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21.
ZF Ren ZP Huang JW Xu JH Wang P Bush MP Siegal PN Provencio 《Science (New York, N.Y.)》1998,282(5391):1105-1107
Free-standing aligned carbon nanotubes have previously been grown above 700 degreesC on mesoporous silica embedded with iron nanoparticles. Here, carbon nanotubes aligned over areas up to several square centimeters were grown on nickel-coated glass below 666 degreesC by plasma-enhanced hot filament chemical vapor deposition. Acetylene gas was used as the carbon source and ammonia gas was used as a catalyst and dilution gas. Nanotubes with controllable diameters from 20 to 400 nanometers and lengths from 0. 1 to 50 micrometers were obtained. Using this method, large panels of aligned carbon nanotubes can be made under conditions that are suitable for device fabrication. 相似文献
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JI Yun HJ Park MH Park MS Kim JH Choi E Lee SP Gong JM Lim ST Lee 《Reproduction in domestic animals》2014,49(5):705-710
Recently, isolation and in vitro culture of putative spermatogonial stem cells (SSCs) in the domestic cat have been conducted. However, the cellular niche conditions that facilitate the establishment and long‐term maintenance of feline SSCs (FSSCs) have not been described. Therefore, we investigated the type of feeder cells used to stimulate colony formation and growth of FSSCs among the various factors in the FSSC niche. Spermatogonial stem cells isolated from feline testes were cultured on mitotically inactivated testicular stromal cells (TSCs) derived from cats, dogs and mice, and mouse embryonic fibroblasts (MEFs). The formation and growth of colonies derived from SSCs cultured on each type of feeder cell were identified at passage 0, and the morphology, alkaline phosphatase (AP) activity and expression of SSC‐specific genes in surviving colonies were investigated at passage 4. Among these diverse feeder cells, TSCs from cat showed the greatest colony formation, growth and maintenance of FSSCs, and SSC colonies cultured by passage 4 showed a typical dome‐shaped morphology, AP activity and expression of SSC‐specific genes (NANOG, OCT4, SOX2 and CD9). Accordingly, these results demonstrate that feline TSCs could be used as feeder cells to support the establishment and maintenance of SSCs from domestic cats. 相似文献
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DJ STEWART JA VAUGHAN TC ELLEMAN† PA HOYNE† KJ BURNS JH DUFTY 《Australian veterinary journal》1991,68(2):50-53
The relationship between the serological classification system for serogroup B and for serogroup H of Bacteroides nodosus and cross-protection between subgroups within these serogroups was examined. Protection against ovine footrot following vaccination was achieved against other subgroup strains provided sufficient cross-reactive antibody was induced by shared pilus antigens. Within serogroup B, better cross-protection against one subgroup was obtained with a pili vaccine than a whole cell vaccine which correlated with higher pilus antibody titres induced by the former. For serogroup H, a lack of cross-protection and serological reactivity between subgroups was demonstrated, which indicates that the prototype strain of subgroup H2 should be designated a new serogroup. 相似文献
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The microscopic particle motions from the crystal to the disordered state of a dusty plasma with micrometer-sized silicon dioxide particle suspensions in a radio-frequency glow discharge system were studied through an optical microimaging system. Small-amplitude random motion around the lattice sites of the crystal state, relative domain motion with varying boundaries, cooperative hopping in the liquid state, and highly disordered motion with increasing radio-frequency power were observed. Chaotic states with different spatial scales under the coherent and stochastic coupling between dust particles and self-organized background plasma fluctuations were also demonstrated. 相似文献
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