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Repair of injured soft and hard tissues in horses can benefit greatly from the use of regenerative therapies with mesenchymal stem cells (MSC). Vitamin-C and platelet-rich-plasma had been used for in vitro differentiation of MSC. This study was aimed to evaluate the effect of vitamin-C, platelet-rich-plasma and their combination on the in vitro differentiation of adipose horse MSC. We isolated MSC from horse fat and differentiated them in vitro into osteogenic and chondrogenic lineages, as demonstrated by specific staining and RT-qPCR of selected genes. Combining vitamin-C and plasma-rich-platelet positively affected the ability of MSC to differentiate in vitro into mesodermal lineages during 14 days of culture; this effect was not as marked when differentiation was attempted for 21 days. This provides valuable information on the effect of combined use of these molecules in regenerative therapies and their potential application along stem cells for lesions of musculoskeletal tissue in sport horses. 相似文献
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The authenticity of weed distribution maps prepared by the geostatistical estimation method, kriging, was investigated. We concentrated on the total number of weed plants and the dominant weed genus Veronica spp. in a 2.1-ha field of winter wheat. Different sampling distances were evaluated. Our analyses showed that kriging weed densities based on seedling counts collected in, 0.25.m2 circles in a sampling grid of approximately 10 m × 10 m gave good agreement with actual observations. Reducing the sampling grid to 20 m × 30 m gave poor agreement. New sampling methods combined with positioning devices and injection sprayers are discussed with reference to site-specific weed management. 相似文献