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171.
Fuji Shin-ichi Mochizuki Tomofumi Okuda Mitsuru Tsuda Shinya Kagiwada Satoshi Sekine Ken-Taro Ugaki Masashi Natsuaki Keiko T. Isogai Masamichi Maoka Tetsuo Takeshita Minoru Yoshikawa Nobuyuki Mise Kazuyuki Sasaya Takahide Kondo Hideki Kubota Kenji Yamaji Yasuyuki Iwanami Toru Ohshima Kazusato Kobayashi Kappei Hataya Tatsuji Sano Teruo Suzuki Nobuhiro 《Journal of General Plant Pathology》2022,88(2):105-127
Journal of General Plant Pathology - An increasing number of plant viruses and viroids have been reported from all over the world due largely to metavirogenomics approaches with technological... 相似文献
172.
We improved a multiple rapid immunofilter paper assay (multi-RIPA) to simultaneously detect three major viruses of cucumber,
Cucumber mosaic virus, Melon yellow spot virus and Zucchini yellow mosaic virus. When we increased the concentration of bovine serum albumin (BSA) in the extraction buffer, a positive band for each virus
developed in the multi-RIPA, regardless of the order that the three sensitized white latex particles were immobilized on the
strip. The increase in BSA concentration also strengthened the sensitivity of each RIPA. In addition, using a mixture of commercially
available, red and blue, sensitized latex solutions as a tracer, purple bands could be produced on the strips. Because each
virus yielded an unique color for a positive reaction, the three viruses were easy to distinguish. 相似文献
173.
Kim DH Feinbaum R Alloing G Emerson FE Garsin DA Inoue H Tanaka-Hino M Hisamoto N Matsumoto K Tan MW Ausubel FM 《Science (New York, N.Y.)》2002,297(5581):623-626
A genetic screen for Caenorhabditis elegans mutants with enhanced susceptibility to killing by Pseudomonas aeruginosa led to the identification of two genes required for pathogen resistance: sek-1, which encodes a mitogen-activated protein (MAP) kinase kinase, and nsy-1, which encodes a MAP kinase kinase kinase. RNA interference assays and biochemical analysis established that a p38 ortholog, pmk-1, functions as the downstream MAP kinase required for pathogen defense. These data suggest that this MAP kinase signaling cassette represents an ancient feature of innate immune responses in evolutionarily diverse species. 相似文献
174.
Scheschkewitz D Amii H Gornitzka H Schoeller WW Bourissou D Bertrand G 《Science (New York, N.Y.)》2002,295(5561):1880-1881
Singlet diradicals are usually not energy minima. As observed by femtosecond spectroscopy, they readily couple to form final sigma bonds. Substituent effects allow lifetimes to increase into the microsecond range. Taking advantage of the properties of hetero-elements, a diradical has been prepared that is indefinitely stable at room temperature. The availability of diradicals that can be handled under standard laboratory conditions will lead to further insight into their chemical and physical properties, raising the likelihood of practical applications, especially in the field of molecular materials such as electrical conductors and ferromagnets. 相似文献
175.
Hirofumi Furuita Hideki Tanaka Takeshi Yamamoto Nobuhiro Suzuki & Toshio Takeuchi 《Aquaculture Research》2003,34(6):461-468
This study investigated supplemental effects of vitamin A in diet on reproduction of Japanese flounder Paralichthys olivaceus (T & S). Broodstock were fed either a non‐supplemented diet (control diet; CD) or a vitamin A‐supplemented diet (supplemental diet; SD) 1 month before and during the spawning season. Both dietary groups began to spawn in mid‐January. At the beginning of spawning, no notable differences were found in spawning or egg quality between treatments. Egg production of the CD broodstock began to decrease from mid‐April and spawning stopped in late May, whereas the SD group continued to spawn until the end of June. Buoyant egg rate of the CD group was lower than that of the SD group throughout the spawning period. Hatching rate was not different between the CD and SD groups. The percentage of normal larvae in the CD group was significantly lower than in the SD group. It is concluded that a low vitamin A content in the diet has some negative effects on reproduction of flounder. The importance of supplementation of vitamin A in broodstock diet of the Japanese flounder is suggested. 相似文献
176.
Satoshi KUSUDA Hisashi HASHIKAWA Masato TAKEDA Hideki ITO Atsushi GOTO Jun OGUCHI Osamu DOI 《The Journal of reproduction and development》2013,59(3):308-313
The purposes of the present study were to clarify age- and season- related androgen
patterns, and to compare the reproductive physiology between Japanese captive koala
populations and Australian populations. To measure fecal androgens, feces were collected
from male koalas (4.2 to 13.8 years of age) kept in Japanese zoos. Fecal androgens were
extracted with methanol from the lyophilized samples and determined by enzyme immunoassay
using 4-androstene-3,17-dione antibody. Fecal androgen concentration in male koalas
increased after sexual maturation and remained relatively high until old age. In the
survey with the Japanese zoo studbook of koalas, copulation (conception) month showed a
pyramid shape with a peak in March to June (60.7%) in koalas born and reared in Japanese
zoos and from July to April with the highest concentration in September to January (69.7%)
in Australian institutes. Japanese zoo koala populations have a characteristic
physiological cycle adapted to Japan''s seasonal changes. The suitable month of year for
copulation or conception in Japan is diametrically opposed to that in Australia. Mean
fecal androgen concentrations by month in the males born and reared in Japan indicated
annual changes with the highest concentration in May and the lowest value in November.
Fecal androgen analysis may be a noninvasive alternative tool to monitor circulating
testosterone and may be helpful in understanding reproductive activity and physiology in
male koalas. 相似文献
177.
Hideki KINOSHITA Shun IMOTO Yoshihito SUDA Mitsuharu ISHIDA Masamichi WATANABE Yasushi KAWAI Haruki KITAZAWA Koh MIURA Akira HORII Tadao SAITO 《Animal Science Journal》2013,84(2):150-158
Adhesion tests are complex, time‐consuming and expensive, while the most important criterion for a probiotic lactobacilli is the ability to adhere to the human intestine. Thirty lactobacilli isolates from human intestinal tissues were measured for cell surface glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) activity using a microtiter plate screening method. GAPDH activities were detected in 21 out of 30 samples from 12 h cultures and in all samples from 18 h cultures. This suggests GAPDH is universally expressed on the bacterial cell surfaces from many lactobacilli. A statistically significant positive correlation was shown between GAPDH activity and adhesion using the BIACORE adhesion assay (P < 0.01). The new screening method using GAPDH enzymatic activity without an adhesion test may be possible due to the significant positive correlation of GAPDH activity with adhesion of lactobacilli derived from the human intestine. 相似文献
178.
Hisayo Yamane Hideki Murayama Akira Sugiura 《The Journal of Horticultural Science and Biotechnology》2013,88(5):562-567
SummaryBased on the cDNA sequences encoding sweet cherry self-incompatibility associated ribonucleases (S-RNases), a PCR-based S-allele typing system for sweet cherry cultivars has been recently developed. Using this technique, we determined S-genotypes of the three newly released Japanese cvs Kouka-Nishiki, Beni-Sayaka and Beni-Shuho and one British cv Merton Glory that was classified as a Universal Donor, which is able to be used as a pollen donor for all cultivars in pollen incompatibility groups I to XIII. Furthermore, we also determined the partial sequences of the S-RNase genes of ‘Rainier’ (S1S4)‘ and ‘Sato-Nishiki (S3S6)’,which leads to the development of a more reliable S-allele identification method of PCR-RFLP for sweet cherry cultivars. Total DNA isolated from leaves of the four cultivars along with those from ten cultivars with known S-genotypes were PCR amplified with two sets of primers that were designed from DNA sequences encoding the signal peptide (Pru-T2) and two conserved domains (Pru-C2 and Pru-C4R) of sweet cherry S-RNases. By comparing the size of PCR products on agarose gel, the 5-genotypes of ‘Kouka-Nishiki’, ‘Beni-Sayaka’, ‘Beni-Shuho’ and ‘Merton Glory’ were suggested to be S1S3, S1S6, S4S6, and S4S6, respectively. Two of these three S-genotypes (S1S6 and S4S6) were found for the first time. DNA sequencing of PCR products from S-alleles of ‘Rainier’ and ‘Sato-Nishiki’ revealed that Ban II, Nru I, Apa LI and Ava I sites, respectively, were unique in the S1-, S3-, S4- and S6- sequences flanked by Pru-T2 and Pru-C4R primers. RFLP analysis of the PCR products using these enzymes confirmed that S1-, S3-, S4- and S6-alleles of the four cultivars contained the respective restriction enzyme recognition sites. 相似文献
179.
Yusuke Komiya Wataru Mizunoya Kurumi Kajiwara Issei Yokoyama Hideki Ogasawara Keizo Arihara 《Animal Science Journal》2020,91(1)
The difference of muscle fiber type composition affects several parameters related to meat quality; however, the relationship between muscle fiber types and meat taste is unclear. To elucidate this relationship, we determined the taste of various beef samples using a taste sensor (INSENT SA402B) and analyzed its correlation with different muscle fiber type composition. We used 22 kinds of beef samples and measured nine tastes, including the relative and change of membrane potential caused by adsorption (CPA) values, using six sensors (GL1, CT0, CA0, AAE, C00, and AE1). The taste sensor analysis indicated positive value outputs for the relative C00, AAE, and GL1 values as well as for the CPA value of AAE, which corresponded to bitterness, umami, sweetness, and richness, respectively. We found significant positive correlations of the myosin heavy chain 1 (MyHC1) composition with umami taste, and with richness. This result suggests that high levels of slow MyHC1 can induce strong umami taste and richness in beef. We expect that our results will contribute to the elucidation of the relationship between muscle fiber types and meat palatability. 相似文献