全文获取类型
收费全文 | 415篇 |
免费 | 15篇 |
国内免费 | 2篇 |
专业分类
林业 | 63篇 |
农学 | 25篇 |
基础科学 | 1篇 |
19篇 | |
综合类 | 7篇 |
农作物 | 10篇 |
水产渔业 | 49篇 |
畜牧兽医 | 201篇 |
园艺 | 8篇 |
植物保护 | 49篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 25篇 |
2020年 | 6篇 |
2019年 | 5篇 |
2018年 | 10篇 |
2017年 | 9篇 |
2016年 | 9篇 |
2015年 | 21篇 |
2014年 | 16篇 |
2013年 | 15篇 |
2012年 | 17篇 |
2011年 | 27篇 |
2010年 | 19篇 |
2009年 | 22篇 |
2008年 | 34篇 |
2007年 | 28篇 |
2006年 | 40篇 |
2005年 | 28篇 |
2004年 | 20篇 |
2003年 | 23篇 |
2002年 | 15篇 |
2001年 | 7篇 |
2000年 | 10篇 |
1999年 | 2篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1996年 | 4篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1989年 | 1篇 |
1984年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有432条查询结果,搜索用时 31 毫秒
61.
Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method 下载免费PDF全文
Tao Zhuang Megumi Urakawa Hidetoshi Sato Yuko Sato Teruaki Taguchi Tsuyoshi Umino Shiro Katto Koutaro Tanaka Kozue Yoshimura Naokazu Takada Hiroko Kobayashi Megumi Ito Michael T. Rose Yoshio Kiku Yuya Nagasawa Haruki Kitazawa Kouichi Watanabe Tomonori Nochi Tomohito Hayashi Hisashi Aso 《Animal Science Journal》2018,89(7):1011-1019
Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a+/CD11c+/MHC (major histocompatibility complex) class II+ cells. Although DCs are identified at 0.1%–0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic‐activated cell sorting, and purified the CD172a+/CD11c+ DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co‐stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co‐stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage. 相似文献
62.
Chahan B Jian Z Jilintai Miyahara K Tanabe S Xuan X Sato Y Moritomo T Nogami S Mikami T Maruyama S Inokuma H 《Veterinary parasitology》2007,144(1-2):184-187
Tick DNA samples from cattle in Xinjiang Uygur Autonomous Region Area, China, were examined for Rickettsia infection by citrate synthase gene-based PCR and sequencing. Four positive samples were detected from Haemaphysalis danieli and high levels of similarity were found with recently detected 'Candidatus Rickettsia principis.' 相似文献
63.
Comparison and phylogenetic analysis of the heat shock protein 70 gene of Babesia parasites from dogs 总被引:1,自引:0,他引:1
Yamasaki M Inokuma H Sugimoto C Shaw SE Aktas M Yabsley MJ Yamato O Maede Y 《Veterinary parasitology》2007,145(3-4):217-227
The heat shock protein 70 (hsp70) genes of Babesia gibsoni, B. canis canis, B. canis vogeli, and B. canis rossi isolated from infected dogs were cloned by polymerase chain reaction (PCR) and sequenced. In the nucleotide sequence and the predicted amino acid sequence of the gene, the parasites were very similar to each other. The nucleotide sequences of the hsp70 gene had more variety than those of 18S nuclear subunit ribosomal DNA (18S rDNA). A phylogenetic analysis of these sequences and comparisons with sequences from other Babesia and Theileria species revealed that all canine babesial isolates analyzed in the present study were closely related to each other and formed one cluster. Additionally, a phylogenetic analysis of Babesia and Theileria species showed that these parasites could be divided into three groups: group A including canine babesial isolates, B. divergens, B. odocoilei, B. bovis, B. caballi, and B. ovis; group B including Theileria annulata, T. orientalis, and T. cervi; and group C including B. microti and B. rodhaini. These results suggested that a phylogenetic analysis of the hsp70 gene sequence might be helpful in classifying Babesia and Theileria species, and that canine babesial isolates might be closely related to each other, indicating their evolution from the same ancestry. 相似文献
64.
Tamamoto C Seino N Suzuki M Kaji K Takahashi H Inokuma H 《Veterinary parasitology》2007,150(4):370-373
Ehrlichia muris DNA was detected in the blood of sika deer (Cervus nippon yesoensis) by species-specific PCR based on the citrate synthase gene, which was shown to be more sensitive than species-specific PCR based on the 16S rRNA gene. Among 102 deer examined, one deer was positive. Deer may be a possible mammalian reservoir of E. muris. 相似文献
65.
Allelic variation in high-molecular-weight glutenin subunit Loci of Glu-1 in Japanese common wheats 总被引:2,自引:0,他引:2
Seed storage proteins of 131 Japanese Norin wheat (Triticum aestivum) varieties were fractionated by sodium dodecyl sulfate
polyacrylamide gel electrophoresis to determine allelic make-up in varieties at each of three loci that control high-molecular-weight
(HMW) glutenin subunits. Three alleles were identified at the Glu-A1 locus, six at the Glu-B1 locus and five at the Glu-D1
locus. Twenty-four different, major glutenin HMW subunits were identified and each contained three to five subunits and seventeen
different glutenin subunit patterns were observed for 19 subunits in the 131 Japanese Norin varieties. Fourteen alleles were
identified by comparison of subunit mobility with that previously found in hexaploid wheat. Japanese Norin varieties showed
a specific pattern of allelic variation in glutenin HMW subunits, different from that of Chinese and other country common
wheats in allelic frequency at Glu-1 loci.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
66.
Hisashi Nishiwaki Yoshiaki Nakagawa David Y Takeda Atsushi Okazawa Miki Akamatsu Hisashi Miyagawa Tamio Ueno Keiichiro Nishimura 《Pest management science》2000,56(10):875-881
Variously substituted benzyl derivatives of chloronicotinyl insecticides were synthesized with a wide range of substituents including halogens, NO2, CN, CF3 and small alkyl and alkoxy groups at the ortho, meta and para positions, as well as multiple‐substituted benzyl analogues. Their binding activity to the α‐bungarotoxin binding site in housefly (Musca domestica) head membrane preparations was measured. Among the compounds tested, the activity of the meta‐CN derivative was the highest, being 20–100 times higher than those of imidacloprid, acetamiprid and nitenpyram. The synergized insecticidal activity against houseflies was also measured for selected compounds with the metabolic inhibitor, NIA16388 (propargyl propyl phenylphosphonate). For the nitromethylene analogues, including both benzyl and pyridylmethyl analogues, higher binding activity usually resulted in higher insecticidal activity. © 2000 Society of Chemical Industry 相似文献
67.
Yutaro Sakai Nobuyuki Yagi Masahiko Ariji Atsushi Takahara Hisashi Kurokura 《Fisheries Science》2009,75(5):1079-1087
A demand system analysis was conducted to examine the substitute relationships between tuna and skipjack tuna in the Japanese
market. Data from the Annual Report on Family Income and Expenditure Survey from 1965 to 2006 were used for the analysis using
the almost ideal demand system (AIDS). Results suggest that skipjack tuna can be a strong substitute for tuna, while other
fish groups are not a clear substitute. Our analysis of substitute relationships among fish species in a market indicates
that this is a factor that should be considered for better fisheries resource management. For instance, even under a situation
where one fish species is underexploited, proper attention to its fishery management is necessary if the fish is a strong
substitute for another popular fish species in the market. 相似文献
68.
Atsushi Okazawa Miki Akamatsu Akira Ohoka Hisashi Nishiwaki Won-Jea Cho Yoshiaki Nakagawa Keiichiro Nishimura Tamio Ueno 《Pest management science》1998,54(2):134-144
The binding activity of imidacloprid and related compounds to nicotinic acetylcholine receptors (nAChR) of house flies was measured by use of radioactive α-bungarotoxin as a ligand. Variations in the activity were examined three-dimensionally using comparative molecular field analysis (CoMFA). The CoMFA results suggest that one conformer among the four stable ones is active and provide support for one of the proposed binding models for this class of compound, in which the nitrogen atom of the pyridine ring and the nitrogen atom at the 1-position of the imidazolidine ring interact with the hydrogen-donating and electron-rich sites of nAChR, respectively. The CoMFA field map showed that the nitroimino moiety and a portion of the imidazolidine ring were mainly surrounded by a sterically and electrostatically sensitive region of nAChR. © 1998 Society of Chemical Industry 相似文献
69.
Relationship Between Allelopathic Effects and Functional Traits of Different Allelopathic Potential Rice Accessions at Different Growth Stages 总被引:1,自引:1,他引:0
Xu Gaofeng Shen Shicai Zhang Fudou Zhang Yun Kato-Noguchi Hisashi Roy Clements David 《水稻科学》2018,25(1):32-41
In this study,effects of temperature,light and their interactions on allelopathic effects and the functional traits specific leaf area(SLA)and stem mass fraction(SMF)of different allelopathic potential rice accessions at different growth stages were analyzed.The main results were as follows:Allelopathic responses to temperature and light varied with different allelopathic potential rice accessions at different growth stages.With the rise of temperature and the extension of photoperiod,allelopathic effect increased firstly and then decreased at 2–3 leaf stage,but increased constantly at the 4–5 and 7–8 leaf stages in strong allelopathic rice accessions[O.longistaminata,F1(O.longistaminata×RD23),F2(RL159 and RL169)].Temperature had significant impact on allelopathic effect without considering light factors,but light showed little effect on rice allelopathy at the same temperature conditions.The greatest allelopathic effect was attained with moderate temperature and long photoperiod at 2–3 leaf stage in strong allelopathic rice accessions,but all the rice accessions showed weak allelopathic effects at the low temperature condition(15oC/10oC),and the influence of different factors on allelopathy followed a general trend as temperatureleaf stagelight,indicating that among the multiple factors impacting rice allelopathy,temperature was the main factor.Allelopathic characteristics of F1 and F2 to various temperature and light were similar to O.longistaminata,showing that allelopathic genes from wild rice can be expressed in its descendants.Temperature and light also had significant effects on SLA and SMF,and rice allelopathy was closely correlative to SLA in strong allelopathic rice accessions at the 4–5 and 7–8 leaf stages,but there was no correlation between rice allelopathy and SMF at different growth stages.These results suggested that rice adjust the relationship between allelopathy and SLA and adapt to the varied environments,and that high temperature and long photoperiod can enhance rice allelopathic activity. 相似文献
70.