全文获取类型
收费全文 | 2517篇 |
免费 | 189篇 |
专业分类
林业 | 294篇 |
农学 | 145篇 |
基础科学 | 8篇 |
504篇 | |
综合类 | 151篇 |
农作物 | 105篇 |
水产渔业 | 256篇 |
畜牧兽医 | 1015篇 |
园艺 | 30篇 |
植物保护 | 198篇 |
出版年
2023年 | 26篇 |
2022年 | 34篇 |
2021年 | 75篇 |
2020年 | 71篇 |
2019年 | 65篇 |
2018年 | 129篇 |
2017年 | 119篇 |
2016年 | 103篇 |
2015年 | 87篇 |
2014年 | 112篇 |
2013年 | 160篇 |
2012年 | 195篇 |
2011年 | 157篇 |
2010年 | 81篇 |
2009年 | 83篇 |
2008年 | 128篇 |
2007年 | 106篇 |
2006年 | 100篇 |
2005年 | 87篇 |
2004年 | 76篇 |
2003年 | 82篇 |
2002年 | 73篇 |
2001年 | 58篇 |
2000年 | 41篇 |
1999年 | 25篇 |
1998年 | 15篇 |
1997年 | 9篇 |
1996年 | 11篇 |
1994年 | 10篇 |
1992年 | 18篇 |
1991年 | 20篇 |
1990年 | 11篇 |
1989年 | 21篇 |
1988年 | 19篇 |
1987年 | 12篇 |
1985年 | 11篇 |
1984年 | 9篇 |
1983年 | 14篇 |
1979年 | 9篇 |
1978年 | 13篇 |
1977年 | 16篇 |
1975年 | 11篇 |
1974年 | 10篇 |
1973年 | 10篇 |
1972年 | 10篇 |
1971年 | 8篇 |
1969年 | 8篇 |
1967年 | 9篇 |
1966年 | 15篇 |
1921年 | 9篇 |
排序方式: 共有2706条查询结果,搜索用时 15 毫秒
91.
Major outer membrane proteins of Brucella spp.: past,present and future 总被引:16,自引:0,他引:16
The major outer membrane proteins (OMPs) of Brucella spp. were initially identified in the early 1980s and characterised as potential immunogenic and protective antigens. They were classified according to their apparent molecular mass as 36–38 kDa OMPs or group 2 porin proteins and 31–34 and 25–27 kDa OMPs which belong to the group 3 proteins. The genes encoding the group 2 porin proteins were identified in the late 1980s and consist of two genes, omp2a and omp2b, which are closely linked in the Brucella genome, and which share a great degree of identity (>85%). In the 1990s, two genes were identified coding for the group 3 proteins and were named omp25 and omp31. The predicted amino acid sequences of omp25 and omp31 share 34% identity. The recent release of the genome sequence of B. melitensis 16 M has revealed the presence of five additional gene products homologous to Omp25 and Omp31. The use of recombinant protein technology and monoclonal antibodies (MAbs) has shown that the major OMPs appear to be of little relevance as antigens in smooth (S) B. abortus or B. melitensis infections i.e. low or no protective activity in the mouse model of infection and low or no immunogenicity during host infection. However, group 3 proteins, in particular Omp31, appear as immunodominant antigen in the course of rough (R) B. ovis infection in rams and as important protective antigen in the B. ovis mouse model of infection. The major OMP genes display diversity and specific markers have been identified for Brucella species, biovars, and strains, including the recent marine mammal Brucella isolates for which new species names have been proposed. Recently, Omp25 has been shown to be involved in virulence of B. melitensis, B. abortus and B. ovis. Mutants lacking Omp25 are indeed attenuated in animal models of infection, and moreover provide levels of protection similar or better than currently used attenuated vaccine strain B. melitensis Rev.1. Therefore, these mutant strains appear interesting vaccine candidates for the future. The other group 3 proteins identified in the genome merit also further investigation related to the development of new vaccines. 相似文献
92.
Seguin BE Zhang TQ Buoen LC Weber AF Ruth GR 《Journal of the American Veterinary Medical Association》2000,216(1):65-67
OBJECTIVE: To determine prevalence of Holstein bulls with chromosomal anomalies, particularly the 1/21 centric fusion (CF), at a commercial artificial insemination (AI) company in the United States. DESIGN: Cross-sectional cytogenetic prevalence study. ANIMALS: All 606 Holstein bulls at a commercial AI company were cytogenetically screened to detect CF, chimerism, and other chromosomal abnormalities. PROCEDURE: Lymphocytes from heparinized blood samples were cultured by standard cytogenetic techniques, and chromosome spreads were prepared for microscopic examination. Chromosomal abnormalities were detected by examining 10 chromosome spreads per bull. Pedigree analysis was performed. RESULTS: None of the bulls had any type of CF. However, 6 bulls were identified as chimeras (i.e., contained lymphocytes with male [XY] and female [XX] chromosomes). One bull was sire or maternal grandsire to 85 of the bulls tested, and 739 of 1,212 (61%) sire and maternal-grandsire possibilities were accounted for by just 18 bulls. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of these results supports previous indications that CF is extremely rare in Holstein bloodlines available commercially via AI in the United States. However, chimeric bulls are more common, and they reportedly have decreased reproductive performance. Therefore, identification of chimeric sires in the AI facility reported here and the possibility of de novo onset of CF at any time indicates that early cytogenetic screening should be encouraged for prospective bulls intended for use in AI programs. 相似文献
93.
94.
Electro-acupuncture and Chinese herbs for treatment of cervical intervertebral disk disease in a dog
Ayne Murata Hayashi Júlia Maria Matera Tatiana Soares da Silva Ana Carolina Brand?o de Campos Fonseca Pinto Sílvia Renata Gaido Cortopassi 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):95-98
A non-ambulatory dog with tetraparesis following a pain episode that had evolved over 2 months was submitted for medical treatment and diagnosed with intervertebral disk disease at C3-C4 and dorsal extradural compression at C1-C2 and C3-C4 using myelography and computed tomography. The dog experienced ambulation recovery after 15 days of treatment with only electroacupuncture and Chinese herbal medicine, with marked improvement occurring after only 10 treatments. Six months of follow-up demonstrated that the dog was stable and had no recurrence of symptoms. Therefore, it was concluded that the combination of electroacupuncture and Chinese herbal medicine was responsible for motor rehabilitation. 相似文献
95.
Helena P. Trenado Isabel M. Fortes Diamantina Louro Jesús Navas-Castillo 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(2):193-196
Tomato chlorosis virus causes yellow leaf disorder epidemics in many countries worldwide. Plants of Physalis ixocarpa showing abnormal interveinal yellowing and plants of Physalis peruviana showing mild yellowing collected in the vicinity of tomato crops in Portugal were found naturally infected with ToCV. Physalis ixocarpa and P. peruviana were tested for susceptibility to ToCV by inoculation with Bemisia tabaci, Q biotype. Results confirmed that ToCV is readily transmissible to both species. The infection was expressed in P. ixocarpa by conspicuous interveinal yellow areas on leaves that developed into red or brown necrotic flecks, while P. peruviana test plants remained asymptomatic. Infected plants of both P. ixocarpa and P. peruviana served as ToCV sources for tomato infection via B. tabaci transmission. This is the first report of P. ixocarpa and P. peruviana as natural hosts of ToCV. 相似文献
96.
A.M. Prados-Ligero J.L. González-Andújar J.M. Melero-Vara M.J. Basallote-Ureba 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(9):861-870
Pseudothecia of Pleospora allii developed best on garlic leaf debris infected by Stemphylium vesicarium incubated at low temperature (5–10°C) and relative humidity (RH) close to saturation. RH of less than 96% prevented the formation of pseudothecia, while an incubation temperature of 15–20°C led to the early degeneration of pseudothecia. Under natural conditions, colonization by pseudothecia of unburied garlic leaf debris varied between seasons from 6.0 to 15.5 pseudothecia/mm2, whereas lower colonization levels were recorded when samples were buried. Pseudothecial maturity was reached 1–4mo after the deposition of garlic debris on the soil surface and 15 days after the burial of residues. In the later case, pseudothecia degenerated with degradation of the plant debris. Ascospore release, which required rainfall or dew periods, occurred between late January and late April depending upon the year. A high correlation was found between pseudothecia maturation and four meteorological variables. Two of which, i.e. the number of hours with RH98% and with a mean temperature of 4.5–10.5°C, and the accumulated rainfall, explained most variability (adjusted R2=0.82–0.98 depending upon the year). A multiple regression equation relating the pseudothecia maturity index with these two variables could be used to forecast the epidemic onset of Stemphylium leaf spots in Southern Spain. Temporal progress of pseudothecia maturation was best fitted by a monomolecular model. 相似文献
97.
F. Tanno U. Weber Ch. Wacker L. Gaschen V. Schmid J. Lang 《The Journal of small animal practice》1998,39(9):432-436
Ultrasonography was used to compare adhesions induced by two different methods of gastropexy in 16 dogs. An incisional gastropexy technique was used in eight dogs (group 1) and a 'modified' gastropexy technique in the remaining eight (group 2). The length and thickness of the gastropexy and the peristaltic activity of the stomach were measured ultrasonographically and compared between groups. Measurements for the two groups were taken in the early postoperative interval (two to four days), intermediate postoperative interval (eight to 20 days) and late postoperative interval (57 to 79 days). Both techniques were equally successful in forming permanent adhesions at two months postoperatively and there was no recurrence of gastric dilatation and volvulus. The length and thickness of the gastropexy were similar for both groups at two months postoperatively and there were no surgical complications with either technique. The modified gastropexy provides a technique that can easily be performed by a single surgeon , with no increased operative time or duration of anaesthesia, and with the formation of a permanent adhesion. 相似文献
98.
ULTRASONOGRAPHIC DETECTION OF ADRENAL GLAND TUMOR AND URETEROLITHIASIS IN A GUINEA PIG 总被引:1,自引:0,他引:1
Lorrie Gaschen DVM Cornelia Ketz Dr. med. vet. Johann Lang Dr. med. vet Urs Weber Dr. med. vet. L. Bacciarini Dr. med. vet. Ingrid Kohler med. vet. 《Veterinary radiology & ultrasound》1998,39(1):43-46
A 5-year-old guinea pig was presented to the University of Berne Small Animal Radiology Department for an ultrasound examination of the abdomen to confirm a suspected diagnosis of Cushing's syndrome. The patient had bilateral alopecia, was apathic and obese. Ultrasonographically, a tumor of the left adrenal gland, obstruction of the left ureter by an ureterolith, as well as hydronephrosis of the left kidney were detected. During surgery to relieve the ureteral obstruction the adrenal gland tumor was removed. The guinea pig died post-operatively due to blood loss. The left adrenal gland tumor was found histopathologically to be an adenoma and the right adrenal gland also had multiple small adenomas, but grossly appeared normal. The ureterolith was analyzed and found by x-ray diffraction to consist of calcium carbonate. 相似文献
99.
To determine if flower nutrient composition can be used to predict fruit quality, a field experiment was conducted over three seasons (1996-1999) in a commercial orange orchard (Citrus sinensis (L.) Osbeck cv. 'Valencia Late', budded on Troyer citrange rootstock) established on a calcareous soil in southern Portugal. Flowers were collected from 20 trees during full bloom in April and their nutrient composition determined, and fruits were harvested the following March and their quality evaluated. Patterns of covariation in flower nutrient concentrations and in fruit quality variables were evaluated by principal component analysis. Regression models relating fruit quality variables to flower nutrient composition were developed by stepwise selection procedures. The predictive power of the regression models was evaluated with an independent data set. Nutrient composition of flowers at full bloom could be used to predict the fruit quality variables fresh fruit mass and maturation index in the following year. Magnesium, Ca and Zn concentrations measured in flowers were related to fruit fresh mass estimations and N, P, Mg and Fe concentrations were related to fruit maturation index. We also established reference values for the nutrient composition of flowers based on measurements made in trees that produced large (> 76 mm in diameter) fruit. 相似文献
100.
T. Weber 《Journal of pest science》1964,37(4):59-60
Ohne Zusammenfassung 相似文献