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11.
Zavala G Dufour-Zavala L Villegas P El-Attrache J Hilt DA Jackwood MW 《Avian diseases》2002,46(4):979-984
Unfounded field speculation has suggested that avian leukosis virus subgroup J (ALV-J) predisposes young meat-type chickens to inclusion body hepatitis caused by fowl adenovirus (FAV). To address this hypothesis, we infected 1-day-old grandparent meat-type chickens carrying maternal antibodies against FAV with a field isolate of FAV associated with inclusion body hepatitis in broilers, ALV-J, or both FAV and ALV-J. We examined the effects of FAV alone or in combination with ALV-J on the basis of clinical signs, overall mortality, growth rate, and gross and microscopic lesions. With such criteria for evaluating possible interactions, we found no significant differences in the dually infected birds in comparison with chickens that received a monovalent challenge with either FAV or ALV-J. 相似文献
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介绍了原木蒸煮废水的处理方法,提出氧化塘法是小型木材加工厂处理该废水的最佳选择,并用实例简单介绍了氧化塘的设计选型问题. 相似文献
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Abstract. Four experimental diets were formulated to resemble practical rainbow trout, Salmo gairdneri Richardson, grower feeds based on medium and moderate lipid inclusions of 10 and 14% (standard energy levels) to relatively 'high energy' diets containing 18 and 22% lipid respectively. Each of these diets was isonitrogenous with a defined protein content of 42%. During a 10-week growth trial period, juvenile rainbow trout of initial mean weight of 10g were fed each ration to satiation. At the end of the study, there was a significant gain (P> 0.05) in the mean live weights of fish receiving the higher energy diets associated with improved feed conversion ratio (FCR) and net protein utilization (NPU). Although there was a declining trend in voluntary food intake relative to body weight with increasing dietary lipid content, this was insufficient to prevent greater deposition of body fat.
The results are discussed in relation to practical trout feeds in order to achieve a desired growth performance and acceptable body composition with intensive feeding regimes. 相似文献
The results are discussed in relation to practical trout feeds in order to achieve a desired growth performance and acceptable body composition with intensive feeding regimes. 相似文献
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兰州大尾羊心脏型脂肪酸结合蛋白(H-FABP) 基因克隆及其同源性比较 总被引:1,自引:1,他引:1
【目的】克隆兰州大尾羊心脏型肪酸结合蛋白(H-FABP)基因全长cDNA序列,为研究绵羊H-FABP生物学作用和生产应用提供理论依据。【方法】根据已知哺乳动物H-FABP基因 cDNA 序列,设计5''和3''特异引物,运用cDNA 末端快速扩增(RACE)技术获得兰州大尾羊H-FABP基因全长 cDNA 序列。【结果】 扩增获得兰州大尾羊5''端425 bp、3''端231 bp片段和 177 bp中间片段,拼接获得748 bp兰州大尾羊H-FABP基因全长cDNA 序列(GenBank登录号:JQ780322)。 兰州大尾羊H-FABP基因ORF长 402 bp,编码 133 个氨基酸。核苷酸序列分析显示兰州大尾羊H-FABP基因序列与大多数哺乳动物相似,但其第66位发生的碱基转换(T←→G)引起所编码的第22位天门冬氨酸(N)不同于其它所有物种的赖氨酸(K)。构建的基因进化树分析结果显示兰州大尾羊与山羊亲缘关系最近。预测兰州大尾羊H-FABP蛋白质的空间结构与山羊和牛H-FABP类似,由2个α螺旋和10个反向平行的β折叠组成,10 个折叠片围成一个桶状结构,疏水性残基位于桶内,用于结合脂肪酸。【结论】克隆了兰州大尾羊H-FABP基因,为进一步研究该基因的功能奠定了基础。 相似文献
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Spike glycoprotein cleavage recognition site analysis of infectious bronchitis virus. 总被引:13,自引:0,他引:13
The spike glycoprotein of infectious bronchitis virus (IBV), a coronavirus, is translated as a precursor protein (So), then cleaved into two subunits (S1 and S2) by host cell serine proteases. In this study, we compared the cleavage recognition site of 55 IBV isolates to determine if the cleavage recognition site sequence, which consists of five basic amino acid residues, correlates with host cell range, serotype, geographic origin, and pathogenicity as it does in orthomyxoviruses and paramyxoviruses. The most common cleavage recognition site observed (33 of 55 viruses) was Arg-Arg-Ser-Arg-Arg, representing at least 11 different serotypes. Thus, cleavage recognition site does not appear to correlate with serotype. We also determined that cleavage recognition site sequence does not correlate with pathogenicity because attenuated and pathogenic isolates (different passages of the same virus) contain identical cleavage recognition site sequences. In addition, nephropathogenic strains had the same cleavage recognition site sequence as many nonnephropathogenic isolates. Cleavage recognition site sequence does correlate with viruses in different geographic regions, which may be an important characteristic to examine in epidemiologic studies. An IBV monoclonal antibody neutralization-resistant mutant (NR 18) had an unusual substitution of Ile for Arg at the fourth position, giving the sequence Arg-Arg-Ser-Ile-Arg, which likely prevents cleavage and, thus, destroys the conformationally dependent monoclonal antibody binding epitope. Six residues on the amino-terminal side of the cleavage recognition site are conserved in 31% of the isolates and consist of only one or two basic amino acids. Thus, the number of basic residues around the cleavage recognition site does not appear to correlate with increased cleavability, host cell range, and increased virulence as it does with envelope glycoproteins in orthomyxoviruses and paramyxoviruses. 相似文献
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为了检测牛GDF-9基因mRNA在怀孕期牛生殖系统的表达情况,为GDF-9基因在雌性动物繁殖上的研究提供一定的理论依据。本试验根据GenBank中报道的牛GDF-9基因序列设计引物,从牛卵巢、输卵管、子宫、肾脏中提取RNA,采用RT-PCR技术进行cDNA扩增,扩增产物与载体pGM-T连接,转化大肠杆菌DH5α,筛选阳性克隆,测序。结果表明,已成功克隆的阳性重组子经测序鉴定其片段大小与预期大小完全一致,为264 bp。序列同源性分析比较表明,该cDNA及其推导的氨基酸序列与绵羊GDF-9cDNA和氨基酸序列同源性分别为97%和94%。以-βactin为内参照物,采用RT-PCR技术进行半定量分析,通过检测比较牛GDF-9基因在卵巢、输卵管、子宫、肾脏组织中mRNA的表达情况。结果表明,牛GDF-9基因在卵巢中表达量最高,在输卵管、子宫、肾脏中均有表达,但表达量不高。 相似文献
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上海乡土树种及其在城市绿化建设中的应用 总被引:34,自引:1,他引:34
随着生态城市被列为上海市的建设发展目标,城市绿化建设被赋予了新的目标和内涵,生态林将逐渐成为城市绿化的主体,作为生态林建设基础的乡土树种也将得到更为广泛的应用.通过实地调查和资料调查,统计分析了上海市的乡土树种,共42科89属128种(含变种),可以分为常绿阔叶乔木、常绿阔叶灌木、半常绿阔叶灌木、落叶阔叶乔木、落叶阔叶小乔木和落叶阔叶灌木等6个类型.分析了乡土树种在上海绿化建设中的意义和应用现状,并对其应用进行了展望.表1参21 相似文献