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Summary Dry-seeded rice (Oryza sativa L., cv. Calrose) was subjected to 4 irrigation treatments — continuous flood (CF) and sprinkler irrigation at frequencies of one (S1 W), two (S2W) and three (S3W) applications per week — commencing 37 d after 50% emergence (DAE). The amount of water applied was calculated to replace water lost by pan evaporation. Urea (120 kg N ha–1) was applied in a 1:1 split 36 and 84 DAE, and there were also unfertilized controls for each irrigation treatment. Amounts of nitrate (NO 3 ) in the soil were very low throughout the growing season in all treatments, despite regular periods of draining which lasted for up to 7 d in SlW. In all irrigation treatments, the majority of the fertilizer nitrogen (N) was located in the top 20 mm of soil. After each application of fertilizer, levels of mineral N in CF declined rapidly, while levels in S3W and S1W remained high for 1–2 weeks longer. The poor growth of sprinkler-irrigated rice was not due to lower amounts of mineral N in the soil. The greater persistence of fertilizer N in the sprinkler-irrigated treatments was probably due to reduced root activity near the soil surface because of frequent periods of soil drying in between irrigations. Net mineralization of soil N in the unfertilized sprinkler-irrigated treatments was reduced by about half compared with CF.On average, the quantity of water applied (1.2–1.4 × EP) to the sprinkler-irrigated treatments appeared to be sufficient to meet the evapotranspiration demands of the crop, except possibly around flowering time. However, the plants may have suffered from moisture stress in between irrigations. Soil matric potential data at 100 mm suggested little water stress in the sprinkler-irrigated treatments during the vegetative stage, consistent with the similar tiller and panicle densities in all irrigation treatments. However, the crop was stunted and yellow and leaf rolling was observed in the sprinkler-irrigated treatments during this period. Soil matric potential data at 100 mm indicated considerable water stress in S1W beyond the commencement of anthesis, and in S2W during grain filling, consistent with the reduced floret fertility and grain weight in those treatments.  相似文献   
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Natural spawning, early development and larviculture of the ornate goby Istigobius ornatus in captivity were studied for the first time. I. ornatus spawned 46 times from 31 October 2013 to 31 October 2014. Fecundity ranged from 246 to 10,214 eggs per clutch, with an average hatching rate of 77.8% ± 9.9% (M ± SEM). Fertilized eggs (1.31–1.54 × 0.46–0.50 mm in diameter) were adhesive demersal and oval‐shaped. Embryonic development lasted 84 hr at 27.5 ± 0.5°C. Newly hatched larvae [2.12 ± 0.04 mm in total length (TL)] transformed to the juvenile stage completely when TL was 7.79 mm. Effects of different water temperatures (24, 28 and 32°C) and salinities (10, 15, 20, 25, 30, 35 and 40 g/L) on per cent survivals (%) and survival activity indices (SAIs) were tested. Survival was not significantly different under different temperatures; SAIs was significantly higher at 28°C. Larvae showed the significantly higher survival and SAIs at salinities 10–30 g/L than at 35 and 40 g/L. Effect of different prey densities on survival was significantly higher in 7 days post hatch larvae fed 20 and 30 rotifers/ml. These findings could guide future programs in captive breeding technology development and commercial production of other marine ornamental gobies.  相似文献   
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A 60‐day feeding trial was conducted to examine the effects of different levels (0, 100, 200, 400 and 600 mg/kg) of antimicrobial peptides on growth, protease activity of foregut, the morphology of foregut villi and related genes mRNA expression level in the common carp (Cyprinus carpio). The results showed that the feed of antimicrobial peptides promote common carp growth, and the optimal dosage of antimicrobial peptides is 200–333 mg/kg in the common carp feed. The protease activity of 200 and 400 mg/kg groups were significantly higher than the control and other groups (p < 0.05). The foregut villus height with 100, 200 and 400 mg/kg antimicrobial peptide groups were significantly higher than control group (p < 0.05). The crypt depth of 200 and 400 mg/kg antimicrobial peptide groups were significantly lower than control group (p < 0.05). The ratio of villus height and crypt depth of 100, 200 and 400 mg/kg antimicrobial peptide groups were significantly higher than control group (p < 0.05). The ratio with 600 mg/kg group was significantly lower than the control group (p < 0.05). The IGF‐I gene expression level of 200 mg/kg and 400 mg/kg groups were significantly higher than the control group and 600 mg/kg group (p < 0.05). The IL‐1β gene expression level of 100 mg/kg and 200 mg/kg groups were significantly higher than the control group (p < 0.05). These results indicated up‐regulation of growth and immune related genes in antimicrobial peptides fed common carp. Correlation analysis showed that IGF‐I mRNA and IL‐1β mRNA were positively correlated with SGR. IL‐1β mRNA and FCR were significantly negative correlated. It indicated that growth and immune gene common regulated the growth of the carp under antimicrobial peptides intervention. In conclusion, antimicrobial peptides can improve growth and related genes mRNA expression in the common carp. Further studies using molecular biological technique or immunologic methods are required to conclude that antimicrobial peptides are beneficial in common carp.  相似文献   
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