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61.
Katy Webb Colin Barker Tihana Harrison Zoe Heather Karen F. Steward Carl Robinson J. Richard Newton Andrew S. Waller 《Veterinary journal (London, England : 1997)》2013,195(3):300-304
Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2 h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria. 相似文献
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64.
David Eshar Bridget Lee-Chow Heather J. Chalmers 《The Canadian veterinary journal. La revue veterinaire canadienne》2013,54(12):1142-1145
Severe hydroureter and hydronephrosis secondary to ureteral obstruction by calculus were present in a guinea pig. A palliative ultrasound-guided percutaneous antegrade hydropropulsion was performed under general anesthesia to relieve the ureteral obstruction and the associated clinical signs. We describe the technique and the considerations for its potential application in similar cases. 相似文献
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66.
Antognoli MC Garry FB Hirst HL Lombard JE Dennis MM Gould DH Salman MD 《Veterinary microbiology》2008,127(3-4):300-308
Mycobacterium avium subspecies paratuberculosis (MAP) disseminated infection in dairy cattle affects animal health and productivity and is also a potential public health concern. The study objectives were to characterize MAP disseminated infection in dairy cattle and to determine the role of antemortem tests in detecting cattle with disseminated infection. Forty culled dairy cows representing a variety of serum enzyme-linked immunosorbent assay (ELISA) results and body conditions were selected for the study. The physical condition of the cows was assessed via clinical examination prior to euthanasia and blood and feces were collected and tested by serum ELISA and fecal culture, respectively. Fifteen tissues were aseptically collected from each cow during necropsy and cultured for isolation of MAP. Disseminated infection was diagnosed when MAP was isolated in tissues other than the intestines or their associated lymph nodes (LNs) and was distinguished from infection found only in the gastrointestinal tissues and from absence of infection. Of the 40 cows in the study, 21 had MAP disseminated infection. Results showed that 57% (12/21) of cows with disseminated infection had average to heavy body condition and no diarrhea. Cows with disseminated infection had no to minimal gross pathologic evidence of infection in 37% (8/21) of cases. Only 76% (16/21) of cows with disseminated infection had positive historical ELISA results and only 62% (13/21) had a positive ELISA at slaughter. Thus, antemortem evidence of MAP infection was lacking in a high proportion of cows where MAP disseminated infection was confirmed. 相似文献
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68.
Drygas KA Taylor R Sidebotham CG Hugate RR McAlexander H 《Veterinary surgery : VS》2008,37(4):322-327
Objective— To report surgical planning, technique, and long-term outcome of custom transcutaneous tibial implants used to restore ambulation after bilateral pelvic limb amputation in a dog.
Study Design— Case report.
Animals— A 4-year-old, 25.5 kg, female spayed, Siberian Husky.
Methods— Computed tomographic scans of the pelvic limbs were used to build sterolithographic models of the remaining tibia after bilateral amputation of the distal aspect of the tibiae. The sterolithographic models facilitated fabrication of implants that would replace the missing distal segments of the tibiae. Custom implants were surgically placed in both limbs.
Results— Assisted ambulation was restored immediately postoperatively and unassisted locomotion occurred at 7 days. At 14 months, aseptic loosening of the right implant occurred, it was removed and a 2nd transcutaneous implant was fabricated and implanted. At 26 months after initial surgery and 17 months after revision of the right implant, function is restored at a walk, trot, and run.
Conclusion— Based on over a 2-year follow-up, transcutaneous tibial implants allowed for restoration of locomotion.
Clinical Relevance— Transcutaneous tibial implants offer a potentially viable treatment option for restoring ambulation after amputation of the distal aspect of the tibiae in the dog. 相似文献
Study Design— Case report.
Animals— A 4-year-old, 25.5 kg, female spayed, Siberian Husky.
Methods— Computed tomographic scans of the pelvic limbs were used to build sterolithographic models of the remaining tibia after bilateral amputation of the distal aspect of the tibiae. The sterolithographic models facilitated fabrication of implants that would replace the missing distal segments of the tibiae. Custom implants were surgically placed in both limbs.
Results— Assisted ambulation was restored immediately postoperatively and unassisted locomotion occurred at 7 days. At 14 months, aseptic loosening of the right implant occurred, it was removed and a 2nd transcutaneous implant was fabricated and implanted. At 26 months after initial surgery and 17 months after revision of the right implant, function is restored at a walk, trot, and run.
Conclusion— Based on over a 2-year follow-up, transcutaneous tibial implants allowed for restoration of locomotion.
Clinical Relevance— Transcutaneous tibial implants offer a potentially viable treatment option for restoring ambulation after amputation of the distal aspect of the tibiae in the dog. 相似文献
69.
Stockdale HD Dillon AR Newton JC Bird RC Bondurant RH Deinnocentes P Barney S Bulter J Land T Spencer JA Lindsay DS Blagburn BL 《Veterinary parasitology》2008,154(1-2):156-161
Tritrichomonas foetus is recognized as the causative agent of venereal trichomoniasis in cattle. It is characterized by embryonic and early fetal death and post-coital pyometra, and feline trichomoniasis, manifest as chronic, large bowel diarrhea. Many of the infected cats are less than 2 years old and specific routes of transmission remain unknown. We recently demonstrated that feline isolates of T. foetus can successfully infect heifers, resulting in pathologic changes similar, but not identical to those previously reported as representative of bovine trichomoniasis. In this study, we experimentally infected six cats less than 1 year of age with a bovine (D-1) isolate of T. foetus and one cat with a feline (AUTf-1) isolate of T. foetus. Within 2 weeks, the cat infected with the feline (AUTf-1) isolate was culture positive for trichomonads in weekly fecal samples. At the end of 5 weeks, only one cat infected with the bovine (D-1) isolate was fecal culture positive for trichomonads. At necropsy, the intestine of each cat was removed and divided into five sections (ileum, cecum, anterior, medial and posterior colon). Contents from each section were collected and cultured. The cat infected with the feline (AUTf-1) isolate was culture positive in the ileum, cecum, medial and posterior colon. Two cats infected with the bovine (D-1) isolate were culture positive in the cecum only. Additionally, each intestinal section was submitted to a pathologist for histopathological examination. The combined results indicate that there are demonstrable differences between the feline (AUTf-1) and bovine (D-1) isolates regarding their infectivity in cats. 相似文献
70.
Briana D. Hamamoto‐Hardman Eugene P. Steffey Daniel Weiner Daniel S. McKemie Philip Kass Heather K. Knych 《Journal of veterinary pharmacology and therapeutics》2019,42(4):401-410
The objective of the current study was to describe and characterize the pharmacokinetics and selected pharmacodynamic effects of morphine and its two major metabolites in horses following several doses of morphine. A total of ten horses were administered a single intravenous dose of morphine: 0.05, 0.1, 0.2, or 0.5 mg/kg, or saline control. Blood samples were collected up to 72 hr, analyzed for morphine, and metabolites by LC/MS/MS, and pharmacokinetic parameters were determined. Step count, heart rate and rhythm, gastrointestinal borborygmi, fecal output, packed cell volume, and total protein were also assessed. Morphine‐3 glucuronide (M3G) was the predominant metabolite detected, with concentrations exceeding those of morphine‐6 glucuronide (M6G) at all time points. Maximal concentrations of M3G and M6G ranged from 55.1 to 504 and 6.2 to 28.4 ng/ml, respectively, across dose groups. The initial assessment of morphine pharmacokinetics was done using noncompartmental analysis (NCA). The volume of distribution at steady‐state and systemic clearance ranged from 9.40 to 16.9 L/kg and 23.3 to 32.4 ml min?1 kg?1, respectively. Adverse effects included signs of decreased gastrointestinal motility and increased central nervous excitation. There was a correlation between increasing doses of morphine, increases in M3G concentrations, and adverse effects. Findings from this study support direct administration of purified M3G and M6G to horses to better characterize the pharmacokinetics of morphine and its metabolites and to assess pharmacodynamic activity of these metabolites. 相似文献