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211.
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论述了不同降水年份(1997年干旱、1998年降水正常)及不同改良措施对典型草原退化草地植物群落的影响,结果表明,各种退化草地改良技术对退化草地的植物群落均有明显的效果,改良草地的第一性生产力增加,植物群落结构发生变化,优良牧草增加,植物群落的盖度、高度增加明显. 相似文献
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215.
Familial Congenital Methemoglobinemia in Pomeranian Dogs Caused by a Missense Variant in the NADH‐Cytochrome B5 Reductase Gene 下载免费PDF全文
H. Shino Y. Otsuka‐Yamasaki T. Sato K. Ooi O. Inanami R. Sato M. Yamasaki 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):165-171
Background
In veterinary medicine, congenital methemoglobinemia associated with nicotinamide adenine dinucleotide (NADH)‐cytochrome b5 reductase (b5R) deficiency is rare. It has been reported in several breeds of dogs, but little information is available about its etiology.Objectives
To analyze the NADH‐cytochrome b5 reductase gene, CYB5R3, in a Pomeranian dog family with methemoglobinemia suspected to be caused by congenital b5R deficiency.Animals
Three Pomeranian dogs from a family with methemoglobinemia were analyzed. Five healthy beagles and 5 nonrelated Pomeranian dogs without methemoglobinemia were used as controls.Methods
Methemoglobin concentration, b5R activity, and reduced glutathione (GSH) concentration were measured, and a turbidity index was used to evaluate Heinz body formation. The CYB5R3 genes of the affected dog and healthy dogs were analyzed by direct sequencing.Results
Methemoglobin concentrations in erythrocytes of the affected dogs were remarkably higher than those of the control dogs. The b5R activity of the affected dogs was notably lower than that of the control dogs. DNA sequencing indicated that this Pomeranian family carried a CYB5R3 gene missense variant (ATC→CTC at codon 194) that resulted in the replacement of isoleucine (Ile) by leucine (Leu).Conclusions and Clinical Importance
This dog family had familial congenital methemoglobinemia caused by b5R deficiency, which resulted from a nonsynonymous variant in the CYB5R3 gene. This variation (c.580A>C) led to an amino acid substitution (p.Ile194Leu), and Ile194 was located in the proximal region of the NADH‐binding motif. Our data suggested that this variant in the canine CYB5R3 gene would affect function of the b5R in erythrocytes. 相似文献216.
Effects of Moringa oleifera silage on milk yield,nutrient digestibility and serum biochemical indexes of lactating dairy cows 下载免费PDF全文
B. Zeng J. J. Sun T. Chen B. L. Sun Q. He X. Y. Chen Y. L. Zhang Q. Y. Xi 《Journal of animal physiology and animal nutrition》2018,102(1):75-81
This study investigated the effects of Moringa oleifera (MO) as a partial substitute of alfalfa hay on milk yield, nutrient apparent digestibility and serum biochemical indexes of dairy cows. MO was harvested at 120 days post‐seeding. Fresh MO was cut, mixed with chopped oat hay (425:575 on a DM basis), ensiled and stored for 60 days. Sixty healthy Holstein dairy cows were allocated to one of three groups: NM (no MO or control), LM (low MO; 25% alfalfa hay and 50% maize silage were replaced by MO silage) or HM (high MO; 50% alfalfa hay and 100% maize silage were replaced by MO silage). The feeding trial lasted 35 days. The LM and HM diets did not affect dry matter (DM) intake, milk yield or milk composition (lactose, milk fat, milk protein and somatic cell count). The apparent digestibility of DM and NDF was lower for HM group than NM group. Additionally, there were no significant differences in serum biochemical indexes between the LM and NM groups. The HM group had lower serum concentrations of total cholesterol, high‐density lipoprotein cholesterol, and low‐density lipoprotein cholesterol and higher serum concentrations of urea than the NM group. The partial replacement of alfalfa hay (≤50%) and maize silage with MO silage had no negative effects on milk yield, in vivo nutrient apparent digestibility or serum biochemical indexes of lactating cows. 相似文献
217.
G. F. Difford D. W. Olijhoek A. L. F. Hellwing P. Lund M. A. Bjerring Y. de Haas 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2018,68(1):25-32
This study assessed the ranking of dairy cows using individual-level correlations for methane (CH4) emission on-farm using sniffers and in respiration chambers. In total 20 lactating dairy cows, ten Holstein and ten Jerseys were recorded using sniffers installed in milking robots for three weeks of lactation and subsequently in respiration chambers (RC) where they were each recorded on three occasions within the RC. Bivariate linear mixed models were used to determine the individual-level correlations (rI) between sniffer and RC phenotypes as proxies for genetic correlations. Despite differences in feeding and management, the predicted CH4 production from sniffers correlated highly with RC CH4 production rI?=?0.77?±?0.18 and CH4 breath concentration correlated nearly as well with RC CH4 production rI?=?0.75?±?0.20. These correlations between sniffers on-farm and RC demonstrate the potential of sniffers measurements as large-scale indicator traits for CH4 emissions in dairy cattle. 相似文献
218.
Barriers to and enablers of implementing antimicrobial stewardship programs in veterinary practices 下载免费PDF全文
219.
三峡库区消落带不同海拔狗牙根草地土壤微生物生物量碳氮磷含量特征 总被引:4,自引:0,他引:4
为探究库区消落带优势草本植物狗牙根人工植被恢复后土壤质量及肥力的变化,选择三峡库区消落带150, 160和170 m海拔人工构建狗牙根植被土壤为研究对象,并以裸地作为对照,测定土壤微生物生物量碳、氮、磷和相关理化性质。结果表明:(1)各海拔狗牙根微生物生物量均显著高于裸地,表明狗牙根人工植被构建对土壤微生物恢复具有重要意义。(2)土壤微生物生物量碳、氮含量160 m高程显著高于150和170 m高程,但土壤微生物生物量磷(SMBP)却显著低于150和170 m高程,需要特别注意P元素的下移,加强水体P含量的检测。(3)土壤微生物生物量碳氮比和碳磷比变化范围分别为5.93~15.62和7.11~19.99,土壤微生物生物量碳、氮、磷占土壤有机碳、全氮、全磷百分比的范围分别为0.68%~2.57%、0.68%~3.33%、1.95%~5.23%。狗牙根土壤微生物生物量碳氮比和碳磷比显著低于裸地,表明狗牙根土壤有效氮、有效磷高于裸地;土壤微生物生物量碳、氮、磷占土壤有机碳、全氮、全磷百分比显著高于裸地,表明狗牙根土壤营养元素周转速率快于裸地。(4)微生物生物量碳、氮、磷与土壤有机碳、全氮和土壤含水率有显著或极显著相关性,与土壤pH值呈不同程度的负相关。因此,在三峡库区消落带进行狗牙根草地恢复重建能显著提高土壤微生物生物量及土壤质量,对加强三峡库岸生态系统的稳定性具有重要意义。 相似文献
220.
Immunohistochemical differentiation of reactive from malignant mesothelium as a diagnostic aid in canine pericardial disease 下载免费PDF全文
E. Milne Y. Martinez Pereira C. Muir T. Scase D. J. Shaw G. McGregor L. Oldroyd E. Scurrell M. Martin C. Devine H. Hodgkiss‐Geere 《The Journal of small animal practice》2018,59(5):261-271