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61.
A cross-sectional study to show Eperythrozoon ovis infection is prevalent in Western Australian sheep farms 总被引:4,自引:0,他引:4
A serological survey and risk factor study was conducted to estimate the prevalence of Eperythrozoon ovis infection in Western Australian weaner sheep, the prevalence of farms with infected sheep, and to identify factors affecting initiation and maintenance of infection on the farm. The study was conducted on 91 farms, purposively chosen from 41 randomly selected regional shires stratified by sheep number and rainfall zones. Twenty sheep were selected systematically from a mixed-sex flock on each farm and tested for serum antibody to E ovis using an enzyme-linked immunosorbent assay. Information on putative risk factors was collected using an interview questionnaire. Antibody to E ovis was detected in 4.5% of sheep on 47% of the farms sampled. The prevalence of E ovis infection in sheep was estimated at the 95% confidence level to be between 3.6 and 5.5%, and the prevalence of farms with infected sheep was estimated to be between 37.5 and 56.5%. Most farms with serological evidence of infection occurred in the Great Southern agricultural region (79.5%), south-east of Perth through to Albany (latitude 32 to 34 degrees S, longitude 116 to 120 degrees E), and in the Northern region (12.8%) surrounding Geraldton (latitude 29 degrees S, longitude 114 degrees E). There were significantly more farms (P less than 0.05) with evidence of infection in the Great Southern region compared to the Central region between Geraldton and Perth, and on farms in the region south compared to north of latitude 32 degrees S. None of the putative risk factors examined in the questionnaire were associated with serological evidence of infection on the farm.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
62.
SUMMARY Salmonellosis in sheep was studied on 7 commercial voyages from Australia to the Middle East and in one animal house study. Faecal excretion of salmonellas was not a good indicator of pathological changes in the gastrointestinal tract. The ratio of the proportion of sheep excreting salmonellas to the proportion with histological lesions of salmonellosis ranged from 1:1 to 23:1. Increasing excretion rates in our studies indicated that most sheep would be exposed to infection, although deaths from salmonellosis were not spatially or temporally clustered as would be expected if challenge alone was a sufficient cause. We considered that inappetence predisposed to death from salmonellosis, and that sheep with inappetence were likely to be randomly distributed aboard ship. In the animal house study, histological lesions of salmonellosis were exclusively in inappetent sheep although almost all sheep excreted salmonellas in faeces. Lesions of salmonellosis were found only in sheep that were seriously ill or had died, which suggested that, under the conditions of lot-feeding and sea transport, most sheep with enteric lesions are likely to die. Lesions were not found in feeding controls. Adrenal gland weights, an indicator of stress duration and severity, were used to examine the temporal sequence of events in the development of salmonellosis. Weights were greater in sheep that died of inanition than in controls, suggesting that inappetent sheep were already stressed for that reason. The presence of enteric lesions of salmonellosis was associated with further increases in adrenal gland weights. In a small proportion of cases, septicaemic salmonellosis developed rapidly and without adrenal gland hypertrophy suggesting that the sheep succumbed before the adrenal glands had time to enlarge. We concluded that sheep were predisposed to salmonellosis by inappetence and that stress was involved in lesion development. We considered that the development of enteric lesions of salmonellosis, in the live export system, inevitably led to death. 相似文献
63.
Inherited progressive spinal myelinopathy in Murray Grey cattle 总被引:1,自引:0,他引:1
In a breeding experiment conducted to determine the mode of inheritance of progressive spinal myelinopathy, semen from a Murray Grey bull which had previously sired affected calves was used to inseminate 120 cows. Female progeny were then inseminated with semen from the same bull. Of the 51 calves born, six (11.8%) had spinal cord lesions consistent with progressive spinal myelinopathy. From analysis of pedigrees and the results of the breeding experiment it was concluded that the condition was inherited as an autosomal recessive condition in Murray Grey cattle. 相似文献
64.
Three-dimensional gyrokinetic simulations of microturbulence in magnetically confined toroidal plasmas with massively parallel computers showed that, with linear flow damping, an asymptotic residual flow develops in agreement with analytic calculations. Nonlinear global simulations of instabilities driven by temperature gradients in the ion component of the plasma support the view that turbulence-driven fluctuating zonal flows can substantially reduce turbulent transport. Finally, the outstanding differences in the flow dynamics observed in global and local simulations are found to be due to profile variations. 相似文献
65.
RB Richards 《Australian veterinary journal》2009,87(4):121-124
This paper discusses the network of government, private and university veterinary laboratories in Australia and New Zealand and how it is adapting and evolving to meet the challenges it faces. It includes the mechanisms for standardisation of procedures, quality assurance, and the role of national reference laboratories hosted by state government laboratories. It also highlights the crisis in supply of veterinary diagnosticians, especially the declining numbers of veterinary pathologists. Recent positive changes include the setting up of the National Animal Health Laboratory Strategy and an initiative to empower State and Territory government laboratories to test for exotic diseases. The ideal outcome for Australia and New Zealand is a laboratory service that remains the gold standard around the world. 相似文献
66.
Comparison of two methods of seminal plasma removal on buffalo (Bubalus bubalis) sperm cryopreservation 下载免费PDF全文
RS Albuquerque R Morais AN Reis MS Miranda EAR Dias FM Monteiro CCP Paz M Nichi GKV Kawai CPF Della'Aqua FO Papa RB Viana LU Gimenes 《Reproduction in domestic animals》2017,52(5):905-910
Cryopreservation causes damage to spermatozoa, and methods minimizing this damage are therefore needed. Although much discussed, seminal plasma removal has become an alternative to improve sperm quality and viability after freezing and has been applied to different species in attempt to obtain good results. The objective of this study was to evaluate semen quality in buffaloes submitted to two methods for seminal plasma removal (filtration and centrifugation). Semen samples were collected from seven Murrah buffalo bulls (Bubalus bubalis) once a week for 8 weeks. Each ejaculate was divided into three groups: control (presence of seminal plasma), centrifugation and filtration. Sperm kinetics was evaluated with the computer‐assisted sperm analysis (CASA) system. Plasmalemma and acrosomal membrane integrity, mitochondrial membrane potential and reactive oxygen species (ROS) were measured by flow cytometry, and lipid peroxidation was evaluated by the thiobarbituric acid reactive substances (TBARS) assay. Seminal plasma removal did not improve sperm kinetics compared to the control group. Centrifugation increased the number of cells with damaged acrosomal membranes (0.77 ± 0.05) and filtration caused greater plasmalemma and acrosomal membrane damage (22.18 ± 1.07). No difference in the mitochondrial membrane potential was observed between groups. In contrast, ROS production was higher in the centrifugation group compared to the control and filtration groups, although no differences in TBARS formation were detected. In conclusion, seminal plasma removal did not improve the quality of thawed buffalo semen compared to control in terms of sperm kinetics, membrane integrity, mitochondrial membrane potential or lipid peroxidation. 相似文献
67.
Recent history has demonstrated that classical swine fever (CSF) epidemics can incur high economic losses, especially for exporting countries that have densely populated pig areas and apply a strategy of non-vaccination, such as The Netherlands. Introduction of CSF virus (CSFV) remains a continuing threat to the pig production sector in The Netherlands. Reducing the annual probability of CSFV introduction (PCSFV) by preventive measures is therefore of utmost importance. The choice of preventive measures depends not only on the achieved reduction of the annual PCSFV, but also on the expenditures required for implementing these measures. The objective of this study was to explore the cost-effectiveness of tactical measures aimed at the prevention of CSFV introduction into The Netherlands. For this purpose for each measure (i) model calculations were performed with a scenario tree model for CSFV introduction and (ii) its annual cost was estimated. The cost-effectiveness was then determined as the reduction of the annual PCSFV achieved by each preventive measure (ΔP) divided by the annual cost of implementing that measure (ΔC). The measures analysed reduce the PCSFV caused by import or export of pigs. Results showed that separation of national and international transport of pigs is the most cost-effective measure, especially when risk aversion is assumed. Although testing piglets and breeding pigs by a quick and reliable PCR also had a high cost-effectiveness ratio, this measure is not attractive due to the high cost per pig imported. Besides, implementing such a measure is not allowed under current EU law, as it is trade restrictive. 相似文献
68.
van Asseldonk MA van Roermund HJ Fischer EA de Jong MC Huirne RB 《Preventive veterinary medicine》2005,69(1-2):39-52
We constructed a stochastic bio-economic model to determine the optimal cost-efficient surveillance program for bovine tuberculosis. The surveillance programs differed in combinations of one or more detection methods and/or sampling frequency. Stochastic input variables in the epidemiological module described the dynamics of infection and the probability of detection. By means of an efficiency frontier, the trade-off between the expected cost and the epidemiological risk parameter relating to the outbreak size was evaluated. The surveillance scheme based on visual inspection of lesions on carcasses at slaughter was optimal given the current prevalence of the disease in the Netherlands if the objective was to minimise the expected costs. However, the efficient set also included two other schemes: slaughterhouse inspection in combination with GAMMA-interferon testing of blood samples and slaughterhouse inspection in combination with two-stage tuberculin testing. The choice ultimately will depend on the risk attitude of the decision-maker; a more-stringent surveillance scheme will be enforced if the expected outbreak size is to be constrained. In future scenarios, ELISA testing of bulk-tank milk in combination with the current slaughterhouse inspection procedure would outperform the surveillance scheme of solely slaughterhouse inspection if ELISA testing of bulk-tank milk becomes feasible. 相似文献
69.
van der Fels-Klerx HJ Sørensen JT Jalvingh AW Huirne RB 《Preventive veterinary medicine》2001,51(1-2):75-94
This paper describes a personal-computer-based model estimating the economic losses associated with clinical bovine respiratory disease in replacement heifers raised on individual dairy farms. The model is based on the partial-budgeting technique, and calculates the losses for two types of the disease separately: calf pneumonia and a seasonal outbreak. Model input includes farm-specific data such as the incidence of bovine respiratory disease, prices, and effects of the disease on the heifers' productivity. The input database was linked directly with the economic model. For all input parameters, default values used are available to the user and can be modified easily.Losses considered by the model include treatment expenditures and costs associated with increased mortality, increased premature culling, reduced growth, reduced fertility and reduced milk production in first lactation. Uncertainty is taken into account for parameters related to disease incidence, mortality and culling.Basic calculations for a typical Dutch dairy farm with 60% of the heifers (<3 months) affected, indicated total annual losses due to pneumonia average 31.2 per heifer present on the farm (range 18.4-57.1). The estimated losses for one seasonal outbreak with heifers up to 15-months old affected were 27.0 per heifer present (range 17.2-43.1). For both BRD types, the model's outcome was most sensitive to the number of heifers affected. Most of the parameters that had a major impact on the total losses were related to treatment or to the effects on the heifers' productivity.The model is user-friendly and flexible, and can be used as an interactive tool by farmers and veterinarians in the (economic) decision-making process regarding on-farm prevention and control of bovine respiratory disease. 相似文献
70.
A study was conducted to record the deaths that occurred during export, and to examine the temporal pattern of pellet feeding in the feedlot and ascertain the variation in mortality between groups of sheep from different farms. Between November 1985 and April 1987, 5 cohorts of sheep were selected on arrival at a feedlot before live export by sea from Western Australia to the Middle East. Each cohort consisted of 4,118 to 9,612 Merino wethers from 15 to 35 farms (lines). There were few deaths during trucking to the feedlot (rate 0.9, range 0.0 to 3.0 per 10,000 sheep) and during lot-feeding (rate 6.2, range 1.2 to 12.2 per 10,000 sheep). Most of the deaths occurred aboard ship (rate 210.7, range 152.7 to 271.5 per 10,000 sheep). The proportion of sheep that ate pelleted feed increased with increasing length of time in the feedlot. By the end of lot-feeding the percentage of sheep that had not eaten pellets was 23.3, 4.4, 1.2, 2.1 and 0.2 in a representative sample of each of the 5 cohorts. It was considered that offering hay throughout lot-feeding was undesirable for the purpose of maximising the number of sheep that ate pellets before loading on the ship. Half of the deaths aboard ship occurred in 25% of 133 lines of sheep in the 5 cohorts. In each cohort the median per cent death rate (and range) per line was 1.3 (0.3 to 11.9), 1.1 (0.0 to 5.7), 1.8 (0.0 to 6.8), 2.3 (0.0 to 14.3) and 0.9 (0.1 to 5.4).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献