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991.
文中介绍了应用微机研究拖拉机耗油的方法。该方法以理论分析为基础,结合经验关系式建立了拖拉机油耗的微机模拟模型。模型输出与田间实验测得的数据进行了比较。比较结果表明,除了发动机转速特别低的情况下,计算油耗与实际测得的油耗基本一致。本文还利用该模拟模型分析了影响拖拉机油耗的因素。正确有效的操作可提高燃油利用率。 相似文献
992.
Xiao H Jiang N Schaffner E Stockinger EJ van der Knaap E 《Science (New York, N.Y.)》2008,319(5869):1527-1530
Edible fruits, such as that of the tomato plant and other vegetable crops, are markedly diverse in shape and size. SUN, one of the major genes controlling the elongated fruit shape of tomato, was positionally cloned and found to encode a member of the IQ67 domain-containing family. We show that the locus arose as a result of an unusual 24.7-kilobase gene duplication event mediated by the long terminal repeat retrotransposon Rider. This event resulted in a new genomic context that increased SUN expression relative to that of the ancestral copy, culminating in an elongated fruit shape. Our discovery demonstrates that retrotransposons may be a major driving force in genome evolution and gene duplication, resulting in phenotypic change in plants. 相似文献
993.
The fusion pore of regulated exocytosis is a channel that connects and spans the vesicle and plasma membranes. The molecular composition of this important intermediate structure of exocytosis is unknown. Here, we found that mutations of some residues within the transmembrane segment of syntaxin (Syx), a plasma membrane protein essential for exocytosis, altered neurotransmitter flux through fusion pores and altered pore conductance. The residues that influenced fusion-pore flux lay along one face of an alpha-helical model. Thus, the fusion pore is formed at least in part by a circular arrangement of 5 to 8 Syx transmembrane segments in the plasma membrane. 相似文献
994.
Treusch S Hamamichi S Goodman JL Matlack KE Chung CY Baru V Shulman JM Parrado A Bevis BJ Valastyan JS Han H Lindhagen-Persson M Reiman EM Evans DA Bennett DA Olofsson A DeJager PL Tanzi RE Caldwell KA Caldwell GA Lindquist S 《Science (New York, N.Y.)》2011,334(6060):1241-1245
Aβ (beta-amyloid peptide) is an important contributor to Alzheimer's disease (AD). We modeled Aβ toxicity in yeast by directing the peptide to the secretory pathway. A genome-wide screen for toxicity modifiers identified the yeast homolog of phosphatidylinositol binding clathrin assembly protein (PICALM) and other endocytic factors connected to AD whose relationship to Aβ was previously unknown. The factors identified in yeast modified Aβ toxicity in glutamatergic neurons of Caenorhabditis elegans and in primary rat cortical neurons. In yeast, Aβ impaired the endocytic trafficking of a plasma membrane receptor, which was ameliorated by endocytic pathway factors identified in the yeast screen. Thus, links between Aβ, endocytosis, and human AD risk factors can be ascertained with yeast as a model system. 相似文献
995.
本文以文献及作者在果树上的研究为基础,对植物离子吸收动力学的基本概念、研究方法、影响动力学参数的主要因素和在果树营养研究中的应用,以及离子吸收动力学的意义和发展前景进行了述评。 相似文献
996.
作者对136峰双峰驼的血液蛋白多态性作了分析,通过聚丙烯酰胺凝胶电泳测定血红蛋白(Hb)、血清白蛋白(Alb)、后白蛋白(Pa)和运转蛋白(Tf)得出的结果表明:双峰驼的Hb,Alb,Pa和Tf只有一种基因型,而且全部纯合,不表现多态性现象。同时还发现6个月以下的幼驼没有驼羔血红蛋白存在。 相似文献
997.
998.
Huang D Luo L Jiang C Han J Wang J Zhang T Jiang J Zhou Z Chen H 《Journal of agricultural and food chemistry》2011,59(6):2148-2156
Plant research and natural product detection are of sustainable interests. Benefited by direct detection with no sample preparation, sinapine, a bioactive chemical usually found in various seeds of Brassica plants, has been unambiguously detected in radish taproot (Raphanus sativus) tissue using a liquid-assisted surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS). A methanol aqueous solution (1:1) was nebulized by a nitrogen sheath gas toward the corona discharge, resulting in charged ambient small droplets, which affected the radish tissue for desorption/ionization of analytes on the tissue surface. Thus, sinapine was directly detected and identified by tandem DAPCI-MS experiments without sample pretreatment. The typical relative standard deviation (RSD) of this method for sinapine detection was 5-8% for six measurements (S/N=3). The dynamic response range was 10(-12)-10(-7) g/cm2 for sinapine on the radish skin surface. The discovery of sinapine in radish taproot was validated by using HPLC-UV methods. The data demonstrated that DAPCI assisted by solvent enhanced the overall efficiency of the desorption/ionization process, enabling sensitive detection of bioactive compounds in plant tissue. 相似文献
999.
1000.