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81.
There is now clear evidence for a prolonged increase in atmospheric CO2 concentrations and enrichment of the biosphere with N. Understanding the fate of C in the plant-soil system under different CO2 and N regimes is therefore of considerable importance in predicting the environmental effects of climate change and in predicting the sustainability of ecosystems. Swards of Lolium perenne were grown from seed in a Eutric Cambisol at either ambient (ca. 350 μmol mol−1) or elevated (700 μmol mol−1) atmospheric pCO2 and subjected to two inorganic N fertilizer regimes (no added N and 70 kg N ha−1 month−1). After germination, soil solution concentrations of dissolved organic C (DOC), dissolved inorganic N (DIN), dissolved organic N (DON), phenolics and H+ were measured at five depths down the soil profile over 3 months. The exploration of soil layers down the soil profile by roots caused transient increases in soil solution DOC, DON and phenolic concentrations, which then subsequently returned to lower quasi-stable concentrations. In general, the addition of N tended to increase DOC and DON concentrations while exposure to elevated pCO2 had the opposite effect. These treatment effects, however, gradually diminished over the duration of the experiment from the top of the soil profile downwards. The ambient pCO2 plus added N regime was the only treatment to maintain a notable difference in soil solution solute concentration, relative to other treatments. This effect on soil solution chemistry appeared to be largely indirect resulting from increased plant growth and a decrease in soil moisture content. Our results show that although plant growth responses to elevated pCO2 are critically dependent upon N availability, the organic chemistry of the soil solution is relatively insensitive to changes in plant growth once the plants have become established. 相似文献
82.
Faeces from 20 calves aged 13 +/- 5 days were examined for Toxocara vitulorum eggs fortnightly for a period of 20 weeks. The age of calves when eggs were first detected varied from 24 to 110 days (mean 41 +/- 20 days). The mean egg count varied from 257 to 19,821 g-1 of faeces with a highest count of 68,400. The patency period of T. vitulorum lasted from 14 to 112 days (mean 65 +/- 30 days). None of the calves showed clinical signs of illness. 相似文献
83.
Yield of red cherry tomatoes and soil salt distribution as affected by different amounts and salinities of irrigation water was studied in a field plot experiment during the summer of 1988 at Utah State University. Four different amounts of fresh water and two amounts of saline water were imposed with line source trickle irrigation on a sandy loam soil in lysimeters. Tomato yield and seasonal evapotranspiration increased in a linear fashion with increasing irrigation water. Tomato yields with fresh water (EC = 0.33 dS/m) were significantly higher than with saline (EC = 4.0 dS/m) water. There were no significant differences in seasonal evapotranspiration between the two salinity levels with two levels of deficit irrigation. Soil water depletion and availability decreased as the soil water salinity increased. Soil water salinities increased with both vertical and horizontal distance from trickle lines and reached a maximum at the bottom of the wetted area and between trickle lines. 相似文献
84.
Subcellular distribution of copper in the liver of fetal deer in the last month of gestation 总被引:2,自引:0,他引:2
M J Leighton T J Peters R Hill G D Smith W Holt D M Jones 《Research in veterinary science》1990,49(3):298-305
Fetal deer, in the last month of gestation, accumulate high concentrations of copper in the liver. Livers from fetal deer in late gestation were homogenised and fractionated by continuous sucrose density gradient centrifugation. The distribution of copper closely followed that of DNA; approximately two thirds of the metal was localised to the nuclear fractions with the remaining third in the cytosolic fractions. The fractionation procedure was repeated with digitonin, a lysosomal perturbant: lysosomes were disrupted and the marker enzyme, N-acetyl-beta-glucosaminidase, shifted from the particulate fractions to the cytosolic fractions; the distribution of copper was unaffected. Differential centrifugation of homogenate confirmed that approximately two thirds of the copper was associated with the nuclear fraction. Further confirmation of a nuclear localisation of copper was provided by X-ray microanalysis of purified nuclei. 相似文献
85.
Muscle contraction and free energy transduction in biological systems 总被引:52,自引:0,他引:52
Muscle contraction occurs when the actin and myosin filaments in muscle are driven past each other by a cyclic interaction of adenosine triphosphate (ATP) and actin with cross-bridges that extend from myosin. Current biochemical studies suggest that, during each adenosine triphosphatase cycle, the myosin cross-bridge alternates between two main conformations, which differ markedly in their strength of binding to actin and in their overall structure. Binding of ATP to the cross-bridge induces the weak-binding conformation, whereas inorganic phosphate release returns the cross-bridge to the strong-binding conformation. This cross-bridge cycle is similar to the kinetic cycle that drives active transport and illustrates the general principles of free energy transduction by adenosine triphosphatase systems. 相似文献
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