宿主域扩大的重组救活昆虫杆状病毒表达载体的构建及外源基因的表达

通过克隆的家蚕核多角体病毒解旋酶基因DNA与重组救活可线性化的苜蓿尺蠖核多角体病毒基因工程载体病毒 (BacPAK6 )DNA在昆虫细胞中发生重组 ,经累代筛选获得了既可感染家蚕又可感染秋粘虫细胞Sf 2 1的宿主域扩大的昆虫杆状病毒表达载体 (HyBacPAK6 )。HyBacPAK6DNA经Bsu36Ⅰ酶切后与含植酸酶基因的转移载体pVL1393 phy在家蚕细胞中重组后 ,通过蓝白斑筛选发现重组率可达 90 %以上。然而以杂交病毒为载体的外源基因表达量仅为以BmNPV为载体病毒的表达量的 2 0 %左右。分析认为HyBacPAK6可用于表达一些对蚕体有害的外源基因。     

有机铬的合成及其产品质量评价

三价铬离子是构成葡萄糖耐量因子的重要组成部分,能够增强胰岛素的作用,是人和动物不可缺少的微量元素之一。有机铬化合物在生物机体中具有多种极其重要的活性功能,如果人体缺铬,将导致糖尿病和其它相关疾病;动物缺铬,则会导致对应激敏感、免疫功能受抑制、繁殖功能下降以及胴体品质下降等。本文综述了有机铬化合物的生物功能及其合成方法,介绍了产品的检测及质量评价方法,并对其发展前景做了一定的展望。     

紫花苜蓿多叶型与三叶型品种同工酶的比较研究

采用聚丙烯酰胺凝胶电泳技术,对国外2种多叶型苜蓿及国内外5种三叶型苜蓿幼苗的5种同工酶进行了测定,将酶谱数量化,应用SPSS统计软件求出各品种间的遗传一致度及欧氏遗传距离,并进行了聚类分析.结果表明:1)测定的5种同工酶中过氧化物酶(PER)、酯酶(EST)、淀粉酶(AMY)具有多态性,综合此3种酶酶谱可以鉴定供试的7个品种;2)2个多叶型品种具有一共同特征酶带PER-4,国内品种的过氧化物酶活性明显高于国外品种;3)品种间的遗传一致度为0.826～0.952,欧氏遗传距离为0.500～1.118,多叶型品种间遗传一致度为0.904,欧氏遗传距离为0.500;4)等级聚类分析结果为,以欧氏遗传距离0.400为分界,供试品种分为3类,2个多叶型品种为一类,其余的5种三叶型品种分为另两类.     

陕西省动物研究所馆藏标本现状及分析

陕西省动物研究所现有馆藏标本5万余份,是秦岭地区野生动物保护及研究的重要参考资料。为加强标本管理,提高标本使用效率,我们在2008年4月至2009年6月对所内馆藏标本进行了系统分类整理,分析总结了馆藏标本的现实状况并就存在的问题进行了探讨,为标本数字化共享平台建设奠定了基础,并为今后科研院校在动物学研究领域的交流与合作奠定基础。     

猪源表皮葡萄球菌的分离与鉴定

葡萄球菌广泛分布于空气、饲料、饮水、地面及物体表面,在人及畜禽的皮肤、粘膜、肠道、呼吸道及乳腺中也存在,大部分无致病性[1].但使机体抵抗力下降的诸因素,特别是皮肤和粘膜受损时,可导致葡萄球菌病的发生.2006年9月,在河北昌黎地区某养猪场,从外地购买的100头仔猪陆续发生以背部、四肢渗出性皮炎为主要症状,并伴有体温升高、眼结膜潮红等症状的疾病.作者从该养猪场病猪皮肤溃疡浆液中分离出2株表皮葡萄球菌,并对分离菌的主要生物学特性进行了初步研究,旨在为临床上防治本病提供参考.     

奶酪风味的研究进展

综述了奶酪风味的研究进展,对奶酪的风味物质以及风味物质的提取与分析方法作了重点概述.     

In vitro effects and in vivo efficacy of a novel cyclooxygenase-2 inhibitor in dogs with experimentally induced synovitis

OBJECTIVE: To determine cyclooxygenase-2 (COX-2) selectivity, pharmacokinetic properties, and in vivo efficacy of ML-1,785,713 in dogs. ANIMALS: 21 healthy male and female mixed-breed dogs and 24 healthy male Beagles. PROCEDURE: Selectivity of ML-1,785,713 for inhibiting COX-2 was determined by comparing the potency for inhibiting cyclooxygenase-1 (COX-1) with that of COX-2 in canine blood. Pharmacokinetic properties were determined after i.v. (2 mg/kg) and oral (8 mg/kg) administration in female mixed-breed dogs. In vivo efficacy was evaluated in male mixed-breed dogs with urate crystal-induced synovitis. Prophylactic efficacy was evaluated by administering ML-1,785,713 two hours before induction of synovitis whereas therapeutic efficacy was determined by administering ML-1,785,713 one hour after induction of synovitis. RESULTS: Blood concentrations that resulted in 50% inhibition of COX-1 and COX-2 activity in vitro were 119.1 microM and 0.31 microM, respectively, and selectivity ratio for inhibiting COX-2 relative to COX-1 was 384. ML-1,785,713 had high oral bioavailability (101%), low systemic clearance (77 mL/min/kg), and an elimination half-life of 5.9 hours. ML-1,785,713 was efficacious when administered prophylactically and therapeutically to dogs with urate crystal-induced synovitis. CONCLUSIONS AND CLINICAL RELEVANCE: ML-1,785,713 is a novel, potent COX-2 inhibitor that is the most selective COX-2 inhibitor described for use in dogs to date. ML-1,785,713 has oral bioavailability and low systemic clearance that is comparable to other non-steroidal anti-inflammatory drugs. It is effective after prophylactic and therapeutic administration in attenuating lameness in dogs with urate crystal-induced synovitis. Drugs that specifically inhibit COX-2 and not COX-1 at therapeutic doses may have an improved tolerability profile, compared with nonselective non-steroidal anti-inflammatory drugs.     

体外评定酸化剂作用效果的方法探讨

文章主要论述了体外法评定酸化剂作用效果的指标和评定方法。     

2007年第一季度饲料和原料中霉菌毒素调查报告

1样品概述 2007年1－3月,百奥明公司共收集来自全国各地的样本68份,其中：玉米28份,配合饲料样本34份,DDGS、豆粕、棉粕、菜粕、花生粕及玉米蛋白粉各1份。[第一段]     

CXCL8((3-73))K11R/G31P antagonizes the neutrophil chemoattractants present in pasteurellosis and mastitis lesions and abrogates neutrophil influx into intradermal endotoxin challenge sites in vivo

Toxic products such as reactive oxygen intermediates released by activated polymorphonuclear neutrophil (PMN) have an important role in the pathophysiology of diseases associated with the deposition of immune complexes (IC) in tissues. IC-induced activation of PMN requires adhesion mediated by integrin adhesion receptors. Of the integrins expressed on PMN, the beta(2) family has been found to be of particular importance for activation of PMN by IC. beta(2) Integrin ligand binding must be activated to enable adhesion to IC. Both activating and inhibitory signals regulate beta(2) integrin ligand avidity and adhesion. The second messenger cyclic adenosine monophosphate (cAMP) has been demonstrated to inhibit the activation of PMN in response to a variety of stimuli. The purpose of this study is to test the hypothesis that cAMP-dependent signals inhibit beta(2) integrin-dependent adhesion of equine PMN to immobilized IC and subsequent adhesion-dependent activation of respiratory burst activity. Treatment of equine PMN with beta(2) adrenergic agonists isoproterenol or clenbuterol, which trigger an increase in intracellular cAMP concentration, inhibited adhesion of equine PMN to IC in a dose dependent manner. Similarly, inhibition of cAMP hydrolysis by the non-specific phosphodiesterase (PDE) inhibitor pentoxifylline and the PDE 4-specific inhibitor rolipram inhibited adhesion of equine PMN to IC. Elevation of intracellular cAMP levels with pentoxifylline, clenbuterol and rolipram also inhibited IC-induced activation of respiratory burst activity in equine PMN. Importantly, co-treatment of equine PMN with rolipram and either beta(2) adrenergic agonist synergistically inhibited both the adhesion of equine PMN to IC as well as the subsequent respiratory burst activity.