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91.
Decha Pangjai Soichi Maruyama Sumalee Boonmar Hidenori Kabeya Shingo Sato Burin Nimsuphan Wimol Petkanchanapong Wattanapong Wootta Piyada Wangroongsarb Maskiet Boonyareth Poom Preedakoon Watcharee Saisongkorh Pathom Sawanpanyalert 《Comparative immunology, microbiology and infectious diseases》2014
We investigated the prevalence of Bartonella species in 10 rodent and one shrew species in Thailand. From February 2008 to May 2010, a total of 375 small animals were captured in 9 provinces in Thailand. Bartonella strains were isolated from 57 rodents (54 from Rattus species and 3 from Bandicota indica) and one shrew (Suncus murinus) in 7 of the 9 provinces, and identified to the species level. Sequence analysis of the citrate synthase and RNA polymerase β subunit genes identified the 58 isolates from each Bartonella-positive animal as B. tribocorum in 27 (46.6%) animals, B. rattimassiliensis in 17 (29.3%) animals, B. elizabethae in 10 (17.2%) animals and B. queenslandensis in 4 (6.9%) animals. R. norvegicus, R. rattus, and Suncus murinus carried B. elizabethae, which causes endocarditis in humans. The prevalence of Bartonella bacteremic animals by province was 42.9% of the animals collected in Phang Nga, 26.8% in Chiang Rai, 20.4% in Sa Kaeo, 16.7% in Nakhon Si Thammarat, 12.0% in Surat Thani, 9.1% in Mae Hong Son and Loei Provinces. 相似文献
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Eiko Touno Makoto Kaneko Sunao Uozumi Hidenori Kawamoto Shin Deguchi 《Animal Science Journal》2014,85(1):46-52
Twelve sheep were used in a 3 × 3 Latin square design to evaluate the effect of substituting wheat bran with forage soybean silage in the diet on apparent digestibility and nitrogen balance. Forage soybean (Glycine max (L.) Merrill) was cultivated in a no‐till, no‐herbicide cropping system with Italian ryegrass (Lolium multiflorum Lam.) as a living mulch. Forage soybean and Italian ryegrass were wilted and ensiled in round bales without additives, respectively. The experimental diets were based on corn silage supplemented with protein sources (tow silages or wheat bran). The crude protein and the acid detergent insoluble protein contents of forage soybean silage were the highest among the protein sources. The apparent digestibility of crude protein and the nitrogen balance did not significantly differ among the diets. In addition, the phytoestrogen content of forage soybean silage was below the level at which animal reproductive performance would be negatively affected. These results suggest that forage soybean silage has comparable feeding value to wheat bran, and can be given at an inclusion level of 17% (dry matter basis) as an alternative protein source to wheat bran without adverse effects on digestion or nitrogen balance in sheep fed a corn silage‐based diet. 相似文献
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Jinghui LIU Mohamed A. MAREY Rasoul KOWSAR Nina HAMBRUCH Takashi SHIMIZU Shingo HANEDA Motozumi MATSUI Motoki SASAKI Hiroyuki HAYAKAWA Christiane PFARRER Akio MIYAMOTO 《The Journal of reproduction and development》2014,60(5):342-348
We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological
conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid
glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA
is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this
study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity
of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP
gene was expressed in cultured bovine oviduct epithelial cells (BOECs) and AGP protein was detected in oviduct fluid.
Preexposure of PMNs to AGP at physiological levels impaired PMN phagocytosis for sperm and superoxide generation. The
desialylation of AGP eliminated these suppressive effects of AGP on PMN. Scanning electron microscopy revealed that AGP
drastically reduced the formation of DNA-based neutrophil extracellular traps (NETs) for sperm entanglement. Additionally,
AGP dose-dependently stimulated BOECs to produce prostaglandin E2 (PGE2) which has been shown to
partially contribute to the regulation of sperm phagocytosis in the bovine oviduct. AGP and PGE2 at concentrations
detected in the oviducts additively suppressed sperm phagocytosis by PMNs. These results provide evidence that locally
produced AGP may be involved in protecting sperm from phagocytosis by PMNs in the bovine oviduct. 相似文献
97.
Chahan B Jian Z Xuan X Sato Y Kabeya H Tuchiya K Itamoto K Okuda M Mikami T Maruyama S Inokuma H 《Veterinary parasitology》2005,134(3-4):273-278
Serological methods were utilized to detect Anaplasma and Ehrlichia infection in domestic animals in Xinjiang Uygur Autonomous Region, China. By using an indirect immunofluorescence assay (IFA), antibodies that reacted with Anaplasmaphagocytophilum and Ehrlichiachaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were recorded. These results indicate that ruminants kept in these areas may be infected with some species of Anaplasma and Ehrlichia. 相似文献
98.
Verdida RA Hara OA Xuan X Fukumoto S Igarashi I Zhang S Dong J Inokuma H Kabeya H Sato Y Moritomo T Maruyama S Claveria F Nagasawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1517-1521
The surface antigen P50 of Babesia gibsoni is an important candidate for the development of a diagnostic reagent for canine piroplasmosis. In order to establish an effective diagnostic method for practical use, the gene encoding truncated P50 (P50t) lacking a signal peptide and C-terminal hydrophobic regions were cloned and expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST). More than 90% portion of the GST-P50t was expressed as a soluble form, in contrast with GST-P50f (full-length), which was completely expressed as an insoluble form. This result indicates that removal of the hydrophobic signal peptide and C-terminus had dramatically improved its hydrophilicity. The purified GST-P50t was tested in an enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to B. gibsoni in dogs. The ELISA with GST-P50t clearly differentiated between B. gibsoni-infected dog sera and uninfected dog sera. In addition, the ELISA detected no cross-reactivity with sera from dogs experimentally infected with the closely related parasites, B. canis canis, B. canis vogeli, and B. canis rossi. Field serum samples collected from dogs in Japan and China were examined for the diagnosis of B. gibsoni infection by using the ELISA. 14.5% (9/62), 5.8% (7/120), and 5.4% (2/37) of tested samples were positive for dogs from Okinawa, Yamaguchi, and Osaka prefectures, Japan, respectively. On the other hand, 4.8% (2/41) of tested samples were positive for dogs from Nanjing, China. These results suggest that the GST-P50t could be a reliable reagent for practical use in ELISA for the serodiagnosis of canine piroplasmosis caused by B. gibsoni. 相似文献
99.
Koumei SHIRASUNA Motozumi MATSUI Takashi SHIMIZU Akio MIYAMOTO 《Animal Science Journal》2007,78(5):460-466
The corpus luteum (CL) in the estrous cycle in the cow is a dynamic organ which has a lifespan of approximately 17–18 days. As the CL matures, the steroidogenic cells establish contact with many capillary vessels and the CL is composed of a large number of vascular endothelial cells that can account for up to 50% of the bovine CL. Furthermore, luteal cells and endothelial cells secrete several vasoactive substances such as prostaglandin F2α (PGF2α), endothelin‐1 and angiotensin II. These vasoactive substances also function in regulating progesterone secretion in an autocrine/paracrine manner in the CL. The blood vessels and endothelial cells in the CL therefore have an essential role in the luteal function in the cow. Endometrial PGF2α, the primary luteolysin in the cow, stimulates luteal vasoactive substances during luteolysis. Moreover, luteal vasoactive substances may have key roles in the regulation of luteolysis to induce vasodilatation, vasoconstriction and angiolysis. This review describes the current concept for possible roles of vasoactive substances in the luteolytic cascade within the bovine CL. 相似文献
100.
Excess calcium is known to affect the metabolism of other minerals. Calcium carbonate is the main source of calcium used in previous experiments that investigated excess calcium. Thus, it remains to be clarified whether other forms of calcium also have the same adverse effects. The effects of a high‐calcium carbonate diet or a high‐calcium citrate diet on mineral concentration in several tissues of rats were examined. Male rats aged 5 weeks were fed one of the experimental diets for 4 weeks. The control diet contained calcium as calcium carbonate at the requirement level. High‐calcium diets contained calcium carbonate or calcium citrate at a level of fivefold greater than the requirement level. In rats fed the high‐calcium diets, a similar decrease in phosphorus (in the plasma) and iron (in the testis, liver and femur) was observed. Hepatic copper increased in rats fed the high‐calcium diets, whereas renal copper decreased. Therefore, excess calcium affects these mineral metabolisms regardless of its form. In contrast, the high‐calcium carbonate diet increased femoral zinc and decreased femoral magnesium, but the high‐calcium citrate diet did not affect these minerals in the femur, which suggests that the effect of excess calcium on minerals partly depends on its form. 相似文献