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61.
Matsumoto H Ikoma Y Sugiura M Yano M Hasegawa Y 《Journal of agricultural and food chemistry》2004,52(21):6653-6659
Hesperidin is a biologically effective flavonoid. Several studies have reported that dietary hesperidin was converted to conjugated metabolites, such as hesperetin-glucuronides and sulfoglucuronides, during absorption and metabolism. However, the chemical structures of the conjugated metabolites, especially the sites of glucuronidation and sulfoglucuronidation in plasma, were unconfirmed. Therefore, the concentrations of the metabolites conjugated at various sites in plasma could not be individually quantified. In the present study, we identified the chemical structures and concentrations of the major conjugated metabolites in rat plasma after oral administration of hesperidin. Two hesperetin-glucuronides were prepared and identified as hesperetin-7-O-beta-D-glucuronide and hesperetin-3'-O-beta-D-glucuronide. Using these authentic compounds, the concentrations of hesperetin-7-O-beta-D-glucuronide and hesperetin-3'-O-beta-D-glucuronide in rat plasma were individually determined by liquid chromatography-mass spectrometry. In rat plasma, hesperetin-glucuronides were primarily comprised of hesperetin-7-O-beta-D-glucuronide and hesperetin-3'-O-beta-D-glucuronide. The concentration of hesperetin-7-O-beta-D-glucuronide was slightly higher than that of hesperetin-3'-O-beta-D-glucuronide. Furthermore, not only hesperetin conjugates but also homoeriodictyol conjugates were observed in rat plasma. The present study is the first report elucidating the chemical structures and changes in individual concentrations in rat plasma of glucuronides derived from orally administered hesperidin. 相似文献
62.
63.
Fujiko MinamiMakoto Nagai Mika ItoTatsuhiko Matsuda Hikaru TakaiYoshiko Jinkawa Takeshi ShimanoMichiko Hayashi Yoshihisa SekiYoshihiro Sakoda Katsuaki SugiuraHiroomi Akashi 《Comparative immunology, microbiology and infectious diseases》2011,34(1):35-39
Bovine viral diarrhea virus (BVDV) field isolates show genetic and antigenic diversity. At least 14 subgenotypes of BVDV-1 and 4 of BVDV-2 have been identified in Artiodactyla worldwide. Of these, 6 subgenotypes of BVDV-1 and 1 of BVDV-2 have been isolated in Japan. Previously, we reported that each subgenotype virus expresses different antigenic characteristics. Here we investigated the reactivity of neutralizing antibodies against representative strains of Japanese BVDV subgenotypes using sera from 266 beef cattle to estimate the prevalence of this epidemic virus among cattle in Japan. Antibody titers at concentrations at least 4-fold higher than antibodies against other subgenotype viruses were considered subgenotype specific. Subgenotype-specific antibodies were detected from 117 (80.7%) of 145 sera samples (69.7% against BVDV-1a, 1.4% against BVDV-1b, 8.3% against BVDV-1c, and 1.4% against BVDV-2a). The results suggest that neutralization tests are useful in estimating currently epidemic subgenotypes of BVDV in the field. 相似文献
64.
Lignin prepared by a modified Klason method (KSL) was modified with ozone followed by alkaline treatments. The original and
modified KSLs were subjected to a plant growth test with radish (Raphanus sativus L. var. radicula Pers.) in the presence of AlCl3 (37.5 μM) to assess their ability to reduce aluminum toxicity. The formation of an aluminum complex with these KSLs was also
examined by potentiometry. Neither the original nor alkaline-treated KSLs had an effect on aluminum toxicity or the formation
of a complex, except for the alkaline-treated KSL at a maximal dose of 250 mg/l, which reduced aluminum toxicity. However,
all the KSLs modified with ozone and alkali were effective at forming a complex and reducing aluminum toxicity. The dose of
saponified ozone-treated KSLs required to reduce aluminum toxicity became lower and their water solubility increased as the
ozone treatment was prolonged. These findings clearly show that water solubility and the ability to form a complex with and
reduce the toxicity of aluminum were improved by ozone treatment of KSL. 相似文献
65.
66.
Hikaru Komatsu Yuka Onozawa Tomonori Kume Kenji Tsuruta Tomo’omi Kumagai Yoshinori Shinohara Kyoichi Otsuki 《Forest Ecology and Management》2010
In western Japan, Moso bamboo (Phyllostachys pubescens) forests have been expanding by replacing surrounding vegetation such as coniferous plantation forests and natural broadleaved forests. It has been speculated that the replacement of surrounding vegetation by bamboo forests could alter the vegetation water cycle and available water resources. We quantified stand-scale transpiration (E) in a bamboo forest on the basis of sap-flux measurements and compared the E value with values for coniferous forests. The annual E was estimated to be 567 mm. Seasonal trends in E primarily corresponded to seasonal trends in the vapor pressure deficit. Annual E for the bamboo forest was higher than that for the coniferous forests by 12% of annual precipitation (P). This difference in annual E is comparable with the difference in annual interception evaporation (I) relative to P between bamboo and coniferous forests; previous studies reported lower I for bamboo forests by ∼10% of P. Thus, the sum of E and I was comparable for bamboo and coniferous forests. As this study is the first measuring E of bamboo forests, further studies are required to examine the generality of our results. 相似文献
67.
Biogeography of small odontocetes in relation to wide‐scale oceanographic structure in the North Pacific Ocean
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Information regarding the distributional patterns of top predators is undoubtedly important for the monitoring and management of marine ecosystems. Nevertheless, previous knowledge on the distributional patterns of cetacean species in relation to physical oceanography is usually limited to a small fraction of each species' range because of their vast distribution and infrequency of encountering many species. We carried out comprehensive analyzes on the distributions of small odontocetes covering almost the entire North Pacific Ocean. Using data from over 300 000 nautical miles (≈ 555 600 km) of dedicated sighting surveys and 7000 school sightings of 14 small odontocete species, which have accumulated for approximately a quarter of a century, we describe interspecific habitat differences in small odontocetes with respect to physical oceanography. Multivariate analyzes were used to discriminate the 14 species into four ecological groups: (i) Subtropical Domain species; (ii) Subarctic Boundary species; (iii) Transitional Domain species; and (iv) Subarctic Domain species. By comparing environmental variables in their habitats with variables indicative of water mass and frontal structures, the four species groups were further divided into several subgroups. Groups at higher latitudes comprised fewer species than those at lower latitudes, while species diversity was maximal at lower and intermediate latitudes. Our findings indicate that large‐scale oceanographic structures are important determinants for the present patterns in biogeography and species diversity of small odontocetes. 相似文献
68.
Sheikh M. Masum Mohammad Amzad Hossain Hikaru Akamine Jun‐Ichi Sakagami Prasanta C. Bhowmik 《Weed Biology and Management》2016,16(3):119-131
A series of experiments was conducted in the laboratory and greenhouse of the Subtropical Field Science Center, University of the Ryukyus, Japan, from April to October 2015 to assess the allelopathic potential of 50 indigenous Bangladeshi rice varieties by using the donor–receiver bioassay, equal compartment agar method (ECAM), plant residue extract method and pot culture method. Lettuce (Lactuca sativa L.), cress (Lepidium sativum L.), radish (Raphanus sativus L.), barnyard grass (Echinochloa crus‐galli L. Beauv.) and jungle rice (Echinochloa colona L.) were used as the test plants. The highest inhibition effect was given by Boterswar, while the stimulating effect was given by Kartikbalam and Panbira in the donor–receiver bioassay and ECAM tests. Boterswar, Goria, Biron and Kartiksail were selected as the highest allelopathic‐potential varieties by the donor–receiver bioassay and ECAM. In the methanol extract test, Boterswar gave the strongest inhibitory effect on both barnyard grass and jungle rice, while Kartiksail gave the highest inhibitory effect on the jungle rice shoot. The growth parameters and total dry matter of barnyard grass in the greenhouse pot experiment were significantly reduced as a result of the application of aqueous extracts of the selected rice varieties, which was similar to the results of the laboratory experiments. The varieties of Boterswar, Goria, Biron and Kartiksail were selected as the most allelopathic among the 50 indigenous Bangladeshi rice varieties. These rice varieties could be used for the isolation and identification of allelochemicals and to further develop new varieties that are tolerant to weeds. 相似文献
69.
Hikaru Watanabe Makoto Okazaki Tsutomu Tamura Kenji Konishi Denzo Inagake Takeharu Bando Hiroshi Kiwada Tomio Miyashita 《Fisheries Science》2012,78(3):557-567
This study represents the first quantitative analysis of the characteristics of the distribution areas and stomach contents
of common minke whale Balaenoptera acutorostrata, sei whale B. borealis, and Bryde’s whale B. edeni in relation to oceanographic and prey environments in mid summer in the western North Pacific. Common minke whales were distributed
within subarctic regions and the northernmost region of the transitional domain, coinciding with the main habitat of their
preferred prey, Pacific saury Cololabis saira. Sei whales were mainly found in the northernmost part of the transition zone and showed prey preference for Japanese anchovy
Engraulis japonica, which was significantly more abundant in the main distribution area of the whale than in its adjacent areas. “Hot spots”
of Bryde’s whales were found in several regions of the transition zone between the subarctic boundary and the Kuroshio front.
This whale species preferred Japanese anchovy as prey, for which the distribution density was significantly higher in the
main distribution area of the whale than in the adjacent areas. These results indicate that the summer distributions of Pacific
saury and Japanese anchovy greatly influence the distributions of these whale species, suggesting that the whales’ habitat
selection is closely related to their prey selection. 相似文献
70.
Classification of lactic acid bacteria isolated from chigee and mare milk collected in Inner Mongolia 总被引:1,自引:0,他引:1
One hundred and seventeen isolates from chigee and 191 isolates from mare milk collected in Inner Mongolia were classified into six and 10 groups, respectively, based on the protein profiles obtained by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). A dendrogram on the basis of the molecular sizes of the protein bands was drawn and the similarity among the groups ranged from 96% to 44%. Representative isolates from all groups were identified by 16S rDNA sequencing and a phylogenetic tree was drawn. Furthermore, the results of carbohydrate fermentation tests of all groups were compared with those obtained by 16S rDNA sequencing and SDS‐PAGE analyzes. The isolates from chigee consisted of mainly Lactobacillus sp., Lactobacillus (Lb.) plantarum, Lb. pentosus and Lactococcus (Lc.) lactis ssp. cremoris were isolated at the rates of 48, 33 and 19%, respectively. The isolates from mare milk consisted of coccus, namely, Leuconostoc (Leuc.) mesenteroides, Leuc. pseudomesenteroides, Lc. garvieae, Lc. lactis ssp. lactis, Streptococcus (Sc.) parauberis and Enterococcus (Ec.) faecium. The isolation rates were 45, 19, 7, 8, 16 and 6%, respectively. The results demonstrated that the species isolated from chigee were different from those in mare milk. 相似文献