Experimental evidence that tryptamine alkaloids do not cause Phalaris aquatica sudden death syndrome in sheep

The acute toxicity for sheep of 3 alkaloids that occur in Phalaris acquatica was examined by intravenous and oral administration. The lowest tested dose rates that produced clinically observed signs were, for 5-methoxy dimethyltryptamine, 0.1 mg/kg body weight intravenously and 40 mg/kg orally; for gramine, 10 mg/kg intravenously and 500 mg/kg orally; and for hordenine, 20 mg/kg intravenously and 800 mg/kg orally. All induced the clinical signs observed in the nervous form of phalaris toxicity, but none induced the cardiac, sudden death, syndrome.     

Vaccine efficacy for reducing turbinate atrophy and improving growth rate in piggeries with endemic atrophic rhinitis

Two vaccines, based on formalin-killed whole cells of toxigenic Pasteurella multocida type D and Bordetella bronchiseptica combined with a partially toxoided cell extract of P multocida, were prepared with Freund's incomplete adjuvant (vaccine 1) or by alum precipitation (vaccine 2). Each was tested for safety and efficacy in reducing the severity of nasal turbinate atrophy and improving the growth rate of pigs in three Western Australian commercial piggeries with endemic atrophic rhinitis. In safety experiments with vaccine 1, no adverse clinical effects were observed in vaccinated sows or their progeny. Piglets receiving vaccine 2 showed no injection site abnormalities, pyrexia or turbinate atrophy. In field trials, vaccine 1 significantly reduced the prevalence of moderate to severe nasal turbinate atrophy (Done score 3 to 5) when used in two piggeries (A and B). Progeny from vaccinated sows in piggery B also grew significantly faster than controls. When vaccine 2 was used in piggery A at a later date and in another piggery (C), growth rate was not improved in either piggery and the prevalence of moderate to severe turbinate atrophy was reduced only in piggery C.     

Prothrombin time and activated partial thromboplastin time using a point‐of‐care analyser (Abaxis VSpro®) in Bennett's wallabies (Macropus rufogriseus)

There are few reports of coagulation times in marsupial species. Blood samples collected from 14 Bennett's wallabies (Macropus rufogriseus) under anaesthesia during routine health assessments were analysed for prothrombin time (PT) and activated partial thromboplastin time (aPTT) using a point‐of‐care analyser (POC) (Abaxis VSPro®). The wallabies had an aPTT mean of 78.09 s and median of 78.1 s. The PT for all wallabies was greater than 35 s, exceeding the longest time measured on the POC. Although PT was significantly longer, aPTT was similar to the manufacturer's domestic canine reference range.     

Afforestation effects on soil carbon stocks of low productivity grassland in New Zealand

Soil carbon stock change between two major land uses in New Zealand was measured by sampling paired plots across the boundaries of low productivity grassland and forest planted pre‐1990. The national soil carbon monitoring system uses low productivity grassland as a benchmark to evaluate soil carbon stock change for other land uses. The goal was to validate earlier estimates of the effect of pre‐1990 afforestation and to reduce their level of uncertainty. We selected a set of sites to represent the national stocks of forests planted pre‐1990. Previous studies derived estimates of the land‐use effect on soil carbon for afforestation ranging from +1.6 to ?8.5 t/ha to 30 cm depth. For all estimates, the 95% confidence interval spanned zero. Our study used nine of the previous paired‐plot sites and sampled and analysed 21 new sites. The land‐use effect of change from grassland to forest planted pre‐1990 was estimated at ?17.4 t/ha. The 95% confidence interval ranged from ?10.1 to ?24.6 t/ha and did not include zero change. The result supported the soil carbon monitoring system assumption that forests planted pre‐1990 have significantly lower soil carbon stocks than the low‐productivity‐grassland standard. Evidence of stock change occurred in depth increments to 0.2 m but with no significant change for the 0.2–0.3 m increment. This suggests that the sampling depth of 0.3 m was adequate for the estimation of soil carbon stock change.