Marennine,Promising Blue Pigments from a Widespread Haslea Diatom Species Complex

In diatoms, the main photosynthetic pigments are chlorophylls a and c, fucoxanthin, diadinoxanthin and diatoxanthin. The marine pennate diatom Haslea ostrearia has long been known for producing, in addition to these generic pigments, a water-soluble blue pigment, marennine. This pigment, responsible for the greening of oysters in western France, presents different biological activities: allelopathic, antioxidant, antibacterial, antiviral, and growth-inhibiting. A method to extract and purify marennine has been developed, but its chemical structure could hitherto not be resolved. For decades, H. ostrearia was the only organism known to produce marennine, and can be found worldwide. Our knowledge about H. ostrearia-like diatom biodiversity has recently been extended with the discovery of several new species of blue diatoms, the recently described H. karadagensis, H. silbo sp. inedit. and H. provincialis sp. inedit. These blue diatoms produce different marennine-like pigments, which belong to the same chemical family and present similar biological activities. Aside from being a potential source of natural blue pigments, H. ostrearia-like diatoms thus present a commercial potential for aquaculture, cosmetics, food and health industries.     

Effects of long-term contrasting lime and phosphorus applications on barley grain yield,root growth and abundance of mycorrhiza

Lime and phosphorus (P) applications are common agricultural management practices. Our aim was to quantify the effects of long-term application practices on root growth and abundance of arbuscular mycorrhizal fungi (AMF) under field conditions. We assessed the effects of lime and P fertilizer applications on barley yield, root growth and AMF abundance in 2016. Treatments were no, low, medium and high liming rate corresponding to application of 0, 4, 8 and 12 Mg lime ha⁻¹ every 5–9 years since 1942 combined with no or yearly application of 15.6 kg P ha⁻¹ since 1944. At harvest, grain yield, root intensity (core-break) and AMF abundance at different soil depths were estimated. Root development was monitored during early growth with minirhizotrons in treatments receiving low, medium and high liming rates and P fertilization. A quadratic model relating grain yield to liming rate estimated yields to peak at 6.4 Mg lime ha⁻¹ with yields of 4.2 and 3.2 Mg grain ha⁻¹ with and without P fertilization, respectively. Low and medium liming rates resulted in greater AMF abundance, especially in the no P treatments. During early growth in P-fertilized treatments, 77% and 65% more roots developed in the soil profile when treated with medium and high liming rate, respectively, compared to low liming rate. We conclude that long-term application of lime in soils receiving yearly P fertilization improved conditions for root growth in soil layers below 30 cm, but at the high liming rate, this did not translate into higher yield.     

Ash and biochar amendment of coarse sandy soil for growing crops under drought conditions

Appropriate soil amendments may increase plant available water and crop yields on coarse sandy soils under drought conditions. In this study, we applied straw ash or straw biochar from gasification to a Danish coarse sandy subsoil to assess the effects on soil water retention, evapotranspiration and crop yields. Spring barley (2016, 2017) and winter wheat (2018) were grown over three years in columns containing 25cm of organic matter-rich topsoil, 80 cm of amended coarse sandy soil (1.5%, 3%, 6% wt. ash or 1% wt. biochar or control soil) and 45 cm of un-amended subsoil. Precipitation, evaporative demands and soil moisture were recorded across the growth seasons, with 2018 having severe drought conditions. This year evapotranspiration levels increased with increasing ash and biochar content (by 54% and 33% for the 6% ash- and 1% biochar-amended soils, respectively), and plant dry matter increased by 18% in both the 1% biochar- and 6% ash-treated soils compared to the untreated control. A linear relationship was established between in situ field capacity and ash dosage (R² = .96), showing an increase of 2.2% per percentage (wt.) of ash added, while the 1% biochar treatment increased the capacity by 3.5%, indicating a higher efficiency than for ash. However, we did not find significant positive effects on grain yields. The results show that ash and biochar have the potential to significantly increase soil water retention, evapotranspiration and total dry matter yield in drought conditions, but that this may not correspond to an increase in grain yield.     

Growth and thinning effects during a rotation period of hybrid aspen in southern Sweden

Hybrid aspen is an interesting tree species for wood production in northern Europe. In this study we examined growth dynamics over the whole rotation period. Height and diameter development, as well as annual growth of stem volume and stem biomass, were repeatedly recorded in 14 planted and 2 root sucker stands, aged up to 26 years, in southern Sweden. A main aim was to study the productivity level for hybrid aspen forestry with an expected rotation period of about 25 years. The study verified earlier prognoses, showing a mean annual increment (MAI) of 19.5 m³ of stem wood ha^?1 yr^?1 after 25 years. This corresponds to a dry weight of stem biomass of 7.2 tons dry matter (DM) ha^?1 yr^?1. Maximum MAI was still not reached after 25 years, although the growth curve was flattening out. If branch biomass is included, MAI is estimated to about 9 tons DM ha^?1 yr^?1 and further improvements in growth is expected by using the best genotypically selected clones available. Results from three different thinning regimens showed that thinning intensity provided significantly larger diameter growth, while no significant yield effects were seen among the thinning regimens.     

Effects of harvesting date and storage on the amounts of polyacetylenes in carrots, Daucus carota

The amounts of three main polyacetylenes in carrots; falcarinol, falcarindiol, and falcarindiol-3-acetate, were determined by HPLC, during three seasons, in carrots harvested several times per season and at different locations in Sweden. The amounts of falcarindiol first decreased from a relatively high level and then increased later in the harvest season. The amounts of falcarindiol-3-acetate showed similar variations, whereas the amounts of falcarinol did not exhibit any significant variation during the harvest season. During storage the amount of polyacetylenes leveled off, increasing in samples initially low and decreasing in samples initially high in polyacetylenes. The amounts of all polyacetylenes varied significantly due to external factors and between stored and fresh samples. This variation opens up possibilities to achieve a chemical composition of polyacetylenes at harvest that minimizes the risk of bitter off-taste and maximizes the positive health effects reported in connection with polyacetylenes in carrots.     

A comparison of linear and exponential regression for estimating diffusive CH4 fluxes by closed-chambers in peatlands

The closed-chamber method is the most common approach to determine CH₄ fluxes in peatlands. The concentration change in the chamber is monitored over time, and the flux is usually calculated by the slope of a linear regression function. Theoretically, the gas exchange cannot be constant over time but has to decrease, when the concentration gradient between chamber headspace and soil air decreases. In this study, we test whether we can detect this non-linearity in the concentration change during the chamber closure with six air samples. We expect generally a low concentration gradient on dry sites (hummocks) and thus the occurrence of exponential concentration changes in the chamber due to a quick equilibrium of gas concentrations between peat and chamber headspace. On wet (flarks) and sedge-covered sites (lawns), we expect a high gradient and near-linear concentration changes in the chamber. To evaluate these model assumptions, we calculate both linear and exponential regressions for a test data set (n = 597) from a Finnish mire. We use the Akaike Information Criterion with small sample second order bias correction to select the best-fitted model. 13.6%, 19.2% and 9.8% of measurements on hummocks, lawns and flarks, respectively, were best fitted with an exponential regression model. A flux estimation derived from the slope of the exponential function at the beginning of the chamber closure can be significantly higher than using the slope of the linear regression function. Non-linear concentration-over-time curves occurred mostly during periods of changing water table. This could be due to either natural processes or chamber artefacts, e.g. initial pressure fluctuations during chamber deployment. To be able to exclude either natural processes or artefacts as cause of non-linearity, further information, e.g. CH₄ concentration profile measurements in the peat, would be needed. If this is not available, the range of uncertainty can be substantial. We suggest to use the range between the slopes of the exponential regression at the beginning and at the end of the closure time as an estimate of the overall uncertainty.     

Effects of Plant and Soil Factors on Growth of Young Apple and Blackcurrant Plants

Abstract

Shoot growth as affected by plant size at planting, peat application in the planting hole, pruning, drip-irrigation and, for apple, specific apple replant disease (SARD) was investigated in apple (cv. Elstar) and blackcurrant (cvs Ben Lomond and Ben Nevis), using a multifactorial design. In the first year after planting, peat application and drip-irrigation enhanced shoot growth in both species, with a further interactional increase when both treatments were applied. The extension growth of apple, in contrast to blackcurrant, was positively related to plant size, and the strength of the relationship was distinctly improved when growth conditions were good. Pruning had only small effects on total shoot growth, with a trend towards an increase when growth conditions were poor.     

Seasonal modulation of puberty and the postpartum anestrus in cattle: A review

While cattle are not usually considered seasonal breeders, both age at puberty and length of the postpartum anestrus can be modified by season. Specifically, autumn and winter environments during the first six months of life hasten puberty, while the same conditions after six months delay puberty. Cows calving in the winter have delayed resumption of estrous cycles compared with summer-calving cows. Effects of season interact in a complex manner with other environmental factors affecting reproduction, including breed, nutrition, level of milk production and suckling. While some seasonal variation is undoubtedly caused by variations in management, effects of season on puberty and the postpartum anestrus can be mimicked by altering day length, suggesting that photoperiod is one of the environmental stimuli responsible for seasonal effects. The physiological mechanisms by which season alters reproduction in cattle are unclear, but may include changes in folliculogenesis, secretion of luteinizing hormone and growth rate. Based on studies of prolactin secretion, photoperiodic signals are probably transmitted via the retina and pineal. It is suggested that the existence of seasonal effects on reproduction may create the propensity for spring and summer calving.     

The occurrence of summer mastitis in Jutland (Denmark) in relation to meteorological factors

Data for incidence of summer mastitis in 14 veterinary practices in Jutland (Denmark) were related to meteorological factors using statistical methods. A correlation and regression analysis showed that summer mastitis occurred earlier in years with high temperature and precipitation in early summer. The annual number of cases markedly increased over the years 1953 to 1980. This increase was described by applying a linear increase with with year to a logarithmic transformation of the annual incidence.Analysis of 10-day incidences from the veterinary practices and daily incidence from a test site at St. Vildmose (Jutland) showed that incidence of summer mastitis could be related to temperature and wind velocity within the previous 10 days. High temperature and low wind velocity in this period increase the incidence. This indicates that the incubation period of summer mastitis is at maximum about 10 days. The activity of the fly (Hydrotaea irritans Fall), which acts as mechanical vector for the bacteria is influenced by temperature and wind velocity. This may explain the variation in incidence of summer mastitis during the season and from year to year.