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81.
Matsuo S Hayashi S Watanabe T Adachi K Miyoshi A Kato A Suzuki M 《Journal of toxicologic pathology》2009,22(4):289-292
Emphysematous cystitis is a rare disorder caused by bacterial infection and characterized by gas accumulation within the bladder wall with cyst formation. This report describes the histopathological characteristics of emphysematous cystitis found in a diabetic female beagle induced by streptozotocin and alloxan. Macroscopically, multiple cyst-like structures were observed on the cut surface of the urinary mucosa. During fixation, small specimens cut from the mucosa floated on the surface of the fixative solution. Histopathologically, multiple cysts were lined with a single layer of flattened cells found to be immunohistochemically positive for vimentin, partially positive for α-smooth muscle actin or macrophage scavenger receptor, class A, and thought to be myofibroblasts, fibroblasts or macrophages. Multinucleated giant cells were observed around the cysts, and gram-negative short bacilli were observed in the lumen of the urinary bladder. From these findings, this case was diagnosed as emphysematous cystitis. 相似文献
82.
The body size of juvenile fish is often used as an index of growth rate, which in turn is influenced by local habitat conditions. We evaluated the size and origin of juvenile Chum salmon Oncorhynchus keta in the coastal areas of three regions (Atsuta, Shari, and Konbumori) of Hokkaido, northern Japan. The origin of the individuals in these communities differed between Konbumori and the other two regions. The former contained juveniles that originated from both the nearest stocked river and other rivers that were outside the area of interest. Conversely, the communities in Atsuta and Shari consisted exclusively of fish from the nearest stocked river. Moreover, the juveniles in Konbumori were larger than those in Atsuta and Shari. The results of our otolith analysis suggest that the larger size of the fish in Konbumori was due to the immigration of large individuals from natal rivers at distant locations. Thus, immigrants were likely to enlarge their body size composition in the area of interest. In summary, if the distance from a natal river is adjusted and daily growth is taken into account, body size can be used as an indicator of growth performance in coastal juvenile chum salmon. 相似文献
83.
Ruihua Li Kohji Takahashi Tomohiko Hori Akane Torita Yasushi Hasegawa 《Fisheries Science》2012,78(6):1315-1320
Elastomeric proteins occur in the shell ligament of bivalve molluscan shellfish. A newly identified protein named ligament protein-1, with a molecular mass of approximately 12 kDa, was extracted from the shell ligament of the scallop, Patinopecten yessoensis, with 70 % formic acid, and purified by gel filtration column chromatography and reverse-phase C18 column chromatography. Although the amino acid composition of this protein differs from that of the known shell ligament protein abductin, the partial amino acid sequences are similar to those of abductin. CD spectra and FT–IR spectra analyses showed that ligament protein-1 retains its secondary structures of α-helix, β-turn, and β-sheets. To the best of our knowledge, this is the first study on protein purification from the shell ligament of a bivalve molluscan shell. 相似文献
84.
Comparisons among MRI signs,apparent diffusion coefficient,and fractional anisotropy in dogs with a solitary intracranial meningioma or histiocytic sarcoma
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Masae Wada Daisuke Hasegawa Yuji Hamamoto Yoshihiko Yu Aki Fujiwara‐Igarashi Michio Fujita 《Veterinary radiology & ultrasound》2017,58(4):422-432
Although MRI has become widely used in small animal practice, little is known about the validity of advanced MRI techniques such as diffusion‐weighted imaging and diffusion tensor imaging. The aim of this retrospective analytical observational study was to investigate the characteristics of diffusion parameters, that is the apparent diffusion coefficient and fractional anisotropy, in dogs with a solitary intracranial meningioma or histiocytic sarcoma. Dogs were included based on the performance of diffusion MRI and histological confirmation. Statistical analyses were performed to compare apparent diffusion coefficient and fractional anisotropy for the two types of tumor in the intra‐ and peritumoral regions. Eleven cases with meningioma and six with histiocytic sarcoma satisfied the inclusion criteria. Significant differences in apparent diffusion coefficient value (× 10?3 mm2/s) between meningioma vs. histiocytic sarcoma were recognized in intratumoral small (1.07 vs. 0.76) and large (1.04 vs. 0.77) regions of interest, in the peritumoral margin (0.93 vs. 1.08), and in the T2 high region (1.21 vs. 1.41). Significant differences in fractional anisotropy values were found in the peritumoral margin (0.29 vs. 0.24) and the T2 high region (0.24 vs. 0.17). The current study identified differences in measurements of apparent diffusion coefficient and fractional anisotropy for meningioma and histiocytic sarcoma in a small sample of dogs. In addition, we observed that all cases of intracranial histiocytic sarcoma showed leptomeningeal enhancement and/or mass formation invading into the sulci in the contrast study. Future studies are needed to determine the sensitivity of these imaging characteristics for differentiating between these tumor types. 相似文献
85.
Usui R Usui R Fukuda M Fukui E Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1249-1252
Otitis externa in 27 toy poodles and 40 miniature dachshunds was treated using a video otoscope. A distinct concavity (external tympanic concavity) was observed at the junction between the ventral part of the external surface of the tympanum and the ear canal to which a considerable amount of hair and debris had adhered. All hair and debris adhering to the external tympanic concavity were removed, and systemic antibiotic and antifungal agents were administered, after which all of the dogs recovered. The pattern of hair growth observed in the external tympanic concavity could be characterized according to the breed of dog. All of the toy poodles presented with curly hairs, while the miniature dachshunds had upright or flat-lying hairs. 相似文献
86.
Takasawa K Kano R Maruyama H Hasegawa A Kamata H 《Veterinary immunology and immunopathology》2011,143(1-2):167-169
Lipid A, the active component of lipopolysaccharide (LPS), exists in the outer membrane of Gram-negative bacteria and binds to the Toll-like receptor 4 (TLR4) and MD-2 complex. On the other hand, the synthetic precursor of Escherichia coli lipid A, tetraacylated lipid IVa, is an agonist for TLR4 and MD-2 complex in murine, equine and feline cells but is an antagonist for lipid A in human cells. The aim of the study was to examine the function of canine Toll-like receptor 4 (TLR4) and MD-2 complex on canine blood mononuclear cells (BMC), by analyzing lipid A- or lipid IVa-induction of TNF-α production from these cells in order to understand canine innate immune system. After 5-h culture of canine BMC with lipid A (lipid A culture) or lipid IVa (lipid IVa culture), the TNF-α, as determined by ELISA, had increased in the supernatants of the lipid A cultures in a dose-dependent manner, whereas the TNF-α was undetectable in supernatant of lipid IVa-treated cultures. The TNF-α was statistically significantly different between the lipid A and lipid IVa cultures (100 and 1000 ng/ml). TNF-α production from canine BMC was inhibited, in a lipid IVa-dose-dependent manner, when the BMC were pre-cultured with lipid IVa for 60 min and then cultured with lipid A for 5h, while in control BMC cultures production if TNF-α was unchanged. These results indicate that the TNF-α production stimulated by lipid A was competed out by pre-exposing the BMC to lipid IVa. Thus, lipid A is an agonist for TNF-α production in canine BMC, whereas lipid IVa appears to be an antagonist against this lipid A stimulation of canine BMC. 相似文献
87.
Takehiro Hachiken Kei Sato Takahiro Hasegawa Katsuyuki Ichitani Makoto Kawase Kenji Fukunaga 《Genetic Resources and Crop Evolution》2013,60(4):1559-1570
To elucidate diversity and evolution of the Waxy gene in foxtail millet, Setaria italica, we analyzed sequence polymorphism of Waxy gene in 83 foxtail millet landraces collected from various regions covering the entire geographical distribution of this millet in Europe and Asia. We found a unique geographic distribution pattern at the sequence level of gene haplotypes and also found a large diversity in East Asian landraces. We also found a higher degree of genetic polymorphism in a non-waxy phenotype than in other low amylose types, supporting the hypothesis that low amylose types recently originated from non-waxy type. 相似文献
88.
C.K. Morikawa M. Saigusa H. Nakanishi N.K. Nishizawa K. Hasegawa S. Mori 《Soil Science and Plant Nutrition》2013,59(7):1013-1021
Abstract Co-situs is the placement with one application of a sufficient amount of controlled-release fertilizer for an entire growing season at any site, together with seeds or seedlings, without causing fertilizer salt injury. An experiment was conducted to find an efficient method for ameliorating Fe deficiency in two rice cultivars (cv. Tsukinohikari and cv. Sasanishiki) grown in a calcareous soil (pH 9.2, CaCO3 384 g kg?1), which was poor in organic matter (0.1 g kg?1) and available Fe (3.0 μg g?1 soil). The field treatments consisted of co-situs application of the following fertilizers: 1) controlled-release NPK fertilizer (CRF-NPK) containing no micronutrients; 2) controlled-release NPK fertilizer containing micronutrients (CRF-M1); and 3) controlled-release NPK fertilizer containing micronutrients (CRF-M2). The main difference between CRF-M1 and CRF-M2 was that the former had larger granules than the latter. All the fertilizers were placed in contact with the roots of rice seedlings at transplanting time. Plants in the CRF-M1 and CRF-M2 treatments had similar lengths, number of stems, leaf age, and leaf color (SPAR value) during the cultivation period. By contrast, plants from the CRF-NPK treatments grew poorly, showed severe chlorosis symptoms of Fe deficiency, and all died on 30 DAT. Plants of both cultivars accumulated more macroand micronutrients with the CRF-M2 treatment than with the CRF-M1 treatment. The grain yield of cv. Tsukinohikari was 0.0, 1,910, and 2,160 kg ha?1 for the CRF-NPK, CRF-M1, and CRF-M2 treatments, respectively, and 0.0, 2,490, and 2,860 kg ha?1 for the same treatments for cv. Nihonbare. Chlorosis due to iron deficiency was successfully ameliorated and world-average grain yields were obtained with the co-sites application of both controlled-release fertilizers. 相似文献
89.
Junki Ito Shigeki Hasegawa Kounosuke Fujita Shizuhiko Ogasawara Tamio Fujiwara 《Soil Science and Plant Nutrition》2013,59(2):385-393
Six year-old Japanese pear (Pyrus seratina Reheder cv. Kosui) trees grafted on P. serotina cv. Nihonyamanashi were grown in containers filled with Granite Regosol under glasshouse conditions. At different stages of fruit growth, pear trees were exposed to an elevated CO2 concentration (130 Pa CO2 ) along with a control (35 Pa CO2). For one group of plants, CO2 enrichment was applied for 79 d from 52 d after full bloom (DAB) to fruit maturity (long-term CO2 enrichment) and for another group the same treatment was applied for 35 d from 96 DAB to fruit maturity (short-term CO2 enrichment). The effects of the elevated CO2 concentration on vegetative growth, mineral contents, and fruit production and quality were examined. Long-term CO2 enrichment enhanced vegetative growth, without any significant effect on the mineral contents in either flower bud or fruit except for a remarkable increase in the K content. Long-term CO2 enrichment increased the fruit size and fresh weight, but had no significant effect on the fruit quality. On the other hand, the short-term CO2 enrichment did not induce any significant change in the fruit size but increased the fruit sugar concentration. Along with the reduction of the sorbitol concentration in fruit, the fructose and sucrose concentrations increased and these changes occurred earlier at elevated CO2 than at ambient CO2 concentrations. From these results, we concluded that the effect of CO2 enrichment on fruit growth varies depending upon the growth stages of fruit: during the initial and fruitlet stages when fruit expansion occurs, CO2 enrichment increases the fruit size, whereas, during maturation when fruit expansion has slowed down and sugar accumulation in fruit is active, it increases the fruit sugar concentration. 相似文献
90.
Tomoyuki Makino Syuichi Hasegawa Yasuhiro Sakurai Satoshi Ohno Hirokatsu Utagawa Yuji Maejima 《Soil Science and Plant Nutrition》2013,59(3):581-590
Abstract The changes in the exchangeable Mn (Ex-Mn), exchangeable Co (Ex-Co), and exchangeable Cu (Ex-Cu) contents by air-drying of soils were reported in our previous paper. The drying effect, however, was not analyzed in the field. The objective of this study was to investigate the influence of soil-drying under field conditions on Ex-Mn, Ex-Co, and Ex-Cu levels. Two paddy soils (Tsukuba and Yawara) were collected at four different soil depths (0?1, 1?5, 5?10, and 10?20 cm) under three different soil moisture conditions i.e., after harvest, after successive sunny days and after rainy days. After sunny days, Ex-Mn and Ex-Co contents in the surface soil layer markedly increased and drastically decreased after rainfall, while they remained almost unchanged in the lower soil layers. Ex-Cu contents slightly increased in the surface soil layer after sunny days. A similar tendency was observed in soil column experiments. The contents of Ex-Mn, Ex-Co, and Ex-Cu increased 12, 12, and 2 times in the upper layer of soil columns that had been sampled from the Yawara field and stored in a dark room for 8 d. The increase of the Ex-Mn and Ex-Co contents in the upper layer could not be explained by the capillary rise of soil water from the lower layers of the columns. In a soil-drying experiment under laboratory conditions, Ex-Mn and Ex-Co contents began to increase when the water content of the Yawara soil was lower than 0.09?0.13 kg kg?1. The water content of the surface soil of the Yawara field after sunny days was 0.08 kg kg?1. Ex-Cu content did not change apprecially with the water content. It is concluded that the increase of the Ex-Mn and Ex-Co levels in field surface soils after sunny days can be ascribed to soil-drying. Some of the excess Ex-Mn and Ex-Co is expected to leach down into lower layers due to rain, which may contribute to plant uptake of Mn and Co. 相似文献