China. The anti-inflammatory effects of the flavonoids from caper fruits were evaluated by secreted placental alkaline phosphatase (SEAP) reporter assay, which was designed to measure nuclear factor-kappa B (NF-κB) activation. Isoginkgetin and ginkgetin showed inhibitory effects in initial screen at 20 μM, while the effect of ginkgetin was much greater than that of isoginkgetin. In a dose-response experiment, the IC(50) value of ginkgetin was estimated at 7.5 μM, suggesting it could be a strong NF-κB inhibitor and worthy of study in vivo. 相似文献
79.
80.
The objective of the present study was to investigate the transport and removal of Escherichia coli, Bacillus subtilis, Staphylococcus aureus, bacteriophage MS2, and bacteriophage Phix174 in the soils and pyrophyllite-amended soils. Laboratory columns experiments were performed under saturated flow conditions. Our results showed that bacteriophages passed through the soils more easily than bacteria under the given experimental conditions (pulse injection?=?15 min, flow rate?=?0.5 mL/min, column length?=?20 cm, inner diameter?=?2.5 cm, pH?=?7.6, electrical conductivity (EC)?=?150.1 μS/cm); the log removals of bacteria were in the range of 0.44 to 1.72, whereas the log removals of bacteriophages were between 0.01 and 0.13. Our results also demonstrated that the transport of bacteria and bacteriophages in the soil columns could be reduced considerably in the presence of pyrophyllite. Under the same column experimental conditions above, the log removals for MS2 and Phix174 in 50% soil?+?50% pyrophyllite were 2.64 and 3.05, respectively, whereas the log removals in 100% pyrophyllite were 5.70 for MS2 and 5.10 for Phix174; those values were far greater than the log removals in 100% soil (MS2?=?0.063, Phix174?=?0.128). Additional column experiments (step injection, flow rate?=?0.3 mL/min, column length?=?30 cm, inner diameter?=?2.5 cm, solution pH?=?8.4, EC?=?39.8 mS/cm) showed that the log removals for B. subtilis (1.72) and Phix174 (1.48) in the pyrophyllite were greater than those in the soil (B. subtilis?=?1.41; Phix174?=?0.39). This study demonstrated that the pyrophyllite amendment method could be used for protecting groundwater from microbial contamination by animal carcass burial soils. 相似文献