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61.
Tsutomu Arie Yumiko Kobayashi Yoshiki Kono Okada Gen Isamu Yamaguchi 《Pest management science》1999,55(5):602-604
Phoma glomerata strain JCM9972 controls clubroot of cruciferous crops caused by Plasmodiophora brassicae and the activity depends upon epoxydon (5-hydroxy-3-(hydroxymethyl)-7-oxabicyclo [4.1.0]hept-3-en-2-one) produced by the strain. 相似文献
62.
To understand the genetic background of panicle characteristics in temperate japonica rice (Oryza sativa L.), we genetically analyzed DH lines derived from a cross between two temperate japonica rice cultivars, ‘Akihikari’ and
‘Koshihikari’,in 1996 and 1997. Four traits of panicle characteristics, number of primary branches per panicle (NPB), number
of secondary branches per panicle (NSB), average number of spikelets on one primary branch (NSP)and average number of spikelets
on one secondary branch (NSS), in 212 DH lines were measured, and the interval mapping of QTLs for these traits was carried
out using169 DNA markers with an LOD threshold of2.5. Five, three and one putative QTLs for NPB, NSB and NSS were identified,respectively,
and no QTLs relating to NSP appeared. The percentages in total phenotypic variation explained by all putative QTLs for NPB
were 35.5%: and43.8% in 1996 and 1997, respectively. All putative QTLs for NSB accounted for 35.5%and 27.5% of total phenotypic
variation in1996 and 1997, respectively. The QTLs identified in this study will be useful intemperate japonica rice breeding
for improved spikelet yield.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
63.
Obata Y Hiura H Fukuda A Komiyama J Hatada I Kono T 《The Journal of reproduction and development》2011,57(3):327-334
Loss of imprinting (LOI) is occasionally observed in human imprinting disorders. However, the process behind the LOI is not fully understood. To gain a better understanding, we produced embryos and pups from mouse oocytes that lacked a complete methylation imprint using a method that involved transferring the nuclei of growing oocytes into the cytoplasm of enucleated fully grown oocytes following in vitro fertilization (IVF). We then analyzed the imprinting statuses. Our findings show that the incomplete methylation imprint derived from growing oocytes results in epigenetic mosaicism or a loss of methylation imprint (LOM) at maternal alleles in embryos. In some embryos, both hypo- and hypermethylated maternal Kcnq1ot1 alleles were detected, whereas either hypo- or hypermethylated maternal Kcnq1ot1 alleles were detected in others. Such tendencies were also observed at the Igf2r and Mest loci. Gene expression levels of imprinted genes were linked with their methylation statuses in some but not all embryos. Possible explanations of the inconsistency between the data from DNA methylation and gene expression include epigenetic mosaicism in embryos. Pups were successfully produced from growing oocytes at a quite low frequency. They exhibited an obese phenotype and LOI with respect to Igf2r, Snrpn and Mest. Our finding suggests the possibility that LOI/LOM at maternal alleles in human concepti could be derived from epigenetically immature/mutated oocytes. 相似文献
64.
Kurosawa T Kono Y Nagahata H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1433-1436
This study was performed to evaluate the cross section of the thymus in 46 clinically healthy Holstein calves and heifers from birth to 100 weeks of age and 8 age-matched affected animals with chronic bronchopneumonia by ultrasonography. The cross sectional area of the thymus increased in healthy calves and heifers from birth to 31 weeks of age, peaked around 35 weeks of age, and then decreased until 100 weeks after birth. A positive correlation (r = 0.74, P < 0.05) was found between the area in cross section and the weight of the whole thymus in calves and heifers with chronic bronchopneumonia. The cross- sectional areas of the thymus were significantly (P < 0.05) lowered in animals with chronic bronchopneumonia. The cross sections of thymus in clinically healthy calves at 2 weeks of age and heifers at 34 weeks and 87 weeks of age were hypoechoic, moderately hyperechoic and hyperechoic, respectively. The observation of thymus by ultrasonography seems to be an effective tool for estimating the size of the thymus objectively in Holstein calves and heifers from birth to 100 weeks of age. The results of this study prove the approach to be useful and applicable to monitor the growth of thymus in Holstein calves and heifers and their association with chronic pneumonia. 相似文献
65.
Chisholm MF Wang Y Lupini AR Eres G Puretzky AA Brinson B Melechko AV Geohegan DB Cui H Johnson MP Pennycook SJ Lowndes DH Arepalli S Kittrell C Sivaram S Kim M Lavin G Kono J Hauge R Smalley RE 《Science (New York, N.Y.)》2003,300(5623):1236; author reply 1236
66.
The lodging of crops is usually caused by wind and/or rain. However, few have studied the influence of wind force in relation to lodging. Grafius (1) blew wind of about 2.6 meters per second on barley plants and oats, and discovered that the moment of force per square centimeter on the leaves and culms was approximately one-fourth that on the heads. 相似文献
67.
Morphological aspects of rice chloroplasts isolated in glutaraldehyde and sucrose media were studied using the electron microscope. Chloroplasts in glutaraldehyde medium contained compact lamellae and densely stained stroma. On the other hand, chloroplasts in sucrose medium lacked stroma and consisted mostly of lamellae systems. 相似文献
68.
Tomoya Kono Tsuyoshi Ooyama Shih-Chu Chen & Masahiro Sakai 《Aquaculture Research》2002,33(14):1195-1197
A method for the diagnosis of nocardiosis in yellowtail (Seriola quinqueradiata), using polymerase chain reaction (PCR), was developed in this study. Primers specific for Nocardia seriolae were synthesized based on the alignment of 16S?23S rRNA internal transcribed spacer region sequences of N. seriolae. The primers did not amplify specific PCR product from other fish pathogens. However, two and three fishes could be diagnosed as infected with N. seriolae by clinical signs and bacterial isolation. PCR amplification of N. seriolae by specific primers detected six infected fishes. Thus, the primers used in this study are useful in detecting nocardiosis in fish. 相似文献
69.
70.
Takashi?Kimiya Satoshi?KubotaEmail author Michiya?Kono Mitsuaki?Hirata Haruhiko?Toyohara Katsuji?Moriora Yoshiaki?Itoh 《Fisheries Science》2005,71(3):672-678
ABSTRACT: The post-mortem changes of type I and V collagens in Japanese flounder muscle during chilled storage were examined. The muscle softened significantly within 12 h under chilled storage. Both type I and V collagens isolated by ion-exchange chromatographies showed no remarkable changes in band patterns even after 24 h of storage, suggesting that degraded collagen molecules may be removed from the collagen fraction by a conventional preparation method. In contrast, type I and V collagen molecules were detected by Western blotting with each specific antibody in a saline extract and gradually increased during chilled storage. These results suggest that both type I and V collagens may participate in the post-mortem softening of fish muscle. 相似文献