马铃薯Y病毒云南昭通烟草分离物的检测及年度间差异比较

利用DAS-ELISA检测试剂盒检测昭通烟草脉斑病样品,结果表明存在马铃薯Y病毒O株系和马铃薯Y病毒N株系两个不同株系病毒。利用Sprimer和M4引物扩增、克隆、测序,得到长度为1 771 bp目的片段,该片段包含病毒的外壳蛋白基因序列、3′ UTR序列以及部分Nib基因序列;序列分析表明与湖南HN 2分离物（GenBank No. GQ200836）和美国NE 11分离物（GenBank No. DQ157180）核苷酸序列相似性均为98%,系统进化分析表明其具有较近的亲缘关系。     

利用MaxEnt预测橡胶南美叶疫病菌在全球的潜在地理分布

橡胶南美叶疫病菌在美洲地区引起橡胶树严重的落叶病害,是亚太地区国家重要的检疫性有害生物。本文在MaxEnt预测全球范围内气候条件适宜该菌存活区域的基础上,结合寄主分布推测该菌的潜在地理分布。预测结果显示,在54个天然橡胶种植国家或地区中,除了该菌目前分布国家或地区、几内亚比绍和马里,其余33个国家或地区气候适宜值大于10的区域为该菌的潜在地理分布区,加强这些区域的调查监测对保护当地橡胶种植有重要意义。     

Biological Control of Sclerotinia Diseases of Rapeseed by Aerial Applications of the Mycoparasite Coniothyrium minitans

Indoor and field experiments were conducted to evaluate the efficacy of applying the mycoparasite Coniothyrium minitans to the aerial parts of rapeseed plants at the flowering stage to control sclerotinia diseases caused by Sclerotinia sclerotiorum. Under controlled conditions, a petal inoculation technique was used to determine the effect of conidial suspensions of C. minitans on suppression of sclerotinia leaf blight. Results showed that C. minitans was effective in inhibiting infection initiated by ascospores of S. sclerotiorum on flower petals by restricting mycelial growth of the pathogen. Suppression of lesion development was related to the conidial concentration of C. minitans, with larger lesions at low concentration (5×10³conidia ml⁻¹), but smaller lesions at high concentration (5×10⁴ conidia ml⁻¹ or higher). When C. minitans-treated rapeseed leaves were inoculated with mycelia of S. sclerotiorum, C. minitans failed to prevent infection of leaves, but caused a significant reduction in number of sclerotia produced on the diseased leaves. No significant difference in efficacy was detected between the two isolates of C. minitans, LRC 2137 and Chy-1, on the two rapeseed cultivars, Westar (spring type) and Zhongyou 821 (winter type). Results of field trials showed a significant reduction of stem rot of rapeseed in four (1997, 1999, 2003 and 2004) out of five years by aerial application of C. minitans, compared with controls. No significant difference in suppressive efficacy was observed between the treatments of C. minitans (10⁶ conidia ml⁻¹), C. minitans (10⁶ conidia ml⁻¹) + benomyl (50 μg ml⁻¹) and benomyl (100 μg ml⁻¹) in 2003, and between the treatments of C. minitans (10⁶ conidia ml⁻¹), C. minitans (10⁶ conidia ml⁻¹) + vinclozolin (100 μg ml⁻¹) and vinclozolin (500 μg ml⁻¹) in 2004. Sclerotia of S. sclerotiorum collected from diseased plants in plots treated with C. minitans in 1999, 2000 and 2003, or with C. minitans + benomyl in 2003 were infected by C. minitans at frequencies ranging from 21.3 to 54.5%. This study concludes that aerial spraying of C. minitans is an effective method for controlling sclerotinia diseases of rapeseed.     

深圳红火蚁蚁巢密度、社会型研究及防治效果评价

本文报道了深圳红火蚁生境类型与蚁巢密度的关系,社会型的鉴别以及应用茚虫威对多后型和单后型、多后型共存地区的防治效果。红火蚁的蚁巢密度（巢/100 m2）与生境类型密切相关,在所调查的5类生境中,待建地的蚁巢密度最高,为3.81巢/100 m2,公路边的次之,为2.95巢/100 m2,城市绿地的最低,为0.63巢/100 m2。应用多元PCR技术对6个行政区社会型进行鉴别的结果表明,福田、龙岗、南山区为多后型与单后型共存,单后型与多后型的比仅为1∶(3~4)。罗湖、宝安、盐田3区全部为多后型;多后型地区的蚁巢密度（2.2巢/100 m2）比单后型地区（1.9巢/100 m2）的为高,但没有显著差异;应用茚虫威进行防治60 d后的调查结果表明,多后型地区的防治效果（97.8%）比单后型地区（96.7%）的要好,但没有显著差异。最后,本文对这些结果产生的原因及应用价值进行了讨论。     

中国苜蓿品种对黄萎病抗病性鉴定

 黄萎病(Verticillium albo-atrum Reinke & Berthold)是苜蓿的毁灭性病害,在欧洲常于播种当年就有许多植株发病,第二年末或第三年会有大批植株死亡,使苜蓿地失去利用价值。1976年以后,本病在北美流行,并造成巨大经济损失。     

野百合及其变种百合的形态分类研究

对广东省内5个居群140个单株样品基部成熟叶的叶型性状分析结果表明,凡同时符合叶长／基宽距〈2．0以及叶长／叶宽〈5．0两个条件的样品为变种百合（Lilium brownii F．E．Brown ex Miellez vaur．virdulum Baker）,否则为原变种野百合（L．brownii F．E．Brown ex Miellez）。这一标准简单易操作,可作为野百合及百合形态分类的量化标准。     

东莞森林公园建设与管理对策思考——以大岭山森林公园为例

文章根据东莞森林公园建设的背景,从东莞发展森林公园建设的特殊条件（经济发展和城市建设特点）出发,分析存在的主要问题,提出东莞森林公园建设和管理的对策。     

保幼激素类似物对红火蚁的作用研究进展

我国发现红火蚁已有4年,但防治技术、产品依然匮乏。为了改善、提高我国红火蚁的防治技术水平,本文就保幼激素类似物(JHA)对红火蚁的作用表现和防治效果进行了总结分析。JHA可以造成蚁后卵巢萎缩、产卵量减少,导致发育畸形和蚁群等级比例失调,如工蚁数量减少、生殖型幼虫比例以及处女蚁后数量增加等,并最终导致整个蚁群死亡。JHA杀虫剂活性高、低残毒、对环境污染小,在田间防治红火蚁效果彻底,并可有效地防止防治区红火蚁种群的再入侵（re invasion）,但JHA毒饵也存在着防治效果缓慢等问题。本文就克服此类问题作了初步的探讨。     

氟吡菌酰胺不同施药方式对水稻拟禾本科根结线虫的防治效果

为评价41.7%氟吡菌酰胺悬浮剂防治水稻拟禾本科根结线虫Meloidogyne graminicola的应用潜力,将氟吡菌酰胺与吡虫啉种衣剂混合后采用种子包衣法及喷洒法研究其对水稻拟禾本科根结线虫的田间防效,并测定了不同施药方法对水稻生长和产量的影响。结果表明,氟吡菌酰胺4.2、8.3、12.5 g（a.i.）/kg种子与吡虫啉18.0 g（a.i.）/kg种子混合包衣处理,播种后35 d其根结抑制率和防效分别为41.0%~51.8%和47.4%~58.6%,土壤中2龄幼虫减退率为38.6%~40.4%,显著高于单施吡虫啉18.0 g（a.i.）/kg种子处理。水稻播种后连续3次以氟吡菌酰胺250.2、375.3、500.4 g（a.i.）/hm²进行土壤喷洒,最后1次施药后7 d,其根结抑制率和防效分别为81.0%~89.9%和65.9%~74.3%,土壤中2龄幼虫减退率为65.4%~73.4%,均显著高于对照药剂克百威1 800.0 g（a.i.）/hm²处理。氟吡菌酰胺各处理对水稻苗期生长均有较好的保护作用,能显著提高千粒重和有效穗数,产量比空白对照增加50.0%~61.2%,保产效果显著。水稻播种后35 d采用氟吡菌酰胺对稻田进行1次喷洒,对水稻具有一定的保护作用,保产效果不明显。表明采用氟吡菌酰胺与吡虫啉混合包衣种子处理及在水稻苗期进行喷洒处理对水稻拟禾本科根结线虫防效显著,具有显著的保产效果。     

Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickensOA

Background: Many researches about in ovo feeding(IOF) of vitamin C(VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems.Results: Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase(GLO) expression was lower during pre-hatch development than that during p...