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41.
42.
Spatial and temporal landscape patterns have long been recognized to influence biological processes, but these processes often
operate at scales that are difficult to study by conventional means. Inferences from genetic markers can overcome some of
these limitations. We used a landscape genetics approach to test hypotheses concerning landscape processes influencing the
demography of Lahontan cutthroat trout in a complex stream network in the Great Basin desert of the western US. Predictions
were tested with population- and individual-based analyses of microsatellite DNA variation, reflecting patterns of dispersal,
population stability, and local effective population sizes. Complementary genetic inferences suggested samples from migratory
corridors housed a mixture of fish from tributaries, as predicted based on assumed migratory life histories in those habitats.
Also as predicted, populations presumed to have greater proportions of migratory fish or from physically connected, large,
or high quality habitats had higher genetic variability and reduced genetic differentiation from other populations. Populations
thought to contain largely non-migratory individuals generally showed the opposite pattern, suggesting behavioral isolation.
Estimated effective sizes were small, and we identified significant and severe genetic bottlenecks in several populations
that were isolated, recently founded, or that inhabit streams that desiccate frequently. Overall, this work suggested that
Lahontan cutthroat trout populations in stream networks are affected by a combination of landscape and metapopulation processes.
Results also demonstrated that genetic patterns can reveal unexpected processes, even within a system that is well studied
from a conventional ecological perspective. 相似文献
43.
Williams SE Wootton P Mason HS Bould J Iles DE Riccardi D Peers C Kemp PJ 《Science (New York, N.Y.)》2004,306(5704):2093-2097
Modulation of calcium-sensitive potassium (BK) channels by oxygen is important in several mammalian tissues, and in the carotid body it is crucial to respiratory control. However, the identity of the oxygen sensor remains unknown. We demonstrate that hemoxygenase-2 (HO-2) is part of the BK channel complex and enhances channel activity in normoxia. Knockdown of HO-2 expression reduced channel activity, and carbon monoxide, a product of HO-2 activity, rescued this loss of function. Inhibition of BK channels by hypoxia was dependent on HO-2 expression and was augmented by HO-2 stimulation. Furthermore, carotid body cells demonstrated HO-2-dependent hypoxic BK channel inhibition, which indicates that HO-2 is an oxygen sensor that controls channel activity during oxygen deprivation. 相似文献
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The transmission of mucosal disease virus (MDV) from infected ewes and their lambs to susceptible sheep was investigated. MDV was recovered from the amniotic fluid of an infected pregnant ewe and from the blood, nasal swabs and urine of hairy lambs. MDV infection was transmitted either at lambing, from infective foetal fluids or lambs, or later as a result of contact with a surviving with a hairy lamb and either aborted or gave birth to an infected hairy lamb. Adult sheep and 12-months-old sheep were less readily infected than were newborn lambs. Pregnant ewes were infected by contact with a hairy lamb and either aborted or gave birth to an infected hairy lamb. A method to minimise spread of the infection in the field is suggested. 相似文献
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Edwards LJ Goodship AE Birch HL Patterson-Kane JC 《American journal of veterinary research》2005,66(4):564-568
OBJECTIVE: To determine whether specific treadmill exercise regimens would accelerate age-related changes in collagen fibril diameter distributions in the common digital extensor tendon (CDET) of the forelimbs of young Thoroughbreds. ANIMALS: 24 female Thoroughbreds. PROCEDURE: Horses were trained for 18 weeks (6 horses; short term) or 18 months (5 horses; long term) on a high-speed treadmill; 2 age-matched control groups (6 horses/group) performed walking exercise only. Horses were (mean +/- SD) 24 +/- 1 months and 39 +/- 1 months old at termination of the short-term and long-term regimens, respectively. Midmetacarpal CDET specimens were obtained and processed for transmission electron microscopy. Diameter and area of at least 1,000 collagen fibrils/specimen were measured by use of computerized image analysis. Mass-average diameter (MAD) of collagen fibrils and collagen fibril index were calculated for each horse. RESULTS: Collagen fibril MAD for the older horses was significantly less than that for the younger horses. Exercise did not significantly affect fibril diameter or distributions in either age group, and collagen fibril index did not differ significantly between groups. CONCLUSIONS AND CLINICAL RELEVANCE: Age-related reduction in collagen fibril MAD agreed with findings for other tendons and species. Training did not accelerate age-related change in the CDET in contrast to a reported decrease in collagen fibril MAD in the superficial digital flexor tendon of horses trained long term. Our results support the concept that the functionally distinct nature of the CDET and superficial digital flexor tendon in horses results in fundamentally different responses to high-speed exercise regimens. 相似文献
49.
Senior Care Guidelines Task Force AAHA Epstein M Kuehn NF Landsberg G Lascelles BD Marks SL Schaedler JM Tuzio H 《Journal of the American Animal Hospital Association》2005,41(2):81-91
This paper provides a working framework for enhancing the well-being of senior pet dogs and cats. Approaches to screening the medical status of senior pets are described in detail, with particular emphasis on establishing baseline data in healthy animals, the testing of clinically ill animals, and assessing senior pets prior to anesthesia and surgery. The management of pain and distress and the application of hospice and palliative care are addressed. Advice on ways to approach euthanasia and dealing with end-of-life issues is also provided. 相似文献
50.