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591.
To clarify the genetic mechanism which controls the variation in amylose content among nonwaxy landraces of foxtail millet,
the inheritance of different starch types in endosperm was examined by I2-KI staining. The level of starch granule bound protein in foxtail millet endosperm was also analyzed using SDS-PAGE. The
segregation for starch types in F2 and F3 seeds determined by I2-KI staining showed that there are three different alleles at the waxy (wx) locus. A major protein bound to starch granules
was detected in nonwaxy endosperm but it was absent in most of the waxy endosperm, suggesting that the protein is the Wx gene
product which is responsible for the synthesis of amylose in endosperm. The level of Wx protein proportionally corresponded
to the amylose content among nonwaxy landraces. This implies that two different Wx alleles regulate quantitative levels of
Wx protein. Landraces with the allele for low amylose content are from Taiwan, the Philippines, Indonesia and Thailand.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
592.
Hiroki Inoue Shigehiro Kamoda Tamami Terada Yoshimasa Saburi 《Journal of Wood Science》1998,44(5):375-378
The contents of diterpenoids, ginkgolides A, B, and C, in seeds, embryos, and plantlets ofGinkgo biloba were analyzed to clarify the relations between organogenesis and terpene contents inG. biloba. There is so far no published report on the contents and changes in such terpenes in seeds and very young plantlets ofG. biloba. Ginkgolides were present in seeds and embryos. Plantlets cultured in both the dark and under illumination contained substantial amounts of ginkgolides, more abundant than in seeds and embryos. It is concluded that ginkgo yields ginkgolides in its early stage of development regardless of light. 相似文献
593.
594.
Yingjuan Liu Chunyun Zhang Jinliang Du Rui Jia Liping Cao Galina Jeney Hiroki Teraoka Pao Xu Guojun Yin 《Fish physiology and biochemistry》2017,43(5):1209-1221
The aim of the present study was to investigate the protective effects of Ganoderma lucidum polysaccharide (GLPS) against carbon tetrachloride (CCl4)-induced hepatotoxicity in vitro in common carp. Precision-cut liver slices (PCLSs), which closely resemble the organ from which they are derived, were employed as an in vitro model system. GLPS (0.1, 0.3, and 0.6 mg/ml) was added to PCLS culture system before the exposure to 12 mM CCl4. The supernatants and slices were collected to detect molecular and biochemical responses to CCl4 and PCLS treatments. The levels of CYP1A, CYP3A, and CYP2E1 were measured by ELISA; the mRNA expressions of TNF-α, IL-1β, IL-6, and iNOS were determined by RT-PCR; and the relative protein expressions of c-Rel and p65 were analyzed by western blotting. Results showed that GLPS inhibited the elevations of the marker enzymes (GOT, GPT, LDH) and MDA induced by CCl4; it also enhanced the suppressed activity of antioxidant enzymes (SOD, CAT, GSH-Px, T-AOC). The treatment with GLPS resulted in significant downregulation of NF-κB and inflammatory cytokine mRNA levels and significant decreases in the hepatic protein levels of CYP1A, CYP3A, and CYP2E1. These results suggest that GLPS can protect CCl4-induced PCLS injury through inhibiting lipid peroxidation, elevating antioxidant enzyme activity, and suppressing immune inflammatory response. 相似文献
595.
596.
Shigeru Katayama Toshihiro Nishio Zensuke Iseya Hideki Kishimura Hiroki Saeki 《Fisheries Science》2009,75(2):491-497
A highly viscous polysaccharide solution was extracted from Gagome Kjellmaniella crassifolia in 20°C water. The eluted sugar concentration was 0.16%, and the extracted carbohydrates consisted of fucoidan, laminaran,
and alginate at an approximately ratio of 8.2:0.8:1.0. An increase in the extraction temperature resulted in a less viscous
solution, even though the amount of eluted sugar was higher than that obtained at a lower temperature. The most viscous extraction
solution was obtained at a neutral pH, with a more acidic or alkaline extraction solution being a less viscous. When the
highly viscous polysaccharide solution was heated, the viscosity decreased markedly with increasing temperature. The viscosity
of the polysaccharide solution increased after dialysis against water and decreased with the addition of either KCl or NaCl.
However, the viscosity was recovered to previous levels by following re-dialysis against water. The removal of divalent cations
by EDTA and the re-addition using CaCl2 or MgCl2 also caused reversible changes to the viscosity. These characterizations will be useful for widespread applications of viscous
K. crassifolia polysaccharides. 相似文献
597.
Keitaro Tanoi Junko Hojo Kazutoshi Suzuki Yoshitake Hayashi Hiroki Nishiyama Tomoko M. Nakanishi 《Soil Science and Plant Nutrition》2005,51(5):715-717
We investigate the effect of Al on K+ uptake by rice roots. Potassium-38 (38 K), a positron emitting nuclide (the half-life: 7.61 min), was used to trace K+ behavior. When a rice root was treated with 10μM Al for 24 h, the uptake of 38 K in the root was increased in the range of 1 to 2 cm from the root tip compared with that of the control sample. Because the root continued to grow without showing any damage of plasma membrane during the Al treatment, it was suggested that the 38 K uptake was not occurred through diffusion into the cells. The uptake of 38 K in both treatments, with/without Al, was decreased by VO4 3- (inhibitor of H+ -ATPase on plasma membrane) and DNP (H+ ionophore) treatment, which suggested that the K+ uptake was performed through an active transport, such as H+ :K+ transport or H+ gradient promoted by an Al treatment. 相似文献
598.
Takashi OKURA Hiroki OTOMO Shoko SUZUKI Yuji ONO Akira TANENO Eiji OISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1686
Infectious bursal disease (IBD) causes severe economic damage to the poultry industry worldwide. To prevent IBD virus (IBDV) infection, live virus vaccines have been widely used in chickens having wide-ranging levels of maternally derived antibodies. But, the risks of infection with other pathogens because of lesions related to atrophy of the bursa of Fabricius in vaccinated chickens are a concern. To resolve the problems, a recombinant turkey herpesvirus (HVT) vaccine expressing IBDV-VP2 protein (rHVT-IBD) has been developed. However, the induction of neutralizing antibodies by rHVT-IBD against a virulent IBDV might be delayed compared with that by the live IBD vaccine, leading to the high risks of IBDV infection for young chickens. To find the best selection of IBDV vaccine for the onset of immunity, we examine the protective efficacy of a novel in ovo-attenuated live IBDV (IBD-CA) vaccine and the rHVT-IBD vaccine in young chickens challenged with a very virulent IBDV (vvIBDV) strain. We show that the protective efficacy of IBD-CA vaccine was higher than that of the rHVT-IBD vaccine in 14-day-old chickens challenged with the vvIBDV strain, leading to the risk of IBDV infection for young chickens when vaccinated with rHVT-IBD. Our results suggest that farmers should select the best vaccines to maximize vaccine efficacy in consideration of the vaccine characteristics, prevalence levels of IBDV in the areas, and initial MDA levels of the chickens since the attenuated live and recombinant vaccines play a role in the different vaccine efficacies. 相似文献
599.
Erika KAWASAKI Makoto HASEBE Ji-Hee HWANG Eun-Young KIM Kisup LEE Kunikazu MOMOSE Hiroki TERAOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):233
Red-crowned cranes Grus japonensis, which are an endangered species, have two separate populations, a mainland population in the Eurasian continent and an island population in eastern Hokkaido, Japan. Island cranes showed three haplotypes (Gj1, Gj2 and Gj13), whereas ten haplotypes (Gj3–Gj12) were confirmed in captive cranes and stray cranes. We found Gj5 haplotype in feathers of two cranes as well as four new haplotypes in seven wild crane feathers collected in South Korea. We also found feathers in the nest in Sarobetsu Wetland in northwestern Hokkaido. While the haplotype of female-derived feathers was Gj2, that of male-derived feathers was Gj5. The results suggest that there has been crossbreeding between cranes in the island population and cranes in the mainland population. 相似文献
600.
Eiji NAITO Kohei NAKATA Hiroki SAKAI Osamu YAMATO Md Shafiqul ISLAM Sadatoshi MAEDA Hiroaki KAMISHINA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):199
Canine degenerative myelopathy (DM) is a progressive neurodegenerative disease of the spinal cord. The diagnosis is based on the observation of clinical signs, genetic testing, and exclusion of other spinal cord diseases, and a definitive diagnosis of DM can only be confirmed by postmortem histopathological findings. The aim of this study was to investigate the diagnostic ability of diffusion tensor imaging (DTI) for DM. Eight DM-affected Pembroke Welsh Corgis, thirteen dogs with thoracolumbar intervertebral disk herniation (IVDH), and six healthy control dogs were included. All dogs were scanned using a 3.0-T MRI system. Apparent diffusion coefficient (ADC) and fractional anisotropy (FA) values were calculated for each intervertebral disk level slice between T8–T9 and L2–L3 intervertebral disk levels, and the entire area of the thoracolumbar spinal cord between T8–T9 and L2–L3 intervertebral disk levels (T8–L3 region). The ADC and FA values of the T8–L3 region were significantly lower in the DM group than in the IVDH group. The ADC values for the T8–L3 region had a moderate negative correlation with clinical duration (rs= −0.723, P=0.043); however, the FA values of other intervertebral disk levels and T8–L3 region had no correlation with clinical durations. The measurement of DTI indices can be used to quantitatively assess neurodegeneration and may have diagnostic value for DM. In particular, the ADC value of the T8–L3 region may aid in making a non-invasive premortem diagnosis of DM. 相似文献