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21.
To clarify mechanisms underlying variation in transpiration rate among deciduous broad-leaved tree species, we measured diurnal changes in stomatal conductance (gs) and leaf water potential, and calculated the maximum transpiration rate (Emax), leaf-specific hydraulic conductance (K(s-l)) and difference between the soil water potential and the daily minimum leaf water potential (Psis - Psi(l,min)). Pressure-volume (P-V) measurements were made on leaves. Saplings of eight broad-leaved tree species that are common in Japanese cool temperate forests were studied. Maximum transpiration rate varied significantly among species. There was a statistically significant difference in Psis - Psi(l,min), but not in K(s-l). Species with large Emax also had large Psis - Psi(l,min) and gs. The results of the P-V analyses showed that species with a large Psis - Psi(l,min) maintained turgor even at low leaf water potentials. The similar daily minimum leaf pressure potentials (Psip) across all eight species indicate that Psip values below this minimum are critical. Based on these results, we suggest that the leaf cell capacity for turgor maintenance strongly affects Psis - Psi(l,min) and consequently Emax via stomatal regulation. 相似文献
22.
Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
Masatoshi OOGA Masataka G. SUZUKI Fugaku AOKI 《The Journal of reproduction and development》2015,61(3):179-184
Histone H2B monoubiquitination (H2Bub1) plays an important role in developmental regulation in various vertebrate species. However, the role of H2Bub1 in mammalian preimplantation development remains unclear. In the present study, we examined the role of H2Bub1 in the regulation of mouse preimplantation development. Based on immunocytochemical analysis using an anti-H2Bub1 antibody, no H2Bub1 signal was detected in the metaphase chromosomes of unfertilized oocytes or the pronuclei of early 1-cell stage embryos, but a weak signal was observed in late 1-cell stage embryos. The signal increased after cleavage into the 2-cell stage, and thereafter a strong signal was observed until the blastocyst stage. To assess the significance of H2Bub1 in the regulation of preimplantation development, RNF20 (an H2B-specific ubiquitin E3 ligase) was knocked down using small interfering RNA (siRNAs). In embryos treated with siRNA, the levels of Rnf20 mRNA and H2Bub1 decreased
at the 4-cell and morula stages. Although these embryos developed normally until the morula stage, only one-third developed into the blastocyst stage. These results suggested that H2Bub1 is involved in the regulation of preimplantation development. 相似文献
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ABSTRACT: Life history parameters (growth pattern, the length of the spawningseason and age at sexual maturity) of Apogon lineatus wereinvestigated from three localities in Japan. The maximum ages werefound to be 3 years in Tokyo Bay, 2 years in Osaka Bay and 5 yearsoff Niigata Prefecture. Both sexes from Tokyo Bay grew to largersizes between the ages of 1 and 2 years than both sexes from otherlocalities, food availability being suggested as the major factorfor differences in growth. Spawning seasons were during 4 months(July–October) in Tokyo Bay and Osaka Bay, but were foundto only occur during the two months of July and August off NiigataPrefecture. Factors that could limit the spawning season off NiigataPrefecture were not determined. Age at maturity for both sexes inall localities appeared to be 1 year. We could not elucidate whetherthese variations in life history parameters arose from genetic differencesor from phenotypic plasticity. In either case, the trade-off betweenthe maximum age and the length of the spawning season in the offNiigata Prefecture population and any other population may be interpretedas an adaptation to local conditions, such as with the off NiigataPrefecture population. 相似文献
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Kittaka-Katsura H Fujita T Watanabe F Nakano Y 《Journal of agricultural and food chemistry》2002,50(17):4994-4997
Vitamin B(12) content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9-211.6 microg/100 g dry weight determined by the chemiluminescence method were similar to the values (201.3-285.7 microg/100 g dry weight) determined by the microbiological method. A corrinoid compound was purified to homogeneity from the Chlorella tablets and characterized. The purified corrinoid compound was identified as vitamin B12, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-Vis spectroscopy. 相似文献
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Expression and function of the segmentation gene fushi tarazu during Drosophila neurogenesis 总被引:33,自引:0,他引:33
Segmentation genes control cell identities during early pattern formation in Drosophila. One of these genes, fushi tarazu (ftz), is now shown also to control cell fate during neurogenesis. Early in development, ftz is expressed in a striped pattern at the blastoderm stage. Later, it is transiently expressed in a specific subset of neuronal precursor cells, neurons (such as aCC, pCC, RP1, and RP2), and glia in the developing central nervous system (CNS). The function of ftz in the CNS was determined by creating ftz mutant embryos that express ftz in the blastoderm stripes but not in the CNS. In the absence of ftz CNS expression, some neurons appear normal (for example, the aCC, pCC, and RP1), whereas the RP2 neuron extends its growth cone along an abnormal pathway, mimicking its sibling (RP1), suggesting a transformation in neuronal identity. 相似文献
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Oscillatory expression of the bHLH factor Hes1 regulated by a negative feedback loop 总被引:1,自引:0,他引:1
Hirata H Yoshiura S Ohtsuka T Bessho Y Harada T Yoshikawa K Kageyama R 《Science (New York, N.Y.)》2002,298(5594):840-843
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