Photoperiod induced off-season spawning of pink salmon (Oncorhynchus gorbuscha)

Four groups of pink salmon, which had been reared under artificial light, became sexually mature and produced viable gametes: 59 days prior to, and 19 – 32 days, 115 days and 220 days after their expected date of reaching sexual maturity. Altered times of sexual maturation were obtained by accelerating, leaving unchanged, or decelerating the rate of change of photoperiod which each group of fish would normally receive during its first year of life. All groups of fish were exposed to a normal rate of change in photoperiod during their final year of life. Mean fecundity was reduced from the 800–2000 ova observed in wild stocks, and ranged from 629 for the 59 day advanced fish, to 862 for the 115 day delayed fish. Egg mortality during the period from fertilization to eyeing was much greater in the three groups of fish subjected to accelerated or decelerated rates of change in photoperiod than in the fish subjected to the normal rate of change in photoperiod. Some of the progeny of the 220 day delayed fish, which were reared under artificial light with the normal rate of change in photoperiod set 220 days out-of-phase, became sexually mature 2 years after they had begun life as fertilized eggs.     

The wildlife preferences shown by countryside visitors

This study presents the data from part of a questionnaire survey in the Dalby Forest Area, Yorkshire, England. Of nearly 200 species mentioned, visitors hoped prior to their visit that they would see roe deer, badgers, foxes, squirrels, rabbits, pheasants, woodpeckers, kingfishers and adders, but the survey indicated that what they had in fact seen was very different. The number of times that visitors mentioned five groups of wildlife (mammals, birds, plants, trees and insects) altered with four different questions used, indicating that the degree of interaction that a visitor had with these groups varied at different physical, aesthetic and emotional levels.     

Colorimetric determination of arprinocid in feed.

An analytical method has been developed for the determination of arprinocid (9-(2-chloro-6-fluorophenylmethyl)-9H-purin-6-amine) in feed, based upon measurement of the absorbance of the diazo chromophore formed from a product of zinc reduction of the drug in acidic solution. The analyte is extracted from the feed into chloroform in the presence of a pH 7 phosphate buffer and isolated by adsorption chromatography on alumina, followed by partitioning between hexane and 0.15M HCl. The reduction product in the aqueous phase is then treated for colorimetric measurement. This procedure has been applied to determining 0.0010--0.0080% arprinocid in feed with a precision of less than 5% relative standard deviation near the middle of this concentration range. Of 32 feed additives examined, only zoalene and sulfamethazine were serious interferences. A study and discussion of several factors, e.g., reaction time, pH, and amount of zinc metal, that affect the analytical reactions are also included.     

Gas-liquid chromatographic determination of pentachlorophenol in milk

A gas-liquid chromatographic (GLC) method developed by other workers for determining pentachlorophenol (PCP) in water has been adapted for determining PCP in raw and homogenized milk. PCP is extracted from milk with benzene and from the benzene into a potassium carbonate solution. Acetic anhydride is added to the aqueous solution to form PCP acetate, which is extracted into hexane and then determined by electron capture GLC. Duplicate determinations of PCP in milk fortified at levels of 0.02, 0.2, and 2.0 ppm gave respective average recoveries of 80.0, 87.2, and 85.0%. PCP levels as low as 0.005 ppm can be detected in 20 g milk.     

Morphology,composition and age of a buried paleosol,front range,Colorado, U.S.A.

A turf-banked lobe with a ground soil of post-Pinedale age overlies a buried paleosol which, although thin, appears to have been developed in situ. Organic matter from the Ab horizon of the paleosol yields a radiocarbon age of $\text{10,400 ± 400}\text{yrs}$ BP (Gak-3823) which corresponds with the waning stages of the Pinedale Glaciation in the Rocky Mountains. Abundant kaolinite and high amounts of silt and clay suggest that it may have developed over a long time interval.     

Proposals for a Sangay National Park in Ecuador

This paper describes the main features of the Sangay wilderness area in the Ecuadorian Andes. It is a region of great diversity with life zones ranging from tundra to subtropical rain forest. The conservation value of the area is assessed and the feasibility of protection determined. Boundaries based mainly on the limits of human interference are put forward with management proposals for its establishment as a National Park.     

Gas-liquid chromatographic/mass spectrometric screening method for T-2 toxin in milk

A method for the analysis of T-2 toxin in milk is presented. Ethyl acetate extracts of milk samples which had been spiked with T-2 toxin were purified by thin layer chromatography and derivatized with N,O-bis(trimethylsilyl)acetamide to produce the T-2 toxin trimethylsilyl ether (T-2 toxin-TMS). N,O-bis(trimethylsilyl-d9)acetamide was used to make T-2 toxin d9-trimethylsilyl ether (T-2 toxin-d9 TMS) which was added to the derivatized milk extract as an internal standard. Samples were analyzed by combined gas-liquid chromatography/mass spectrometry using either electron impact ionization or chemical ionization mass spectrometry. In electron impact ionization analyses, simultaneous monitoring of the T-2 toxin-TMS fragment ion at m/z 436 and the T-2 toxin-d9TMS fragment ion at m/z 445 gave a T-2 toxin-TMS detectability estimated at 6 microgram/kg. In chemical ionization analyses, the T-2 toxin-TMS fragment ion at m/z 377 and the T-2 toxin-d9TMS fragment ion at m/z 386 were simultaneously monitored to give a T-2 toxin-TMS detectability estimated at 3 microgram/kg. Average recovery was 85% at 200 microgram/kg and 65% at 20 microgram/kg.     

A comparison of methods of recovering oesophageal extrusa from grazing sheep

Extrusa samples were collected from two groups of oesophageally fistulated sheep grazing together. One group had foam plastic oesophageal plugs fitted below the fistula to assist in the complete collection of extrusa; the second group grazed without plugs. The extrusa were collected in four periods chosen to cover as wide a range of herbage conditions as possible varying from herbage with a high proportion of green leaves to predominantly dry material with a high proportion of dead leaves and stems. The plant components of the extrusa were determined and there was no evidence that stems or any other plant component preferentially by-pass the fistula in grazing sheep. It was concluded that it is not necessary to insert foam plastic plugs in the oesophagus of grazing sheep to obtain representative samples of ingested herbage.     

Conservation assessment of British limestone pavements based on floristic criteria

The total British area of limestone pavement is very small, yet this scarce resource is being destroyed to supply decorative stone. Its scientific interest is both geological and botanical. Exploiting an exhaustive survey, a weighted index was developed for assessing the conservation value of limestone pavements based on their deep grike floras. Using weighted indices, 27 sites in Cumbria, Gwynedd, Lancashire, North Yorkshire and Skye were identified as important for the conservation of limestone pavement floras.     

Identification of some analogs of the hallucinogen phencyclidine.

The drugs 1-[1-(2-thienyl)cyclohexyl]piperidine, 1-[1-2-thienyl)cyclohexyl]pyrrolidine, 1-(-phenylcyclohexyl) morpholine, and 1-(1-phenyleyclohexyl) pyrrolidine are identified by spectroscopic techniques. The ultraviolet and proton magnetic resonance spectra of analogs are similar, but mass and infrared spectra are distinctly different, and reference spectra are provided. Gas-liquid ant thin layer chromatographic systems for the analysis are discussed.