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91.
Lee CW Brown C Hilt DA Jackwood MW 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(7):835-840
Four-day-old specific-pathogen-free chickens were inoculated by eyedrop with four different strains (Gray, JMK, CV56b, and Wolgemuth) of infectious bronchitis virus (IBV). Birds were monitored clinically and euthanatized at 1, 4, 7, and 14 days postinfection and tissues were collected for virus isolation, histopathologic examination, in situ hybridization (ISH), and immunohistochemistry (IHC). Clinical disease was severe in chickens infected with Wolgemuth, but no overt disease was observed with the other strains. Virus was isolated from the kidneys of chickens infected with the Gray-, CV56b-, and Wolgemuth-strains of IBV. Histologically, interstitial nephritis was evident in chickens infected with these same 3 strains. However, viral nucleic acid and antigen were detected only with Wolgemuth-infected kidneys by ISH and IHC. These results indicate that the pathological changes in kidneys from chickens infected with Gray and CV56b may not have resulted from the cytolytic action of the virus. 相似文献
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拮抗油菜菌核病菌的链霉菌分离筛选与鉴定 总被引:1,自引:0,他引:1
从甘肃省河西走廊改良后的盐碱地分离链霉菌用于油菜菌核病菌的拮抗研究,并对拮抗菌进行鉴定。分离得到10株不同菌落形态的链霉菌,其中有2菌株对油菜菌核病有拮抗作用,编号Ⅲ22-3-3和Ⅲ22-3-12。与油菜菌核病进行对峙培养结果发现,Ⅲ22-3-3和Ⅲ22-3-12抑菌圈直径分别为1.2和0.9cm;离体叶菌丝接种试验表明,Ⅲ22-3-3和Ⅲ22-3-12对油菜菌核病菌的防治效果分别达63.5%和49.1%;Ⅲ22-3-12能在油菜菌核上定殖并寄生分解菌核,同对照相比菌核萌发率下降18.2%;通过形态培养特征、生理生化特征和16S rDNA鉴定表明,Ⅲ22-3-3为产水链霉菌(Streptomyces hydrogenans),Ⅲ22-3-12为球孢链霉菌(Streptomyces globisporus)。
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提高综合性农业科技期刊自身发展能力的探讨 总被引:3,自引:0,他引:3
简述了综合性农业期刊在竞争中发展自己的经验增强编辑的创新意识和能力,栏目策划是先导,选题策划是关键,最大限度的缩短出版时滞。紧跟学科领域的重大、重点科研项目,进行超前和后续追踪报道,瞄准生产上的热点问题进行及时报道。 相似文献
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西藏三大优良牦牛类群产乳性能及乳品质分析 总被引:10,自引:3,他引:10
1997-1998年对嘉黎、帕里、斯布三大优良类群的成年母牦牛进行了产奶性能的测定与乳品分析,结果表明帕里牦牛的产乳量最高(199.80 kg);其次是斯布牦牛(179.70 kg);嘉黎牦牛最低(147.00 kg),产乳量均低于国内外其他牦牛。乳品分析结果为帕里牦牛乳蛋白质含量为5.73 %,乳脂率为5.95 %,乳糖为3.77 %;斯布牦牛为5.27 %,7.50 %,3.49 %;嘉黎牦牛为5.10 %,6.75 %,3.56 %,并且发现嘉黎牦牛乳中含有微量的硒(0.0064 mg/kg)。 相似文献
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RONALDO C. DA COSTA JOANE PARENT HOWARD DOBSON DAVID HOLMBERG GARY PARTLOW 《Veterinary radiology & ultrasound》2006,47(6):523-531
Eighteen Doberman pinscher dogs with clinical signs of cervical spondylomyelopathy (wobbler syndrome) underwent cervical myelography and magnetic resonance (MR) imaging. Cervical myelography was performed using iohexol, followed by lateral and ventrodorsal radiographs. Traction myelography was performed using a cervical harness exerting 9 kg of linear traction. MR imaging was performed in sagittal, transverse, and dorsal planes using a 1.5 T magnet with the spine in neutral and traction positions. Three reviewers independently evaluated the myelographic and MR images to determine the most extensive lesion and whether the lesion was static or dynamic. All reviewers agreed with the location of the most extensive lesion on MR images (100%), while the agreement using myelography was 83%. The myelogram and MR imaging findings agreed in the identification of the affected site in 13-16 dogs depending on the reviewer. MR imaging provided additional information on lesion location because it allowed direct examination of the spinal cord diameter and parenchyma. Spinal cord signal changes were seen in 10 dogs. Depending on the reviewer, two to four dogs had their lesions classified as dynamic on myelography but static on MR images. Myelography markedly underscored the severity of the spinal cord compression in two dogs, and failed to identify the cause of the signs in another. The results of this study indicated that, although myelography can identify the location of the lesion in most patients, MR imaging appears to be more accurate in predicting the site, severity, and nature of the spinal cord compression. 相似文献