东海区发展水产资源增养殖的设想

东海区海岸线和岛屿岸线分别占全国的34%和49.1%,面积大于500m~2的岛屿共3481个,潍涂面积占全国的46.7%。港湾众多,总面积25507 km~2,0～20 m 水深的浅海面积64461 km~2,约占全国50%。东海区栖息了许多名特优的渔业资源,海洋生物具有相当的丰度和广度,水文环境稳定,营养盐类丰富,初级生产力高,也是多种鱼类、虾类、蟹类的栖息、繁殖场所。东海区(包括福建省、浙江省、上海市和江苏省)1991年海水增养殖面积达1335 km~2,总产量48.0万 t。全区具有较大规模的海带育苗、紫菜育苗、贝类育苗、虾类育苗、海蜇育苗的场地和设备。鱼类育苗技术不断提高.现已进行了约80个种类的人工、半人工育苗及20个种类的增殖放流或移植、自然增殖的试验。海水养殖种类达40～50种。东海区得天独厚的具有高经济价值的鳗苗和中华绒螫蟹苗的天然苗种资源。东海区既是全国海水增养殖业的重要基地也希望取得周边国家和地区的支持和合作。提出了今后(2000年止)发展的具体设想内容及主要项目,以及应采取的措施等,具有指导意义。     

靖海湾和五垒岛湾放流增殖中国对虾的回游与分布

用烧结法标志中国对虾，在靖海湾和五垒岛湾进行增殖放流，研究其回游与分布规律。重捕资料表明，６月中旬幼对虾对湾内河口浅水区生活随体长增长，逐步向深水扩散；８月上，中旬主群陆续游向湾外，按地形走向向西南回游，部分向东或东南；１０月份回游对湾外水域的对虾有向东，东北或东南回游的现象。     

刺参与日本对虾池塘高效混养技术

刺参夏眠期间正是日本对虾生长的大好季节,将刺参和日本对虾混养,在7月份放养虾苗,严格控制养殖池内的杂鱼虾等,就可大大提高日本对虾和刺参的成活率,利用此二者在饵料和空间的互补性,可显著提高虾池的利用率和经济效益。     

Effects of lysine and methionine supplementation on growth,body composition and digestive function of grass carp (Ctenopharyngodon idella) fed plant protein diets using high‐level canola meal

A 76‐day feeding trial was carried out to evaluate the effects of Lysine and Methionine supplementation on growth and digestive capacity of grass carp (Ctenopharyngodon idella) fed plant protein diets using high‐level canola meal (CM). Fish with initial average weight 103.9 ± 0.6 g were fed three extruded diets. Fish meal (FM) diet was formulated as the normal control with 40 g kg^?1 FM and 300 g kg^?1 CM; CM diet was prepared by replacing all FM with CM (total 340 g kg^?1) without Lys or Met supplementation; CM supplement (CMS) diet was similar to CM diet but was supplemented with essential amino acids (EAA) to ensure the levels of Lys and Met similar to those in the FM diet. Feed intake, feed efficiency and specific growth rate of the grass carp fed CMS and FM diets were similar (P > 0.05), but higher than those of the grass carp fed CM diet (P < 0.05). The hepatosomatic index, relative gut length, intestosomatic index and intestinal folds height were significantly improved in fish fed FM and CMS diets as compared to CM diet (P < 0.05). Lower activities of trypsin, lipase and amylase in hepatopancreas were observed in fish fed CM diet (P < 0.05). Three hundred and forty gram per kilogram CM without Lys or Met supplementation significantly decreased trypsin, lipase and amylase mRNA levels in hepatopancreas (P < 0.05). These results indicated that the high supply of CM (340 g kg^?1) in plant protein (200 g kg^?1 soybean meal and 100 g kg^?1 cottonseed meal) diets decreased digestive ability through decreasing digestive enzyme activities and enzyme gene's expressions of grass carp, and these side effects can be reversed by supplementing Lys and Met. Therefore, CM could be high level used in a plant protein blend‐based extruded diet for grass carp as long as EAA were supplemented.     

Effects of supplemental phytic acid on the apparent digestibility and utilization of dietary amino acids and minerals in juvenile grass carp (Ctenopharyngodon idellus)

A feeding trial was conducted for 8 weeks to evaluate the effects of supplemental phytic acid (PA) on the apparent digestibility and utilization of dietary amino acids (AAs) and minerals in juvenile grass carp. Five experimental diets consisted of graded levels of PA (0.2, 4.7, 9.5, 19.1 and 38.3 g kg^?1, named as P0, P5, P10, P20 and P40). Triplicate groups of fish (initial weight, 22.37 ± 0.16 g) were fed twice daily (08:00 and 16:00 h). The crude protein content in whole body significantly (p < .05) decreased in fish fed with 19.1 and 38.3 g PA kg^?1 diet. Supplemental PA (>4.7 g kg^?1) significantly reduced the apparent digestibility coefficient (ADC) of AAs (Asp, Thr, Ser, Glu, Gly, Ala, Cys, Val, Met, Ile, Leu, Phe, Lys, Pro, His and Arg) and the ADC of minerals (P, Ca, Mg, Zn, Cu, Fe and Mn) in grass carp. The contents of minerals (P, Ca, Mg and Zn) in whole body and bone were also found to be significantly reduced in dietary PA > 4.7 g kg^?1, while the bone ash, serum Alkp and Zn contents were found to be significantly decreased when the PA supplementation level was above 9.5 g kg^?1, and the contents of serum Ca and Mg were found to be markedly altered in higher PA‐supplemented groups. The results indicated that supplemental PA decreased the apparent digestibility and utilization of AAs and minerals, and thus reduced the feed utilization of grass carp, suggesting that the level of total PA should be below 4.7 g kg^?1 in grass carp diet.     

中国对虾皮下及造血组织坏死杆状病毒（HHNBV）DNA探针的制备及应用

ＨＨＮＢＶ是１９９３年中国对虾暴发性流行病的主要病原之一。从纯化的病毒中提取ＤＮＡ，用ＥｃｏＲｌ限制性内切酶酶切成ＤＮＡ片段，与ＰＵＣ１８体外重组，建立ＨＨＮＢＶＤＮＡ文库。从中筛选出三个重组质粒（５＃、８＃、９＃），它们所插入的片段大小分别为：２．８Ｋｂ、０．８Ｋｂ、１．６Ｋｂ，它们分别用光敏生物素标记成探针，与感染ＨＨＮＢＶ的病虾ＤＮＡ呈阳性反应，以此利用制备的ＨＨＮＢＶＤＮＡ探针的高敏感性和特异性来检测虾病。     

Effects of surgically implanted dummy ultrasonic transmitters on physiological response of bighead carp Hypophthalmichthys nobilis

The study assessed the effects of surgically implanted dummy ultrasonic transmitters on physiological response of bighead carp Hypophthalmichthys nobilis in April 2011. Before the surgery, 15 blood samples were extracted randomly from 195 bighead carp samples, and then the rest of the fish were divided into three groups: (1) control group, handing but no tagging, (2) sham group, surgical procedure without implantation of transmitter and (3) surgery group, surgical implantation of transmitters. In 3 h, 24 h, 7 days and 14 days after surgery, 15 fish were extracted randomly from the three groups, respectively, for sampling. Then the plasma samples were analyzed, and physiological measures of stress response (cortisol, glucose), tissue damage [alanine aminotransferase (ALT) and aspartate aminotransferase (AST)] and nutritional status [total protein, globulin, albumin, triglyceride, cholesterol, and alkaline phosphatase (ALP)] were compared. The result showed that there was no significant difference between sham and surgery groups in 3 h, 24 h, 7 days and 14 days after surgery. When compared to the control group, there were significant increases in concentrations of plasma cortisol, glucose, ALT, AST, total protein and globulin of sham and surgery groups in 3 h after surgery. After 24 h, the levels of plasma cortisol, ALT, AST, total protein, globulin and ALP were elevated in both sham and surgery groups, whereas the levels of plasma glucose had declined to normal level and plasma albumin, cholesterol and triglyceride were significantly decreased in both sham and surgery groups. After 7 days, the levels of plasma glucose, albumin and cholesterol continued to decline, while the level of plasma ALT, globulin and ALP had declined but still remained higher for sham and surgery groups than control group; however, the plasma total protein level had returned to normal. After 14 days, there was no significant difference between the three groups. The above results showed that surgical implantation of ultrasonic transmitters had indeed caused significantly negative effects on the physiological response of bighead carp. However, all the negative influences on stress response, tissue damage and nutritional status had disappeared in 14 days after surgery, and the presence of transmitters had no significant negative impact. Therefore, the bighead carp should be temporary cultured at least 14 days for the recovery of physiological response and then released into the free water for the ultrasonic telemetry research.     

显微注射生长激素基因导入中国对虾（Penaeus chinensis）受精卵的研究

本实验采用显微微量注射方法，交羊生长激素基因导入中轻地虾受精卵，受精卵发育到蚤状幼体第三期后采样检测。ＰＣＲ检测结果表明：７个样品共９３尾幼体，有３个样品呈出阳性信号，基因转移比率至少在３％以上。同时斑点杂交结果也证明有两个明显阳性斑点。     

罗非鱼在不同盐度水体中的生长繁殖和鱼肉粗蛋白与氨基酸含量的比较

罗非鱼原产非洲淡水中,但越来越多的人认为其祖先起源于海洋,因为多数种具有耐盐能力,尼罗罗非鱼就是其中之一。本试验的目的在于观察尼罗罗非鱼在海水中养殖的可行性。 材料与方法 实验鱼取自实验室内同胎孵出的25天龄尼罗罗非鱼(Oreochromis niloticus)鱼苗99尾,实验分为淡水、咸淡水(1/2海水+1/2淡水)和海水三个饲养组,每组33尾鱼苗,平均个体重0.15克,分别养于三个容量相同(内径80厘米,高60厘米)的白色圆形玻璃钢水槽中。控温26℃±1℃),充气,投喂豆饼、浒苔和白菜。每周换水1—2次。每个月月底称量各组鱼之总重和计算存活鱼尾数。实验始于1983     

汾河太原段轮虫多样性及群落结构的动态变化

调查汾河太原段轮虫群落结构的时空变化,探讨轮虫群落结构动态变化规律及其与环境理化因子的关系,为汾河太原段浮游动物动态预测和多样性保护提供必要信息,从生态系统的角度客观评价水质污染状况与水体富营养化程度。在汾河太原段设置采样点7个,于2014年3-12月对轮虫和水体理化指标进行逐月调查。共检出轮虫98种,隶属3目21科33属。全年优势种是长肢多肢轮虫（Polyarthra dolichoptera）和螺形龟甲轮虫（Keratella cochlearis）。调查期间月均检出轮虫28种,轮虫丰度变化范围为153 ~ 1 440 个/L,生物量为0.08～0.89 mg/L;种类数、丰度和生物量均表现为枯水季较低,丰水季最高。群落结构相似性分析表明不同营养状态水体之间差异显著,轮虫丰度、生物量、Shannon-Wiener 多样性指数和Pielou均匀度指数均存在明显差异;中等营养条件的指示种为卵形无柄轮虫（Ascomorpha ovalis）和单趾狭甲轮虫（Colurella unicauda）,指示轻度富营养的指示种为柔软龟甲轮虫（Keratella delicata）,中等富营养水体的指示种则为臂尾轮属的种类。冗余分析结果显示环境变量对轮虫群落结构变异的解释率为 41.3%,水温、叶绿素a 、溶解氧、总氮等因子是影响轮虫时空分布的主要因素。轮虫群落结构特征可以指示水体的营养状态。