Geographical distribution and prevalence of tick‐borne encephalitis virus in questing Ixodes ricinus ticks and phylogeographic structure of the Ixodes ricinus vector in Norway

The tick‐borne encephalitis virus (TBEV), a zoonotic flaviviral infection, is endemic in large parts of Norway and Eurasia. Humans are mainly infected with TBEV via bites from infected ticks. In Norway, the main geographical distribution of ticks is along the Norwegian coastline from southeast (~59°N) and up to the southern parts of Nordland County (~65°N). In this study, we collected ticks by flagging along the coast from Østfold County to Nordland County. By whole‐genome sequencing of the mitochondrial genome of Ixodes ricinus, the phylogenetic tree suggests that there is limited phylogeographic structure both in Norway and in Europe. The overall TBEV prevalence is 0.3% for nymphs and 4.3% for adults. The highest estimated TBEV prevalence in adult ticks was detected in Rogaland and Vestfold County, while for nymphs it is highest in Vestfold, Vest‐Agder and Rogaland. The present work is one of the largest studies on distribution and prevalence of TBEV in ticks in Scandinavia, showing that the virus is wider distributed in Norway than previously anticipated.     

EGFR mutations in lung cancer: correlation with clinical response to gefitinib therapy

Receptor tyrosine kinase genes were sequenced in non-small cell lung cancer (NSCLC) and matched normal tissue. Somatic mutations of the epidermal growth factor receptor gene EGFR were found in 15of 58 unselected tumors from Japan and 1 of 61 from the United States. Treatment with the EGFR kinase inhibitor gefitinib (Iressa) causes tumor regression in some patients with NSCLC, more frequently in Japan. EGFR mutations were found in additional lung cancer samples from U.S. patients who responded to gefitinib therapy and in a lung adenocarcinoma cell line that was hypersensitive to growth inhibition by gefitinib, but not in gefitinib-insensitive tumors or cell lines. These results suggest that EGFR mutations may predict sensitivity to gefitinib.     

Analysis of isomeric forms of oxidized triacylglycerols using ultra-high-performance liquid chromatography and tandem mass spectrometry

Detailed studies on the regioisomeric structures of oxidized species of triacylglycerols (TAG), formed in food during storage and processing, have not been published thus far. In this study, an analytical approach based on efficient ultra-high-performance liquid chromatographic (UHPLC) separation of different isomers of oxidized TAG species and their tandem mass spectrometric analysis was created. A linear solvent gradient based on acetonitrile and acetone was used in the UHPLC method. A novel method utilizing positive ion ESI using ammonia supplemented in the nebulizer gas was used to produce ammonium adduct ions for mass spectrometric analysis. With the UHPLC method used, different regioisomers of TAG species containing oxidized linoleic or oleic acid could be efficiently resolved. Differences in the fragmentation patterns of many of the oxidized TAG isomers could be demonstrated by the tandem mass spectrometric method. On the basis of the results, the approach enables regiospecific analysis of oxidized TAG molecules.     

Effects of poultry manure amendment on phosphorus uptake by ryegrass,soil phosphorus fractions and phosphatase activity

Poultry manure (PM) contains a large proportion of phosphorus (P) in mineral-associated forms that may not be readily available for plant uptake. In addition, PM application influences both chemical and biotic processes, and can affect the lability of native soil P. To investigate the effects of PM on soil P availability, we grew ryegrass (Lolium perenne) in greenhouse pots amended with poultry manure. Biomass was harvested at 4, 8, and 16 weeks following PM application, with soil separated into rhizosphere and bulk fractions. Soil was sequentially extracted by H₂O, 0.5 M NaHCO₃, 0.1 M NaOH, and 1 M HCl, and inorganic P (P_i) and enzymatically hydrolyzable organic P (P_oe) were quantitated. Root P concentrations were 37% higher and total P uptake 59% higher with PM application than Control. At week 16, there was 30% more labile-P_i (H₂O- plus NaHCO₃-P_i) in the rhizosphere with PM than in Control. Phosphodiesterase activity increased with PM application. Furthermore, acid phosphomonoesterase, alkaline phosphomonoesterase, and phosphodiesterase activities were all higher in the rhizosphere than in bulk soil at week 16 with PM, indicating that increased labile-P_i was due primarily to stimulation of soil phosphatases to mineralize NaOH-P_oe. Soil pH increased with PM application and plant growth, and may have promoted P availability by decreasing sorption of Al- and Fe-associated inorganic and organic phosphates. These results demonstrate that whereas PM application may initially increase NaOH and HCl-P_i, these fractions can be readily changed into labile-P and do not necessarily accumulate as stable or recalcitrant P in soil.     

Soil Morphologic Properties and Cattle Stocking Rate Affect Dynamic Soil Properties

Soil properties that influence the capacity for infiltration and moisture retention are important determinants of rangeland productivity. Monitoring effects of grazing on dynamic soil properties can assist managers with stocking rate decisions, particularly if monitoring takes into account environmental variability associated with inherent soil morphological properties. On a Pacific Northwest Bunchgrass Prairie in northeast Oregon, we applied three cattle stocking rates (0.52, 1.04, and 1.56 animal unit months · ha^?1) and an ungrazed control in a randomized complete block design for two 42-d grazing seasons and measured the change in four dynamic soil properties: soil penetration resistance, soil aggregate stability, bare ground, and herbaceous litter cover. To address apparent environmental heterogeneity within experimental units, we also utilized a categorical soil factor (termed Edaphic Habitat Types or EHT), determined by characterizing soil depth, texture, and rock fragment content at sample sites. Stocking rate did not affect extent of bare ground or soil aggregate stability. Stocking rate had a significant effect on penetration resistance, which was greatest at the high stocking rate (1.6 J · cm^?1 ± 0.1 SE) and lowest in the control (1.1 J · cm^?1 ± 0.1 SE). For litter cover, the effects of stocking rate and EHT interacted. In two rocky EHTs, litter cover was highest in the controls (60% ± 6 SE; 50% ± 3 SE) and ranged from 27% ± 3 SE to 33% ± 6 SE in the stocking rate treatments. Measures of penetration resistance, aggregate stability, and bare ground were different across EHTs regardless of stocking rate, but did not interact with stocking rate. Our study demonstrates that response of dynamic soil properties to stocking rates should be considered as a useful and accessible approach for monitoring effects of livestock management decisions on rangeland conditions.     

Transformative food systems education in a land-grant college of agriculture: the importance of learner-centered inquiries

In this paper we use a critically reflective research approach to analyze our efforts at transformative learning in food systems education in a land grant university. As a team of learners across the educational hierarchy, we apply scholarly tools to the teaching process and learning outcomes of student-centered inquiries in a food systems course. The course, an interdisciplinary, lower division undergraduate course at the University of California, Davis is part of a new undergraduate major in Sustainable Agriculture and Food Systems. We provide an overview of the course’s core elements—labs, exams, assignments, and lectures—as they relate to social constructivist learning theory and student-centered inquiries. Then, through qualitative analysis of students’ reflective essays about their learning experiences in the course, we demonstrate important transformative outcomes of student-centered inquiries: (1) most students confronted the commodity fetish and tried to reconcile tensions between what the food system is and ought to be, and (2) students repositioned themselves, their thinking, and social deliberation in relation to the food system. Students’ reflections point to the power of learning that emerges through their inquiry process, including in the field, and from critical self-reflection. We also highlight the importance of reflective essays in both reinforcing experiential learning and in helping instructors to better understand students’ learning vis-à-vis our teaching.