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41.
The aims of this study were to determine the concentrations of the progesterone, oestradiol‐17‐β, vitamin A, C and β‐carotene in plasma and cyst fluid and to relate these values with cystic diameter and membrane thickness of Holstein cattle with ovarian luteal cyst. 1650 Holstein cows were examined for the presence of the ovarian cyst and luteal and follicular cystic ovaries were obtained following slaughtering in personal slaughterhouse in Konya‐Turkey. 15 Luteal and 15 follicular cystic ovaries were distinguished by rectal palpation and by post mortem ultrasonographic examination. Plasma and cyst fluid, hormone and vitamin analyses were carried out by EIA method and spectrophotometric measurement respectively. Although there was no relationship between β‐carotene and vitamin A in plasma and cyst fluid of both cyst type and hormone concentrations, the vitamin C concentration of cyst fluid was found significantly higher in luteal cyst than in follicular cyst. Moreover, there is a positive correlation among values of the vitamin C concentrations of cyst fluid and cystic membrane thickness, plasma and the cyst fluid progesterone concentrations, but there is a negative correlation among the vitamin C concentrations of cystic fluid and oestradiol 17β levels of plasma and cyst fluid. In conclusion, vitamin C concentration of cyst fluid supported ultrasonographic and endocrinologic findings. Also, it can be postulated that vitamin C is probably effective on progesterone synthesis in the luteal tissue of cyst. 相似文献
42.
Ayşe Kılıc Hakan Kalender Hatice Eroksuz Adile Muz Bülent Tasdemir 《Tropical animal health and production》2013,45(7):1525-1531
This study used cultures, polymerase chain reaction (PCR), and immunoperoxidase to examine samples from 216 lungs from sheep and lambs with macroscopic pneumonia lesions for the presence of Mycoplasma species. DNA was extracted from lung tissue samples and broth cultures with the help of a DNA extraction kit and replicated using genus-specific and species-specific primers for mycoplasma. The lung samples were examined by the immunoperoxidase method using hyperimmune Mycoplasma ovipneumoniae serum. The randomly amplified polymorphic DNA (RAPD) test was used for the molecular typing of M. ovipneumoniae isolates. Mycoplasma was isolated in the cultures of 80 (37.03 %) of a total of 216 lung samples. Genus-specific mycoplasma DNA was identified by PCR in 96 (44.44 %) samples in broth cultures and 36 (16.66 %) directly in the lung tissue. Of these 96 cases in which genus-specific identification was made, 57 (59.37 %) were positive for reaction with species-specific primers for M. ovipneumoniae and 31 (32.29 %) for Mycoplasma arginini. The DNA of neither of the latter two species could be identified in the remaining eight samples (8.33 %) where mycoplasma had been identified. As for the immunoperoxidase method, it identified M. ovipneumoniae in 61 of 216 lung samples (28 %). Positive staining was concentrated in the bronchial epithelium cell cytoplasm and cell surface. RAPD analysis resulted in 15 different profiles. Our results suggest that PCR methods could be successfully used in the diagnosis of mycoplasma infections as an alternative to culture method and identifying this agent at the species level. 相似文献
43.
Dabak M Dabak DO Karapinar T Bulut H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):125-128
The aim of the present study was to determine the vitamin D status in cattle with malignant catarrhal fever (MCF). Twelve cattle diagnosed as MCF and 6 healthy cattle (controls) were used in the study. Serum 1,25-dihydroxyvitamin D(3) (1,25-D), 25-hydroxyvitamin D(3) (25-D), calcium, phosphorus and parathyroid hormone (PTH) levels were determined as 96.83 pg/ml, 30.0 ng/ml, 2.19 mmol/l, 1.57 mmol/l and 15.21 pg/ml in MCF group and 42.33 pg/ml, 37.0 ng/ml, 2.43 mmol/l, 1.96 mmol/l and 36.08 pg/ml in controls, respectively. Although serum 1,25-D level in the MCF group was increased (P<0.01), serum calcium (P<0.01) and PTH (P<0.05) levels were decreased compared to the controls. The results suggest that there might be an interaction between vitamin D status and MCF. 相似文献
44.
Hakan Özkan George Willcox Andreas Graner Francesco Salamini Benjamin Kilian 《Genetic Resources and Crop Evolution》2011,58(1):11-53
The transition from hunting and gathering to agriculture had revolutionary consequences for the development of human societies.
Crops such as wheat, barley, lentil, pea and chickpea played a crucial role in the establishment of complex civilizations
in south west Asia. Wild emmer wheat (Triticum dicoccoides) was one of the first cereals to be domesticated in the Fertile Crescent between c. 12,000 and c. 10,000 years ago. This
step provided the key for subsequent bread wheat evolution. Wild emmer is found today in the western Fertile Crescent in Jordan,
Syria and Israel, the central part of southeastern Turkey and mountain areas in eastern Iraq and western Iran. In this review,
we summarize issues concerning geography and domestication of wild emmer wheat based on published molecular and archaeobotanical
data and on our recent findings. We suggest that modern domestic tetraploid wheats derived from wild emmer lines from southeast
Turkey. However, our understanding of emmer domestication is not complete. The “dispersed-specific” domestication model proposed
for einkorn might well be appropriate also for emmer. 相似文献
45.
Ali Osman Demir Abdurrahim Tanju Göksoy Hakan Büyükcangaz Zeki Metin Turan Eyüp Selim Köksal 《Irrigation Science》2006,24(4):279-289
The response of sunflower (Helianthus annuus L.) to 14 irrigation treatments in a sub-humid environment (Bursa, Turkey) was studied in the field for two seasons. A rainfed (non-irrigated) treatment as the control and 13 irrigation treatments with full and 12 different deficit irrigations were applied to the hybrid Sanbro (Novartis Seed Company) planted on clay soil, at three critical development stages: heading (H), flowering (F) and milk ripening (M). The yield increased with irrigation water amount, and the highest seed yield (3.95 t ha−1) and oil yield (1.78 t ha−1) were obtained from the HFM treatment (full irrigation at three stages); 82.9 and 85.4% increases, respectively, compared to the control. Evapotranspiration (ET) increased with increased amounts of irrigation water supplied. The highest seasonal ET (average of 652 mm) was estimated at the HFM treatment. Additionally, yield response factor (k
y) was separately calculated for each, two and total growth stages, and k
y was found to be 0.8382, 0.9159 (the highest value) and 0.7708 (the lowest value) for the total growing season, heading, and flowering-milk ripening combination stages, respectively. It is concluded that HFM irrigation is the best choice for maximum yield under the local conditions, but these irrigation schemes must be re-considered in areas where water resources are more limited. In the case of more restricted irrigation, the limitation of irrigation water at the flowering period should be avoided; as the highest water use efficiency (WUE) (7.80 kg ha−1 mm−1) and irrigation water use efficiency (IWUE) (10.19 kg ha−1 mm−1) were obtained from the F treatment. 相似文献
46.
Of the goat population in Turkey, 98% of the goat population consists of hair goats. They are the prime breeds of goats and they can be bred in the Mediterranean, Aegean and Southeast regions of Turkey. Hair goats are the main income for their breeders. The goats are typically raised in scarce conditions, thus giving rise to greater yield of meat. In spite of the fact that the studies are limited to hair goats bred by the public, in 2005, Republic of Turkey Ministry of Food, Agriculture and Livestock (TAGEM) initiated a countywide reclamation project aiming to identify the regional differences and characteristics of the hair goats. This paper focuses on the yearly quantity of hair goats' produce, particularly observing the proportions of goat milk, meat and other animal produce, and to highlight the importance of the hair goats. Recommendations are made from these findings. 相似文献
47.
Hakan Çelik Murat Ali Turan Barış Bülent Aşık Ali Vahap Katkat 《Communications in Soil Science and Plant Analysis》2017,48(21):2573-2581
For the comparison of the digestion and spectroscopic determination methods used to measure boron (B) concentrations in maize shoots, increasing doses of boron (0, 2.5, 5, and 10 mg kg?1 B) were applied to soil and maize plants (Zea mays L.) cultivar “Euralis Es Armandi” (FAO 640). The plants were grown under greenhouse conditions. Dried and ground maize shoots were digested according to two digestion procedures of microwave and dry ashing. Boron concentrations were determined using spectrophotometer and inductively coupled plasma-optical emission spectrometry (ICP-OES). The concentrations of boron influenced statistically (p < 0.01) due to the B applications and analysis methods. Spectrophotometric measurement of B amounts of the samples prepared with microwave digestion method was found irrelevant with other indicated methods. However, the ICP-OES measurements were found compatible with the dry ashing also with microwave digestion and suggested as a suitable method to determine boron contents of the maize shoots. 相似文献
48.
ABSTRACTEvaluation of pollen viability and its germination capacity are essential criteria for pollinator’s characterization. Hormones have been used to increase yield and quality of pomegranates without any reference to effects on pollen viability or germination. With this point of view, effects of field-applied 22S, 23S-homobrassinolide (HBR) after bud break at concentrations of 0.001, 0.01, and 0.1 mg L?1 were evaluated on pollen quality of Mayho?-8 pomegranate cultivar. After collection of the flowers of both sexual morphs (bisexual and functionally male), colorimetric test of 2,3,5-triphenyl tetrazolium chloride (1%) were used to estimate pollen viability. Later, pollen germination was determined in vitro on a medium containing 1% agar and 20% sucrose. Results showed that pollen viability was under the influence of both the types of the flower and the concentration of the HBR. The highest pollen viability (96%) was obtained from both functional male and bisexual flowers treated with 0.1 mg L?1 HBR. Germination ratio, on the other hand, was only affected by the hormone concentrations, and it increased significantly as the concentration was higher. 相似文献
49.
S Haliloglu N Baspinar B Serpek H Erdem & Z Bulut 《Reproduction in domestic animals》2002,37(2):96-99
This study was carried out to examine the relationship between the corpus luteum (CL) weight, CL and follicle diameters and progesterone, β‐carotene and vitamin A levels in reproductive organs of cattle obtained from the slaughterhouse. The β‐carotene and vitamin A levels were determined in plasma, CL and follicular fluid (FF) using a spectrophotometric method at different stages of the oestrous cycle (n=40) and at 3–6 months of pregnancy (n=10). The diameters of the CL and follicle were measured using ultrasonography. Plasma progesterone concentrations were determined by an enzyme immunoassay method. The vitamin A levels of the plasma, CL and FF were not related to each other. The highest plasma vitamin A levels were observed in the proestrus and oestrus, at which periods follicular activity dominates. The vitamin A levels in the CL and FF were negatively related to the weight and diameter of the CL and the diameter of follicle, respectively. In contrast to vitamin A, β‐carotene concentrations of plasma, CL and FF were significantly correlated with each other. The highest β‐carotene levels in the plasma, CL and FF were found during pregnancy when there is maximal luteal function, and the β‐carotene level of the CL was significantly correlated with the weight and diameter of CL. Furthermore, the intrafollicular β‐carotene level was negatively correlated with the follicle diameter. There was a positive correlation between plasma progesterone level and the weight and diameter of the CL, but a negative correlation between plasma progesterone level and follicle diameter. Moreover, plasma, FF and CL β‐carotene levels were positively correlated with plasma progesterone levels. This study revealed that β‐carotene levels in the plasma, CL and FF were influenced by the stage of the oestrous cycle or the pregnancy and were related to bovine luteal function without depending on vitamin A. 相似文献
50.
Wilson FH Hariri A Farhi A Zhao H Petersen KF Toka HR Nelson-Williams C Raja KM Kashgarian M Shulman GI Scheinman SJ Lifton RP 《Science (New York, N.Y.)》2004,306(5699):1190-1194
Hypertension and dyslipidemia are risk factors for atherosclerosis and occur together more often than expected by chance. Although this clustering suggests shared causation, unifying factors remain unknown. We describe a large kindred with a syndrome including hypertension, hypercholesterolemia, and hypomagnesemia. Each phenotype is transmitted on the maternal lineage with a pattern indicating mitochondrial inheritance. Analysis of the mitochondrial genome of the maternal lineage identified a homoplasmic mutation substituting cytidine for uridine immediately 5' to the mitochondrial transfer RNA(Ile) anticodon. Uridine at this position is nearly invariate among transfer RNAs because of its role in stabilizing the anticodon loop. Given the known loss of mitochondrial function with aging, these findings may have implications for the common clustering of these metabolic disorders. 相似文献