Extraction and analysis of organic cations from Acid solution with strong electric fields and mass spectrometry

High electric fields have been used to extract organic cations from solutions of small organic molecules in polyphosphoric acid. The cations have been analyzed by mass spectrometry. Production of ions by this method is shown to be related to ease of protonation by acid rather than to ionization potential.     

Lymphocyte transformation test in the calf]

Studies into the effects of the environment on health and performance of agricultural animals are of clear-cut relevance to farming practice. The theoretical and practical importance of lymphocyte transformation to any assessment of immunological reactivity under defined environmental conditions, therefore, is reported in this paper. Communicated is methodical experience obtained from isolation of lymphocytes from peripheral blood of calves, lymphocyte culturing, and morphological evaluation of lymphocyte transformation. Views are given on the suitability of the lymphocyte transformation test. The following results have been obtained: Centrifugation of defibrinated blood, using "Visotrast-370", is recommended for lymphocyte isolation. Morphological evaluation will ensure a high degree of precision when 1 x 10(6) cells in one millilitre culturing fluid are to be cultured over three days, the culturing fluid consisting of 20 per cent of autologous serum and 80 per cent of Eagle medium, antibiotics, and a portion of 1-vol.% of phytohaemagglutinin (Wellcome). The lymphocyte transformation test, for its methodical accuracy, is good enough to detect and identify environmental effects on immunological reactivity of calf. It is likely to reflect the immunological response to an antigen application.     

Tiering in suspension-feeding communities on soft substrata throughout the phanerozoic

Tiering of benthic marine suspension-feeding communities on soft substrata has varied throughout the Phanerozoic. Epifaunal tiering was most developed during the middle and late Paleozoic and the Triassic to Jurassic, with large-scale reductions in tiering occurring during the Permian-Triassic extinctions and after the Jurassic. Infaunal tiering reached its highest level of organization after the Paleozoic.     

Radiographic changes in the lungs of horses with exercise-induced epistaxis

This paper describes the distinctive radiographic changes detected in the dorso-caudal lungfields of four racing thoroughbreds recently affected by exercise-induced epistaxis. A diffuse but localized increase in density was seen in all four cases, which demonstrated a variation from a predominantly alveolar density to an interstitial pattern and finally to increased bronchial markings. Evolution of the radiographic pattern of the pulmonary densities appeared to be related to the time that had elapsed since the bleeding incident. The implications of the changing pattern and site of the densities are discussed.