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101.
Yutaka Taguchi Yudai Inabu Koki Hayasaki Noriyuki Maeda Yoshiro Kanmera Seiji Yamasaki Noboru Ota Kenji Mukawa Arisa Tsuboi Hirokuni Miyamoto Tetsuji Etoh Yuji Shiotsuka Ryoichi Fujino Christopher D. McMahon Hideyuki Takahashi 《Animal Science Journal》2021,92(1):e13505
We evaluated the effects of feeding high volumes of milk replacer on growth and reproductive performances in Japanese black heifers. Fifty-one heifers were fed milk replacer at 9 L/day for 60 days (9 L × 60 days; n = 18) or 41 days (9 L × 41 days; n = 15), or at 7 L/day for 40 days (7 L × 40 days; n = 18). Artificial insemination (AI) was performed on heifers with ≥270 kg body weight and ≥116 cm body height at 300 days of age. The age at the first AI was 0.35 month later for 7 L × 40 days than the other groups (p < .01). However, age at calving did not differ among treatments (22.1 months). The interval from the first AI to pregnancy tended to be ~2 months longer for the 9 L × 60 days than the other groups (p = .07). Our results showed that feeding high volumes of milk replacer may reduce the age at calving via an improved rate of growth. In addition, we propose that feeding a maximum of 7 L milk replacer for 40 days may be the most appropriate rearing regime because the success of pregnancy per AI may be reduced in calves fed a maximum of 9 L for 41 and 60 days. 相似文献
102.
The recent discovery of a hyper-metal-poor (HMP) star, with a metallicity Fe/H smaller than 1/100,000 of the solar ratio, together with one earlier HMP star, has raised a challenging question whether these HMP stars are the actual first-generation, low-mass stars of the universe. We argue that these HMP stars are second-generation stars formed from gases that were chemically enriched by the first-generation supernovae. The key to this solution is the very unusual abundance patterns of these HMP stars and the similarities and differences between them. We can reproduce these abundance features with core-collapse "faint" supernova models that include extensive matter mixing and fallback during explosions. 相似文献
103.
Al‐Nur Md. Iftekhar Rahman Seiya Yamashita Md. Rashedul Islam Taisuke Fujihara Hayato Yamaguchi Manabu Kawahara Masashi Takahashi Hideyuki Takahashi Takafumi Gotoh Nobuhiko Yamauchi 《Animal Science Journal》2020,91(1)
This study investigated the effect of type‐I interferon (IFN) on the expression of matrix metalloproteinases (MMPs) of the bovine endometrial stromal cells (BES) and epithelial cells (BEE). The cells were separated and purified from the caruncles and cultured in DMEM/F‐12 containing 10% fetal bovine serum. Spheroids were generated by using ascorbate. Zymograms of the supernatant showed that BEE predominantly expressed MMP‐9, whereas MMP‐2 was expressed in BES and homo‐spheroids. While MMPs expression was not detected in hetero‐spheroids. Real‐time quantitative PCR revealed that type‐I IFN and P4 suppressed the gene expression of MMP‐2 and MMP‐9 in hetero‐spheroids, respectively. On the other hand, gelatin zymography analysis of the supernatant showed that type‐I IFN strongly promote the clearance of MMPs. While zymograms of the MMPs stocked in the hetero‐spheroids were significantly reduced by type‐I IFN. Phenylmethanesulfonyl fluoride and leupeptin (both are serine proteinase inhibitors) significantly repressed the clearance of MMP‐2 and MMP‐9 induced by type‐I IFN. Moreover, collagen fibers in hetero‐spheroids significantly decreased after the treatment with type‐I IFN. In conclusion, it was suggested that type‐I IFN participate in the tissue remodeling by regulation the clearance of MMPs. 相似文献
104.
Tanaka S Mannen K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):351-354
Butorphanol tartrate (BT) was injected into mice before injection with acetylcholine in a murine model infected latently with pseudorabies virus. The analgesic effect and its influence on virus reactivation were observed. Mice preinjected with BT showed suppression of screaming, moving and excitation and the same level of movement after excitation as mice injected with PBS. In the group injected with BT i.p., one mouse died and another developed diarrhea with increased virus excretion. These results showed that BT has analgesic effects by both injection routes, s.c. and i.p.; however, BT induced death as a side effect, especially with i.p. injection. The injection route for BT should therefore be investigated further. 相似文献
105.
Makoto KANEDA Bang Zhong LIN Shinji SASAZAKI Kenji OYAMA Hideyuki MANNEN 《Animal Science Journal》2011,82(6):717-721
Allele frequencies of 10 representative polymorphisms for beef and milk traits were investigated for a total of 240 animals from Bos taurus and Bos indicus breeds, including two Japanese groups (Japanese Black and Japanese Brown), two East Asian groups (Korean and Mongolian), three European groups (Holstein, Angus and Hereford) and a Bos indicus group in South Asia (Myanmar, Laos and Cambodia). The Japanese Black revealed unique genetic construction in GH, FASN and SREBP‐1 and the other Asian populations show intermediate frequencies between European and Japanese populations. The Bos indicus group showed low favorable allele frequencies in most of the genes. The study showed the variability and distribution of 10 genes affecting economic traits among world representative cattle breeds. The genetic information would contribute to elucidating the genetic background for worldwide cattle breeds and the possibility of improvement using the markers. 相似文献
106.
Shinji SASAZAKI Daisuke HOSOKAWA Rie ISHIHARA Hiroki AIHARA Kenji OYAMA Hideyuki MANNEN 《Animal Science Journal》2011,82(1):67-72
In the meat industry, correct labeling of beef origins or breed is required to assure quality and safety. This paper describes the development of discrimination markers between Japanese domestic and imported beef from the United States (US) and Australia (AUS) based on a bovine 50K single nucleotide polymorphism (SNP) array using a total of 110 samples: Japanese Black (n = 50), Japanese Holstein (n = 50) and US cattle (n = 10). Genotyping information revealed 1081 SNPs as candidate markers that were polymorphic only in US cattle. The genotyping results by PCR – restriction length polymorphism in Japanese Black (n = 300) and Holstein cattle (n = 146) revealed that 11 SNPs had alleles specific to US cattle. Their allelic frequencies in US cattle (n = 108) ranged from 0.097 to 0.250 with an average of 0.178 and the combined identification probability of US cattle was 0.987. In addition, we also verified the applicability of these US‐specific markers to AUS cattle. Their allelic frequencies in AUS cattle (n = 280) ranged from 0.063 to 0.224 with an average of 0.137 and the combined identification probability of AUS cattle was 0.963. In conclusion, a set of these markers could be useful for discriminating between Japanese domestic and imported beef and would contribute to identify origins and prevent falsified labeling of beef. 相似文献
107.
Shiro Suzuki Kunihiro Suda Nozomu Sakurai Yoshiyuki Ogata Takefumi Hattori Hideyuki Suzuki Daisuke Shibata Toshiaki Umezawa 《Journal of Wood Science》2011,57(1):40-46
Acacia mangium is a fast-growing tree widely planted in tropical countries because of its rapid growth, high wood density, high fiber quality,
and good adaptability. Despite its importance as a fiber source in the pulp and paper industry, a large-scale analysis of
expressed sequence tags (ESTs) has not been performed in A. mangium. In this study, we sequenced 10 752 clones of a normalized complementary DNA (cDNA) library prepared from A. mangium developing secondary xylem and shoot, and obtained a total of 8963 ESTs. The ESTs were assembled into 6220 unigenes comprising
1614 contigs and 4606 singletons. The unigene set was then subjected to various bioinformatic analyses. BlastN searches of
the unigene set against the Gene Index Databases of soybean, Medicago truncatula, Lotus japonicus, grape, poplar, spruce, and pine demonstrated that the largest number of unigenes shared homologies with the soybean Gene
Indices. BlastX searches against the TAIR9 peptide database enabled us to annotate the unigenes. Based on the annotation,
we discussed whether the unigenes involved in the cell cycle, cell growth, shoot apical meristem development, and cell wall
biosynthesis were present. This new genomic resource will accelerate the functional genomics of wood formation and molecular
breeding to improve the wood properties of A. mangium. 相似文献
108.
Shinji Sasazaki Kento Akiyama Takahiro Narukami Hirokazu Matsumoto Kenji Oyama Hideyuki Mannen 《Animal Science Journal》2014,85(5):499-505
Fatty acid composition of beef adipose tissue is one of its important traits because a high proportion of monounsaturated fatty acid is related to favorable beef flavor and tenderness. In this study, we searched polymorphisms in full length coding DNA sequence of urotensin 2 recepter and investigated the effects on fatty acid composition (C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, monounsaturated fatty acid, saturated fatty acid). Eight single nucleotide polymorphisms (SNP) were identified by sequence comparison among eight animals, including five Japanese Black and three Holstein cattle. One of these SNP (c.866C>T) was predicted to cause amino acid substitutions (P289L) and the other seven synonymous SNP, including c.267C>T, were presumed to be in linkage disequilibrium. Therefore we selected two SNP (c.267C>T and c.866C>T) for further analysis. We investigated associations between these genotypes and fatty acid composition in three Japanese Black populations (n = 560, 245 and 287) and a Holstein population (n = 202). Tukey‐Kramer's honestly significant difference test revealed that CC genotype in c.267C>T indicated lower C14:0 and higher C18:1 than the other genotypes in Japanese Black cattle and CC genotype in c.866C>T showed lower C16:1 than CT genotype in Holstein cattle (P < 0.05). These results suggested that these genotypes would contribute to production of high‐grade meat as selection markers in beef cattle. 相似文献
109.
目的 探讨醒斑银弄蝶Carterocephalus alcina Evans, 1939与其近似种的关系。方法 广泛采集醒斑银弄蝶及其近似种样本,基于形态特征、COI条形码及地理分布进行关系分析。结果 黄斑银弄蝶C. alcinoides Lee, 1962应是醒斑银弄蝶C. alcina的个体变异,故为其新异名;产自北京的2个银弄蝶标本与其近似种间的最小平均遗传距离为3.9%,在系统树上独立成支,前翅中室基部和后翅前缘中部有斑且后翅M1-2室斑延伸至外缘,为其独有;醒斑银弄蝶、北京支系、银弄蝶C. palaemon和黄翅银弄蝶C. silvicola呈高度支持的单系支。结论 黄斑银弄蝶是醒斑银弄蝶的新异名;产自北京的近似于醒斑银弄蝶的种群是一个新种,即长斑银弄蝶Carterocephalus longimaculatus Hou, Fan & Li sp. nov.。 相似文献
110.
Hidetoshi Ishibashi Hideyuki H. Motohashi Mami Kumon Kazuhiro Yamamoto Hironori Okada Takashi Okada Kazuhiko Seki 《Animal Science Journal》2013,84(11):740-743
The use of the common marmoset monkey in biomedical research has increased recently, and further attention has been devoted to this model after the successful production of transgenic marmosets. To extend genetic engineering approaches to widespread biomedical research fields, efficient prolonged in vitro culturing of embryo development is necessary. We aimed to evaluate the effects of the size of the zona pellucida opening on promoting the hatching process in the marmoset embryo. Piezo‐microdrilling of a 6‐μm opening in eight embryos resulted in four partially hatched embryos and one hatched embryo after 5 days of culture. Piezo‐microdrilling a 20‐μm opening in 11 embryos resulted in nine partial hatchings and no hatched embryos. Piezo‐scraping an 80‐μm opening in six embryos resulted in no partially hatched embryos and five hatched embryos. These results suggest that an 80‐μm opening, rather than 6‐μm or 20‐μm openings, is suitable to complete the hatching process in the marmoset embryo. 相似文献