Formation of molecular chlorine from the photolysis of ozone and aqueous sea-salt particles

Halogen atoms from the reactions of sea-salt particles may play a significant role in the marine boundary layer. Reactions of sodium chloride, the major component of sea-salt particles, with nitrogen oxides generate chlorine atom precursors. However, recent studies suggest there is an additional source of chlorine in the marine troposphere. This study shows that molecular chlorine is generated from the photolysis of ozone in the presence of sea-salt particles above their deliquescence point; this process may also occur in the ocean surface layer. Given the global distribution of ozone, this process may provide a global source of chlorine.     

A method for spatial prediction of daily soil water status for precise irrigation scheduling

Available water holding capacity (AWC) and field capacity (FC) maps have been produced using regression models of high resolution apparent electrical conductivity (EC_a) data against AWC (adj. R² = 0.76) and FC (adj. R² = 0.77). A daily time step has been added to field capacity maps to spatially predict soil water status on any day using data obtained from a wireless soil moisture sensing network which transmitted hourly logged data from embedded time domain transmission (TDT) sensors in EC_a-defined management zones. In addition, regular time domain reflectometry (TDR) monitoring of 50 positions in the study area was used to assess spatial variability within each zone and overall temporal stability of soil moisture patterns. Spatial variability of soil moisture within each zone at any one time was significant (coefficient of variation [% CV] of volumetric soil moisture content (θ) = 3-16%), while temporal stability of this pattern was moderate to strong (bivariate correlation, R = 0.52-0.95), suggesting an intrinsic soil and topographic control. Therefore, predictive ability of this method for spatial characterisation of soil water status, at this site, was limited by the ability of the sensor network to account for the spatial variability of the soil moisture pattern within each zone. Significant variability of soil moisture within each EC_a-defined zone is thought to be due to the variable nature of the young alluvial soils at this site, as well as micro-topographic effects on water movement, such as low-lying ponding areas. In summary, this paper develops a method for predicting daily soil water status in EC_a-defined zones; digital information available for uploading to a software-controlled automated variable rate irrigation system with the aim of improved water use efficiency. Accuracy of prediction is determined by the extent to which spatial variability is predicted within as well as between EC_a-defined zones.     

In-field hyperspectral proximal sensing for estimating quality parameters of mixed pasture

A study was conducted to explore the potential use of a hand-held (proximal) hyperspectral sensor equipped with a canopy pasture probe to assess a number of pasture quality parameters: crude protein (CP), acid detergent fibre (ADF), neutral detergent fibre (NDF), ash, dietary cation–anion difference (DCAD), lignin, lipid, metabolisable energy (ME) and organic matter digestibility (OMD) during the autumn season 2009. Partial least squares regression was used to develop a relationship between each of these pasture quality parameters and spectral reflectance acquired in the 500–2 400 nm range. Overall, satisfactory results were produced with high coefficients of determination (R ²), Nash–Sutcliffe efficiency (NSE) and ratio prediction to deviation (RPD). High accuracy (low root mean square error-RMSE values) for pasture quality parameters such as CP, ADF, NDF, ash, DCAD, lignin, ME and OMD was achieved; although lipid was poorly predicted. These results suggest that in situ canopy reflectance can be used to predict the pasture quality in a timely fashion so as to assist farmers in their decision making.     

Effect of Cryopreservation on the Sperm DNA Fragmentation Dynamics of the Bottlenose Dolphin (Tursiops truncatus)

Sperm DNA fragmentation is one of the major causes of infertility; the sperm chromatin dispersion test (SCDt) evaluates this parameter and offers the advantage of species‐specific validated protocol and ease of use under field conditions. The main purpose of this study was to evaluate sperm DNA fragmentation dynamics in both fresh and post‐thaw bottlenose dolphin sperm using the SCDt following different cryopreservation protocols to gain new information about the post‐thaw differential sperm DNA longevity in this species. Fresh and cryopreserved semen samples from five bottlenose dolphins were examined for sperm DNA fragmentation dynamics using the SCDt (Halomax^®). Sperm DNA fragmentation was assessed immediately at collection and following cryopreservation (T0) and then after 0.5, 1, 4, 8, 24, 48 and 72 h incubation at 37°C. Serially collected ejaculates from four dolphins were frozen using different cryopreservation protocols in a TES‐TRIS‐fructose buffer (TTF), an egg‐yolk‐free vegetable lipid LP1 buffer (LP1) and human sperm preservation medium (HSPM). Fresh ejaculated spermatozoa initially showed low levels of DNA fragmentation for up to 48 h. Lower Sperm DNA fragmentation (SDF) was found in the second fresh ejaculate compared to the first when more than one sample was collected on the same day (p < 0.05); this difference was not apparent in any other seminal characteristic. While there was no difference observed in SDF between fresh and frozen–thawed sperm using the different cryopreservation protocols immediately after thawing (T0), frozen–thawed spermatozoa incubated at 37°C showed an increase in the rate of SDF after 24 h. Sperm frozen in the LP1^? buffer had higher levels (p < 0.05) of DNA fragmentation after 24‐ and 48‐h incubation than those frozen in TTF or HSPM. No correlation was found between any seminal characteristic and DNA fragmentation in either fresh and/or frozen–thawed samples.     

Antibiotic stewardship for reptiles

This review discusses the general principles underlying responsible antibiotic usage in reptiles. Very little evidence underlies antibiotic usage in reptiles, and there are no published guidelines for responsible antibiotic usage. A literature search was performed to review the evidence for bacterial involvement in the pathology of selected common diseases of reptiles, allowing the development of recommendations for responsible antibiotic treatment of those diseases.     

Differential Rotation and Dynamics of the Solar Interior

Splitting of the sun's global oscillation frequencies by large-scale flows can be used to investigate how rotation varies with radius and latitude within the solar interior. The nearly uninterrupted observations by the Global Oscillation Network Group (GONG) yield oscillation power spectra with high duty cycles and high signal-to-noise ratios. Frequency splittings derived from GONG observations confirm that the variation of rotation rate with latitude seen at the surface carries through much of the convection zone, at the base of which is an adjustment layer leading to latitudinally independent rotation at greater depths. A distinctive shear layer just below the surface is discernible at low to mid-latitudes.     

Transformation of nitrogen and nitrous oxide emission from grassland soils as affected by compaction

Animal trampling is one of the main factors responsible for soil compaction under grazed pastures. Soil compaction is known to change the physical properties of the soil thereby affecting the transformation of nitrogen (N) and the subsequent of release of N as nitrous oxide (N₂O). The form of N source added to these compacted soils further affects N emissions. Here we determine the interactive effects of soil compaction and form of N sources (cattle urine and ammonium, nitrate and urea fertilizers) on the loss of N through N₂O emission from grassland soil. Overall, soil compaction caused a seven-fold increase in the N₂O flux, the total N₂O fluxes for the entire experimental period ranged from 2.62 to 61.74 kg N₂O-N ha⁻¹ for the compacted soil and 1.12 to 4.37 kg N₂O-N ha⁻¹ for the uncompacted soil. Among the N sources, the highest emissions were measured with nitrate application, emissions being 10 times more than those from other N sources for compacted soil, suggesting that the choice of N fertilizer can go a long way in mitigating N₂O emissions in compacted grasslands.