全文获取类型
收费全文 | 128篇 |
免费 | 0篇 |
国内免费 | 1篇 |
专业分类
林业 | 4篇 |
农学 | 5篇 |
28篇 | |
综合类 | 1篇 |
农作物 | 4篇 |
水产渔业 | 4篇 |
畜牧兽医 | 77篇 |
园艺 | 1篇 |
植物保护 | 5篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 5篇 |
2020年 | 2篇 |
2018年 | 1篇 |
2016年 | 2篇 |
2015年 | 6篇 |
2014年 | 9篇 |
2013年 | 24篇 |
2012年 | 5篇 |
2011年 | 5篇 |
2010年 | 3篇 |
2009年 | 4篇 |
2008年 | 7篇 |
2007年 | 7篇 |
2006年 | 8篇 |
2005年 | 8篇 |
2004年 | 8篇 |
2003年 | 9篇 |
2002年 | 5篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1995年 | 1篇 |
排序方式: 共有129条查询结果,搜索用时 15 毫秒
81.
黄瓜(Cucumis sativusL.)是典型的冷敏型植物。对黄瓜来说,冷害是生产上制约其丰产、优质的主要逆境因素之一。为了掌握黄瓜耐冷性遗传规律,加快黄瓜耐冷品种的选育,本研究选取黄瓜耐冷型品系0839和低温敏感型品系B52为亲本杂交得到F1和F2,进行苗期低温鉴定和遗传分析。供试亲本的耐冷性主要受一对显性单基因控制。结合BSA(群体分离分析)和SSR分子标记,获得了与黄瓜耐冷性主效基因连锁的SSR标记。通过F2群体分析,鉴定出与耐冷性基因连锁的分子标记SSR07248,该标记与耐冷性基因间的遗传距离为32.6cM。 相似文献
82.
Tohru MATSUI 《Animal Science Journal》2002,73(1):21-28
A large amount of phosphorus (P) in corn and soybean meal is in the form of phytate that is poorly available to monogastric animals. It leads to the presence of large amounts of P in manure, which contributes to the P pollution problem. The fermentation of soybean meal with Aspergillus usamii almost completely degraded phytate and improved P availability in chicks. Although dietary yeast phytase increased P absorption and availability in pigs, its efficacy was less than that of Aspergillus niger phytase. It was suggested that the lesser efficacy of yeast phytase resulted from its lower stability against pepsin. Phytate suppresses zinc availability in monogastric animals. Zinc availability was improved by the substitution of regular soybean meal with fermented soybean meal and by the supplementation with Aspergillus niger phytase in pigs. It has been considered that phytate is easily degraded in the rumen and the availability of phytate P is high in ruminants. However, 20% of phytate in oilseed meals was not degraded in the rumen of sheep. Additionally, heating and formaldehyde treatments with oilseed meals suppressed ruminal degradation of phytate and approximately half of phytate escaped from ruminal degradation in the treated oilseed meals. 相似文献
83.
Inoue K Kabeya H Hagiya K Kosoy MY Une Y Yoshikawa Y Maruyama S 《Veterinary microbiology》2011,148(1):60-65
To clarify phylogenetic relationships and genetic diversity among Bartonella washoensis strains obtained from squirrels, multi-locus sequence analysis (MLSA) with the 16S rRNA, ftsZ, gltA, groEL, ribC, and rpoB genes was applied for 20 strains of B. washoensis isolated from five genera of squirrels (Tamias, Tamiasciurus, Glaucomys, Sciurus, and Spermophilus) within the family Sciuridae. Sequence similarities in the concatenated sequences of B. washoensis strains from squirrels of different genera ranged from 94.7% (Sciurus vs. Spermophilus) to 98.4% (Tamiasciurus vs. Glaucomys). Phylogenetic trees based on the concatenated sequences revealed that B. washoensis strains formed five distinct clades and each clade correlated with the genus of squirrel from which the strains were originally obtained. The discrimination was supported by 100% bootstrap values and posterior probabilities, respectively. These results suggest that B. washoensis strains may have co-speciated with their squirrel hosts and provide new insights into the application of the MLSA to identify sources of B. washoensis infection with accuracy. 相似文献
84.
Tani H Noda N Yamada K Kurata S Tsuneda S Hirata A Kanagawa T 《Journal of agricultural and food chemistry》2005,53(7):2535-2540
Quenching probe (QProbe) polymerase chain reaction (PCR) is a simple and cost-effective real-time PCR assay in comparison with other real-time PCR assays such as the TaqMan assay. We used QProbe-PCR to quantify genetically modified (GM) soybean (Roundup Ready soybean). We designed event-specific QProbes for Le1 (soy endogenous gene) and RRS (recombinant gene), and we quantified certified reference materials containing 0.1, 0.5, 1, 2, and 5% GM soybean. The TaqMan assay was also applied to the same samples, and the results were compared. The accuracy of QProbe-PCR was similar to that of TaqMan assay. When GM soybean content was 0.5% or more, the relative standard deviations of QProbe-PCR were less than 20%. QProbe-PCR is sensitive enough to monitor labeling systems and has acceptable levels of accuracy and precision. 相似文献
85.
Tomoaki ONODA Ryuta YAMAMOTO Kyohei SAWAMURA Yoshinobu INOUE Akira MATSUI Takeshi MIYAKE Nobuhiro HIRAI 《Journal of Equine Science》2011,22(2):37-42
Thoroughbred horses are seasonal mating animals, raised in northern regions or countries.
Foals born yearly in spring generally show a typical seasonal compensatory growth pattern,
in which their growth rate declines in the first winter and increases in the next spring.
In this study, a new empirical adjustment approach is proposed to adjust for this
compensatory growth when growth curve equations are estimated, by using 1,633 male body
weights of Thoroughbreds as an illustrating example. Based on general Richards growth
curve equation, a new growth curve equation was developed and fit to the weight-age data.
The new growth curve equation had a sigmoid sub-function that can adjust the compensatory
growth, combined with the Richards biological parameter responsible for the maturity of
animals. The unknown parameters included in the equations were estimated by SAS NLMIXED
procedure. The goodness of fit was examined by using Akaike’s Information Criterion (AIC).
The AIC values decreased from 13,053 (general Richards equation) to 12,794 (the newly
developed equation), indicating the better fit of the new equation to the weight-age data.
The shape of the growth curve was improved during the period of compensatory growth. The
proposed method is one of the useful approaches for adjusting seasonal compensatory growth
in growth curve estimations for Thoroughbreds, and for their management during the
compensatory period. Based on this approach, the optimal growth curve equations can be
estimated also for female body weight of Thoroughbreds or other growth traits affected by
seasonal compensatory growth. 相似文献
86.
In the subsurface horizons of some paddy fields at the Kojima polder, Okayama Prefecture, the authors noticed a peculiar Mn-mottling, which had not been found in the common paddy fields. Several paddy soils in varying stages of maturity were carefuIIy surveyed at the Kojima polder. The soils were tentatively named OK4, OK7, OKS, and OK9. The ages of the fields after reclamation were about 10, 100, 150, and 250 years, respectively. Undisturbed samples were collected from 3 soils (OK4, OK7, and OKS) for microscopic observation and chemical analysis. 相似文献
87.
Hidenori Wada Supamard Panichsakpatana Makoto Kimura Yasuo Takai 《Soil Science and Plant Nutrition》2013,59(3):357-365
The 4 long-term experimental plots (Umbric haplaquept) with different fertilizer treatment at Cent. Agric. Exp. Sta. in Konosu City, Saitama Prefecture, were used for the sites of investigation. The 4 plots were NF (applied with no fertilizer), IF (applied with inorganic fertilizers), GM (applied with green manure and CaCO2), and OM (applied with manure and inorganic fertilizers). Flooded water, floating weed, upper (0-2cm) and lower (2-10cm) parts of Apg horizon and rhizosphere were collected from each plot before flooding, during flooding, and after drainage. These samples were analyzed for N2-fixing activity by acetylene reduction method, pH, Eh, and contents of Fe2+, NH4 +, chlorophyll-type compounds, and water-soluble carbohydrates. The N2-fixing activity of all samples showed almost the same pattern of change with time: very low before flooding, rapidly increased after flooding, the maximum value at the maximum tillering stage of rice plant, declined afterwards and reached a very low value after drainage. Rough estimation of the “N2-fixing capacity” of each part of the paddy field revealed that the most important site of the N2 fixation was the reduced Apg horizon, that the importance of flooded water and/or the oxidized layer in the N2 fixation was rather low except in infertile soil, and that the role of rhizosphere in the N2 fixation could not be neglected also in Japan. Reduced condition and content of easily decomposable organic substances were judged to be main factors which control the N2-fixing activity in the flooded soil on the basis of correlations between the Nt-fixing activity and several analytical data of the paddy soils. 相似文献
88.
Decha Pangjai Soichi Maruyama Sumalee Boonmar Hidenori Kabeya Shingo Sato Burin Nimsuphan Wimol Petkanchanapong Wattanapong Wootta Piyada Wangroongsarb Maskiet Boonyareth Poom Preedakoon Watcharee Saisongkorh Pathom Sawanpanyalert 《Comparative immunology, microbiology and infectious diseases》2014
We investigated the prevalence of Bartonella species in 10 rodent and one shrew species in Thailand. From February 2008 to May 2010, a total of 375 small animals were captured in 9 provinces in Thailand. Bartonella strains were isolated from 57 rodents (54 from Rattus species and 3 from Bandicota indica) and one shrew (Suncus murinus) in 7 of the 9 provinces, and identified to the species level. Sequence analysis of the citrate synthase and RNA polymerase β subunit genes identified the 58 isolates from each Bartonella-positive animal as B. tribocorum in 27 (46.6%) animals, B. rattimassiliensis in 17 (29.3%) animals, B. elizabethae in 10 (17.2%) animals and B. queenslandensis in 4 (6.9%) animals. R. norvegicus, R. rattus, and Suncus murinus carried B. elizabethae, which causes endocarditis in humans. The prevalence of Bartonella bacteremic animals by province was 42.9% of the animals collected in Phang Nga, 26.8% in Chiang Rai, 20.4% in Sa Kaeo, 16.7% in Nakhon Si Thammarat, 12.0% in Surat Thani, 9.1% in Mae Hong Son and Loei Provinces. 相似文献
89.
90.
Eiko Touno Makoto Kaneko Sunao Uozumi Hidenori Kawamoto Shin Deguchi 《Animal Science Journal》2014,85(1):46-52
Twelve sheep were used in a 3 × 3 Latin square design to evaluate the effect of substituting wheat bran with forage soybean silage in the diet on apparent digestibility and nitrogen balance. Forage soybean (Glycine max (L.) Merrill) was cultivated in a no‐till, no‐herbicide cropping system with Italian ryegrass (Lolium multiflorum Lam.) as a living mulch. Forage soybean and Italian ryegrass were wilted and ensiled in round bales without additives, respectively. The experimental diets were based on corn silage supplemented with protein sources (tow silages or wheat bran). The crude protein and the acid detergent insoluble protein contents of forage soybean silage were the highest among the protein sources. The apparent digestibility of crude protein and the nitrogen balance did not significantly differ among the diets. In addition, the phytoestrogen content of forage soybean silage was below the level at which animal reproductive performance would be negatively affected. These results suggest that forage soybean silage has comparable feeding value to wheat bran, and can be given at an inclusion level of 17% (dry matter basis) as an alternative protein source to wheat bran without adverse effects on digestion or nitrogen balance in sheep fed a corn silage‐based diet. 相似文献