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OBJECTIVE: To examine the effects of an aerosolized beta2-adrenoreceptor agonist, albuterol, on performance during a standardized incremental exercise test in clinically normal horses. ANIMALS: 8 Standardbred pacing mares. PROCEDURE: Clinically normal horses, as judged by use of physical examination, hematologic findings, serum biochemical analysis, and airway endoscopy, were randomly assigned to 2 groups and were given 900 microg of albuterol via a metered-dose inhaler 30 minutes before beginning a standardized incremental exercise test in a crossover design with a 7-day minimum washout. Further examination included measurement of baseline lung mechanics, response to histamine bronchoprovocation, and bronchoalveolar lavage. RESULTS: No significant differences (albuterol vs placebo) were seen for any incremental exercise test variables (ie, maximum oxygen consumption, maximum carbon dioxide consumption, respiratory quotient, treadmill speed at heart rate of 200 beats/min, or number of steps completed during an incremental exercise protocol). Mast cell percentage was significantly (r = -0.84) associated with the concentration of aerosolized histamine that evoked a 100% increase in total respiratory system resistance. No other direct correlations between bronchoalveolar lavage fluid cell types and any indices of exercise capacity or airway reactivity were found. CONCLUSIONS AND CLINICAL RELEVANCE: Although no horse had exercise intolerance, 4 horses had airway hyperreactivity with bronchoalveolar lavage fluid mastocytosis; these horses may have been subclinically affected with inflammatory airway disease. In our study, albuterol did not enhance performance in 8 clinically normal racing-fit Standardbreds. 相似文献
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Neospora-like protozoan infection as a major cause of abortion in California dairy cattle 总被引:17,自引:0,他引:17
M L Anderson P C Blanchard B C Barr J P Dubey R L Hoffman P A Conrad 《Journal of the American Veterinary Medical Association》1991,198(2):241-244
Ninety-five aborted bovine fetuses received from California dairies over a 4.5-year period had histologic lesions of focal encephalitis. Protozoa that reacted with Neospora caninum antiserum were detected in the brain of 88 of these fetuses and in the heart of 1 fetus. Sarcocystis spp schizonts were seen in the vascular endothelium of 1 fetus. It was concluded that a Neospora-like cyst-forming coccidian may be a major cause of abortion in California dairy cattle. 相似文献
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Leaching requirement, the smallest steady-state leaching fraction which prevents any loss in crop yield, was determined for barley, cowpea, and celery in field plots at the U.S. Salinity Laboratory. Six replicated leaching-fraction treatments were irrigated many times each day with small quantities of water having an electrical conductivity of 2.3 dS/m. The crops were grown in succession between January 1979 and September 1981.The leaching requirement (Lr) was 0.10 for barley grain and 0.13 for barley forage. For cowpea seed, Lr was 0.16; 0.17 for cowpea forage. Lr for celery was 0.14. These experimentally determined values for barley and cowpea seed are higher by about 0.05 than those predicted by a leaching-requirement model based on an exponential crop water-uptake pattern. The experimental values for celery and cowpea forage are lower than predicted values by 0.06. These differences are not considered significant, however, when considered in terms of the small differences in water applications (about 25 mm) to cause these changes in Lr. Evapotranspiration during each crop's growing season coincident with Lr was 410, 630, and 460 mm for barley, cowpea, and celery, respectively. 相似文献
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Kroll JJ Roof MB Hoffman LJ Dickson JS Harris DL 《Animal health research reviews / Conference of Research Workers in Animal Diseases》2005,6(2):173-197
Proliferative enteropathy (PE; ileitis) is a common intestinal disease affecting susceptible pigs raised under various management systems around the world. Major developments in the understanding of PE and its causative agent, Lawsonia intracellularis, have occurred that have led to advances in the detection of this disease and methods to control and prevent it. Diagnostic tools that have improved overall detection and early onset of PE in pigs include various serological and molecular-based assays. Histological tests such as immunohistochemistry continue to be the gold standard in confirming Lawsonia-specific lesions in pigs post mortem. Despite extreme difficulties in isolating L. intracellularis, innovations in the cultivation and the development of pure culture challenge models, have opened doors to better characterization of the pathogenesis of PE through in vivo and in vitro L. intracellularis-host interactions. Advancements in molecular research such as the genetic sequencing of the entire Lawsonia genome have provided ways to identify various immunogens, metabolic pathways and methods for understanding the epidemiology of this organism. The determinations of immunological responsiveness in pigs to virulent and attenuated isolates of L. intracellularis and identification of various immunogens have led to progress in vaccine development. 相似文献
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Hoffman AM Swanson LG Bruns SJ Kuehn H Bedenice D 《American journal of veterinary research》2005,66(7):1167-1174
OBJECTIVE: To determine whether tension of the girth strap of a saddle would sufficiently affect rib motion and reduce lung volume to alter pulmonary resistance in horses. ANIMALS: 10 healthy adult horses. PROCEDURE: We used classical techniques to measure the effects of tightening a girth strap (15 kg of tension) on pulmonary dynamics during eupnea and hyperpnea in horses. Respiratory impedance was evaluated by use of oscillometry, and resistance and reactance data were partitioned into lung and chest wall components. Rib cage and abdominal contributions to tidal volume and minute ventilation were measured by use of respiratory inductance plethysmography. Effects of strap tension on functional residual capacity (FRC) were measured during eupnea by use of a helium-dilution technique. In a subgroup of 6 horses, we also measured transdiaphragmatic pressures during eupnea and hyperpnea induced by administration of lobeline hydrochloride (0.2 mg/kg, i.v.). RESULTS: Pulmonary resistance measured by use of oscillometry but not by use of classical methods was significantly increased by the tension of the girth strap. However, the increase in pulmonary resistance could not be explained by a decrease in FRC. Motion of the rib cage was significantly reduced during eupnea and hyperpnea. However, ventilatory variables (tidal volume, minute ventilation, and peak flows), FRC, and transdiaphragmatic pressures were unaltered by strap tension. CONCLUSIONS AND CLINICAL RELEVANCE: Although tension of the girth strap caused measurable changes in respiratory mechanics (loss of rib motion and increased pulmonary resistance), there was no evidence that ventilation was limited. 相似文献