Comparison of the efficacy of macrocyclic lactone anthelmintics,either singly or in combination with other anthelmintic(s), in nine beef herds in southern NSW

Anthelmintic resistance (AR) is a well-recognized challenge in farmed ruminants. The use of anthelmintics in combination is one of the strategies recommended to slow the rate of AR development. Two studies were undertaken in 2017 and 2019 to assess the efficacy of single-dose macrocyclic lactone (ML) anthelmintic and ML combination drenches. In total, 11 Faecal Egg Count Reduction Trials (FECRTs) were set up in 10 different beef herds, with results available from 10 of those FECRTs (9 herds). AR to a single ML anthelmintic was detected in all 9 herds, with resistance to Cooperia and Haemonchus spp on 9 farms, and resistance to Ostertagia and Trichostrongylus spp on 2 farms. In contrast, for the ML combination anthelmintics, all FECRTs resulted in efficacies of 99%–100%. The results suggest that cattle producers should strongly consider using combination drenches in their herds in preference to single actives.     

The correlation of genetic diversity and geographic distribution of Fusarium graminearum in North part of Iran

Fusarium Head Blight (FHB), caused mainly by Fusarium graminearum, is one of the most notorious diseases in North part of Iran. Due to lack of sufficient information regarding genetic diversity of FHB, the correlation between genetic diversity and geographic distribution of 52 isolates that collected from infected wheat kernels from four main grown region of North part of Iran were analyzed with 10 simple Sequence Repeat (SSR) primers and 15 Random Amplified Polymorphic DNA (RAPD) primers. The un-weighed pair group method using arithmetic means (UPGMA) was used and a dendrogram was constructed based on Jaccard's similarity coefficient. Principal Component Analysis (PCA) of SSR and RAPD markers revealed that the first two factors accounted for 51 and 41% of the total variance, respectively. SSR and RAPD data classified isolates into four and seven groups, respectively. SSR analysis showed that there was a correlation between genetic clusters of isolates and geographical origin in F. graminearum. However, results of cluster analysis using RAPD data didn't show any relation with geographical distribution but the results of this analysis confirmed a genetic variation among all isolates.     

Cover‐crop seeding‐date influence on fall nitrogen recovery

Planting cover crops after corn‐silage harvest could have a critical role in the recovery of residual N and N from fall‐applied manure, which would otherwise be lost to the environment. Experiments were conducted at the University of Massachusetts Research Farm during the 2004–2006 growing seasons. Treatments consisted of oat and winter rye cover crops, and no cover crop, and four cover‐crop dates of planting. The earliest planting dates of oat and winter rye produced the maximum biomass yield and resulted in the highest nitrate accumulation in both cover‐crop species. The average nitrate accumulation for the 3 years in winter rye and oat at the earliest time of planting was 60 and 48 kg ha^–1, respectively. In 2004 where the residual N level was high, winter rye accumulated 119 kg nitrate ha^–1. While initially soil N levels were relatively high in early September they were almost zero at all sampling depths in all plots with and without cover crops later in the fall before the ground was frozen. However, in plots with cover crops, nitrate was accumulated in the cover‐crop tissue, whereas in plots with no cover crop the nitrate was lost to the environment mainly through leaching. The seeding date of cover crops influenced the contribution of N available to the subsequent crop. Corn plants with no added fertilizer, yielded 41% and 34% more silage when planted after oat and rye, respectively, compared with the no–cover crop treatment. Corn‐silage yield decreased linearly when planting of cover crops was delayed from early September to early or mid‐October. Corn‐ear yield was influenced more than silage by the species of cover crop and planting date. Similar to corn silage, ear yield was higher when corn was planted after oat. This could be attributed in part to the winter‐kill of oat, giving it more time to decompose in the soil and subsequent greater release of N, while the rapidly increasing C : N ratio of rye can lessen availability to corn plants. Early plantings of cover crops increased corn‐ear yield up to 59% compared with corn‐ear yield planted after no cover crop.     

Trichomonas gallinae in budgerigars and columbid birds in Perth, Western Australia

Objective To estimate the prevalence of infection with Trichomonas gallinae and other parasites of the alimentary tract in psittacine and columbid birds in Perth and to determine in vitro the effectiveness of drugs commonly recommended for treating trichomoniasis.
Design and procedures Samples of crop contents were collected from aviary flocks of budgerigars (Melopsittacus undulatus) and other psittacine and columbid birds in both private and commercial collections in Perth. Similar samples from wild Senegal doves (Streptopelia senegalensis) also were collected. Crop contents were examined and cultured for Trichomonas gallinae and in vitro studies were conducted on the susceptibility of isolates to several drugs used commonly. Other parasites also were detected by faecal examination and/or necropsy.
Results T gallinae was recovered from birds in 1 of 13 private collections of budgerigars (2/289 birds in total). Direct wetmount examination of crop fluid identified 36.4% of samples at four commercial bird dealers which were later determined by culture to contain T gallinae . The prevalence of T gallinae infection ranged from 0 to 11.4% in budgerigars. The prevalence of T gallinae infection in wild Senegal doves was 46% and from one flock of racing pigeons was 59%. The in vitro minimum lethal concentrations of metronidazole, dimetridazole and ronidazole ranged from 40 to 96, 30 to 80 and 40 to 92 μg/mL respectively for six isolates of T gallinae . Other alimentary parasites detected during the survey included Spironucleus sp (syn Hexamita sp), coccidia, Ascaridia platycerci and Raillietina sp.
Conclusions Thirteen budgerigar flocks belonging to members of avicultural societies in Perth had a low prevalence of trichomoniasis and other parasitic infections. The dose rate currently recommended for ronidazole may not result in complete protozoacidal activity against T gallinae infection.     

Clams As recorders of ocean ridge volcanism and hydrothermal vent field activity

The clam Calyptogena magnifica lives at abyssal depths in association with hydrothermal venting on midocean ridges. Analysis of strontium/calcium ratios in C. magnifica shells provides a temperature proxy with submonthly time resolution. A 21-year strontium/calcium record of two clams from 9 degrees50'N on the East Pacific Rise captures the known 1991 and 1992 eruptive events, documents several additional events between 1992 and 1996, and demonstrates the absence of major hydrothermal episodes during the period 1974 to 1991. These clam archives can increase our understanding of the thermal and chemical history of midocean ridge hydrothermal and volcanic activity on decadal time scales.     

Effect of thinning on growth of Acer velutinum Boiss. in northern forests of Iran

It has become necessary to develop stands of fast growing endemic species in the Caspian forests because of their reduction in area and the domestic demand of timber processing industries in Iran. Acer velutinum, an en- demic species in Iran, is often planted in monocultures or in a mixture with other endemic species. Our study was con- ducted on A. velutinum planted in Berenjestanak （control plots） and Chaibagh （thinned plots） in northern Iran in 1991. A randomized sampling method with circular sampling plots of 100 m2 was used to measure diameter and height of trees. The results show that the diameter of trees in thinned stands was 19.98 cm and in the control stands 13.84 cm, while the mean heights were 18.33 and 17.46 m, respectively. The mean basal area in thinned stands was 30.74 m2 and in the control 15.70 m2 per ha. The results of Student＇s t tests indicate that there are significant differences at a probability level of 99.9% between the two variously treated stands in diameter （d）, height （h）, basal area, volume growth and the stabil- ity coefficient. We conclude that tending operations with suitable intensity in different time periods are recommended to obtain stable stands.