不同利用方式对贝加尔针茅草原土壤活性有机碳的影响

土壤活性有机碳能够准确反映土壤有效性,表征土壤质量变化,是探索可持续草地管理措施的关键指标之一。以内蒙古贝加尔针茅草原为研究对象,采用围封、放牧和刈割野外控制试验,探讨不同利用方式对土壤有机碳（SOC）和活性有机碳的影响,发现不同利用方式下土壤SOC含量表现为围封>刈割>放牧,其中围封区和刈割区土壤SOC含量显著大于放牧区,围封区与刈割区土壤SOC含量差异不显著,在土壤活性有机碳中土壤可溶性有机碳（DOC）含量表现为放牧>围封>刈割。土壤微生物量碳（MBC）和土壤易氧化有机碳（ROC）含量均表现为围封>刈割>放牧,围封区与刈割区土壤MBC和土壤ROC平均含量差异不显著,且均显著大于放牧区。土壤MBC、ROC和SOC之间呈极显著相关性（P<0.01）。土壤ROC和土壤MBC与土壤全氮和土壤全磷呈极显著相关性（P<0.01）。围封与刈割有利于土壤SOC、MBC、ROC的提升,放牧对土壤DOC有一定累积作用。围封和刈割增强了土壤SOC的稳定性,活性有机碳与土壤有机碳和土壤理化性质密切相关,能够敏感地反映土壤有机碳的变化。     

基于EDA技术的自动立木整枝机无线遥控发射系统

立木整枝是优质工业用材林定向抚育的重要环节,采用自动立木整枝机是实现高效、高质量和安全可靠整枝的有效途径。本文综合运用电子电路设计及优化技术、无线遥控及通信编解码技术,创造性地研制了基于CPLD的无线数字编码抗干扰自动立木整枝机无线遥控发射系统,通过优化CPLD内部逻辑电路有效消除了逻辑竞争冒险、降低了噪声干扰,确保了系统逻辑设计的正确性。该系统具有可靠、节能、抗干扰、工作效率高等优点。本研究成果及方法为推进其他林业机械的自动化与智能化进程提供了一种有效的新思路。     

The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation

The aim of this study was to examine the effect of varying intracellular reactive oxygen species (ROS) levels during oocyte in vitro maturation with enzymatic ROS production systems (xanthine + xanthine oxidase or xanthine + xanthine oxidase + catalase), scavenger systems (catalase or superoxide dismutase + catalase) or cysteine on porcine oocyte maturation. Oocyte ROS levels showed an increase when H₂O₂ or O₂?^‐ production systems were added to the culture medium (p < 0.05). On the other hand, the presence of ROS scavengers in the maturation medium did not modify oocyte ROS levels compared with the control after 48 h of maturation, but the addition of cysteine induced a decrease in oocyte ROS levels (p < 0.05). The ROS production systems used in this work did not modified the percentage of oocyte nuclear maturation, but increased the decondensation of sperm head (p < 0.05) and decreased the pronuclear formation (p < 0.05). In turn, the addition of O₂?^‐ and H₂O₂ scavenging systems during in vitro maturation did not modify the percentage of oocytes reaching metaphase II nor the oocytes with decondensed sperm head or pronuclei after fertilization. However, both parameters increased in the presence of cysteine (p < 0.05). The exogenous generation of O₂?^‐ and H₂O₂ during oocyte in vitro maturation would not affect nuclear maturation or later sperm penetration, but most of the spermatozoa cannot progress to form the pronuclei after fusion with the oocyte. The decrease in endogenous ROS levels by the addition of cysteine would improve pronuclear formation after sperm penetration.     

Determination of reference intervals for metabolic profile of Hanwoo cows at early,middle and late gestation periods

Background

Metabolic profile was initially designed as a presymptomatic diagnostic aid based on statistical analyses of blood metabolites to provide an early warning of certain types of metabolic disorder. However, there is little metabolic profile data available about Korean Hanwoo cows. Therefore, this study aimed to determine the reference intervals of metabolic profile for Korean Hanwoo cows.

Methods

Healthy animals (2,205) were selected and divided into early (day 1 to 95), middle (day 96 to 190) and late (day 191 to 285) period according to their gestating period. Metabolic profile including total protein (TP), albumin (Alb), urea (UREA), glucose (Glu), total cholesterol (T-Cho), long-chain fatty acid (LCFA), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), creatinine (Crea), calcium (Ca), inorganic phosphorous (iP) and magnesium (Mg) were analyzed using a TBA-40FR automatic biochemical analyzer. The data of Korean Hanwoo cows were then compared to those of the Japanese Wagyu cows.

Results

Most of the data of the Korean Hanwoo cows were relatively higher than those of Japanese Wagyu cows, with the exception of Glu and GGT. This may indicate that the nutritional level of feed for the Korean Hanwoo cows was higher than that of the Japanese Wagyu cows because of the different feeding system. In particular, relatively higher levels of UREA and LCFA were observed in the Korean Hanwoo cows, and this may also contribute to the low reproduction efficiency.

Conclusions

These findings may provide some theoretical basis for understanding the reproductive and feeding situation of Korean Hanwoo cows.     

Methane emissions and δ13C composition from beef steers consuming increasing proportions of sericea lespedeza hay on bermudagrass hay diets

An experiment was conducted to evaluate the effects of different proportions of ‘Au Grazer’ sericea lespedeza [SL, Lespedeza cuneata (Dum. Cours.) G. Don], a legume rich in condensed tannins (CT), on nutrient intake and digestibility, and to estimate methane (CH₄) emissions and ¹³C isotopic composition (δ¹³C_CH4) from beef steers consuming a forage-based diet. Twenty-five Angus-crossbred steers were distributed in a randomized complete block design (344 ± 48 kg initial BW), and randomly assigned to one of five treatments: 0SL, 25SL, 50SL, 75SL, and 100SL, diets containing 0%, 25%, 50%, 75%, and 100% of SL hay, respectively, mixed with ‘Tifton-85’ bermudagrass hay (Cynodon spp.). The study was carried out for two experimental periods of 21-d each. The statistical model included the fixed effect of treatment and random effects of block, experimental period, and their interaction. Apparent total tract digestibility of crude protein, neutral detergent fiber, and acid detergent fiber was linearly decreased (P < 0.001) by the inclusion of SL. No effects were observed for total CH₄ emissions per day, nor for CH₄ relative to organic matter intake or digestible organic matter with the inclusion of SL. However, emission of CH₄ in relation to intake of CT was affected by treatment (P < 0.001). A linear (P < 0.001) decrease and a quadratic effect (P < 0.001) were observed for δ¹³C of diets and gas, respectively, in which diets and enteric CH₄ with greater inclusion of SL were more depleted in ¹³C. Moreover, the difference in δ¹³C between diets and gas (Δδ¹³C) had a linear decrease (P = 0.001) with the inclusion of SL. The model developed to predict the C₃ proportions in the enteric CH₄ fitted to predicted values (P < 0.0001). Therefore, greater proportions of SL resulted in lesser CH₄ emission when CT intake was considered and the isotopic composition from enteric CH₄ was able to predict the contribution of SL in the emissions.     

油用亚麻主要品质和农艺性状的变异分析

为深入了解油用亚麻主要品质和农艺性状的遗传变异,以253份油用亚麻种质为研究对象,在3个环境下（内蒙古呼和浩特市、集宁市和锡林郭勒盟太仆寺旗）对其全生育天数、株高、工艺长度、单株果数、每果粒数、单株粒重、千粒重和分枝数8个农艺性状及棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸、粗脂肪含量6个品质性状进行鉴定。结果表明,8个农艺性状的变异系数为5.66%~42.65%;6个品质性状的变异系数为4.10%~30.14%。全生育天数在太仆寺旗表现最长,为112.51 d;千粒重和单株粒重在集宁地区表现最大,分别为5.94和0.55 g;果粒数和单株果数在呼和浩特地区表现最多,分别为5.65和16.90;粗脂肪和亚麻酸含量在太仆寺旗地区表现最大,分别为39.53%和53.45%;亚油酸和棕榈酸含量在集宁地区最大,分别为16.41%和5.09%;油酸和硬脂酸含量在呼和浩特地区表现最大,分别为24.03%和8.31%。聚类分析表明,253份油用亚麻种质被划分为4个类群,相同地理来源的油用亚麻种质被聚到1个类群,为油用亚麻种质资源的收集保存与繁殖提供了科学依据。     

人工林UHF频段电波传播场强预测模型

人工林无线电波传播场强模型是合理设计人工林环境无线电遥测遥控等通信电路的理论依据.该文在分析无线电波传播机理的基础上确定了人工林UHF频段传播损耗的三大组成部分及相应的计算方法,提出了人工林中某接收点处电场强度的预测模型.经过对北京西山人工侧柏林中不同参数的场强测试数据进行分析,校验了所建模型具有很高的精度.      

Gene interaction at HLA-DQ enhances autoantibody production in primary Sj?gren's syndrome

Primary Sj?gren's syndrome is an autoimmune disorder characterized by dryness of the mouth and eyes. The human leukocyte antigen (HLA) locus DQ is related to the primary Sj?gren's syndrome autoantibodies that bind the RNA proteins Ro/SSA and La/SSB. Both DQ1 and DQ2 alleles are associated with high concentrations of these autoantibodies. An analysis of all possible combinations at DQ has shown that the entire effect was due to heterozygotes expressing the DQ1 and DQ2 alleles. These data suggest that gene interaction between DQ1 and DQ2 (or alleles at associated loci), possibly from gene complementation of trans-associated surface molecules, influences the autoimmune response in primary Sj?gren's syndrome.