Using farmers' knowledge for defining criteria for land qualities in biophysical land evaluation

The objective of this paper is to present a way of complementing empirical results with farmers' perceptions in defining limiting biophysical land properties in a land suitability evaluation using the FAO framework methodology. The farmers' perceptions were identified using rapid and participatory rural appraisal (RRA/PRA) tools. The study catchment, having a semiarid continental climate and located on the Loess Plateau in northern China, covered an area of 3.5 km². Most of the land users were dependent on subsistence agriculture. There were important topographic variations in the catchment and arable cropping on steep slopes brought about degradation of land due to water erosion. The biophysical monitoring, soil survey and RRA/PRA survey, carried out one year prior to the present investigation, supplied the data needed for identification of preliminary limiting land properties and land evaluation units. The land properties that needed further investigation in the present study were slope aspect, soil workability, flooding hazard and farmers' criteria on choice of land‐use type. The farmers were able to give a comprehensive picture of the spatial and temporal variation and the importance for land‐use options of the land properties concerned, and thereby complement the information gained from empirical results (measurements). In order to guarantee good production for dry as well as wet years, both south‐ and north‐facing sites were chosen for most crops, and the slope aspect did not need to be differentiated in the final land suitability evaluation for arable crops. Grassland, however, was considered to be more suitable than woodland on south‐facing sites. Hard soil layers were found to be important, since they affected soil workability and erosion negatively, giving slightly reduced suitability for the land units in which they occurred. Flooding events affecting crops on alluvial soils negatively were considered to occur once every 5 to 10 years, which is considered to be a low rate, so this property was therefore not included in the final suitability evaluation. Copyright © 2001 John Wiley & Sons, Ltd.     

Spawning behaviour of Penaeus (Fenneropenaeus) merguiensis de Man and the effect of light intensity on spawning

An experiment was conducted to investigate the effect of light intensity just prior to and during spawning of Penaeus (Fenneropenaeus) merguiensis de Man in the laboratory. Neither the presence of light nor light intensity (0, 10 and 1100 lux) had a significant effect on the spawning process or hatching rate in P. merguiensis. Difference in the percentage of spawners, percentage of incomplete spawning, fecundity and hatching rate were all insignificant (P > 0.05) among the three light treatments. However, spawning was delayed (up to 21 min on average) under 1100 lux with a high percentage of incomplete spawning, compared with the Control (0 lux). For the first time, spawning behaviour of P. merguiensis was observed and fully described. The spawning process can be divided into four phases: dormant, pre‐spawning, spawning and post‐spawning by distinct behaviour of prawn broodstock. The dormant phase was characterized by a quiescent and non‐feeding period, whereas movement of prawn broodstock was active during the pre‐spawning and post‐spawning phases. Depending on the individual, spawning was completed within 3–5 min. Prawns often stayed still on the water surface during spawning with occasional manoeuvres to change the position. After a short rest at the termination of the spawning phase, prawn broodstock re‐exhibited active movement as in the pre‐spawning phase, but showing conspicuous care to the deposited eggs. The role of light in controlling ovarian development and spawning, and the behaviour of prawn broodstock in relation to maximizing fertilization rate are discussed.     

The determinants of detecting veterinary drug residues: Evidence from shrimp farmers in southern Viet Nam

While export-oriented shrimp farming has become an important source of income for many small-scale farmers in developing countries, the rate at which products are rejected at the ports of developed countries has remained high mainly due to the overuse of antibiotics. To reveal what determines the overuse of antibiotics, we interviewed 201 shrimp farmers in Viet Nam in 2015 and collected shrimp samples from each household’s pond for the screening of residual drugs. These tests revealed that residual drugs exceeding acceptable limits by Japanese standards were found in the samples of 40 farmers. We conducted cross-sectional Logit and Tobit regressions to examine whether results of the residue tests are significantly associated with farmers’ characteristics and management practices. This study finds that: (1) receiving BMP training has a significant and positive effect on reducing residual drugs; (2) if farmers know multiple extension officers, these relationships have significant and positive effects on reducing residual drugs; and (3) farmers with experience of shrimp disease outbreaks reduce use of antibiotics. Overall, this study contributes to revealing the determinants of detecting veterinary drug residues in shrimp farming in multiple (social, economic and psychological) dimensions.     

Sodium fluoride influences calcium metabolism resulting from the suppression of osteoclasts in the scales of nibbler fish <Emphasis Type="Italic">Girella punctata</Emphasis>

The influence of sodium fluoride (NaF) on calcium metabolism was examined in nibbler fish (marine teleosts). Two days after the administration of NaF (5 μg/g of body weight) (around 10^?4 M in fish), we showed that plasma calcium levels significantly decreased in NaF-treated nibbler fish. In addition, we detected fluoride in the treated scales by use of a scanning electron microscope with an energy-dispersive X-ray microanalysis, indicating that NaF directly affects their scales. Therefore, the influence of NaF on osteoblasts and osteoclasts in the scales was examined. In the scales of NaF-injected nibbler fish, tartrate-resistant acid phosphatase (TRAP) (osteoclastic marker enzyme) decreased, although alkaline phosphatase (osteoblastic marker enzyme) was activated. To confirm the effect of NaF on osteoclasts, furthermore, the mRNA expressions of osteoclastic markers (matrix metalloproteinase-9 and TRAP) were decreased significantly 2 days after incubation. In barred knifejaws, plasma calcium levels decreased as they did in nibbler fish. Therefore, NaF functions in both osteoblasts and osteoclasts and then influences calcium metabolism in marine fish. In the marine environment, high levels of fluoride (1.2–1.5 mg F^?/l) (around 10^?5–10^?4 M) are present in seawater. It is probable that teleosts living in seawater efficiently use fluoride to regulate their blood calcium levels.     

Experimental assessment of the effects of sublethal salinities on growth performance and stress in cultured tra catfish (Pangasianodon hypophthalmus)

The effects of a range of different sublethal salinities were assessed on physiological processes and growth performance in the freshwater ‘tra’ catfish (Pangasianodon hypophthalmus) juveniles over an 8-week experiment. Fish were distributed randomly among 6 salinity treatments [2, 6, 10, 14 and 18 g/L of salinity and a control (0 g/L)] with a subsequent 13-day period of acclimation. Low salinity conditions from 2 to 10 g/L provided optimal conditions with high survival and good growth performance, while 0 g/L and salinities >14 g/L gave poorer survival rates (p < 0.05). Salinity levels from freshwater to 10 g/L did not have any negative effects on fish weight gain, daily weight gain, or specific growth rate. Food conversion ratio, however, was lowest in the control treatment (p < 0.05) and highest at the maximum salinities tested (18 g/L treatment). Cortisol levels were elevated in the 14 and 18 g/L treatments after 6 h and reached a peak after 24-h exposure, and this also led to increases in plasma glucose concentration. After 14 days, surviving fish in all treatments appeared to have acclimated to their respective conditions with cortisol levels remaining under 5 ng/mL with glucose concentrations stable. Tra catfish do not appear to be efficient osmoregulators when salinity levels exceed 10 g/L, and at raised salinity levels, growth performance is compromised. In general, results of this study confirm that providing culture environments in the Mekong River Basin do not exceed 10 g/L salinity and that cultured tra catfish can continue to perform well.     

基因工程菌枯草芽孢杆菌GEB3产生的脂肽类抗生素及其生物活性研究

 利用MALDI TOF MS技术 ,鉴定了将lpaB3基因转入枯草芽孢杆菌Bacillussubtilis 168菌株所构建的工程菌GEB3产生的脂肽类抗生素种类。结果表明 ,GEB3仅产生表面活性素 (surfactin) 1种脂肽类抗生素。经LC MS分析 ,GEB3产生由 13、14和 15个碳原子的脂肪酸链构成的标准表面活性素变异体 (standardsurfactinisoforms)。生物活性检测表明 ,该工程菌产生的脂肽类抗生素表面活性素具有抑制小麦纹枯病菌和稻瘟病菌菌丝生长的作用     

鹿蹄草用于低盐酱菜保藏的研究

本实验对鹿蹄草应用于酱菜保藏的使用方法，条件和浓度进行了研究。实验结果表明，鹿蹄草对于导致酱菜败坏的酵母菌、细菌、霉菌均有较好的抑制效果，可使低盐酱菜在室温下的保质期延长２ｄ左右。抑菌圈实验表明，１００ｍｇ／Ｌ的鹿蹄草水煎液具有与同浓度的山梨酸钾和丙酸钙溶液相近或更好的抑菌效果。     

Additional notes to the checklist of Korean cultivated plants (5). Consolidated summary and indexes

Joint explorations have been carried out in North Korea for studying and collecting cultivated plants since 1985. A checklist of the cultivated plant species of the whole Korean peninsula was developed, used and updated since the beginning of the investigations. A last additional updating is provided including new entries, a summary and indexes (families, synonyms, vernacular names). The total number of taxa considered in the checklist, including infraspecific ones, is 608. On the species level it contains 581 entries belonging to 111 families and 381 genera. The number of medicinal plants is particularly high. The data are being maintained in a database. The results demonstrate the wealth of the Korean cultivated flora which is the result of a long history and a specific Korean input.     

Genetic and morphometric characterization of a local Vietnamese Swamp Buffalo population

The water buffalo plays a key role in the socio-economy of South-East Asia as it is the main draught power for paddy rice cultivation. While in the Indian subcontinent the water buffalo is the riverine type, in South-East Asia the majority of buffaloes are of the swamp type. In the poor remote northern province of Ha Giang in Vietnam, improvement of the swamp buffalo breed may be one of the best ways to increase sustainability of farming systems. Therefore, analysis of the genetic structure of the province buffalo population is a prerequisite to any conservation or improvement project. A total of 1122 animals were described for 11 body and horn measurements for morphometric characterization. From this sample set, 744 animals were genotyped for 17 microsatellite markers. Also 17 animals from southern provinces of Vietnam were genotyped as a comparative sample. The results showed that genetic diversity as well as inbreeding value in the Ha Giang was high. The F _ST values within the province and across Vietnam were low indicating that most of the population variation is explained by individual variability. Bayesian clustering analysis did not highlight the presence of subdivided populations. These results are useful for the implementation of a conservation and improvement strategy of the swamp buffalo in order to guarantee the householders' needs for sustainability of the farming system in the Ha Giang province.