全文获取类型
收费全文 | 877篇 |
免费 | 36篇 |
专业分类
林业 | 120篇 |
农学 | 45篇 |
128篇 | |
综合类 | 26篇 |
农作物 | 35篇 |
水产渔业 | 88篇 |
畜牧兽医 | 390篇 |
园艺 | 5篇 |
植物保护 | 76篇 |
出版年
2023年 | 2篇 |
2022年 | 13篇 |
2021年 | 25篇 |
2020年 | 19篇 |
2019年 | 11篇 |
2018年 | 15篇 |
2017年 | 22篇 |
2016年 | 33篇 |
2015年 | 39篇 |
2014年 | 44篇 |
2013年 | 77篇 |
2012年 | 56篇 |
2011年 | 64篇 |
2010年 | 32篇 |
2009年 | 42篇 |
2008年 | 61篇 |
2007年 | 51篇 |
2006年 | 70篇 |
2005年 | 55篇 |
2004年 | 56篇 |
2003年 | 56篇 |
2002年 | 33篇 |
2001年 | 10篇 |
2000年 | 10篇 |
1999年 | 5篇 |
1998年 | 7篇 |
1996年 | 1篇 |
1991年 | 1篇 |
1988年 | 2篇 |
1983年 | 1篇 |
排序方式: 共有913条查询结果,搜索用时 15 毫秒
221.
222.
Hiroyuki Satoh Osamu Yamato Tomoya Asano Masahiro Yamasaki Yoshimitsu Maede 《Journal of veterinary diagnostic investigation》2004,16(3):223-226
GM1- and GM2-gangliosidoses are lethal lysosomal diseases that are caused by a defect of acid hydrolases, resulting in the intralysosomal accumulation of the specific physiological substrates, GM1- and GM2-gangliosides, respectively. In the present study a method for the diagnosis of canine GM1-gangliosidosis was established using canine cerebrospinal fluid (CSF). The concentration of GM1-ganglioside in CSF was determined by thin-layer chromatography-enzyme immunostaining using biotin-conjugated cholera toxin B, which specifically binds with GM1-ganglioside. The concentration of CSF GM1-ganglioside was increased in Shiba dogs with GM1-gangliosidosis, and the increased level was approximately proportional to the age of the dogs. The concentration was high in the affected dog even at 5 months of age, when Shiba dogs with GM1-gangliosidosis first manifest neurologic signs. In addition, the concentration of CSF GM1-ganglioside in a dog with the GM2-gangliosidosis 0 variant (Sandhoff disease) was also 7 times the normal level. From these results it was concluded that this laboratory technique enables a definitive and early diagnosis of canine GM1-gangliosidosis even if tissues and organs cannot be obtained. However, because GM1-ganglioside can also be elevated in cases of GM2-gangliosidosis, it is necessary to assay for specific enzyme deficiencies to definitively separate GM1- from GM2-gangliosidosis. 相似文献
223.
Osamu Yamato Asogi Kobayashi Hiroyuki Satoh Daiji Endoh Toru Shoda Yukiko Masuoka Ayano Hatakeyama Eun-Og Jo Tomoya Asano Madoka Yonemura Masahiro Yamasaki Yoshimitsu Maede 《Journal of veterinary diagnostic investigation》2004,16(4):299-304
In the present study, diagnostic methods for canine G(M1)-gangliosidosis were examined by comparing a DNA mutation assay with an enzyme assay. Sixty-two Shiba dogs of a pedigree with G(M1)-gangliosidosis were differentiated into 3 genotypes, i.e., normal, heterozygous, and homozygous affected dogs, using a DNA mutation assay, which consists of polymerase chain reaction amplification and the determination of restriction fragment length polymorphisms. The beta-galactosidase activity in leukocytes, umbilical cords, and plasma was measured using 4-methylumbelliferyl beta-D-galactoside and p-nitrophenyl beta-D-galactoside as artificial substrates and compared among the 3 genotypes. The results showed that it was possible to identify homozygous dogs with the enzyme assay using leukocytes and umbilical cords. When using leukocytes, heterozygous carriers could be differentiated from normal dogs in many cases. However, the use of the DNA mutation assay is essential for a complete determination of heterozygous carriers because of the overlap in the distribution of enzyme activity between these 2 groups. When umbilical cords were used, heterozygous carriers could not be differentiated from normal dogs because of no significant difference in enzyme activity between these 2 groups. The beta-galactosidase activity in plasma was not applicable to the diagnosis and genotyping of G(M1)-gangliosidosis in Shiba dogs. 相似文献
224.
OBJECTIVE: To report the clinical findings, surgical technique, and outcome after repair of urinary bladder rupture through a urethral incision in postpartum mares. STUDY DESIGN: Retrospective study. ANIMALS: Four Thoroughbred broodmares. METHODS: Medical records were reviewed for clinical signs, surgical technique, medical therapy, and outcome. The bladder was everted into the vagina through a urethral incision that included a sphincterotomy. The bladder defect was repaired with absorbable suture material in a single-layer, full thickness, simple, continuous pattern. The urethral incision was closed similarly. RESULTS: Depression, inappetence, signs of shock, dehydration, azotemia, and serum electrolyte abnormalities were consistent findings that increased temporally after bladder rupture. Each bladder defect was repaired successfully, and metabolic derangements were corrected with supportive medical therapy. All mares survived, conceived, and had more foals without further complications CONCLUSION AND CLINICAL RELEVANCE: Bladder rupture associated with parturition in mares can be repaired in a standing position by eversion of the bladder through a urethrotomy and urethral sphincterotomy. 相似文献
225.
Serum leptin levels during the periparturient period in cows 总被引:1,自引:0,他引:1
Soliman M Ishioka K Yoshida R Komabayashi K Hatai H Matsui Y Hirai T Katagiri S Takahashi Y Kawakita Y Abe H Kitamura H Kimura K Saito M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1053-1056
Serum leptin concentrations were measured in antenatal and postnatal cows housed at two different locations. The mean serum leptin concentration was 9.2 +/- 0.6 ng/m l (n=22) in one group, and was slightly lower in the other (7.4 +/- 0.4 ng/ml, n=54), probably because of the different nutritional conditions between the two groups. There was no consistent variation in relation to the menstrual cycle and the periparturient period in both groups. Moreover, serum leptin concentrations during the periparturient period were independent of the number of delivery and the incidence of mastitis and milk fever. These results are quite different from those in rodents and human, suggesting the different regulatory mechanism of circulating leptin concentration in cows. 相似文献
226.
Nagahata H Oota H Nitanai A Oikawa S Higuchi H Nakade T Kurosawa T Morita M Ogawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1107-1112
A female stillborn Holstein calf with shortened cervical and thoracic regions, protrusion of the tongue, and bilateral symmetric flexural contraction of the anterior limbs was delivered on gestation day 281. Multiple hemivertebrae, fused and misshaped vertebrae, synostosis and scoliosis of cervical, thoracic and lumbar vertebral column were found in the affected calf by radiographic and computed tomographic (CT) analysis. Ten pairs of ribs were present and the sternum consisted of 9 sternebrae. Multiple morphologic abnormalities including fusion, malformation, and displacement, were found in the ribs and sternum. Cardiac anomalies, including atrial septal defect and hypertrophy of right ventricle, were observed. DNA-polymerase chain reaction (PCR) analysis demonstrated that amplified product from the liver DNA of the affected calf had identical pattern to that associated with complex vertebral malformation (CVM) of Holstein calves and that her dam was a heterozygous carrier of CVM. The affected calf was diagnosed as having CVM based on the DNA-PCR results and the characteristic findings, and was recorded as a first documentation of CVM confirmed in a Holstein calf in Japan. 相似文献
227.
Hiroyuki Takahara Kazuhiro Toyoda Gento Tsuji Yasuyuki Kubo Yoshishige Inagaki Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2005,71(3):190-195
Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection. 相似文献
228.
Pongphen Jitareerat Hiroyuki Matsumoto Masahiro Umehara Shinji Tsuyumu 《Journal of General Plant Pathology》2003,69(1):49-54
Erwinia chrysanthemi (Ech) triggers soft rot disease mainly by secreting pectate lyase (Pel), which is regulated in a complex manner by many regulatory
genes. In a previous study, we used a gene dosage method to show that the ddl gene, which encodes d-alanine-D-alanine ligase, reduced Pel production and tissue maceration by Ech strain EC16n. In this study, the ddl
− marker-exchanged mutant was shown to overcome the long growth lag caused by various salts in the growth medium and to increase
Pel production over that by EC16n, especially in a medium containing magnesium salts. Thus, ddl seems to regulate Pel production in a negative manner. Because the profiles of a gel shift assay using the pelE promoter region as the target DNA with crude extracts of EC16n and ddl
− mutant were distinguishable, Ddl is thought to affect the binding of other regulatory proteins. Expression of the ddl gene was induced in the medium containing a low-molecular-weight fraction of potato extract, but it was reduced in that containing
both polygalacturonic acid (PGA) and the fraction. The repression of ddl expression by PGA should contribute in part to the in planta hyperinduction of Pel.
Received: May 15, 2002 / Accepted: June 20, 2002 相似文献
229.
Takashi Kobayashi Eiji Kanda Shigeo Naito Toshihiko Nakajima Ichiro Arakawa Kazutoshi Nemoto Masanao Honma Hiroyuki Toujyou Kiyoshi Ishiguro Katsuki Kitada Yoichi Torigoe 《Journal of General Plant Pathology》2003,69(1):17-22
Rice reflectance was measured to determine the spectral regions most sensitive to leaf blast infection with a multispectral
radiometer. As disease severity increased, reflectance also increased in the 400–500 nm (blue), 570–700 nm (red), and 900–2000 nm
regions but decreased in the 500–570 nm and 700–900 nm regions. The increased reflectance in the blue and red regions may
be attributed to decreased chlorophyll and carotenoid contents in response to the blast infection. The maximum and minimum
reflectance differences occurred at 680 nm and 760 nm for the nondiseased and diseased rice, respectively. The spectral location
of maximum sensitivity was 675 nm regardless of disease severity. Rice reflectance ratios were evaluated as indicators of
leaf blast severity. Two ratios, R550/R675 (reflectance at 550 nm divided by reflectance at 675 nm), and R570/R675 quantified
the significant disease severity. These wavelengths were selected based on the sensitivity minima and maxima. The ratios of
nondiseased rice plants varied depending on growth stage. The variation in ratios must be considered when they are used to
estimate leaf blast severity.
Received: April 2, 2002 / Accepted: August 12, 2002 相似文献
230.
Naruto FURUYA Hiroyuki URA Kazuhiro IIYAMA Masaru MATSUMOTO Minoru TAKESHITA Yoichi TAKANAMI 《Journal of General Plant Pathology》2002,68(3):220-224
Specific primers were designed based on the sequences of the spacer region between the 16S and 23S ribosomal DNA (rDNA) for
direct, rapid and specific detection of Burkholderia gladioli. These primers were named GLA-f and GLA-r. PCR performed on boiled bacterial suspensions yielded an amplification product of
approximately 300 bp. No products from other bacterial species, including B. glumae were amplified, even after complete DNA extraction by the cetyltrimethyl-ammonium bromide (CTAB) method. Using the specific
primers designed in this study, the PCR method can detect B. gladioli in plant samples within 6 hr. These data demonstrate the potential of specific PCR for the detection of B. gladioli.
Received 10 December 2001/ Accepted in revised form 15 April 2002 相似文献